{"id":"https://openalex.org/W7204787632","doi":"https://doi.org/10.23857/pc.v11i7.12199","title":"Plataformas de producci\u00f3n de prote\u00ednas recombinantes libres de antibi\u00f3ticos e IPTG: autoinducci\u00f3n basada en el regul\u00f3n pho y sistemas sint\u00e9ticos de selecci\u00f3n plasm\u00eddica para aplicaciones industriales","display_name":"Plataformas de producci\u00f3n de prote\u00ednas recombinantes libres de antibi\u00f3ticos e IPTG: autoinducci\u00f3n basada en el regul\u00f3n pho y sistemas sint\u00e9ticos de selecci\u00f3n plasm\u00eddica para aplicaciones industriales","publication_year":2026,"publication_date":"2026-07-01","ids":{"openalex":"https://openalex.org/W7204787632","doi":"https://doi.org/10.23857/pc.v11i7.12199"},"language":"es","primary_location":{"id":"doi:10.23857/pc.v11i7.12199","doi":"https://doi.org/10.23857/pc.v11i7.12199","is_oa":true,"landing_page_url":"https://doi.org/10.23857/pc.v11i7.12199","pdf_url":"https://polodelconocimiento.com/ojs/index.php/es/article/download/12199/30989","source":{"id":"https://openalex.org/S4210240955","display_name":"Polo del Conocimiento","issn_l":"2550-682X","issn":["2550-682X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["erih-plus"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Polo del Conocimiento","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://polodelconocimiento.com/ojs/index.php/es/article/download/12199/30989","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5148818495","display_name":"Shirley Janyna Calder\u00f3n Mart\u00ednez","orcid":"https://orcid.org/0000-0002-8039-2897","observed_orcids":["https://orcid.org/0000-0002-8039-2897"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shirley Janyna Calder\u00f3n Mart\u00ednez","raw_affiliation_strings":["Centro de Biociencias Clonallyx Corporation. Quito, Ecuador"],"raw_orcid":"https://orcid.org/0000-0002-8039-2897","affiliations":[{"raw_affiliation_string":"Centro de Biociencias Clonallyx Corporation. Quito, Ecuador","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148786983","display_name":"Britanny Noem\u00ed Gavilanes Quimbita","orcid":"https://orcid.org/0000-0003-2376-0558","observed_orcids":["https://orcid.org/0000-0003-2376-0558"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Britanny Noem\u00ed Gavilanes Quimbita","raw_affiliation_strings":["Centro de Biociencias Clonallyx Corporation. Quito, Ecuador"],"raw_orcid":"https://orcid.org/0000-0003-2376-0558","affiliations":[{"raw_affiliation_string":"Centro de Biociencias Clonallyx Corporation. Quito, Ecuador","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5098981661","display_name":"Samantha Cecibel Guzm\u00e1n Pucha","orcid":"https://orcid.org/0000-0002-8063-9802","observed_orcids":["https://orcid.org/0000-0002-8063-9802"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Samantha Cecibel Guzm\u00e1n Pucha","raw_affiliation_strings":["Centro de Biociencias Clonallyx Corporation. Quito, Ecuador"],"raw_orcid":"https://orcid.org/0000-0002-8063-9802","affiliations":[{"raw_affiliation_string":"Centro de Biociencias Clonallyx Corporation. Quito, Ecuador","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045441695","display_name":"Cesar David Guerra Naranjo","orcid":"https://orcid.org/0000-0001-6651-9591","observed_orcids":["https://orcid.org/0000-0001-6651-9591"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C\u00e9sar David Guerra Naranjo","raw_affiliation_strings":["Centro de Biociencias Clonallyx Corporation. Quito, Ecuador"],"raw_orcid":"https://orcid.org/0000-0001-6651-9591","affiliations":[{"raw_affiliation_string":"Centro de Biociencias Clonallyx Corporation. Quito, Ecuador","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"11","issue":"7","first_page":"3340","last_page":"3364"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.5185999870300293,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.5185999870300293,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.459199994802475,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9459999799728394},{"id":"https://openalex.org/keywords/antibiotic-free-production","display_name":"antibiotic-free production","score":0.6959999799728394},{"id":"https://openalex.org/keywords/pho-regulon","display_name":"Pho regulon","score":0.8259999752044678},{"id":"https://openalex.org/keywords/phosphate-starvation","display_name":"phosphate limitation","score":0.6470000147819519},{"id":"https://openalex.org/keywords/autoinduction","display_name":"autoinduction","score":0.7009999752044678},{"id":"https://openalex.org/keywords/plasmid-selection","display_name":"plasmid selection","score":0.6769999861717224},{"id":"https://openalex.org/keywords/toxin-antitoxin-systems","display_name":"toxin-antitoxin systems","score":0.9470000267028809},{"id":"https://openalex.org/keywords/alternative-carbon-sources","display_name":"alternative carbon sources","score":0.8629999756813049},{"id":"https://openalex.org/keywords/cryptic-plasmid","display_name":"cryptic plasmid","score":0.9869999885559082},{"id":"https://openalex.org/keywords/chromosomal-integration","display_name":"chromosomal integration","score":0.9309999942779541},{"id":"https://openalex.org/keywords/genetic-stability","display_name":"genetic stability","score":0.8709999918937683},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.640999972820282}],"concepts":[{"id":"https://openalex.org/C15708023","wikidata":"https://www.wikidata.org/wiki/Q80083","display_name":"Humanities","level":1,"score":0.46959999203681946},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.40149998664855957},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40049999952316284},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2736999988555908},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.18719999492168427},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.16179999709129333},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.13420000672340393},{"id":"https://openalex.org/C3020366717","wikidata":"https://www.wikidata.org/wiki/Q14075","display_name":"Free access","level":2,"score":0.12309999763965607},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11559999734163284},{"id":"https://openalex.org/C3020792226","wikidata":"https://www.wikidata.org/wiki/Q1331083","display_name":"Human use","level":2,"score":0.0997999981045723}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.23857/pc.v11i7.12199","doi":"https://doi.org/10.23857/pc.v11i7.12199","is_oa":true,"landing_page_url":"https://doi.org/10.23857/pc.v11i7.12199","pdf_url":"https://polodelconocimiento.com/ojs/index.php/es/article/download/12199/30989","source":{"id":"https://openalex.org/S4210240955","display_name":"Polo del Conocimiento","issn_l":"2550-682X","issn":["2550-682X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["erih-plus"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Polo del Conocimiento","raw_type":"journal-article"},{"id":"pmh:oai:ojs.polodelconocimiento.com:article/12199","doi":null,"is_oa":true,"landing_page_url":"https://polodelconocimiento.com/ojs/index.php/es/article/view/12199","pdf_url":null,"source":{"id":"https://openalex.org/S4210240955","display_name":"Polo del Conocimiento","issn_l":"2550-682X","issn":["2550-682X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["erih-plus"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"10.23857/pc.v11i7","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.23857/pc.v11i7.12199","doi":"https://doi.org/10.23857/pc.v11i7.12199","is_oa":true,"landing_page_url":"https://doi.org/10.23857/pc.v11i7.12199","pdf_url":"https://polodelconocimiento.com/ojs/index.php/es/article/download/12199/30989","source":{"id":"https://openalex.org/S4210240955","display_name":"Polo del Conocimiento","issn_l":"2550-682X","issn":["2550-682X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["erih-plus"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Polo del Conocimiento","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204787632.pdf","grobid_xml":"https://content.openalex.org/works/W7204787632.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1520777707","https://openalex.org/W2057140623","https://openalex.org/W2463823521","https://openalex.org/W2513042356","https://openalex.org/W2559495878","https://openalex.org/W2562259337","https://openalex.org/W2738984803","https://openalex.org/W2759192910","https://openalex.org/W2806996953","https://openalex.org/W2884822279","https://openalex.org/W2951096514","https://openalex.org/W2952116753","https://openalex.org/W2994949405","https://openalex.org/W3026425396","https://openalex.org/W3083154614","https://openalex.org/W4200523890","https://openalex.org/W4223577114","https://openalex.org/W4281714844","https://openalex.org/W4281931853","https://openalex.org/W4290831365","https://openalex.org/W4296036875","https://openalex.org/W4380183371","https://openalex.org/W4387576434","https://openalex.org/W4390746061","https://openalex.org/W4391234207","https://openalex.org/W4415547469","https://openalex.org/W4417323380","https://openalex.org/W7117102615","https://openalex.org/W7134939326","https://openalex.org/W7162090372"],"related_works":["https://openalex.org/W4281931853","https://openalex.org/W7204675942","https://openalex.org/W641479260","https://openalex.org/W7046587204","https://openalex.org/W2759192910","https://openalex.org/W7110593906","https://openalex.org/W4413810450","https://openalex.org/W89327688","https://openalex.org/W7037329039","https://openalex.org/W2094183738"],"abstract_inverted_index":{"La":[0,177],"producci\u00f3n":[1,54],"de":[2,13,20,53,56,72,82,91,99,104,110,129,140,174,188,211,227,230],"prote\u00ednas":[3,239],"recombinantes":[4],"mediante":[5,84],"sistemas":[6,52,128],"microbianos":[7],"contin\u00faa":[8],"dependiendo,":[9],"en":[10,51,62,69,132,202,221,241],"numerosas":[11],"plataformas,":[12],"antibi\u00f3ticos":[14,57,212],"para":[15,24],"mantener":[16],"los":[17,127],"pl\u00e1smidos":[18,142],"y":[19,41,68,90,106,116,124,171,206,240],"isopropil":[21],"\u03b2-D-1-tiogalactopiran\u00f3sido":[22],"(IPTG)":[23],"inducir":[25],"la":[26,63,79,85,148,161,165,168,195,199,207],"expresi\u00f3n,":[27],"lo":[28],"que":[29,78,198],"genera":[30],"limitaciones":[31],"relacionadas":[32],"con":[33,60,119,237],"carga":[34,166],"metab\u00f3lica,":[35,167],"estabilidad":[36,149,182],"gen\u00e9tica,":[37],"costos":[38],"operativos,":[39],"escalabilidad":[40],"bioseguridad.":[42],"Esta":[43],"revisi\u00f3n":[44],"integrativa":[45],"analiz\u00f3":[46],"art\u00edculos":[47],"cient\u00edficos":[48],"recientes,":[49],"centrados":[50],"libres":[55],"e":[58,144,232],"IPTG,":[59,233],"\u00e9nfasis":[61],"autoinducci\u00f3n":[64,200],"regulada":[65],"por":[66,160],"fosfato":[67],"estrategias":[70],"sint\u00e9ticas":[71],"mantenimiento":[73],"gen\u00e9tico.":[74],"Los":[75],"estudios":[76],"mostraron":[77],"depleci\u00f3n":[80],"controlada":[81],"fosfato,":[83],"activaci\u00f3n":[86],"del":[87,94,163],"sistema":[88],"PhoR\u2013PhoB":[89],"promotores":[92],"dependientes":[93],"regul\u00f3n":[95,204],"Pho,":[96],"permite":[97],"separar":[98],"forma":[100],"aut\u00f3noma":[101],"las":[102],"fases":[103],"crecimiento":[105],"producci\u00f3n,":[107],"alcanzando":[108],"t\u00edtulos":[109],"hasta":[111],"8,1":[112],"\u00b1":[113],"0,7":[114],"g/L":[115],"demostrando":[117],"compatibilidad":[118,169],"cultivos":[120],"fed-batch,":[121],"fed-batch":[122],"repetitivo":[123],"quimiostato.":[125],"Paralelamente,":[126],"selecci\u00f3n":[130,208],"basados":[131],"auxotrof\u00edas,":[133],"genes":[134,229],"esenciales,":[135],"m\u00f3dulos":[136,214],"toxina\u2013antitoxina,":[137],"fuentes":[138],"alternativas":[139],"carbono,":[141],"cr\u00edpticos":[143],"integraci\u00f3n":[145,178,220],"cromos\u00f3mica":[146,179],"mejoraron":[147],"gen\u00e9tica":[150],"sin":[151],"presi\u00f3n":[152],"antibi\u00f3tica.":[153],"No":[154],"obstante,":[155],"su":[156,219],"eficiencia":[157],"estuvo":[158],"condicionada":[159],"expresi\u00f3n":[162],"marcador,":[164],"hospedador\u2013vector":[170],"el":[172,203],"n\u00famero":[173],"copias":[175],"g\u00e9nicas.":[176],"proporcion\u00f3":[180],"una":[181,189,222],"superior,":[183],"aunque":[184],"frecuentemente":[185],"a":[186],"costa":[187],"menor":[190],"dosis":[191],"g\u00e9nica.":[192],"En":[193],"conjunto,":[194],"evidencia":[196],"indica":[197],"basada":[201],"Pho":[205],"sint\u00e9tica":[209],"libre":[210,226],"constituyen":[213],"funcionalmente":[215],"complementarios.":[216],"Sin":[217],"embargo,":[218],"plataforma":[223],"\u00fanica,":[224],"completamente":[225],"antibi\u00f3ticos,":[228],"resistencia":[231],"todav\u00eda":[234],"requiere":[235],"validaci\u00f3n":[236],"m\u00faltiples":[238],"procesos":[242],"industriales":[243],"prolongados.":[244]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-09-01T00:00:00"}