{"id":"https://openalex.org/W7204856617","doi":"https://doi.org/10.26434/chemrxiv.15008065/v1","title":"An activity\u2011based high\u2011throughput screening platform for identifying functional modulators of OFF\u2011type riboswitches","display_name":"An activity\u2011based high\u2011throughput screening platform for identifying functional modulators of OFF\u2011type riboswitches","publication_year":2026,"publication_date":"2026-08-31","ids":{"openalex":"https://openalex.org/W7204856617","doi":"https://doi.org/10.26434/chemrxiv.15008065/v1"},"language":"en","primary_location":{"id":"doi:10.26434/chemrxiv.15008065/v1","doi":"https://doi.org/10.26434/chemrxiv.15008065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008065/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008065/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008065/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065207562","display_name":"Vipul Navinchandra Panchal","orcid":"https://orcid.org/0000-0001-9776-1102","observed_orcids":["https://orcid.org/0000-0001-9776-1102"]},"institutions":[{"id":"https://openalex.org/I4432739","display_name":"University of Bergen","ror":"https://ror.org/03zga2b32","country_code":"NO","type":"education","lineage":["https://openalex.org/I4432739"]}],"countries":["NO"],"is_corresponding":false,"raw_author_name":"Vipul Panchal","raw_affiliation_strings":["Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway, 5020"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway, 5020","institution_ids":["https://openalex.org/I4432739"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093667806","display_name":"Michaela Lisova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210138103","display_name":"Czech Academy of Sciences, Institute of Molecular Genetics","ror":"https://ror.org/045syc608","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Michaela Lisov\u00e1","raw_affiliation_strings":["Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014915537","display_name":"Daniel A. Lafontaine","orcid":"https://orcid.org/0000-0001-7891-5700","observed_orcids":["https://orcid.org/0000-0001-7891-5700"]},"institutions":[{"id":"https://openalex.org/I135117807","display_name":"Universit\u00e9 de Sherbrooke","ror":"https://ror.org/00kybxq39","country_code":"CA","type":"education","lineage":["https://openalex.org/I135117807"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Daniel A. Lafontaine","raw_affiliation_strings":["RNA Group","Universit\u00e9 de Sherbrooke"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"RNA Group","institution_ids":[]},{"raw_affiliation_string":"Universit\u00e9 de Sherbrooke","institution_ids":["https://openalex.org/I135117807"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037913175","display_name":"Bengt Erik Haug","orcid":"https://orcid.org/0000-0003-3014-9538","observed_orcids":["https://orcid.org/0000-0003-3014-9538"]},"institutions":[{"id":"https://openalex.org/I4432739","display_name":"University of Bergen","ror":"https://ror.org/03zga2b32","country_code":"NO","type":"education","lineage":["https://openalex.org/I4432739"]}],"countries":["NO"],"is_corresponding":false,"raw_author_name":"Bengt Erik Haug","raw_affiliation_strings":["Department of Chemistry and Centre for Pharmacy, University of Bergen, All\u00e9gaten 41, Bergen, Norway, 5007"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Centre for Pharmacy, University of Bergen, All\u00e9gaten 41, Bergen, Norway, 5007","institution_ids":["https://openalex.org/I4432739"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047867480","display_name":"David Sedl\u00e1k","orcid":"https://orcid.org/0000-0003-0074-335X","observed_orcids":["https://orcid.org/0000-0003-0074-335X"]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210138103","display_name":"Czech Academy of Sciences, Institute of Molecular Genetics","ror":"https://ror.org/045syc608","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"David Sedlak","raw_affiliation_strings":["Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009238334","display_name":"Petr Bart\u016fn\u011bk","orcid":"https://orcid.org/0000-0002-6202-7650","observed_orcids":["https://orcid.org/0000-0002-6202-7650"]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210138103","display_name":"Czech Academy of Sciences, Institute of Molecular Genetics","ror":"https://ror.org/045syc608","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Petr Bartunek","raw_affiliation_strings":["Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, Prague, Czech Republic, 142 20","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210138103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043614478","display_name":"Matthias Mack","orcid":"https://orcid.org/0000-0002-7753-2422","observed_orcids":["https://orcid.org/0000-0002-7753-2422"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Matthias Mack","raw_affiliation_strings":["Institute for Technical Microbiology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Technical Microbiology","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5036784729","display_name":"Ruth Brenk","orcid":"https://orcid.org/0000-0002-6204-5488","observed_orcids":["https://orcid.org/0000-0002-6204-5488"]},"institutions":[{"id":"https://openalex.org/I4432739","display_name":"University of Bergen","ror":"https://ror.org/03zga2b32","country_code":"NO","type":"education","lineage":["https://openalex.org/I4432739"]}],"countries":["NO"],"is_corresponding":false,"raw_author_name":"Ruth Brenk","raw_affiliation_strings":["Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway, 5020"],"raw_orcid":"https://orcid.org/0000-0002-6204-5488","affiliations":[{"raw_affiliation_string":"Department of Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen, Norway, 5020","institution_ids":["https://openalex.org/I4432739"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/riboswitches","display_name":"riboswitches","score":0.9810000061988831},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7260000109672546},{"id":"https://openalex.org/keywords/read-through-transcription","display_name":"read-through transcription","score":0.8080000281333923},{"id":"https://openalex.org/keywords/molecular-beacons","display_name":"molecular beacons","score":0.40799999237060547},{"id":"https://openalex.org/keywords/in-vitro-transcription","display_name":"in vitro transcription","score":0.6940000057220459},{"id":"https://openalex.org/keywords/thiamine-pyrophosphate-riboswitch","display_name":"thiamine pyrophosphate riboswitch","score":0.9049999713897705},{"id":"https://openalex.org/keywords/small-molecule-modulators","display_name":"small molecule modulators","score":0.7319999933242798},{"id":"https://openalex.org/keywords/rna-targeted-drug-discovery","display_name":"RNA-targeted drug discovery","score":0.6330000162124634},{"id":"https://openalex.org/keywords/target-prioritization","display_name":"target prioritization","score":0.6570000052452087}],"concepts":[{"id":"https://openalex.org/C148625055","wikidata":"https://www.wikidata.org/wiki/Q418840","display_name":"Riboswitch","level":5,"score":0.9366000294685364},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.754800021648407},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.6581000089645386},{"id":"https://openalex.org/C10679952","wikidata":"https://www.wikidata.org/wiki/Q5308937","display_name":"Druggability","level":3,"score":0.6248999834060669},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.45829999446868896},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4242999851703644},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41990000009536743},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36980000138282776},{"id":"https://openalex.org/C26261996","wikidata":"https://www.wikidata.org/wiki/Q2382392","display_name":"Bacterial transcription","level":5,"score":0.36730000376701355},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3479999899864197},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.33329999446868896},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.32499998807907104},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.3075999915599823},{"id":"https://openalex.org/C103697762","wikidata":"https://www.wikidata.org/wiki/Q4112105","display_name":"Virtual screening","level":3,"score":0.2761000096797943},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.26759999990463257},{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.263700008392334},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.26249998807907104},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.2614000141620636},{"id":"https://openalex.org/C194993378","wikidata":"https://www.wikidata.org/wiki/Q427087","display_name":"Non-coding RNA","level":4,"score":0.2565999925136566}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.26434/chemrxiv.15008065/v1","doi":"https://doi.org/10.26434/chemrxiv.15008065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008065/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008065/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.26434/chemrxiv.15008065/v1","doi":"https://doi.org/10.26434/chemrxiv.15008065/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008065/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008065/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1272044255","display_name":null,"funder_award_id":"68378050-KAV-NPUI","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"},{"id":"https://openalex.org/G1937647480","display_name":null,"funder_award_id":"297192","funder_id":"https://openalex.org/F4320314161","funder_display_name":"Joint Programming Initiative on Antimicrobial Resistance"},{"id":"https://openalex.org/G2403481850","display_name":null,"funder_award_id":"297192","funder_id":"https://openalex.org/F4320323299","funder_display_name":"Norges Forskningsr\u00e5d"},{"id":"https://openalex.org/G3912246636","display_name":"Reprogramming the fate of hematopoietic cells in cyprinid fish","funder_award_id":"GA24-10486S","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"},{"id":"https://openalex.org/G4459401953","display_name":null,"funder_award_id":"LM2023052","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"},{"id":"https://openalex.org/G5907117708","display_name":null,"funder_award_id":"RVO 68378050","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"},{"id":"https://openalex.org/G6242581989","display_name":null,"funder_award_id":"68378050","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"},{"id":"https://openalex.org/G8740927120","display_name":null,"funder_award_id":"FKZ 01KI1826","funder_id":"https://openalex.org/F4320321114","funder_display_name":"Bundesministerium f\u00fcr Bildung und Forschung"}],"funders":[{"id":"https://openalex.org/F4320311614","display_name":"Universit\u00e9 de Sherbrooke","ror":"https://ror.org/00kybxq39"},{"id":"https://openalex.org/F4320314161","display_name":"Joint Programming Initiative on Antimicrobial Resistance","ror":null},{"id":"https://openalex.org/F4320321006","display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","ror":"https://ror.org/01pv73b02"},{"id":"https://openalex.org/F4320321114","display_name":"Bundesministerium f\u00fcr Bildung und Forschung","ror":"https://ror.org/04pz7b180"},{"id":"https://openalex.org/F4320323299","display_name":"Norges Forskningsr\u00e5d","ror":"https://ror.org/00epmv149"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204856617.pdf","grobid_xml":"https://content.openalex.org/works/W7204856617.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2743995608","https://openalex.org/W1994315237","https://openalex.org/W1986609589","https://openalex.org/W7042981147","https://openalex.org/W2428283575","https://openalex.org/W3119808766","https://openalex.org/W7095421124","https://openalex.org/W7030029932","https://openalex.org/W1985405877","https://openalex.org/W4398254927"],"abstract_inverted_index":{"Structured":[0],"regulatory":[1],"RNAs,":[2],"such":[3,24,75],"as":[4,25,76,93,158],"riboswitches,":[5],"represent":[6],"attractive":[7],"yet":[8],"challenging":[9],"targets":[10],"for":[11,145],"small\u2011molecule":[12],"discovery.":[13],"Many":[14],"existing":[15],"screening":[16,35],"approaches":[17],"prioritize":[18],"ligand":[19],"binding":[20],"over":[21],"their":[22],"function,":[23],"controlling":[26],"gene":[27],"expression.":[28],"Here,":[29],"we":[30,96],"describe":[31],"an":[32],"activity\u2011based":[33],"high\u2011throughput":[34],"platform":[36,99,157],"designed":[37],"to":[38,65],"identify":[39],"effector":[40,178],"compounds":[41],"of":[42,118,142,155,167,177,180,187,191],"off\u2011type":[43],"riboswitches":[44,168],"by":[45],"directly":[46],"reporting":[47],"transcriptional":[48,77,173],"readthrough.":[49],"The":[50],"assay":[51,107,131],"combines":[52],"a":[53,60,83,94,101,113,159],"miniaturized":[54,114],"in":[55,103,112,196],"vitro":[56,104],"transcription":[57,105],"system":[58],"with":[59,139],"dual":[61],"molecular":[62],"beacon":[63],"readout":[64],"enable":[66],"discrimination":[67],"between":[68],"ligand\u2011induced":[69],"riboswitch":[70,92],"control":[71],"and":[72,108,133,184],"non\u2011specific":[73],"effects":[74],"inhibition":[78],"or":[79],"fluorescence":[80],"interference.":[81],"Using":[82],"previously":[84],"uncharacterized":[85],"Staphylococcus":[86],"aureus":[87],"thiD":[88],"thiamine":[89],"pyrophosphate":[90],"(TPP)":[91],"model,":[95],"validate":[97],"the":[98,130,134,146,153,156,165,172,175,185,188],"against":[100],"radiolabelled":[102],"readthrough":[106],"demonstrate":[109],"robust":[110],"performance":[111],"1536-well":[115],"format.":[116],"Screening":[117],"several":[119],"diverse":[120],"compound":[121],"libraries":[122],"did":[123],"not":[124],"yield":[125],"validated":[126],"non\u2011native":[127],"modulators":[128],"under":[129],"conditions":[132],"chemical":[135],"space":[136],"explored,":[137],"consistent":[138],"previous":[140],"predictions":[141],"low":[143],"druggability":[144],"TPP":[147],"riboswitch.":[148],"Importantly,":[149],"these":[150],"results":[151],"highlight":[152],"utility":[154],"stringent":[160],"functional":[161,189],"filter":[162],"that":[163,169],"enables":[164],"identification":[166,176],"operate":[170],"at":[171],"level,":[174],"ligands":[179],"bacterial":[181],"RNA":[182,192],"targets,":[183,193],"assessment":[186],"ligandability":[190],"thereby":[194],"aiding":[195],"target":[197],"prioritization.":[198]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-01T00:00:00"}