{"id":"https://openalex.org/W7204813768","doi":"https://doi.org/10.64898/2026.08.29.741951","title":"High-Molecular-Weight Genomic DNA Extraction from Recalcitrant Australian Plants: An Optimised CTAB Protocol for Anigozanthos","display_name":"High-Molecular-Weight Genomic DNA Extraction from Recalcitrant Australian Plants: An Optimised CTAB Protocol for Anigozanthos","publication_year":2026,"publication_date":"2026-08-31","ids":{"openalex":"https://openalex.org/W7204813768","doi":"https://doi.org/10.64898/2026.08.29.741951"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.29.741951","doi":"https://doi.org/10.64898/2026.08.29.741951","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.741951","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/31/2026.08.29.741951.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/31/2026.08.29.741951.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5144120278","display_name":"Rashika Rajput","orcid":"https://orcid.org/0000-0002-7665-5973","observed_orcids":["https://orcid.org/0000-0002-7665-5973"]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I4210102918","display_name":"ARC Centre of Excellence in Plant Energy Biology","ror":"https://ror.org/01a1mq059","country_code":"AU","type":"facility","lineage":["https://openalex.org/I118347636","https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210102918","https://openalex.org/I4210132349"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Rashika Rajput","raw_affiliation_strings":["ARC Centre of Excellence in Plant Energy Biology, Australia","School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":"https://orcid.org/0000-0002-7665-5973","affiliations":[{"raw_affiliation_string":"ARC Centre of Excellence in Plant Energy Biology, Australia","institution_ids":["https://openalex.org/I4210102918"]},{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036367473","display_name":"Linkon Saha","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Linkon Saha","raw_affiliation_strings":["School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148805936","display_name":"Zeeshan Ahmed","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I4210102918","display_name":"ARC Centre of Excellence in Plant Energy Biology","ror":"https://ror.org/01a1mq059","country_code":"AU","type":"facility","lineage":["https://openalex.org/I118347636","https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210102918","https://openalex.org/I4210132349"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Zeeshan Ahmed","raw_affiliation_strings":["ARC Centre of Excellence in Plant Energy Biology, Australia","School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ARC Centre of Excellence in Plant Energy Biology, Australia","institution_ids":["https://openalex.org/I4210102918"]},{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148821463","display_name":"Pia Naiker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Pia Naiker","raw_affiliation_strings":["School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014168939","display_name":"Lien Do","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Lien Do","raw_affiliation_strings":["School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148792911","display_name":"Andrew Bisset","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Andrew Bisset","raw_affiliation_strings":["School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002166404","display_name":"Cornelia M. Hooper","orcid":"https://orcid.org/0000-0002-3471-8286","observed_orcids":["https://orcid.org/0000-0002-3471-8286"]},"institutions":[{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I4210102918","display_name":"ARC Centre of Excellence in Plant Energy Biology","ror":"https://ror.org/01a1mq059","country_code":"AU","type":"facility","lineage":["https://openalex.org/I118347636","https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210102918","https://openalex.org/I4210132349"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Cornelia M Hooper","raw_affiliation_strings":["ARC Centre of Excellence in Plant Energy Biology, Australia","School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia"],"raw_orcid":"https://orcid.org/0000-0002-3471-8286","affiliations":[{"raw_affiliation_string":"ARC Centre of Excellence in Plant Energy Biology, Australia","institution_ids":["https://openalex.org/I4210102918"]},{"raw_affiliation_string":"School of Molecular Sciences, The University of Western Australia, Crawley WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5002166404"],"corresponding_institution_ids":["https://openalex.org/I177877127","https://openalex.org/I4210102918"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.37869998812675476,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.37869998812675476,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.23100000619888306,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.18240000307559967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genomic-dna-extraction","display_name":"genomic DNA extraction","score":0.8709999918937683},{"id":"https://openalex.org/keywords/recalcitrant-plants","display_name":"recalcitrant plants","score":0.8629999756813049},{"id":"https://openalex.org/keywords/anigozanthos","display_name":"Anigozanthos","score":0.9049999713897705},{"id":"https://openalex.org/keywords/haemodoraceae","display_name":"Haemodoraceae","score":0.9150000214576721},{"id":"https://openalex.org/keywords/dna-fragment-size","display_name":"DNA fragment size","score":0.5090000033378601},{"id":"https://openalex.org/keywords/plant-genomics","display_name":"plant genomics","score":0.5929999947547913}],"concepts":[{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6570000052452087},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6075999736785889},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.5677000284194946},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5230000019073486},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.46810001134872437},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.45210000872612},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.40720000863075256},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.3917999863624573},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.38589999079704285},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.3799999952316284},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.3772999942302704},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.3531999886035919},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3499000072479248},{"id":"https://openalex.org/C157369684","wikidata":"https://www.wikidata.org/wiki/Q34740","display_name":"Genus","level":2,"score":0.3230000138282776},{"id":"https://openalex.org/C2778576202","wikidata":"https://www.wikidata.org/wiki/Q2540164","display_name":"Sample preparation","level":2,"score":0.2924000024795532},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2879999876022339},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.27889999747276306},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.27149999141693115},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.267300009727478},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.25360000133514404},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.2513999938964844}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.29.741951","doi":"https://doi.org/10.64898/2026.08.29.741951","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.741951","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/31/2026.08.29.741951.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.29.741951","doi":"https://doi.org/10.64898/2026.08.29.741951","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.741951","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/31/2026.08.29.741951.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5617149545","display_name":"Unravelling the genetics of Kangaroo paws for climate-resilient gardens","funder_award_id":"IE230100040","funder_id":"https://openalex.org/F4320334704","funder_display_name":"Australian Research Council"}],"funders":[{"id":"https://openalex.org/F4320320591","display_name":"Macquarie University","ror":"https://ror.org/01sf06y89"},{"id":"https://openalex.org/F4320334704","display_name":"Australian Research Council","ror":"https://ror.org/05mmh0f86"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204813768.pdf","grobid_xml":"https://content.openalex.org/works/W7204813768.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W7171948387"],"related_works":["https://openalex.org/W7171948387","https://openalex.org/W2098504913","https://openalex.org/W4388440449","https://openalex.org/W7099027292","https://openalex.org/W2990554466","https://openalex.org/W1972295543","https://openalex.org/W2357836264","https://openalex.org/W2150244850","https://openalex.org/W2062137247","https://openalex.org/W1512893145"],"abstract_inverted_index":{"Abstract":[0],"High-phenolic":[1],"plant":[2],"genera":[3,113],"present":[4],"a":[5,78,101,120,149,159],"major":[6],"technical":[7],"limitation":[8],"in":[9,59,82,129],"genomic":[10,153,163],"research.":[11],"Standard":[12],"extraction":[13,57],"approaches":[14],"that":[15,125,157],"perform":[16,22],"reliably":[17],"across":[18,100,110,165],"diverse":[19,102,112],"flora":[20],"often":[21],"poorly":[23],"when":[24],"applied":[25],"to":[26,68,152],"recalcitrant":[27],"taxa,":[28],"producing":[29],"low":[30],"DNA":[31,83,95],"yield":[32],"and":[33,62,71,90,107],"integrity":[34],"incompatible":[35],"with":[36],"sequencing":[37],"requirements.":[38],"The":[39,74],"genus":[40,61],"Anigozanthos":[41,156],"(Kangaroo":[42],"paws)":[43],"from":[44],"the":[45,140],"family":[46],"Haemodoraceae":[47,115],"exemplifies":[48],"this":[49,60],"problem.":[50],"We":[51,117],"identified":[52],"key":[53],"physicochemical":[54],"factors":[55],"governing":[56],"failure":[58],"resolved":[63],"them":[64],"through":[65],"targeted":[66],"modifications":[67],"lysis":[69],"chemistry":[70],"contaminant":[72,87],"management.":[73],"resulting":[75],"protocol":[76],"achieved":[77],"near":[79],"threefold":[80],"improvement":[81],"purity,":[84],"substantially":[85],"reducing":[86],"carry":[88],"over":[89],"consistently":[91],"yielded":[92],"high-integrity,":[93],"long":[94],"fragments":[96],"(DIN":[97],">":[98],"7)":[99],"sample":[103],"set":[104],"spanning":[105],"cultivated":[106],"wild":[108],"material":[109],"four":[111],"of":[114,155],".":[116],"also":[118],"tested":[119],"straightforward":[121],"purity":[122],"assessment":[123,138],"framework":[124],"can":[126],"be":[127],"implemented":[128],"any":[130],"standard":[131],"molecular":[132],"laboratory,":[133],"enabling":[134],"rapid":[135],"pre-submission":[136],"quality":[137],"without":[139],"need":[141],"for":[142,162],"specialised":[143],"equipment.":[144],"Together":[145],"these":[146],"advances":[147],"open":[148],"practical":[150],"path":[151],"characterisation":[154],"establishes":[158],"transferable":[160],"model":[161],"research":[164],"Australia\u2019s":[166],"chemically":[167],"complex":[168],"native":[169],"flora.":[170]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-09-01T00:00:00"}
