{"id":"https://openalex.org/W7204827170","doi":"https://doi.org/10.26434/chemrxiv.15008046/v1","title":"Biochemical Investigation of the Role of Loop 1 Residues in LytM","display_name":"Biochemical Investigation of the Role of Loop 1 Residues in LytM","publication_year":2026,"publication_date":"2026-08-30","ids":{"openalex":"https://openalex.org/W7204827170","doi":"https://doi.org/10.26434/chemrxiv.15008046/v1"},"language":"en","primary_location":{"id":"doi:10.26434/chemrxiv.15008046/v1","doi":"https://doi.org/10.26434/chemrxiv.15008046/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008046/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008046/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008046/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5148810582","display_name":"Sophia Y. Gonzalez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]},{"id":"https://openalex.org/I872248612","display_name":"Claremont Colleges","ror":"https://ror.org/03xaz7s88","country_code":"US","type":"education","lineage":["https://openalex.org/I872248612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sophia Y. Gonzalez","raw_affiliation_strings":["Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA","institution_ids":["https://openalex.org/I16970960","https://openalex.org/I872248612"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148781901","display_name":"Cassidy A. McIntyre","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]},{"id":"https://openalex.org/I872248612","display_name":"Claremont Colleges","ror":"https://ror.org/03xaz7s88","country_code":"US","type":"education","lineage":["https://openalex.org/I872248612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cassidy A. McIntyre","raw_affiliation_strings":["Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA","institution_ids":["https://openalex.org/I16970960","https://openalex.org/I872248612"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148822047","display_name":"Amber L. B. Mogg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]},{"id":"https://openalex.org/I872248612","display_name":"Claremont Colleges","ror":"https://ror.org/03xaz7s88","country_code":"US","type":"education","lineage":["https://openalex.org/I872248612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amber L. B. Mogg","raw_affiliation_strings":["Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA","institution_ids":["https://openalex.org/I16970960","https://openalex.org/I872248612"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148765872","display_name":"Manya P. Sharma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]},{"id":"https://openalex.org/I872248612","display_name":"Claremont Colleges","ror":"https://ror.org/03xaz7s88","country_code":"US","type":"education","lineage":["https://openalex.org/I872248612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Manya P. Sharma","raw_affiliation_strings":["Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA","institution_ids":["https://openalex.org/I16970960","https://openalex.org/I872248612"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026700487","display_name":"Sierra J. Williams","orcid":"https://orcid.org/0000-0002-0519-8357","observed_orcids":["https://orcid.org/0000-0002-0519-8357"]},"institutions":[{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]},{"id":"https://openalex.org/I872248612","display_name":"Claremont Colleges","ror":"https://ror.org/03xaz7s88","country_code":"US","type":"education","lineage":["https://openalex.org/I872248612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sierra J. Williams","raw_affiliation_strings":["Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA"],"raw_orcid":"https://orcid.org/0000-0002-0519-8357","affiliations":[{"raw_affiliation_string":"Department of Natural Sciences of Pitzer and Scripps Colleges The Claremont Colleges 925 N. Mills Ave. , Claremont , CA 91711 ( USA","institution_ids":["https://openalex.org/I16970960","https://openalex.org/I872248612"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.4309000074863434,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.4309000074863434,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.1316000074148178,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.12950000166893005,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lytm","display_name":"LytM","score":0.9980000257492065},{"id":"https://openalex.org/keywords/peptidoglycan-hydrolase","display_name":"peptidoglycan hydrolase","score":0.8820000290870667},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.8600000143051147},{"id":"https://openalex.org/keywords/cleavage-site","display_name":"cleavage site","score":0.7020000219345093},{"id":"https://openalex.org/keywords/structure-function-relationship","display_name":"structure-function relationship","score":0.6320000290870667},{"id":"https://openalex.org/keywords/alanine-scanning","display_name":"alanine scanning","score":0.7239999771118164},{"id":"https://openalex.org/keywords/saturation-mutagenesis","display_name":"saturation mutagenesis","score":0.949999988079071},{"id":"https://openalex.org/keywords/lysostaphin","display_name":"lysostaphin","score":0.9900000095367432},{"id":"https://openalex.org/keywords/drug-resistant-bacteria","display_name":"drug-resistant bacteria","score":0.6380000114440918},{"id":"https://openalex.org/keywords/antimicrobial-enzymes","display_name":"antimicrobial enzymes","score":0.5080000162124634},{"id":"https://openalex.org/keywords/catalytic-activity","display_name":"catalytic activity","score":0.5690000057220459}],"concepts":[{"id":"https://openalex.org/C2776809874","wikidata":"https://www.wikidata.org/wiki/Q4826378","display_name":"Autolysin","level":4,"score":0.7405999898910522},{"id":"https://openalex.org/C2776634448","wikidata":"https://www.wikidata.org/wiki/Q206920","display_name":"Peptidoglycan","level":3,"score":0.545199990272522},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.508899986743927},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4805000126361847},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46720001101493835},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.46470001339912415},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4507000148296356},{"id":"https://openalex.org/C2780227090","wikidata":"https://www.wikidata.org/wiki/Q2636892","display_name":"Alanine scanning","level":5,"score":0.43560001254081726},{"id":"https://openalex.org/C2779856020","wikidata":"https://www.wikidata.org/wiki/Q218642","display_name":"Alanine","level":3,"score":0.4138999879360199},{"id":"https://openalex.org/C184670325","wikidata":"https://www.wikidata.org/wiki/Q512604","display_name":"Loop (graph theory)","level":2,"score":0.4011000096797943},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.38850000500679016},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.37139999866485596},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3540000021457672},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.3336000144481659},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3253999948501587},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.30559998750686646},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3001999855041504},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.29499998688697815},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.29010000824928284},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2660999894142151},{"id":"https://openalex.org/C2780120296","wikidata":"https://www.wikidata.org/wiki/Q96286","display_name":"Hydrolase","level":3,"score":0.26429998874664307},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.2630999982357025}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.26434/chemrxiv.15008046/v1","doi":"https://doi.org/10.26434/chemrxiv.15008046/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008046/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008046/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.26434/chemrxiv.15008046/v1","doi":"https://doi.org/10.26434/chemrxiv.15008046/v1","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv.15008046/v1","pdf_url":"https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15008046/v1","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204827170.pdf","grobid_xml":"https://content.openalex.org/works/W7204827170.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4404026337","https://openalex.org/W4388230835","https://openalex.org/W4386998904","https://openalex.org/W2410786744","https://openalex.org/W4412615203","https://openalex.org/W4377826692","https://openalex.org/W4248977248","https://openalex.org/W7112438761","https://openalex.org/W2141249507","https://openalex.org/W2037964985"],"abstract_inverted_index":{"Infections":[0],"caused":[1],"by":[2,49],"drug-resistant":[3],"bacteria":[4],"impact":[5],"the":[6,11,125,130,144],"lives":[7],"of":[8,35,56,124,129,147,152,180],"millions":[9],"across":[10],"globe.":[12],"To":[13],"combat":[14],"these":[15,30,148,153],"infections,":[16],"peptidoglycan":[17],"hydrolases":[18],"(PGHs)":[19],"are":[20,100,176],"gaining":[21],"interest":[22],"due":[23],"to":[24,27,74,113,142,163],"their":[25],"ability":[26],"effectively":[28],"kill":[29],"pathogens":[31],"with":[32],"low":[33],"rates":[34],"evoking":[36],"resistance":[37],"pathways.":[38],"One":[39],"such":[40],"PGH":[41],"includes":[42],"LytM,":[43],"which":[44],"is":[45,65,93,168],"an":[46,53],"autolysin":[47],"produced":[48],"Staphylococcus":[50],"aureus.":[51],"While":[52],"engineered":[54],"variant":[55],"this":[57,106,171],"enzyme":[58,131],"has":[59,83],"shown":[60],"antimicrobial":[61],"activity,":[62],"its":[63],"mechanism":[64],"poorly":[66],"understood.":[67],"The":[68,150],"loop":[69,98,126],"1":[70,99,127],"region":[71,128],"was":[72,111,155],"proposed":[73],"be":[75],"responsible":[76],"for":[77,87,102,119,135,178],"a":[78,108,159],"new":[79],"cleavage":[80],"site":[81],"that":[82,116,175],"not":[84],"been":[85],"reported":[86],"other":[88],"M23":[89],"enzymes.":[90,184],"However,":[91],"it":[92],"unclear":[94],"what":[95],"residues":[96,154,174],"in":[97,158],"key":[101,133],"catalytic":[103],"activity.":[104,120],"In":[105],"work,":[107],"biochemical":[109],"analysis":[110],"used":[112,139],"identify":[114,143],"sites":[115],"were":[117],"important":[118,177],"An":[121],"alanine":[122],"scan":[123],"revealed":[132],"hotspots":[134],"hydrolysis.":[136],"We":[137],"then":[138,156],"site-saturation":[140],"mutagenesis":[141],"structure-function":[145],"relationship":[146],"sites.":[149],"function":[151],"tested":[157],"homologous":[160],"enzyme,":[161],"lysostaphin,":[162],"determine":[164],"how":[165],"well":[166],"activity":[167,179],"conserved.":[169],"Collectively,":[170],"work":[172],"confirms":[173],"LytM":[181],"and":[182],"related":[183]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-01T00:00:00"}