{"id":"https://openalex.org/W7204879818","doi":"https://doi.org/10.56557/jafsat/2026/v13i411056","title":"CRISPR and Next-generation Molecular Diagnostics for Foodborne Pathogen Detection: Analytical Performance, Matrix Robustness and Translational Readiness","display_name":"CRISPR and Next-generation Molecular Diagnostics for Foodborne Pathogen Detection: Analytical Performance, Matrix Robustness and Translational Readiness","publication_year":2026,"publication_date":"2026-09-01","ids":{"openalex":"https://openalex.org/W7204879818","doi":"https://doi.org/10.56557/jafsat/2026/v13i411056"},"language":"en","primary_location":{"id":"doi:10.56557/jafsat/2026/v13i411056","doi":"https://doi.org/10.56557/jafsat/2026/v13i411056","is_oa":true,"landing_page_url":"https://doi.org/10.56557/jafsat/2026/v13i411056","pdf_url":null,"source":{"id":"https://openalex.org/S4387282282","display_name":"Journal of Advances in Food Science & Technology","issn_l":"2454-4213","issn":["2454-4213"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Food Science & Technology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.56557/jafsat/2026/v13i411056","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5139669408","display_name":"Nkemdilim Clairelouise Okechukwu","orcid":"https://orcid.org/0009-0001-0690-4663","observed_orcids":["https://orcid.org/0009-0001-0690-4663"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nkemdilim Clairelouise Okechukwu","raw_affiliation_strings":["Department of Medical Microbiology, Enugu State University Teaching Hospital, Parklane, Enugu, Nigeria"],"raw_orcid":"https://orcid.org/0009-0001-0690-4663","affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Enugu State University Teaching Hospital, Parklane, Enugu, Nigeria","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148858749","display_name":"Chizoba Victoria Onaha","orcid":"https://orcid.org/0009-0007-1517-8054","observed_orcids":["https://orcid.org/0009-0007-1517-8054"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chizoba Victoria Onah","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0007-1517-8054","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5137326073","display_name":"Adebola Ayisat Effa","orcid":"https://orcid.org/0009-0006-1076-5500","observed_orcids":["https://orcid.org/0009-0006-1076-5500"]},"institutions":[{"id":"https://openalex.org/I4405259095","display_name":"Nigeria Natural Medicine Development Agency","ror":"https://ror.org/01qpwsp84","country_code":"NG","type":"government","lineage":["https://openalex.org/I2801477186","https://openalex.org/I4210122850","https://openalex.org/I4405259095"]}],"countries":["NG"],"is_corresponding":false,"raw_author_name":"Adebola Ayisat Effa","raw_affiliation_strings":["Department of Product Development and Quality Assurance, Nigeria Natural Medicine Development Agency, Lagos, Nigeria"],"raw_orcid":"https://orcid.org/0009-0006-1076-5500","affiliations":[{"raw_affiliation_string":"Department of Product Development and Quality Assurance, Nigeria Natural Medicine Development Agency, Lagos, Nigeria","institution_ids":["https://openalex.org/I4405259095"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"13","issue":"4","first_page":"1","last_page":"21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9390000104904175,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9390000104904175,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.03009999915957451,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10900","display_name":"Listeria monocytogenes in Food Safety","score":0.009999999776482582,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-based-diagnostics","display_name":"CRISPR-based diagnostics","score":0.9819999933242798},{"id":"https://openalex.org/keywords/foodborne-pathogens","display_name":"foodborne pathogens","score":0.8759999871253967},{"id":"https://openalex.org/keywords/crispr-cas12a","display_name":"CRISPR/Cas12a","score":0.7459999918937683},{"id":"https://openalex.org/keywords/cas13","display_name":"Cas13","score":0.9829999804496765},{"id":"https://openalex.org/keywords/isothermal-amplification","display_name":"isothermal amplification","score":0.9190000295639038},{"id":"https://openalex.org/keywords/food-safety-testing","display_name":"food safety testing","score":0.43799999356269836},{"id":"https://openalex.org/keywords/detection-limit","display_name":"detection limit","score":0.6700000166893005},{"id":"https://openalex.org/keywords/multiplexing","display_name":"multiplexing","score":0.6029999852180481},{"id":"https://openalex.org/keywords/contamination-control","display_name":"contamination control","score":0.8220000267028809},{"id":"https://openalex.org/keywords/digital-pcr","display_name":"digital PCR","score":0.5920000076293945},{"id":"https://openalex.org/keywords/whole-genome-sequencing","display_name":"whole genome sequencing","score":0.9679999947547913},{"id":"https://openalex.org/keywords/metagenomics","display_name":"metagenomics","score":0.9459999799728394},{"id":"https://openalex.org/keywords/nanopore-sequencing","display_name":"nanopore sequencing","score":0.9750000238418579},{"id":"https://openalex.org/keywords/sample-preparation","display_name":"sample preparation","score":0.4320000112056732},{"id":"https://openalex.org/keywords/outbreak-response","display_name":"outbreak response","score":0.5009999871253967}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8716999888420105},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.7353000044822693},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.6543999910354614},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.5582000017166138},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.5336999893188477},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.5134000182151794},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.510200023651123},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.503600001335144},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.40130001306533813},{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.3953000009059906},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.3871000111103058},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3792000114917755},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.3359000086784363},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.33379998803138733},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.3181000053882599},{"id":"https://openalex.org/C126513998","wikidata":"https://www.wikidata.org/wiki/Q8973627","display_name":"Nanopore sequencing","level":4,"score":0.31610000133514404},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.3154999911785126},{"id":"https://openalex.org/C2779695955","wikidata":"https://www.wikidata.org/wiki/Q3869370","display_name":"NASBA","level":4,"score":0.3089999854564667},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2786000072956085},{"id":"https://openalex.org/C10590036","wikidata":"https://www.wikidata.org/wiki/Q417664","display_name":"Exome","level":5,"score":0.27079999446868896},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.26739999651908875},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26190000772476196},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2556999921798706},{"id":"https://openalex.org/C44667518","wikidata":"https://www.wikidata.org/wiki/Q12321","display_name":"Palindrome","level":4,"score":0.25369998812675476}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.56557/jafsat/2026/v13i411056","doi":"https://doi.org/10.56557/jafsat/2026/v13i411056","is_oa":true,"landing_page_url":"https://doi.org/10.56557/jafsat/2026/v13i411056","pdf_url":null,"source":{"id":"https://openalex.org/S4387282282","display_name":"Journal of Advances in Food Science & Technology","issn_l":"2454-4213","issn":["2454-4213"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Food Science & Technology","raw_type":"journal-article"},{"id":"pmh:oai:ikprress.org:article/11056","doi":null,"is_oa":true,"landing_page_url":"https://ikprress.org/index.php/JAFSAT/article/view/11056","pdf_url":null,"source":{"id":"https://openalex.org/S4387282282","display_name":"Journal of Advances in Food Science & Technology","issn_l":"2454-4213","issn":["2454-4213"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2454-4213","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:HAL:hal-05736738v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-05736738","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Advances in Food Science & Technology, 2026, 13 (4), pp.1-21","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.56557/jafsat/2026/v13i411056","doi":"https://doi.org/10.56557/jafsat/2026/v13i411056","is_oa":true,"landing_page_url":"https://doi.org/10.56557/jafsat/2026/v13i411056","pdf_url":null,"source":{"id":"https://openalex.org/S4387282282","display_name":"Journal of Advances in Food Science & Technology","issn_l":"2454-4213","issn":["2454-4213"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Food Science & Technology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4409911744","https://openalex.org/W6921275895","https://openalex.org/W6902902060","https://openalex.org/W7154942235","https://openalex.org/W7160650811","https://openalex.org/W4310950321","https://openalex.org/W7212277742","https://openalex.org/W7160497862","https://openalex.org/W4405462515","https://openalex.org/W4362584853"],"abstract_inverted_index":{"Foodborne-pathogen":[0],"testing":[1,89],"increasingly":[2],"requires":[3],"results":[4],"that":[5,116,220],"are":[6,150,165],"not":[7],"only":[8],"analytically":[9],"sensitive":[10],"but":[11],"also":[12],"rapid,":[13],"interpretable,":[14],"robust":[15],"in":[16,86,190,237],"heterogeneous":[17],"matrices":[18],"and":[19,27,75,122,130,138,158,160,176,187,209,216,248],"compatible":[20],"with":[21,47,80,106,244],"decisions":[22],"made":[23],"across":[24,127,168],"production,":[25],"surveillance":[26],"outbreak":[28],"response.":[29],"Clustered":[30],"regularly":[31],"interspaced":[32],"short":[33],"palindromic":[34],"repeats":[35],"(CRISPR)-associated":[36],"diagnostics":[37,61],"have":[38],"expanded":[39],"this":[40],"landscape":[41],"by":[42],"coupling":[43],"programmable":[44],"sequence":[45],"recognition":[46],"collateral":[48],"nucleic-acid":[49],"cleavage,":[50],"frequently":[51],"after":[52],"isothermal":[53,67],"amplification.":[54],"This":[55],"critical":[56],"narrative":[57],"review":[58],"evaluates":[59],"CRISPR":[60,137,222,240],"alongside":[62],"quantitative":[63,252],"polymerase":[64,70],"chain":[65,71],"reaction,":[66,72],"amplification,":[68,159],"digital":[69,136],"whole-genome":[73,207],"sequencing":[74,208,256],"metagenomic":[76],"or":[77,255],"nanopore":[78],"approaches,":[79],"emphasis":[81],"on":[82,153,266,273],"what":[83],"determines":[84],"performance":[85],"real":[87],"food":[88],"rather":[90],"than":[91,197,272],"purified-template":[92],"experiments.":[93],"Literature":[94],"published":[95],"from":[96],"1":[97],"January":[98],"2017":[99],"to":[100,250,258],"19":[101],"June":[102],"2026":[103],"was":[104],"considered,":[105],"earlier":[107],"foundational":[108],"studies":[109,169],"retained":[110],"where":[111],"necessary.":[112],"The":[113,226],"evidence":[114],"indicates":[115],"Cas12a-centred":[117],"assays":[118,223],"can":[119],"provide":[120,211],"rapid":[121,239],"highly":[123],"specific":[124],"targeted":[125,221],"detection":[126,164,270],"several":[128],"bacterial":[129],"viral":[131],"hazards,":[132],"while":[133],"Cas13,":[134],"Cas12b,":[135],"alternative":[139],"readouts":[140],"broaden":[141],"the":[142,146,259,277],"design":[143],"space.":[144],"Yet":[145],"apparent":[147],"analytical":[148],"gains":[149],"often":[151],"contingent":[152],"upstream":[154],"concentration,":[155],"enrichment,":[156],"lysis":[157],"reported":[161],"limits":[162,271],"of":[163,276],"poorly":[166],"comparable":[167],"because":[170],"units,":[171],"sample":[172,246],"inputs,":[173],"enrichment":[174],"histories":[175],"positivity":[177],"criteria":[178],"differ.":[179],"Viability":[180],"discrimination,":[181],"multiplexing,":[182],"contamination":[183],"control,":[184],"reagent":[185],"stability":[186],"prospective":[188],"validation":[189,275],"naturally":[191],"contaminated":[192],"foods":[193],"remain":[194],"less":[195,265],"mature":[196],"proof-of-concept":[198],"sensitivity.":[199],"Digital":[200],"PCR":[201],"offers":[202],"stronger":[203],"absolute":[204],"quantification,":[205],"whereas":[206],"metagenomics":[210],"strain":[212],"resolution,":[213],"source":[214],"attribution":[215],"broader":[217],"genomic":[218],"context":[219],"cannot":[224],"replace.":[225],"most":[227],"defensible":[228],"near-term":[229],"model":[230],"is":[231,242],"therefore":[232],"a":[233],"tiered":[234],"diagnostic":[235],"architecture":[236],"which":[238],"screening":[241],"integrated":[243],"fit-for-purpose":[245],"preparation":[247],"linked":[249],"culture,":[251],"molecular":[253],"confirmation":[254],"according":[257],"decision":[260],"required.":[261],"Translation":[262],"will":[263],"depend":[264],"progressively":[267],"lower":[268],"nominal":[269],"standardised":[274],"complete":[278],"sample-to-result":[279],"workflow.":[280]},"counts_by_year":[],"updated_date":"2026-10-25T09:29:13.331239","created_date":"2026-09-02T00:00:00"}