{"id":"https://openalex.org/W7204498441","doi":"https://doi.org/10.3892/etm.2026.13279","title":"Generation and evaluation of multimers of anti C\u2011reactive protein Nb and alkaline phosphatase based on three self\u2011assembly peptides","display_name":"Generation and evaluation of multimers of anti C\u2011reactive protein Nb and alkaline phosphatase based on three self\u2011assembly peptides","publication_year":2026,"publication_date":"2026-08-28","ids":{"openalex":"https://openalex.org/W7204498441","doi":"https://doi.org/10.3892/etm.2026.13279","pmid":"https://pubmed.ncbi.nlm.nih.gov/42713007","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13551084"},"language":"en","primary_location":{"id":"doi:10.3892/etm.2026.13279","doi":"https://doi.org/10.3892/etm.2026.13279","is_oa":true,"landing_page_url":"https://doi.org/10.3892/etm.2026.13279","pdf_url":"http://www.spandidos-publications.com/10.3892/etm.2026.13279/download","source":{"id":"https://openalex.org/S151424340","display_name":"Experimental and Therapeutic Medicine","issn_l":"1792-0981","issn":["1792-0981","1792-1015"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","poland-40","vabb-shw"],"host_organization":"https://openalex.org/P4310320412","host_organization_name":"Spandidos Publishing","host_organization_lineage":["https://openalex.org/P4310320412"],"host_organization_lineage_names":["Spandidos Publishing"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental and Therapeutic Medicine","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"http://www.spandidos-publications.com/10.3892/etm.2026.13279/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090558481","display_name":"Honghe Li","orcid":"https://orcid.org/0000-0002-3333-6147","observed_orcids":["https://orcid.org/0000-0002-3333-6147"]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Honghe Li","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020912053","display_name":"Chenchen Zhou","orcid":"https://orcid.org/0000-0002-6427-5869","observed_orcids":["https://orcid.org/0000-0002-6427-5869"]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chenchen Zhou","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148564734","display_name":"Xiaomei Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xiaomei Zhang","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148552080","display_name":"Ning Ding","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ning Ding","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100638546","display_name":"Qiong Wu","orcid":"https://orcid.org/0000-0003-3305-6652","observed_orcids":["https://orcid.org/0000-0003-3305-6652","https://orcid.org/0000-0002-4024-9439"]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiong Wu","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100638184","display_name":"Junming Li","orcid":"https://orcid.org/0009-0001-4940-9793","observed_orcids":["https://orcid.org/0009-0001-4940-9793","https://orcid.org/0000-0003-1409-3634"]},"institutions":[{"id":"https://openalex.org/I3018397418","display_name":"Yuhuangding Hospital","ror":"https://ror.org/05vawe413","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I3018397418"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Junming Li","raw_affiliation_strings":["Department of Clinical Laboratory, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Laboratory, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China","institution_ids":["https://openalex.org/I3018397418"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112913633","display_name":"Yang Zheng","orcid":"https://orcid.org/0000-0001-5680-0814","observed_orcids":["https://orcid.org/0000-0001-5680-0814"]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yang Zheng","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5148472402","display_name":"Xuejun Hu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210092944","display_name":"Dalian University","ror":"https://ror.org/00g2ypp58","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210092944"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xuejun Hu","raw_affiliation_strings":["Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China","institution_ids":["https://openalex.org/I4210092944"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5148472402","https://openalex.org/A5148564734"],"corresponding_institution_ids":["https://openalex.org/I4210092944"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"32","issue":"4","first_page":"1","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.020400000736117363,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nanobodies","display_name":"nanobodies","score":0.9660000205039978},{"id":"https://openalex.org/keywords/c-reactive-protein","display_name":"C-reactive protein","score":0.9089999794960022},{"id":"https://openalex.org/keywords/self-assembling-peptides","display_name":"self-assembling peptides","score":0.8230000138282776},{"id":"https://openalex.org/keywords/c4b-binding-protein","display_name":"C4b-binding protein","score":0.9779999852180481},{"id":"https://openalex.org/keywords/alkaline-phosphatase-fusion","display_name":"alkaline phosphatase fusion","score":0.8190000057220459},{"id":"https://openalex.org/keywords/binding-affinity-enhancement","display_name":"binding affinity enhancement","score":0.7860000133514404},{"id":"https://openalex.org/keywords/competitive-elisa","display_name":"competitive ELISA","score":0.671999990940094},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.8870000243186951},{"id":"https://openalex.org/keywords/protein-thermostability","display_name":"protein thermal stability","score":0.5550000071525574},{"id":"https://openalex.org/keywords/point-of-care-testing","display_name":"point-of-care testing","score":0.5139999985694885},{"id":"https://openalex.org/keywords/staphylothermus-marinus","display_name":"Staphylothermus marinus","score":0.7310000061988831},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7990000247955322}],"concepts":[{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.7415000200271606},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6442999839782715},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5472999811172485},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.5184000134468079},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5090000033378601},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5069000124931335},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5020999908447266},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.48590001463890076},{"id":"https://openalex.org/C160160445","wikidata":"https://www.wikidata.org/wiki/Q24769688","display_name":"Alkaline phosphatase","level":3,"score":0.4616999924182892},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.41850000619888306},{"id":"https://openalex.org/C85528070","wikidata":"https://www.wikidata.org/wiki/Q643602","display_name":"Protein tyrosine phosphatase","level":3,"score":0.41510000824928284},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.3808000087738037},{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.37450000643730164},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.36160001158714294},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.3564000129699707},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34880000352859497},{"id":"https://openalex.org/C2776490512","wikidata":"https://www.wikidata.org/wiki/Q21151790","display_name":"Prostatic acid phosphatase","level":4,"score":0.3303999900817871},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.319599986076355},{"id":"https://openalex.org/C2777366897","wikidata":"https://www.wikidata.org/wiki/Q847482","display_name":"HeLa","level":3,"score":0.31859999895095825},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.3098999857902527},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.30889999866485596},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.29100000858306885},{"id":"https://openalex.org/C55988834","wikidata":"https://www.wikidata.org/wiki/Q903650","display_name":"Coiled coil","level":2,"score":0.29019999504089355},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.28119999170303345},{"id":"https://openalex.org/C2780430158","wikidata":"https://www.wikidata.org/wiki/Q7685902","display_name":"Target protein","level":3,"score":0.28110000491142273},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.2791000008583069},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.26080000400543213},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.2581000030040741},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.25220000743865967},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.25200000405311584},{"id":"https://openalex.org/C59061564","wikidata":"https://www.wikidata.org/wiki/Q7783071","display_name":"Thermal stability","level":2,"score":0.251800000667572},{"id":"https://openalex.org/C2780676925","wikidata":"https://www.wikidata.org/wiki/Q24741276","display_name":"Maltose-binding protein","level":5,"score":0.25099998712539673}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.3892/etm.2026.13279","doi":"https://doi.org/10.3892/etm.2026.13279","is_oa":true,"landing_page_url":"https://doi.org/10.3892/etm.2026.13279","pdf_url":"http://www.spandidos-publications.com/10.3892/etm.2026.13279/download","source":{"id":"https://openalex.org/S151424340","display_name":"Experimental and Therapeutic Medicine","issn_l":"1792-0981","issn":["1792-0981","1792-1015"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","poland-40","vabb-shw"],"host_organization":"https://openalex.org/P4310320412","host_organization_name":"Spandidos Publishing","host_organization_lineage":["https://openalex.org/P4310320412"],"host_organization_lineage_names":["Spandidos Publishing"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental and Therapeutic Medicine","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13551084","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13551084/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13551084/pdf/etm-32-04-13279.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Exp Ther Med","raw_type":"Text"},{"id":"pmid:42713007","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42713007","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental and therapeutic medicine","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3892/etm.2026.13279","doi":"https://doi.org/10.3892/etm.2026.13279","is_oa":true,"landing_page_url":"https://doi.org/10.3892/etm.2026.13279","pdf_url":"http://www.spandidos-publications.com/10.3892/etm.2026.13279/download","source":{"id":"https://openalex.org/S151424340","display_name":"Experimental and Therapeutic Medicine","issn_l":"1792-0981","issn":["1792-0981","1792-1015"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","poland-40","vabb-shw"],"host_organization":"https://openalex.org/P4310320412","host_organization_name":"Spandidos Publishing","host_organization_lineage":["https://openalex.org/P4310320412"],"host_organization_lineage_names":["Spandidos Publishing"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental and Therapeutic Medicine","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.7246000170707703}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8460675958","display_name":null,"funder_award_id":"32270985","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320336584","display_name":"Dalian Science and Technology Innovation Fund","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204498441.pdf","grobid_xml":"https://content.openalex.org/works/W7204498441.grobid-xml"},"referenced_works_count":41,"referenced_works":["https://openalex.org/W1967858111","https://openalex.org/W2007954588","https://openalex.org/W2012009619","https://openalex.org/W2019586872","https://openalex.org/W2042940792","https://openalex.org/W2052524614","https://openalex.org/W2057278101","https://openalex.org/W2088729407","https://openalex.org/W2147636934","https://openalex.org/W2149624141","https://openalex.org/W2336579939","https://openalex.org/W2343861500","https://openalex.org/W2399280670","https://openalex.org/W2770881248","https://openalex.org/W2787437645","https://openalex.org/W2796306345","https://openalex.org/W2885461132","https://openalex.org/W2895070686","https://openalex.org/W2900800154","https://openalex.org/W2909560658","https://openalex.org/W2931491674","https://openalex.org/W2979574851","https://openalex.org/W2983988502","https://openalex.org/W3017389773","https://openalex.org/W3036414432","https://openalex.org/W3036507685","https://openalex.org/W3085261261","https://openalex.org/W3087474741","https://openalex.org/W3128835374","https://openalex.org/W3135831599","https://openalex.org/W3152669539","https://openalex.org/W3173583905","https://openalex.org/W3175192823","https://openalex.org/W3177184182","https://openalex.org/W3192472718","https://openalex.org/W3217630435","https://openalex.org/W4200125817","https://openalex.org/W4205669486","https://openalex.org/W4220686086","https://openalex.org/W4241188448","https://openalex.org/W4317436893"],"related_works":["https://openalex.org/W4407324525","https://openalex.org/W4319442922","https://openalex.org/W4200125817","https://openalex.org/W1542207885","https://openalex.org/W7124715959","https://openalex.org/W4404353989","https://openalex.org/W4285222087","https://openalex.org/W4406887845","https://openalex.org/W2966194992","https://openalex.org/W3003701292"],"abstract_inverted_index":{"Nanobodies":[0],"(Nbs)":[1],"have":[2],"been":[3],"extensively":[4],"utilized":[5],"in":[6,25,49,152,273],"medical":[7],"diagnosis":[8],"and":[9,21,105,130,142,159,194,200,226,231,260,286,293],"therapies":[10],"due":[11],"to":[12,32,34,56,63,74,124,255],"their":[13,29,184],"advantages,":[14],"such":[15,80],"as":[16,81,148,280],"ease":[17],"of":[18,40,61,77,92,101,127,137,173,300],"genetic":[19],"manipulation":[20],"efficient":[22],"soluble":[23,149],"expression":[24,165],"prokaryotic":[26],"systems.However,":[27],"enhancing":[28],"binding":[30,59],"affinity":[31,60,202],"antigens":[33,64,285],"match":[35],"or":[36],"even":[37],"exceed":[38],"that":[39],"polyclonal":[41],"antibodies":[42,62],"for":[43,242,258,283,290],"high-sensitivity":[44],"bioassays":[45],"remains":[46],"a":[47,53,88,180,189,210,220,266,297],"challenge.Therefore,":[48],"the":[50,58,75,99,125,135,153,171,174,205,245,274],"present":[51,275],"study,":[52],"novel":[54,267,298],"strategy":[55],"enhance":[57],"was":[65,121,166,177,186,271],"developed":[66],"by":[67,161,168],"fusing":[68],"an":[69,93],"anti-C-reactive":[70],"protein":[71,107,114,164,269],"Nb":[72],"(CRPNb)":[73],"N-terminus":[76],"self-assembling":[78],"peptides":[79],"right-handed":[82,89],"coiled":[83],"coil":[84],"[RHCC;":[85],"derived":[86,109],"from":[87,110],"coiled-coil":[90],"peptide":[91],"archaebacterium":[94],"(Staphylothermus":[95],"marinus)],":[96],"verotoxin":[97],"(VTB;":[98],"B-subunit":[100],"Escherichia":[102],"coli":[103,155],"verotoxin)":[104],"C4-binding":[106,113],"(C4bp;":[108],"human":[111],"plasma":[112],"\u03b1-chain).For":[115],"functional":[116],"detection,":[117],"alkaline":[118],"phosphatase":[119],"(AP)":[120],"further":[122],"fused":[123],"C-terminus":[126],"RHCC,":[128],"VTB":[129],"C4bp,":[131],"respectively.This":[132],"approach":[133],"enabled":[134],"formation":[136],"CRPNb-RHCC-AP":[138,195,227],"tetramers,":[139],"CRPNb-VTB-AP":[140,192],"pentamers":[141,193],"CRPNb-C4bp-AP":[143],"heptamers.These":[144],"were":[145],"then":[146],"expressed":[147],"cytoplasmic":[150],"proteins":[151,278],"E.":[154],"strain":[156],"BL21":[157],"(DE3)":[158],"purified":[160],"imidazole":[162],"elution.The":[163],"assessed":[167,187],"western":[169],"blotting.Additionally,":[170],"stability":[172,217],"multimeric":[175],"constructs":[176],"evaluated":[178],"using":[179,188],"direct":[181,211,221],"ELISA,":[182],"whilst":[183],"sensitivity":[185],"competitive":[190],"ELISA.The":[191],"tetramers":[196],"demonstrated":[197],"antigen-specific":[198],"recognition":[199],"enhanced":[201],"compared":[203],"with":[204],"CRP-AP":[206],"monomer":[207],"based":[208,218],"on":[209,219],"ELISA.Furthermore,":[212],"they":[213],"exhibited":[214],"good":[215],"thermal":[216],"ELISA.At":[222],"80\u02daC,":[223],"CRPNb-VTB-AP,":[224],"CRPNb-AP":[225],"retained":[228,237],"~85,":[229],"75":[230],"60%":[232],"activity,":[233],"while":[234],"CRPNb-H":[235],"only":[236],"~40%.After":[238],"incubation":[239],"at":[240,262],"80\u02daC":[241],"55":[243],"min,":[244],"two":[246],"multimers":[247],"still":[248],"maintained":[249],"~40%":[250],"activity.The":[251],"results":[252],"suggested":[253],"them":[254],"be":[256],"suitable":[257],"storage":[259],"transportation":[261],"ambient":[263],"temperatures.In":[264],"conclusion,":[265],"nanobody-fusion":[268],"platform":[270],"established":[272],"study.The":[276],"fusion":[277],"functioned":[279],"high-affinity":[281],"binders":[282],"target":[284],"heat-stable":[287],"signal":[288],"tracers":[289],"immunoassay":[291],"detection":[292],"quantitative":[294],"analysis,":[295],"providing":[296],"type":[299],"thermostable":[301],"immunoreagent.":[302]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-08-28T00:00:00"}
