{"id":"https://openalex.org/W7204490128","doi":"https://doi.org/10.64898/2026.08.26.747334","title":"Natural Variation in Maize Shikimate Dehydrogenase Alters Enzyme Activity and Kernel Homoserine Accumulation","display_name":"Natural Variation in Maize Shikimate Dehydrogenase Alters Enzyme Activity and Kernel Homoserine Accumulation","publication_year":2026,"publication_date":"2026-08-27","ids":{"openalex":"https://openalex.org/W7204490128","doi":"https://doi.org/10.64898/2026.08.26.747334"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.26.747334","doi":"https://doi.org/10.64898/2026.08.26.747334","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.26.747334","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/27/2026.08.26.747334.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/27/2026.08.26.747334.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036826984","display_name":"Rajnee Hasan","orcid":"https://orcid.org/0000-0002-9260-050X","observed_orcids":["https://orcid.org/0000-0002-9260-050X"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajnee Hasan","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0002-9260-050X","affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085102532","display_name":"Gen Xu","orcid":"https://orcid.org/0000-0001-5701-4751","observed_orcids":["https://orcid.org/0000-0001-5701-4751"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gen Xu","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0001-5701-4751","affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073115365","display_name":"Taiwo Adeolu Dele-Osibanjo","orcid":"https://orcid.org/0000-0003-2537-6998","observed_orcids":["https://orcid.org/0000-0003-2537-6998"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Taiwo Dele-Osibanjo","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0003-2537-6998","affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087924552","display_name":"Niaz Bahar Chowdhury","orcid":"https://orcid.org/0009-0005-3833-6380","observed_orcids":["https://orcid.org/0009-0005-3833-6380"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Niaz Bahar Chowdhury","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0009-0005-3833-6380","affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006628905","display_name":"Connor Pedersen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Connor Pedersen","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089064325","display_name":"Rajib Saha","orcid":"https://orcid.org/0000-0002-2974-0243","observed_orcids":["https://orcid.org/0000-0002-2974-0243"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rajib Saha","raw_affiliation_strings":["Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0002-2974-0243","affiliations":[{"raw_affiliation_string":"Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006569282","display_name":"Jinliang Yang","orcid":"https://orcid.org/0000-0002-0999-3518","observed_orcids":["https://orcid.org/0000-0002-0999-3518"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jinliang Yang","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0002-0999-3518","affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5080580916","display_name":"Toshihiro Obata","orcid":"https://orcid.org/0000-0001-8931-7722","observed_orcids":["https://orcid.org/0000-0001-8931-7722"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Toshihiro Obata","raw_affiliation_strings":["Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"],"raw_orcid":"https://orcid.org/0000-0001-8931-7722","affiliations":[{"raw_affiliation_string":"Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]},{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA","institution_ids":["https://openalex.org/I114395901"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5080580916"],"corresponding_institution_ids":["https://openalex.org/I114395901"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.8628000020980835,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.8628000020980835,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.07819999754428864,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11237","display_name":"Plant Gene Expression Analysis","score":0.010300000198185444,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/grain-corn","display_name":"maize grain","score":0.8119999766349792},{"id":"https://openalex.org/keywords/shikimate-dehydrogenase","display_name":"shikimate dehydrogenase","score":0.9819999933242798},{"id":"https://openalex.org/keywords/sad1","display_name":"SAD1","score":0.8569999933242798},{"id":"https://openalex.org/keywords/natural-genetic-variation","display_name":"natural genetic variation","score":0.6790000200271606},{"id":"https://openalex.org/keywords/amino-acid-biosynthesis","display_name":"amino acid biosynthesis","score":0.7080000042915344},{"id":"https://openalex.org/keywords/shikimate-pathway","display_name":"shikimate pathway","score":0.8679999709129333},{"id":"https://openalex.org/keywords/redox-metabolism","display_name":"redox metabolism","score":0.9380000233650208},{"id":"https://openalex.org/keywords/oxaloacetate","display_name":"oxaloacetate","score":0.7170000076293945},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5389999747276306},{"id":"https://openalex.org/keywords/haplotypes","display_name":"haplotypes","score":0.7749999761581421},{"id":"https://openalex.org/keywords/non-synonymous-variants","display_name":"non-synonymous variants","score":0.6919999718666077},{"id":"https://openalex.org/keywords/maize-inbred-lines","display_name":"maize inbred lines","score":0.6610000133514404}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7279000282287598},{"id":"https://openalex.org/C2778255928","wikidata":"https://www.wikidata.org/wiki/Q858525","display_name":"Shikimate pathway","level":4,"score":0.7225000262260437},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5898000001907349},{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.5620999932289124},{"id":"https://openalex.org/C2779840497","wikidata":"https://www.wikidata.org/wiki/Q418214","display_name":"Homoserine","level":5,"score":0.544700026512146},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.5116999745368958},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.4876999855041504},{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.477400004863739},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.460099995136261},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.451200008392334},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.4447999894618988},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4341999888420105},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.38449999690055847},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.36800000071525574},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.35269999504089355},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.3402000069618225},{"id":"https://openalex.org/C188142797","wikidata":"https://www.wikidata.org/wiki/Q3231893","display_name":"Amino acid synthesis","level":4,"score":0.32670000195503235},{"id":"https://openalex.org/C2777992278","wikidata":"https://www.wikidata.org/wiki/Q705605","display_name":"Aromatic amino acids","level":3,"score":0.31360000371932983},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.3084000051021576},{"id":"https://openalex.org/C68873052","wikidata":"https://www.wikidata.org/wiki/Q349856","display_name":"Genetic variation","level":3,"score":0.29789999127388},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.26989999413490295},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.26910001039505005},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.26179999113082886},{"id":"https://openalex.org/C2781374117","wikidata":"https://www.wikidata.org/wiki/Q5252382","display_name":"Dehydratase","level":3,"score":0.25380000472068787}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.26.747334","doi":"https://doi.org/10.64898/2026.08.26.747334","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.26.747334","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/27/2026.08.26.747334.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.26.747334","doi":"https://doi.org/10.64898/2026.08.26.747334","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.26.747334","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/27/2026.08.26.747334.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320332299","display_name":"National Institute of Food and Agriculture","ror":"https://ror.org/05qx3fv49"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204490128.pdf","grobid_xml":"https://content.openalex.org/works/W7204490128.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W1980168725","https://openalex.org/W1999083718","https://openalex.org/W2008781848","https://openalex.org/W2015600749","https://openalex.org/W2019402080","https://openalex.org/W2032700288","https://openalex.org/W2044243244","https://openalex.org/W2052874938","https://openalex.org/W2063762505","https://openalex.org/W2068406373","https://openalex.org/W2070853125","https://openalex.org/W2072171662","https://openalex.org/W2074783354","https://openalex.org/W2075996985","https://openalex.org/W2078355959","https://openalex.org/W2078870977","https://openalex.org/W2099085143","https://openalex.org/W2101284429","https://openalex.org/W2110035718","https://openalex.org/W2116882280","https://openalex.org/W2121769890","https://openalex.org/W2124019798","https://openalex.org/W2127543426","https://openalex.org/W2134036574","https://openalex.org/W2142486812","https://openalex.org/W2159295476","https://openalex.org/W2344648730","https://openalex.org/W2749410844","https://openalex.org/W2792771548","https://openalex.org/W2792920971","https://openalex.org/W2796142352","https://openalex.org/W2800434159","https://openalex.org/W2800524954","https://openalex.org/W2809393279","https://openalex.org/W2896828227","https://openalex.org/W2953883853","https://openalex.org/W2999590229","https://openalex.org/W3004925687","https://openalex.org/W3118580617","https://openalex.org/W3201597199","https://openalex.org/W4231096068","https://openalex.org/W4288214500","https://openalex.org/W4292702117","https://openalex.org/W4318754982","https://openalex.org/W4318824132","https://openalex.org/W4387114399","https://openalex.org/W4388461873","https://openalex.org/W4390561755","https://openalex.org/W4396576864","https://openalex.org/W4415522568"],"related_works":["https://openalex.org/W2103476517","https://openalex.org/W4409670987","https://openalex.org/W2121769890","https://openalex.org/W2048508052","https://openalex.org/W2790877627","https://openalex.org/W2094616529","https://openalex.org/W4416979190","https://openalex.org/W1979541972","https://openalex.org/W2460454277","https://openalex.org/W4322770963"],"abstract_inverted_index":{"Abstract":[0],"Metabolic":[1],"diversity":[2],"in":[3,54,151,195],"maize":[4,56,196],"kernels":[5],"determines":[6],"nutritional":[7],"quality":[8],"and":[9,22,42,127,138,141,160,181,191],"end-use":[10],"value.":[11],"Therefore,":[12],"understanding":[13],"its":[14],"genetic":[15,48],"basis":[16],"is":[17],"essential":[18],"for":[19,90,158],"crop":[20],"improvement":[21],"elucidating":[23],"plant":[24],"metabolic":[25,44,85,146],"regulation.":[26],"Here,":[27],"we":[28],"integrated":[29],"metabolite":[30,52],"profiling":[31],"with":[32,66,116],"metabolite-based":[33],"genome-wide":[34],"association":[35,89],"studies":[36],"(mGWAS),":[37],"structural":[38],"modeling,":[39],"enzyme":[40,129,179],"kinetics,":[41],"genome-scale":[43],"simulations":[45],"to":[46,93],"identify":[47],"determinants":[49],"of":[50,60,121],"kernel":[51],"variation":[53,150,177],"265":[55],"inbred":[57],"lines.":[58],"Profiling":[59],"57":[61],"metabolites":[62],"revealed":[63],"inter-genotypic":[64],"variation,":[65],"homoserine":[67,91,118,161],"among":[68],"the":[69,94,167,186],"most":[70],"variable":[71],"metabolites.":[72],"mGWAS":[73],"identified":[74],"62":[75],"locus\u2013trait":[76],"associations":[77],"implicating":[78],"788":[79],"candidate":[80],"genes,":[81],"including":[82,107],"154":[83],"encoding":[84],"enzymes.":[86],"A":[87],"major":[88],"mapped":[92],"shikimate":[95,187],"dehydrogenase":[96],"gene":[97,122],"Sad1":[98,113,175],"on":[99],"chromosome":[100],"10.":[101],"Four":[102],"tightly":[103],"linked":[104],"coding-region":[105],"SNPs,":[106],"three":[108],"non-synonymous":[109],"variants,":[110],"defined":[111],"two":[112],"haplotypes":[114],"associated":[115],"differential":[117],"accumulation,":[119,184],"independent":[120],"expression":[123],"variation.":[124],"Structural":[125],"analysis":[126],"recombinant":[128],"assays":[130],"showed":[131],"that":[132,149,174],"these":[133],"substitutions":[134],"occur":[135],"within":[136],"catalytic":[137,143],"cofactor-binding":[139],"domains":[140],"alter":[142],"efficiency.":[144],"Genome-scale":[145],"modeling":[147],"indicated":[148],"SAD1":[152],"activity":[153],"influences":[154],"plastidial":[155],"oxaloacetate":[156],"availability":[157],"aspartate":[159],"biosynthesis":[162,194],"through":[163],"redox-coupled":[164],"flux":[165],"via":[166],"malate-oxaloacetate":[168],"shuttle.":[169],"Together,":[170],"our":[171],"results":[172],"indicate":[173],"allelic":[176],"alters":[178],"function":[180],"amino":[182,192],"acid":[183,193],"linking":[185],"pathway,":[188],"redox":[189],"metabolism,":[190],"kernels.":[197]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-08-28T00:00:00"}