{"id":"https://openalex.org/W7204755985","doi":"https://doi.org/10.64898/2026.08.29.748002","title":"Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin","display_name":"Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin","publication_year":2026,"publication_date":"2026-08-31","ids":{"openalex":"https://openalex.org/W7204755985","doi":"https://doi.org/10.64898/2026.08.29.748002"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.29.748002","doi":"https://doi.org/10.64898/2026.08.29.748002","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.748002","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.08.29.748002","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015341208","display_name":"Simona Marincean","orcid":"https://orcid.org/0000-0003-1393-4868","observed_orcids":["https://orcid.org/0000-0003-1393-4868"]},"institutions":[{"id":"https://openalex.org/I4210130704","display_name":"University of Michigan\u2013Dearborn","ror":"https://ror.org/035wtm547","country_code":"US","type":"education","lineage":["https://openalex.org/I4210130704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Simona Marincean","raw_affiliation_strings":["Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A"],"raw_orcid":"https://orcid.org/0000-0003-1393-4868","affiliations":[{"raw_affiliation_string":"Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A","institution_ids":["https://openalex.org/I4210130704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007886112","display_name":"Sheila Rose Smith","orcid":"https://orcid.org/0000-0003-3805-981X","observed_orcids":["https://orcid.org/0000-0003-3805-981X"]},"institutions":[{"id":"https://openalex.org/I4210130704","display_name":"University of Michigan\u2013Dearborn","ror":"https://ror.org/035wtm547","country_code":"US","type":"education","lineage":["https://openalex.org/I4210130704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sheila R. Smith","raw_affiliation_strings":["Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A"],"raw_orcid":"https://orcid.org/0000-0003-3805-981X","affiliations":[{"raw_affiliation_string":"Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A","institution_ids":["https://openalex.org/I4210130704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094242951","display_name":"Travis Branscum","orcid":"https://orcid.org/0009-0003-6160-0974","observed_orcids":["https://orcid.org/0009-0003-6160-0974"]},"institutions":[{"id":"https://openalex.org/I4210130704","display_name":"University of Michigan\u2013Dearborn","ror":"https://ror.org/035wtm547","country_code":"US","type":"education","lineage":["https://openalex.org/I4210130704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Travis Branscum","raw_affiliation_strings":["Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A"],"raw_orcid":"https://orcid.org/0009-0003-6160-0974","affiliations":[{"raw_affiliation_string":"Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A","institution_ids":["https://openalex.org/I4210130704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018034522","display_name":"Amanda M. Ratajczak","orcid":"https://orcid.org/0000-0002-0796-8044","observed_orcids":["https://orcid.org/0000-0002-0796-8044"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Amanda Ratajczak","raw_affiliation_strings":["Mainline Scientific, 267 Great Valley Parkway, Malvern, Pennsylvania 19355, U.S.A"],"raw_orcid":"https://orcid.org/0000-0002-0796-8044","affiliations":[{"raw_affiliation_string":"Mainline Scientific, 267 Great Valley Parkway, Malvern, Pennsylvania 19355, U.S.A","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010965504","display_name":"Marilee Ann Benore","orcid":"https://orcid.org/0000-0001-5814-4910","observed_orcids":["https://orcid.org/0000-0001-5814-4910"]},"institutions":[{"id":"https://openalex.org/I4210130704","display_name":"University of Michigan\u2013Dearborn","ror":"https://ror.org/035wtm547","country_code":"US","type":"education","lineage":["https://openalex.org/I4210130704"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Marilee Benore","raw_affiliation_strings":["Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A"],"raw_orcid":"https://orcid.org/0000-0001-5814-4910","affiliations":[{"raw_affiliation_string":"Department of Natural Sciences, University of Michigan-Dearborn, 4900 Evergreen Rd., Dearborn, Michigan 48128, U.S.A","institution_ids":["https://openalex.org/I4210130704"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5010965504"],"corresponding_institution_ids":["https://openalex.org/I4210130704"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.7163000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.7163000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.2078000009059906,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.01600000075995922,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/biotin-conjugates","display_name":"biotin conjugates","score":0.7009999752044678},{"id":"https://openalex.org/keywords/riboflavin-binding-protein","display_name":"riboflavin-binding protein","score":0.8740000128746033},{"id":"https://openalex.org/keywords/streptavidin","display_name":"streptavidin","score":0.8659999966621399},{"id":"https://openalex.org/keywords/protein-binding-affinity","display_name":"protein binding affinity","score":0.8809999823570251},{"id":"https://openalex.org/keywords/fluorescence-quenching","display_name":"fluorescence quenching","score":0.8820000290870667},{"id":"https://openalex.org/keywords/flavin-binding-proteins","display_name":"flavin-binding proteins","score":0.652999997138977},{"id":"https://openalex.org/keywords/protein-labeling","display_name":"protein labeling","score":0.6819999814033508}],"concepts":[{"id":"https://openalex.org/C2778204606","wikidata":"https://www.wikidata.org/wiki/Q181354","display_name":"Biotin","level":2,"score":0.7918999791145325},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7495999932289124},{"id":"https://openalex.org/C2776155809","wikidata":"https://www.wikidata.org/wiki/Q130365","display_name":"Riboflavin","level":2,"score":0.7056999802589417},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5551000237464905},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5521000027656555},{"id":"https://openalex.org/C2776190903","wikidata":"https://www.wikidata.org/wiki/Q421817","display_name":"Streptavidin","level":3,"score":0.5436000227928162},{"id":"https://openalex.org/C33093398","wikidata":"https://www.wikidata.org/wiki/Q174337","display_name":"Flavin group","level":3,"score":0.5085999965667725},{"id":"https://openalex.org/C2775859393","wikidata":"https://www.wikidata.org/wiki/Q28746","display_name":"Flavin adenine dinucleotide","level":4,"score":0.49470001459121704},{"id":"https://openalex.org/C2780283098","wikidata":"https://www.wikidata.org/wiki/Q4688960","display_name":"Affinities","level":2,"score":0.49079999327659607},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.4499000012874603},{"id":"https://openalex.org/C2780523633","wikidata":"https://www.wikidata.org/wiki/Q380709","display_name":"Labelling","level":2,"score":0.4350000023841858},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.42089998722076416},{"id":"https://openalex.org/C111771559","wikidata":"https://www.wikidata.org/wiki/Q66295","display_name":"Derivative (finance)","level":2,"score":0.397599995136261},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.37720000743865967},{"id":"https://openalex.org/C3018795828","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Binding affinities","level":3,"score":0.367900013923645},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.365200012922287},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3481999933719635},{"id":"https://openalex.org/C176406525","wikidata":"https://www.wikidata.org/wiki/Q24775166","display_name":"Avidin","level":3,"score":0.32280001044273376},{"id":"https://openalex.org/C96739231","wikidata":"https://www.wikidata.org/wiki/Q4914025","display_name":"Binding selectivity","level":2,"score":0.31209999322891235},{"id":"https://openalex.org/C2780679169","wikidata":"https://www.wikidata.org/wiki/Q4688981","display_name":"Affinity label","level":3,"score":0.3073999881744385},{"id":"https://openalex.org/C71995715","wikidata":"https://www.wikidata.org/wiki/Q78574842","display_name":"Flavoprotein","level":3,"score":0.30239999294281006},{"id":"https://openalex.org/C553089730","wikidata":"https://www.wikidata.org/wiki/Q4914021","display_name":"Binding protein","level":3,"score":0.29840001463890076},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.2847000062465668},{"id":"https://openalex.org/C85196483","wikidata":"https://www.wikidata.org/wiki/Q6545857","display_name":"Ligand binding assay","level":3,"score":0.2759000062942505},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.27390000224113464},{"id":"https://openalex.org/C3020471959","wikidata":"https://www.wikidata.org/wiki/Q183206","display_name":"B vitamins","level":2,"score":0.26589998602867126}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.29.748002","doi":"https://doi.org/10.64898/2026.08.29.748002","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.748002","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.29.748002","doi":"https://doi.org/10.64898/2026.08.29.748002","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.29.748002","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332921","display_name":"University of Michigan-Dearborn","ror":"https://ror.org/035wtm547"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1220810384","https://openalex.org/W1578798626","https://openalex.org/W1754901624","https://openalex.org/W1988774285","https://openalex.org/W1997806799","https://openalex.org/W2008949282","https://openalex.org/W2039485830","https://openalex.org/W2049533699","https://openalex.org/W2091146030","https://openalex.org/W2100837269","https://openalex.org/W2418583649","https://openalex.org/W2438141366","https://openalex.org/W2551065150","https://openalex.org/W2567307082","https://openalex.org/W2745131378","https://openalex.org/W2915722979","https://openalex.org/W2951116953","https://openalex.org/W3004028890","https://openalex.org/W3031970934","https://openalex.org/W4210307959","https://openalex.org/W4387702147","https://openalex.org/W4400726327","https://openalex.org/W4403502230","https://openalex.org/W4406178791","https://openalex.org/W4406189893","https://openalex.org/W4409706900","https://openalex.org/W4411923760","https://openalex.org/W7128628796","https://openalex.org/W7131077782"],"related_works":["https://openalex.org/W4393167839","https://openalex.org/W2605271699","https://openalex.org/W2013638209","https://openalex.org/W2469969151","https://openalex.org/W2039485830","https://openalex.org/W2027857781","https://openalex.org/W4255716631","https://openalex.org/W2000094205","https://openalex.org/W2024745473","https://openalex.org/W1985635124"],"abstract_inverted_index":{"Abstract":[0],"The":[1,84],"binding":[2,50,82,120,129,132,135,152],"affinities":[3,153],"of":[4,8,109,122],"a":[5,9,110],"chimeric":[6,124,141],"analog":[7],"riboflavin":[10,49,111,131],"derivative":[11,112],"linked":[12,113],"to":[13,44,47,91,103,114,130,149],"biotin,":[14],"(":[15],"6-(7":[16],",":[17,19,21,29,32,38,42],"8-dimethyl-2":[18],"4-dioxo-3":[20],"4-dihydrobenzo[":[22],"g":[23],"]pteridin-10(2":[24],"H":[25,36],")-yl)hexyl":[26],"5-((3a":[27],"S":[28,31],"4":[30],"6a":[33],"R":[34],")-2-oxohexahydro-1":[35],"-thieno[3":[37],"4-":[39],"d":[40],"]imidazol-4-yl)pentanoate)":[41],"referred":[43],"as":[45],"C6-Rf-biotin-tag,":[46],"the":[48,56,123,140,151],"retain":[51],"or":[52,137],"streptavidin":[53,138],"are":[54],"in":[55,75],"\u03bcM":[57],"range,":[58],"1.29":[59],"\u00b1":[60,64],"0.277":[61],"and":[62,78,93,99],"3.00":[63],"0.459,":[65],"respectively.":[66],"These":[67],"values":[68],"suggest":[69],"that":[70],"C6-Rf-biotin-tag":[71,85],"has":[72],"potential":[73],"applications":[74],"diagnostic":[76],"assay":[77],"labelling":[79],"target":[80],"flavin":[81],"proteins.":[83],"which":[86],"was":[87,116,126,147],"characterized":[88],"with":[89],"respect":[90],"physical":[92],"biochemical":[94],"properties":[95],"retains":[96],"UV/Vis":[97],"spectroscopic":[98],"fluorescence":[100],"behavior":[101],"similar":[102],"riboflavin.":[104],"Highlights":[105],"A":[106],"novel":[107],"molecule":[108,125,142],"biotin":[115],"tested":[117],"for":[118],"protein":[119,133,136],"Fluorescence":[121],"quenched":[127],"upon":[128],"Riboflavin":[134],"bind":[139],"Plasmon":[143],"waveguide":[144],"resonance":[145],"spectroscopy":[146],"used":[148],"determine":[150]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-09-01T00:00:00"}
