{"id":"https://openalex.org/W7207625016","doi":"https://doi.org/10.1021/acs.analchem.6c03854","title":"Advancing Charge Detection Mass Spectrometry for Characterizing Large, Intact mRNA Constructs","display_name":"Advancing Charge Detection Mass Spectrometry for Characterizing Large, Intact mRNA Constructs","publication_year":2026,"publication_date":"2026-09-01","ids":{"openalex":"https://openalex.org/W7207625016","doi":"https://doi.org/10.1021/acs.analchem.6c03854","pmid":"https://pubmed.ncbi.nlm.nih.gov/42689509","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13584696"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.6c03854","doi":"https://doi.org/10.1021/acs.analchem.6c03854","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.6c03854","pdf_url":"https://pubs.acs.org/ancham/article-pdf/doi/10.1021/acs.analchem.6c03854/68105318/acs.analchem.6c03854.pdf","source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://pubs.acs.org/ancham/article-pdf/doi/10.1021/acs.analchem.6c03854/68105318/acs.analchem.6c03854.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5137912673","display_name":"Lohra M. Young","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lohra M. Young","raw_affiliation_strings":["Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064962658","display_name":"Benjamin E. Draper","orcid":"https://orcid.org/0000-0002-9727-2509","observed_orcids":["https://orcid.org/0000-0002-9727-2509"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Benjamin E. Draper","raw_affiliation_strings":["Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,"],"raw_orcid":"https://orcid.org/0000-0002-9727-2509","affiliations":[{"raw_affiliation_string":"Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5117304259","display_name":"Mark Chipley","orcid":"https://orcid.org/0009-0006-8429-0915","observed_orcids":["https://orcid.org/0009-0006-8429-0915"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Mark Chipley","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":"https://orcid.org/0009-0006-8429-0915","affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061503158","display_name":"Lauren F. Barnes","orcid":"https://orcid.org/0000-0003-1516-4211","observed_orcids":["https://orcid.org/0000-0003-1516-4211"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren F. Barnes","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073949139","display_name":"Brian C. Gau","orcid":"https://orcid.org/0000-0003-0770-5583","observed_orcids":["https://orcid.org/0000-0003-0770-5583"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian Gau","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003767403","display_name":"Andrew Dawdy","orcid":"https://orcid.org/0000-0003-1932-012X","observed_orcids":["https://orcid.org/0000-0003-1932-012X"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew Dawdy","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054874141","display_name":"Alex Kearns","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alex Kearns","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013860101","display_name":"John D. Orlet","orcid":"https://orcid.org/0000-0002-1748-0763","observed_orcids":["https://orcid.org/0000-0002-1748-0763"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Orlet","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017305191","display_name":"Thomas W. Powers","orcid":"https://orcid.org/0000-0003-1666-4592","observed_orcids":["https://orcid.org/0000-0003-1666-4592"]},"institutions":[{"id":"https://openalex.org/I180857899","display_name":"Pfizer (United States)","ror":"https://ror.org/01xdqrp08","country_code":"US","type":"company","lineage":["https://openalex.org/I180857899"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas W. Powers","raw_affiliation_strings":["Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotherapeutics Pharmaceutical Sciences, Pfizer , , , ,","institution_ids":["https://openalex.org/I180857899"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063887646","display_name":"Martin F. Jarrold","orcid":"https://orcid.org/0000-0001-7084-176X","observed_orcids":["https://orcid.org/0000-0001-7084-176X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Martin F. Jarrold","raw_affiliation_strings":["Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Megadalton Solutions Inc , 3750 E Bluebird Ln , , ,","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5117304259"],"corresponding_institution_ids":["https://openalex.org/I180857899"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":{"value":4500,"currency":"USD","value_usd":4500},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.51233658,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"98","issue":"36","first_page":"26988","last_page":"26997"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/charge-detection-mass-spectrometry","display_name":"charge detection mass spectrometry","score":0.9900000095367432},{"id":"https://openalex.org/keywords/mrna-vaccines","display_name":"mRNA vaccines","score":0.4180000126361847},{"id":"https://openalex.org/keywords/5-cap","display_name":"5' cap","score":0.8809999823570251},{"id":"https://openalex.org/keywords/poly-a-tail","display_name":"poly(A) tail","score":0.9300000071525574},{"id":"https://openalex.org/keywords/nucleotide-modification","display_name":"nucleotide modification","score":0.8059999942779541},{"id":"https://openalex.org/keywords/lipid-nanoparticles","display_name":"lipid nanoparticles","score":0.8209999799728394},{"id":"https://openalex.org/keywords/mrna-stability","display_name":"mRNA stability","score":0.6539999842643738}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8396000266075134},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.8061000108718872},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6205000281333923},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.5217999815940857},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4009999930858612},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.391400009393692},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3522999882698059},{"id":"https://openalex.org/C2778692840","wikidata":"https://www.wikidata.org/wiki/Q9136673","display_name":"Hemagglutinin (influenza)","level":3,"score":0.335999995470047},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.33329999446868896},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.3230000138282776},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3127000033855438},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3018999993801117},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.2883000075817108},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2833999991416931},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.27639999985694885},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.2700999975204468},{"id":"https://openalex.org/C2778576202","wikidata":"https://www.wikidata.org/wiki/Q2540164","display_name":"Sample preparation","level":2,"score":0.26660001277923584},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2623000144958496},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.25940001010894775},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.25600001215934753}],"mesh":[{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019267","descriptor_name":"Hemagglutinin Glycoproteins, Influenza Virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/acs.analchem.6c03854","doi":"https://doi.org/10.1021/acs.analchem.6c03854","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.6c03854","pdf_url":"https://pubs.acs.org/ancham/article-pdf/doi/10.1021/acs.analchem.6c03854/68105318/acs.analchem.6c03854.pdf","source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:42689509","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42689509","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13584696","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13584696/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Anal Chem","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1021/acs.analchem.6c03854","doi":"https://doi.org/10.1021/acs.analchem.6c03854","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.analchem.6c03854","pdf_url":"https://pubs.acs.org/ancham/article-pdf/doi/10.1021/acs.analchem.6c03854/68105318/acs.analchem.6c03854.pdf","source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.504800021648407,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7207625016.pdf","grobid_xml":"https://content.openalex.org/works/W7207625016.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1502957144","https://openalex.org/W1963706689","https://openalex.org/W1974321692","https://openalex.org/W2002025698","https://openalex.org/W2069464326","https://openalex.org/W2082181813","https://openalex.org/W2272801405","https://openalex.org/W2414460090","https://openalex.org/W2782708334","https://openalex.org/W2967571688","https://openalex.org/W2979778604","https://openalex.org/W3130729977","https://openalex.org/W3156114568","https://openalex.org/W3214423705","https://openalex.org/W3215511122","https://openalex.org/W4283033789","https://openalex.org/W4285391574","https://openalex.org/W4308293505","https://openalex.org/W4312204909","https://openalex.org/W4316039789","https://openalex.org/W4318779478","https://openalex.org/W4379107737","https://openalex.org/W4379259615","https://openalex.org/W4386391445","https://openalex.org/W4387002486","https://openalex.org/W4387242059","https://openalex.org/W4391958084","https://openalex.org/W4392122496","https://openalex.org/W4392793295","https://openalex.org/W4400674191","https://openalex.org/W4401632618","https://openalex.org/W4407724830","https://openalex.org/W4408597507","https://openalex.org/W4408717489","https://openalex.org/W4410472518","https://openalex.org/W7162080149","https://openalex.org/W7162681448"],"related_works":["https://openalex.org/W4408597507","https://openalex.org/W7220708385","https://openalex.org/W7162080149","https://openalex.org/W4406917187","https://openalex.org/W4410472518","https://openalex.org/W4313236460","https://openalex.org/W4383058377","https://openalex.org/W4391134258","https://openalex.org/W4386595091","https://openalex.org/W4388291501"],"abstract_inverted_index":{"Recent":[0],"advances":[1],"in":[2,35,199,255],"mRNA":[3,16,28,40,60,84,135,190],"(mRNA)":[4],"design":[5],"and":[6,29,38,49,94,117,204,229,268],"manufacturing":[7],"have":[8],"allowed":[9],"novel":[10],"approaches":[11],"to":[12,63,76,91,110,221,233,272],"fighting":[13],"disease.":[14,39],"When":[15],"is":[17,42,105,163],"administered":[18],"as":[19,121],"a":[20,106,185],"vaccine":[21],"the":[22,27,46,50,67,70,74,81,112,128,182,235,241,274,280],"host's":[23],"cell":[24],"machinery":[25],"reads":[26],"translates":[30],"into":[31,140,264],"proteins":[32],"that":[33,142,167,253],"aid":[34],"preventing":[36],"infection":[37],"translation":[41],"tightly":[43],"regulated,":[44],"with":[45,211,228],"5'":[47,57,62,77],"cap":[48,58],"poly(A)":[51,71,256],"tail":[52,72,257],"playing":[53],"important":[54],"roles.":[55],"The":[56],"protects":[59],"from":[61,219],"3'":[64,75],"exoribonucleases,":[65],"while":[66],"length":[68,209],"of":[69,83,99,114,131,175,184,214,237,276],"determines":[73],"exonucleolytic":[78],"decay.":[79],"As":[80],"use":[82],"treatments":[85],"expands,":[86],"analytical":[87],"techniques":[88],"are":[89,126],"required":[90],"confirm":[92,111],"identity":[93,113],"stability":[95],"at":[96],"every":[97],"stage":[98],"their":[100],"development.":[101],"Mass":[102],"spectrometry":[103,161],"(MS)":[104],"robust":[107],"technique":[108,166],"used":[109],"small":[115],"molecules":[116],"traditional":[118],"biotherapeutics":[119],"such":[120],"monoclonal":[122],"antibodies,":[123],"but":[124],"mRNAs":[125,197,248,261],"beyond":[127],"size":[129],"range":[130],"conventional":[132,147],"MS.":[133],"While":[134],"can":[136,143,155],"be":[137,144,156],"enzymatically":[138],"cleaved":[139],"fragments":[141],"measured":[145,242],"by":[146],"MS,":[148],"information":[149],"on":[150,240,279],"truncations":[151],"and/or":[152],"partial":[153],"sequences":[154],"lost.":[157],"Charge":[158],"detection":[159],"mass":[160,173],"(CDMS)":[162],"an":[164,212],"emerging":[165],"has":[168],"shown":[169],"significant":[170],"utility":[171],"for":[172,188,196],"measurements":[174],"large":[176],"heterogeneous":[177],"biomolecules.":[178],"Herein,":[179],"we":[180],"describe":[181],"development":[183],"CDMS":[186,245],"method":[187],"intact":[189,208],"analysis.":[191],"Sample":[192],"preparation":[193],"was":[194],"optimized":[195],"varying":[198],"size,":[200],"containing":[201],"both":[202,227],"natural":[203],"modified":[205],"bases,":[206],"giving":[207],"determination":[210],"uncertainty":[213],"0.5%.":[215],"Four":[216],"constructs,":[217],"ranging":[218],"997":[220],"4522":[222],"nts":[223],"(nucleotides),":[224],"were":[225,262],"examined":[226],"without":[230],"methoxyuridine":[231],"substitution":[232],"assess":[234,273],"impact":[236],"nucleotide":[238],"modification":[239],"mass.":[243],"Furthermore,":[244],"successfully":[246],"resolved":[247],"encoding":[249],"influenza":[250],"hemagglutinin":[251],"(HA)":[252],"varied":[254],"length.":[258],"Finally,":[259],"HA":[260],"encapsulated":[263],"lipid":[265],"nanoparticles":[266],"(LNPs)":[267],"reanalyzed":[269],"following":[270],"extraction":[271],"effects":[275],"LNP":[277],"packaging":[278],"mRNA.":[281]},"counts_by_year":[],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2026-09-04T00:00:00"}