{"id":"https://openalex.org/W7204264526","doi":"https://doi.org/10.5281/zenodo.22107279","title":"Exploring the Fundamentals of Molecular Biology: From DNA Extraction to Gene Cloning and Biochemical Analysis","display_name":"Exploring the Fundamentals of Molecular Biology: From DNA Extraction to Gene Cloning and Biochemical Analysis","publication_year":2025,"publication_date":"2025-02-01","ids":{"openalex":"https://openalex.org/W7204264526","doi":"https://doi.org/10.5281/zenodo.22107279"},"language":"en","primary_location":{"id":"doi:10.5281/zenodo.22107279","doi":"https://doi.org/10.5281/zenodo.22107279","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.22107279","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.5281/zenodo.22107279","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5107405171","display_name":"Aman Tiple","orcid":"https://orcid.org/0009-0008-6747-2244","observed_orcids":["https://orcid.org/0009-0008-6747-2244"]},"institutions":[{"id":"https://openalex.org/I4405267752","display_name":"The Leprosy Mission Trust India","ror":"https://ror.org/046a6pa59","country_code":"IN","type":"nonprofit","lineage":["https://openalex.org/I4405267752"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Tiple, Aman","raw_affiliation_strings":["The Leprosy Mission Trust India"],"raw_orcid":"https://orcid.org/0009-0008-6747-2244","affiliations":[{"raw_affiliation_string":"The Leprosy Mission Trust India","institution_ids":["https://openalex.org/I4405267752"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5107405171"],"corresponding_institution_ids":["https://openalex.org/I4405267752"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.9869999885559082},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.7300000190734863},{"id":"https://openalex.org/keywords/pcr","display_name":"PCR","score":0.7149999737739563},{"id":"https://openalex.org/keywords/gel-electrophoresis","display_name":"gel electrophoresis","score":0.9909999966621399},{"id":"https://openalex.org/keywords/gram-stain","display_name":"Gram staining","score":0.9190000295639038},{"id":"https://openalex.org/keywords/restriction-enzyme-digestion","display_name":"restriction enzyme digestion","score":0.722000002861023},{"id":"https://openalex.org/keywords/pcr-cloning","display_name":"PCR cloning","score":0.8330000042915344},{"id":"https://openalex.org/keywords/sugarcane","display_name":"sugarcane","score":0.6700000166893005},{"id":"https://openalex.org/keywords/fish-gut-microbiota","display_name":"fish gut microbiota","score":0.996999979019165},{"id":"https://openalex.org/keywords/bacterial-identification","display_name":"bacterial identification","score":0.7039999961853027},{"id":"https://openalex.org/keywords/primer-specificity","display_name":"primer specificity","score":0.6970000267028809},{"id":"https://openalex.org/keywords/real-time-pcr","display_name":"real-time PCR","score":0.859000027179718},{"id":"https://openalex.org/keywords/nucleic-acid-purification","display_name":"nucleic acid purification","score":0.9380000233650208}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.703000009059906},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5989000201225281},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.5942000150680542},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.5246000289916992},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.5217999815940857},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5069000124931335},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4731000065803528},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4562999904155731},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4487999975681305},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.44519999623298645},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4180999994277954},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3822000026702881},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.3792000114917755},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.36390000581741333},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.35589998960494995},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.349700003862381},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.34470000863075256},{"id":"https://openalex.org/C28134486","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Applications of PCR","level":5,"score":0.33649998903274536},{"id":"https://openalex.org/C126307556","wikidata":"https://www.wikidata.org/wiki/Q4227909","display_name":"Nucleic acid quantitation","level":3,"score":0.3188999891281128},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3100000023841858},{"id":"https://openalex.org/C156385826","wikidata":"https://www.wikidata.org/wiki/Q1341059","display_name":"Multiple displacement amplification","level":5,"score":0.2924000024795532},{"id":"https://openalex.org/C153289436","wikidata":"https://www.wikidata.org/wiki/Q3826844","display_name":"Nucleic acid methods","level":3,"score":0.27649998664855957},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.26919999718666077},{"id":"https://openalex.org/C204315192","wikidata":"https://www.wikidata.org/wiki/Q210506","display_name":"Molecular genetics","level":3,"score":0.25589999556541443},{"id":"https://openalex.org/C145169222","wikidata":"https://www.wikidata.org/wiki/Q9339485","display_name":"Molecular probe","level":3,"score":0.25270000100135803},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.25049999356269836}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.5281/zenodo.22107279","doi":"https://doi.org/10.5281/zenodo.22107279","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.22107279","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.5281/zenodo.22107280","doi":"https://doi.org/10.5281/zenodo.22107280","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.22107280","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.5281/zenodo.22107279","doi":"https://doi.org/10.5281/zenodo.22107279","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.22107279","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7201841716","https://openalex.org/W7208836438","https://openalex.org/W7117676120","https://openalex.org/W4417067674","https://openalex.org/W6930514630","https://openalex.org/W4245970768","https://openalex.org/W2390278618","https://openalex.org/W7038734223","https://openalex.org/W2349552057","https://openalex.org/W320993225"],"abstract_inverted_index":{"Molecular":[0],"biology":[1,146],"is":[2,51,70],"essential":[3],"for":[4,44,76,122],"understanding":[5],"biological":[6],"systems,":[7],"with":[8],"DNA":[9,32],"extraction,":[10],"polymerase":[11],"chain":[12],"reaction":[13],"(PCR)":[14],"analysis,":[15,55],"gel":[16],"electrophoresis,":[17],"and":[18,36,41,58,64,80,89,100,117,127,138,150],"microbial":[19,107],"biochemical":[20,90],"testing":[21],"serving":[22],"as":[23,72,86],"key":[24],"methodologies.":[25],"This":[26,141],"study":[27,142],"explores":[28],"the":[29],"extraction":[30],"of":[31],"from":[33],"bacterial,":[34],"fungal,":[35],"plant":[37],"samples,":[38],"highlighting":[39],"challenges":[40,132],"optimized":[42],"protocols":[43],"high-quality":[45],"nucleic":[46,135],"acid":[47,136],"retrieval.":[48],"PCR":[49,66,78],"analysis":[50],"examined":[52],"in":[53,93,97,125,134],"genetic":[54,81],"disease":[56],"detection,":[57],"molecular":[59,145],"cloning,":[60,119],"emphasizing":[61],"primer":[62],"specificity":[63],"real-time":[65],"advancements.":[67],"Gel":[68],"electrophoresis":[69],"discussed":[71],"a":[73],"crucial":[74],"tool":[75],"verifying":[77],"products":[79],"modifications.":[82],"Microbiological":[83],"techniques":[84],"such":[85],"Gram":[87],"staining":[88],"assays":[91],"aid":[92],"bacterial":[94],"identification,":[95],"particularly":[96],"sugarcane":[98],"tissues":[99],"fish":[101],"gut":[102],"microbiota,":[103],"providing":[104],"insights":[105,154],"into":[106,155],"interactions.":[108],"Gene":[109],"cloning":[110],"methodologies,":[111],"including":[112],"restriction":[113],"enzyme":[114],"digestion,":[115],"ligation,":[116],"PCR-based":[118],"are":[120],"explored":[121],"their":[123,148],"applications":[124],"biotechnology":[126],"medical":[128],"research.":[129],"Despite":[130],"advancements,":[131],"persist":[133],"purification":[137],"amplification":[139],"efficiency.":[140],"summarizes":[143],"fundamental":[144],"techniques,":[147],"applications,":[149],"ongoing":[151],"improvements,":[152],"offering":[153],"future":[156],"research":[157],"directions.":[158]},"counts_by_year":[],"updated_date":"2026-10-07T09:51:55.459714","created_date":"2026-08-27T00:00:00"}
