{"id":"https://openalex.org/W7205015982","doi":"https://doi.org/10.64898/2026.08.28.747835","title":"Rapid repurposing of microvillar content drives a flagellate-to-amoeboid switch in the closest relative of animals","display_name":"Rapid repurposing of microvillar content drives a flagellate-to-amoeboid switch in the closest relative of animals","publication_year":2026,"publication_date":"2026-09-01","ids":{"openalex":"https://openalex.org/W7205015982","doi":"https://doi.org/10.64898/2026.08.28.747835"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.28.747835","doi":"https://doi.org/10.64898/2026.08.28.747835","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.28.747835","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.08.28.747835","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5119567370","display_name":"Maite Freire-Delgado","orcid":"https://orcid.org/0009-0003-2344-750X","observed_orcids":["https://orcid.org/0009-0003-2344-750X"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Maite Freire-Delgado","raw_affiliation_strings":["Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0009-0003-2344-750X","affiliations":[{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064512497","display_name":"Diede de Haan","orcid":"https://orcid.org/0000-0002-9793-1260","observed_orcids":["https://orcid.org/0000-0002-9793-1260"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4210111092","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/01zjc6908","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210111092","https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Diede de Haan","raw_affiliation_strings":["European Molecular Biology Laboratory, Grenoble, France","Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-9793-1260","affiliations":[{"raw_affiliation_string":"European Molecular Biology Laboratory, Grenoble, France","institution_ids":["https://openalex.org/I4210111092"]},{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093283185","display_name":"St\u00e9phane Tachon","orcid":"https://orcid.org/0009-0000-2187-6333","observed_orcids":["https://orcid.org/0009-0000-2187-6333"]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"St\u00e9phane Tachon","raw_affiliation_strings":["Institut Pasteur, NanoImaging Core Facility, Centre de Ressources et Recherches Technologiques (C2RT), Paris, France"],"raw_orcid":"https://orcid.org/0009-0000-2187-6333","affiliations":[{"raw_affiliation_string":"Institut Pasteur, NanoImaging Core Facility, Centre de Ressources et Recherches Technologiques (C2RT), Paris, France","institution_ids":["https://openalex.org/I157536573"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020191149","display_name":"Anastasia D. Gazi","orcid":"https://orcid.org/0000-0002-2922-3625","observed_orcids":["https://orcid.org/0000-0002-2922-3625"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4387156267","display_name":"Biologie Mol\u00e9culaire Structurale et Processus Infectieux","ror":"https://ror.org/009b91528","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4387156267"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Anastasia D. Gazi","raw_affiliation_strings":["Ultrastructural BioImaging, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3528, 75015, Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-2922-3625","affiliations":[{"raw_affiliation_string":"Ultrastructural BioImaging, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3528, 75015, Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4387156267"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107021071","display_name":"Mylan Ansel","orcid":"https://orcid.org/0009-0003-5650-5956","observed_orcids":["https://orcid.org/0009-0003-5650-5956"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Mylan Ansel","raw_affiliation_strings":["Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0009-0003-5650-5956","affiliations":[{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033511487","display_name":"Eva K. Pillai","orcid":"https://orcid.org/0000-0002-4662-3356","observed_orcids":["https://orcid.org/0000-0002-4662-3356"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4210138560","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/03mstc592","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4210153543","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/050589e39","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["DE","FR"],"is_corresponding":false,"raw_author_name":"Eva K. Pillai","raw_affiliation_strings":["Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany","Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany","Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-4662-3356","affiliations":[{"raw_affiliation_string":"Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany","institution_ids":["https://openalex.org/I4210138560"]},{"raw_affiliation_string":"Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany","institution_ids":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]},{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086252580","display_name":"Marvin Albert","orcid":"https://orcid.org/0000-0003-2536-2545","observed_orcids":["https://orcid.org/0000-0003-2536-2545"]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH","FR"],"is_corresponding":false,"raw_author_name":"Marvin Albert","raw_affiliation_strings":["ETH Z\u00fcrich, D-BSSE Single Cell Facility, Basel, Switzerland","Institut Pasteur, Image Analysis Hub, Universit\u00e9 Paris Cit\u00e9, Paris, France"],"raw_orcid":"https://orcid.org/0000-0003-2536-2545","affiliations":[{"raw_affiliation_string":"ETH Z\u00fcrich, D-BSSE Single Cell Facility, Basel, Switzerland","institution_ids":["https://openalex.org/I35440088"]},{"raw_affiliation_string":"Institut Pasteur, Image Analysis Hub, Universit\u00e9 Paris Cit\u00e9, Paris, France","institution_ids":["https://openalex.org/I157536573","https://openalex.org/I204730241"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026733375","display_name":"Chantal Combredet","orcid":"https://orcid.org/0000-0002-2161-199X","observed_orcids":["https://orcid.org/0000-0002-2161-199X"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Chantal Combredet","raw_affiliation_strings":["Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-2161-199X","affiliations":[{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001756969","display_name":"Audrey Salles","orcid":"https://orcid.org/0000-0002-1890-6957","observed_orcids":["https://orcid.org/0000-0002-1890-6957"]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Audrey Salles","raw_affiliation_strings":["Photonic Bioimaging Unit (UTechS-PBI, C2RT), Centre de Ressources et Recherches Technologiques, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-1890-6957","affiliations":[{"raw_affiliation_string":"Photonic Bioimaging Unit (UTechS-PBI, C2RT), Centre de Ressources et Recherches Technologiques, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Paris, France","institution_ids":["https://openalex.org/I157536573","https://openalex.org/I204730241"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068316606","display_name":"Jean-Yves Tin\u00e9vez","orcid":"https://orcid.org/0000-0002-0998-4718","observed_orcids":["https://orcid.org/0000-0002-0998-4718"]},"institutions":[{"id":"https://openalex.org/I4210138560","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/03mstc592","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4210153543","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/050589e39","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jean-Yves Tinevez","raw_affiliation_strings":["Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany"],"raw_orcid":"https://orcid.org/0000-0002-0998-4718","affiliations":[{"raw_affiliation_string":"Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany","institution_ids":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012864821","display_name":"Thibaut Brunet","orcid":"https://orcid.org/0000-0002-1843-1613","observed_orcids":["https://orcid.org/0000-0002-1843-1613"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4405277297","display_name":"Biologie cellulaire physiologique et pathologique","ror":"https://ror.org/0267nwx23","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I4405277297"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Thibaut Brunet","raw_affiliation_strings":["Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France"],"raw_orcid":"https://orcid.org/0000-0002-1843-1613","affiliations":[{"raw_affiliation_string":"Institut Pasteur, Universit\u00e9 Paris-Cit\u00e9, CNRS UMR3691, Evolutionary Cell Biology and Evolution of Morphogenesis Unit, 25-28 Rue du Docteur Roux, 75015 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5012864821"],"corresponding_institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573","https://openalex.org/I204730241","https://openalex.org/I4405277297"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.6973000168800354,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.6973000168800354,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11879","display_name":"Protist diversity and phylogeny","score":0.13429999351501465,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.11379999667406082,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/choanoflagellates","display_name":"choanoflagellates","score":0.9909999966621399},{"id":"https://openalex.org/keywords/salpingoeca-rosetta","display_name":"Salpingoeca rosetta","score":0.9430000185966492},{"id":"https://openalex.org/keywords/microvilli","display_name":"microvilli","score":0.7519999742507935},{"id":"https://openalex.org/keywords/cytoskeletal-remodeling","display_name":"cytoskeletal remodeling","score":0.7860000133514404},{"id":"https://openalex.org/keywords/calcium-release","display_name":"calcium release","score":0.6940000057220459},{"id":"https://openalex.org/keywords/ezrin-radixin-moesin","display_name":"ezrin/radixin/moesin","score":0.8899999856948853},{"id":"https://openalex.org/keywords/cortical-microtubules","display_name":"cortical microtubules","score":0.9549999833106995},{"id":"https://openalex.org/keywords/membrane-internalization","display_name":"membrane internalization","score":0.5149999856948853}],"concepts":[{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7538999915122986},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.7487000226974487},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7088000178337097},{"id":"https://openalex.org/C204462481","wikidata":"https://www.wikidata.org/wiki/Q5058180","display_name":"Cell cortex","level":4,"score":0.6078000068664551},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.5360999703407288},{"id":"https://openalex.org/C58207958","wikidata":"https://www.wikidata.org/wiki/Q33596","display_name":"Motility","level":2,"score":0.515999972820282},{"id":"https://openalex.org/C87555872","wikidata":"https://www.wikidata.org/wiki/Q3141316","display_name":"Ultrastructure","level":2,"score":0.4936999976634979},{"id":"https://openalex.org/C20418707","wikidata":"https://www.wikidata.org/wiki/Q189933","display_name":"Microtubule","level":2,"score":0.47940000891685486},{"id":"https://openalex.org/C2777348757","wikidata":"https://www.wikidata.org/wiki/Q2346577","display_name":"Cortex (anatomy)","level":2,"score":0.412200003862381},{"id":"https://openalex.org/C3620293","wikidata":"https://www.wikidata.org/wiki/Q14908027","display_name":"Actin remodeling","level":5,"score":0.398499995470047},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.3977000117301941},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.36070001125335693},{"id":"https://openalex.org/C151000934","wikidata":"https://www.wikidata.org/wiki/Q4676834","display_name":"Actin remodeling of neurons","level":5,"score":0.3587999939918518},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.31940001249313354},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.3125},{"id":"https://openalex.org/C2993775838","wikidata":"https://www.wikidata.org/wiki/Q14943002","display_name":"Video microscopy","level":2,"score":0.2955000102519989},{"id":"https://openalex.org/C115811362","wikidata":"https://www.wikidata.org/wiki/Q189998","display_name":"Flagellum","level":3,"score":0.2939999997615814},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.2833999991416931},{"id":"https://openalex.org/C85813293","wikidata":"https://www.wikidata.org/wiki/Q188909","display_name":"Cell division","level":3,"score":0.2777999937534332},{"id":"https://openalex.org/C181080969","wikidata":"https://www.wikidata.org/wiki/Q60097","display_name":"Calcium in biology","level":3,"score":0.2551000118255615}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.28.747835","doi":"https://doi.org/10.64898/2026.08.28.747835","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.28.747835","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.28.747835","doi":"https://doi.org/10.64898/2026.08.28.747835","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.28.747835","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":94,"referenced_works":["https://openalex.org/W1560372748","https://openalex.org/W1599277155","https://openalex.org/W1975149361","https://openalex.org/W1976140509","https://openalex.org/W1979366429","https://openalex.org/W1981601881","https://openalex.org/W1988278060","https://openalex.org/W1995201958","https://openalex.org/W1997190969","https://openalex.org/W2007399351","https://openalex.org/W2018943463","https://openalex.org/W2032100183","https://openalex.org/W2035823944","https://openalex.org/W2050520583","https://openalex.org/W2055567387","https://openalex.org/W2072977772","https://openalex.org/W2086064567","https://openalex.org/W2094838913","https://openalex.org/W2100338747","https://openalex.org/W2103783368","https://openalex.org/W2110153797","https://openalex.org/W2113401723","https://openalex.org/W2118938838","https://openalex.org/W2129959101","https://openalex.org/W2140588274","https://openalex.org/W2147234043","https://openalex.org/W2163010743","https://openalex.org/W2167279371","https://openalex.org/W2180557564","https://openalex.org/W2340685884","https://openalex.org/W2529052661","https://openalex.org/W2605863429","https://openalex.org/W2614029615","https://openalex.org/W2680338049","https://openalex.org/W2735451764","https://openalex.org/W2793567263","https://openalex.org/W2802432105","https://openalex.org/W2883029425","https://openalex.org/W2894721371","https://openalex.org/W2896243061","https://openalex.org/W2904229723","https://openalex.org/W2958578978","https://openalex.org/W2971089545","https://openalex.org/W2980718989","https://openalex.org/W2981757002","https://openalex.org/W3000999925","https://openalex.org/W3015290071","https://openalex.org/W3024073578","https://openalex.org/W3028033347","https://openalex.org/W3038485847","https://openalex.org/W3083406432","https://openalex.org/W3088126680","https://openalex.org/W3093148868","https://openalex.org/W3093443465","https://openalex.org/W3104572769","https://openalex.org/W3109754928","https://openalex.org/W3111521801","https://openalex.org/W3123095577","https://openalex.org/W3126104802","https://openalex.org/W3129515806","https://openalex.org/W3214976122","https://openalex.org/W4225759392","https://openalex.org/W4229457981","https://openalex.org/W4230594613","https://openalex.org/W4309176870","https://openalex.org/W4318767730","https://openalex.org/W4320710123","https://openalex.org/W4366084204","https://openalex.org/W4375948977","https://openalex.org/W4385392723","https://openalex.org/W4389894034","https://openalex.org/W4390782971","https://openalex.org/W4393229199","https://openalex.org/W4394567285","https://openalex.org/W4398201169","https://openalex.org/W4401460076","https://openalex.org/W4402283320","https://openalex.org/W4404105423","https://openalex.org/W4404258185","https://openalex.org/W4405028556","https://openalex.org/W4405632481","https://openalex.org/W4406166345","https://openalex.org/W4406214214","https://openalex.org/W4413048738","https://openalex.org/W4413753144","https://openalex.org/W4414056776","https://openalex.org/W4414457840","https://openalex.org/W4414747926","https://openalex.org/W4415740969","https://openalex.org/W7162001020","https://openalex.org/W7164823763","https://openalex.org/W7166815741","https://openalex.org/W7220847701","https://openalex.org/W7221032271"],"related_works":["https://openalex.org/W1997518593","https://openalex.org/W2951473001","https://openalex.org/W2301268017","https://openalex.org/W4309176870","https://openalex.org/W2048794383","https://openalex.org/W4414056776","https://openalex.org/W2545389612","https://openalex.org/W2481764992","https://openalex.org/W2039975910","https://openalex.org/W2043986357"],"abstract_inverted_index":{"Abstract":[0],"Animal":[1],"cells":[2],"extensively":[3],"remodel":[4],"their":[5,43],"cytoskeleton":[6,230],"during":[7],"differentiation":[8],"and":[9,20,34,46,68,100,121,134,142,165,203,218,229],"can":[10,41],"notably":[11],"switch":[12],"between":[13],"two":[14],"major":[15],"motility":[16],"modes:":[17],"flagellum-based":[18],"swimming":[19],"actin-based":[21],"crawling.":[22],"We":[23],"previously":[24],"showed":[25],"that":[26],"choanoflagellates,":[27],"the":[28,82,127,139,166,172,176,180,213],"closest":[29],"living":[30],"relatives":[31],"of":[32,57,85,123,138,144,175,216,227],"animals":[33],"classically":[35],"viewed":[36],"as":[37,81,225],"obligate":[38],"flagellated":[39],"swimmers,":[40],"retract":[42],"collar":[44],"complex":[45],"adopt":[47],"an":[48,97,220],"amoeboid":[49,157],"form":[50],"within":[51],"seconds":[52],"under":[53,184],"spatial":[54],"confinement,":[55],"independently":[56],"regulated":[58],"gene":[59],"expression.":[60],"Here,":[61],"using":[62],"live":[63],"imaging,":[64],"ultrastructural":[65,83,210],"expansion":[66],"microscopy,":[67],"cryo-electron":[69],"tomography":[70,187],"in":[71,212],"Salpingoeca":[72],"rosetta":[73],",":[74],"we":[75],"identify":[76],"rapid,":[77],"cell-wide":[78],"cytoskeletal":[79,214],"remodeling":[80],"basis":[84],"this":[86],"switch.":[87],"Unconfined":[88],"choanoflagellates":[89,217],"lack":[90],"a":[91,197],"detectable":[92],"actin":[93,150,190],"cortex":[94,151],"but":[95],"display":[96],"apical":[98],"flagellum":[99],"cortical":[101,161,198],"microtubules,":[102],"with":[103],"F-actin":[104,141],"being":[105],"largely":[106],"restricted":[107],"to":[108,182,196,231],"microvilli.":[109],"Confinement":[110],"triggers":[111],"calcium":[112],"release":[113],"from":[114,192],"intracellular":[115],"stores,":[116],"which":[117,153],"induces":[118],"microvillar":[119,124,168,194],"retraction":[120],"absorption":[122],"material":[125],"into":[126],"cell":[128,177,181,233],"body,":[129,178],"including":[130],"actin,":[131],"ezrin-radixin-moesin":[132],"1,":[133],"plasma":[135,169],"membrane.":[136],"Remodeling":[137],"internalized":[140,193],"repurposing":[143],"associated":[145],"proteins":[146],"supports":[147,219],"de":[148],"novo":[149],"formation,":[152],"is":[154],"necessary":[155],"for":[156,223],"motility.":[158],"In":[159],"parallel,":[160],"microtubules":[162],"are":[163],"disassembled,":[164],"reabsorbed":[167],"membrane":[170,228],"increases":[171],"surface":[173],"area":[174],"allowing":[179],"flatten":[183],"confinement.":[185],"Cryo-electron":[186],"reveals":[188,208],"stepwise":[189],"reorganization":[191],"bundles":[195,202],"contractile":[199],"meshwork":[200],"combining":[201],"scattered":[204],"filaments.":[205],"This":[206],"work":[207],"considerable":[209],"plasticity":[211],"architecture":[215],"ancestral":[221],"role":[222],"microvilli":[224],"reservoirs":[226],"potentiate":[232],"phenotypic":[234],"transitions.":[235]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-09-02T00:00:00"}