{"id":"https://openalex.org/W7212005493","doi":"https://doi.org/10.64898/2026.09.04.749277","title":"Allosteric remodelling of the Ebola virus glycoprotein underlies differences in entry mechanisms across species","display_name":"Allosteric remodelling of the Ebola virus glycoprotein underlies differences in entry mechanisms across species","publication_year":2026,"publication_date":"2026-09-08","ids":{"openalex":"https://openalex.org/W7212005493","doi":"https://doi.org/10.64898/2026.09.04.749277"},"language":"en","primary_location":{"id":"doi:10.64898/2026.09.04.749277","doi":"https://doi.org/10.64898/2026.09.04.749277","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.04.749277","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.09.04.749277","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056327191","display_name":"Valeria Calvaresi","orcid":"https://orcid.org/0000-0002-1756-8853","observed_orcids":["https://orcid.org/0000-0002-1756-8853"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Valeria Calvaresi","raw_affiliation_strings":["Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK"],"raw_orcid":"https://orcid.org/0000-0002-1756-8853","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024724761","display_name":"Ji\u0159\u00ed Kratochv\u00edl","orcid":"https://orcid.org/0000-0003-1282-1562","observed_orcids":["https://orcid.org/0000-0003-1282-1562"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ji\u0159\u00ed Kratochv\u00edl","raw_affiliation_strings":["Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK","The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK"],"raw_orcid":"https://orcid.org/0000-0003-1282-1562","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5150928343","display_name":"Anna Bekauri","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Anna Bekauri","raw_affiliation_strings":["Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025711177","display_name":"Philipp Kukura","orcid":"https://orcid.org/0000-0003-0136-7704","observed_orcids":["https://orcid.org/0000-0003-0136-7704"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Philipp Kukura","raw_affiliation_strings":["Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK","The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK"],"raw_orcid":"https://orcid.org/0000-0003-0136-7704","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012415731","display_name":"Weston B. Struwe","orcid":"https://orcid.org/0000-0003-0594-226X","observed_orcids":["https://orcid.org/0000-0003-0594-226X"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Weston B. Struwe","raw_affiliation_strings":["Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK"],"raw_orcid":"https://orcid.org/0000-0003-0594-226X","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]},{"raw_affiliation_string":"The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5012415731"],"corresponding_institution_ids":["https://openalex.org/I40120149"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9545999765396118,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9545999765396118,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ebola-virus-glycoprotein","display_name":"Ebola virus glycoprotein","score":0.9580000042915344},{"id":"https://openalex.org/keywords/virus-entry","display_name":"viral entry","score":0.8119999766349792},{"id":"https://openalex.org/keywords/structural-dynamics","display_name":"structural dynamics","score":0.5680000185966492},{"id":"https://openalex.org/keywords/zaire-ebolavirus","display_name":"Zaire ebolavirus","score":0.7350000143051147},{"id":"https://openalex.org/keywords/species-differences","display_name":"species differences","score":0.6539999842643738},{"id":"https://openalex.org/keywords/endolysosomal-pathway","display_name":"endolysosomal pathway","score":0.8709999918937683}],"concepts":[{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.8873000144958496},{"id":"https://openalex.org/C2777469322","wikidata":"https://www.wikidata.org/wiki/Q8064876","display_name":"Ebola virus","level":3,"score":0.7064999938011169},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5839999914169312},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.49729999899864197},{"id":"https://openalex.org/C103038307","wikidata":"https://www.wikidata.org/wiki/Q6556360","display_name":"Lipid bilayer fusion","level":3,"score":0.49050000309944153},{"id":"https://openalex.org/C58475186","wikidata":"https://www.wikidata.org/wiki/Q4118894","display_name":"Viral entry","level":4,"score":0.4657999873161316},{"id":"https://openalex.org/C2909623084","wikidata":"https://www.wikidata.org/wiki/Q5331908","display_name":"Ebolavirus","level":4,"score":0.46399998664855957},{"id":"https://openalex.org/C156407911","wikidata":"https://www.wikidata.org/wiki/Q420160","display_name":"Dynamin","level":4,"score":0.4456000030040741},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.40389999747276306},{"id":"https://openalex.org/C81444415","wikidata":"https://www.wikidata.org/wiki/Q7243535","display_name":"Priming (agriculture)","level":3,"score":0.3806999921798706},{"id":"https://openalex.org/C2909439513","wikidata":"https://www.wikidata.org/wiki/Q18030682","display_name":"Cyclophilin A","level":2,"score":0.3747999966144562},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.358599990606308},{"id":"https://openalex.org/C2779574689","wikidata":"https://www.wikidata.org/wiki/Q2368907","display_name":"Arenavirus","level":5,"score":0.34529998898506165},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33880001306533813},{"id":"https://openalex.org/C2780034444","wikidata":"https://www.wikidata.org/wiki/Q5052485","display_name":"Cathepsin L","level":4,"score":0.33469998836517334},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.32179999351501465},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3199999928474426},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.3041999936103821},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.3003000020980835},{"id":"https://openalex.org/C2777339483","wikidata":"https://www.wikidata.org/wiki/Q647203","display_name":"Conformational change","level":2,"score":0.29089999198913574},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.28940001130104065},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.2825999855995178},{"id":"https://openalex.org/C191120209","wikidata":"https://www.wikidata.org/wiki/Q908902","display_name":"Structural biology","level":2,"score":0.27219998836517334},{"id":"https://openalex.org/C175969161","wikidata":"https://www.wikidata.org/wiki/Q323426","display_name":"Exocytosis","level":3,"score":0.2685000002384186},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26420000195503235},{"id":"https://openalex.org/C204641814","wikidata":"https://www.wikidata.org/wiki/Q899224","display_name":"Glycobiology","level":4,"score":0.2637999951839447},{"id":"https://openalex.org/C2777648638","wikidata":"https://www.wikidata.org/wiki/Q57751738","display_name":"Coronavirus","level":5,"score":0.2500999867916107}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.09.04.749277","doi":"https://doi.org/10.64898/2026.09.04.749277","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.04.749277","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.09.04.749277","doi":"https://doi.org/10.64898/2026.09.04.749277","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.09.04.749277","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4255831874","display_name":null,"funder_award_id":"UKRI Future Leaders Fellowship (MR/V02213X/1) to W.B.S.","funder_id":"https://openalex.org/F4320314731","funder_display_name":"UK Research and Innovation"},{"id":"https://openalex.org/G7831721387","display_name":null,"funder_award_id":"EPSRC grant EP/W001055/1 to P.K.","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320314731","display_name":"UK Research and Innovation","ror":"https://ror.org/001aqnf71"},{"id":"https://openalex.org/F4320334627","display_name":"Engineering and Physical Sciences Research Council","ror":"https://ror.org/0439y7842"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":63,"referenced_works":["https://openalex.org/W339361884","https://openalex.org/W1543832998","https://openalex.org/W1760776415","https://openalex.org/W1978996068","https://openalex.org/W1996037007","https://openalex.org/W1996334859","https://openalex.org/W2003408366","https://openalex.org/W2010823171","https://openalex.org/W2022339063","https://openalex.org/W2027824822","https://openalex.org/W2028570032","https://openalex.org/W2032086508","https://openalex.org/W2043674872","https://openalex.org/W2043829974","https://openalex.org/W2064369118","https://openalex.org/W2067080638","https://openalex.org/W2067510101","https://openalex.org/W2069796283","https://openalex.org/W2080374377","https://openalex.org/W2084313663","https://openalex.org/W2097231877","https://openalex.org/W2098345833","https://openalex.org/W2109176931","https://openalex.org/W2110951885","https://openalex.org/W2113132163","https://openalex.org/W2115102869","https://openalex.org/W2127440699","https://openalex.org/W2128963410","https://openalex.org/W2147679296","https://openalex.org/W2157754567","https://openalex.org/W2222266301","https://openalex.org/W2237639303","https://openalex.org/W2268380397","https://openalex.org/W2274022023","https://openalex.org/W2274478712","https://openalex.org/W2396636851","https://openalex.org/W2466466163","https://openalex.org/W2507513922","https://openalex.org/W2607309113","https://openalex.org/W2792198944","https://openalex.org/W2801919855","https://openalex.org/W2884117295","https://openalex.org/W2953861580","https://openalex.org/W2955168413","https://openalex.org/W3004859799","https://openalex.org/W3011289293","https://openalex.org/W3014406922","https://openalex.org/W3087347658","https://openalex.org/W3127683214","https://openalex.org/W3137409463","https://openalex.org/W3197273087","https://openalex.org/W4200095380","https://openalex.org/W4238969460","https://openalex.org/W4307841245","https://openalex.org/W4318225069","https://openalex.org/W4324138357","https://openalex.org/W4400180732","https://openalex.org/W4400378016","https://openalex.org/W4402697765","https://openalex.org/W4407071329","https://openalex.org/W4409197556","https://openalex.org/W4415126684","https://openalex.org/W7118910361"],"related_works":["https://openalex.org/W2028328803","https://openalex.org/W3004859799","https://openalex.org/W2032086508","https://openalex.org/W4256429498","https://openalex.org/W7098955076","https://openalex.org/W2953861580","https://openalex.org/W3204201748","https://openalex.org/W2098345833","https://openalex.org/W2237639303","https://openalex.org/W2583147894"],"abstract_inverted_index":{"Abstract":[0],"During":[1],"Ebola":[2],"virus":[3],"transit":[4],"through":[5],"the":[6,9,71,78,83],"endo-lysosomal":[7],"pathway,":[8],"glycoprotein":[10],"GP":[11,59,97,137],"undergoes":[12],"a":[13,115],"series":[14],"of":[15,58,60,77,82],"conformational":[16],"rearrangements":[17,32],"upon":[18,91],"cathepsin":[19,98],"cleavage":[20,79,99],"and":[21,48,55,66,87,100,106,110,130,140],"NPC1":[22,92],"receptor":[23,84,101],"binding,":[24],"culminating":[25],"in":[26,114,128,145],"host-virus":[27],"membrane":[28],"fusion.":[29],"While":[30],"these":[31],"underpin":[33],"viral":[34],"entry,":[35],"their":[36],"molecular":[37],"mechanisms":[38,105,147],"remain":[39],"poorly":[40],"understood.":[41],"Here,":[42],"we":[43],"combine":[44],"hydrogen/deuterium-exchange":[45],"mass":[46,49],"spectrometry":[47],"photometry":[50],"to":[51],"resolve":[52],"structural":[53,138],"dynamic":[54],"energetic":[56],"transitions":[57],"two":[61],"major":[62],"species,":[63,109],"Zaire":[64],"(EBOV)":[65],"Sudan":[67],"(SUDV).":[68],"We":[69,94,118],"describe":[70],"allosteric":[72,141],"axes":[73],"that":[74,96,120,136],"govern":[75],"reorganization":[76],"sites,":[80],"opening":[81],"binding":[85,102],"cavity":[86],"priming":[88,122],"for":[89],"fusion":[90,121],"engagement.":[93],"show":[95],"exhibit":[103],"different":[104],"kinetics":[107],"across":[108,148],"are":[111],"allosterically":[112],"coupled":[113],"species-specific":[116],"manner.":[117],"reveal":[119],"is":[123],"optimized":[124],"via":[125],"distinct":[126],"routes":[127],"EBOV":[129],"SUDV":[131],"GP.":[132],"These":[133],"data":[134],"suggest":[135],"dynamics":[139],"remodelling":[142],"underlie":[143],"differences":[144],"entry":[146],"Ebolavirus":[149],"species.":[150]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-10T00:00:00"}