{"id":"https://openalex.org/W7204254151","doi":"https://doi.org/10.1007/s00253-026-13997-6","title":"Efficient biotechnological production of N-(Hydroxycinnamoyl)tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation","display_name":"Efficient biotechnological production of N-(Hydroxycinnamoyl)tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation","publication_year":2026,"publication_date":"2026-08-26","ids":{"openalex":"https://openalex.org/W7204254151","doi":"https://doi.org/10.1007/s00253-026-13997-6","pmid":"https://pubmed.ncbi.nlm.nih.gov/42645554"},"language":"en","primary_location":{"id":"doi:10.1007/s00253-026-13997-6","doi":"https://doi.org/10.1007/s00253-026-13997-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-026-13997-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-026-13997-6.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","erih-plus","jufo-2","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://link.springer.com/content/pdf/10.1007/s00253-026-13997-6.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5148384820","display_name":"Israa Abuobida Mohamed","orcid":"https://orcid.org/0009-0009-1559-3252","observed_orcids":["https://orcid.org/0009-0009-1559-3252"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Israa Abuobida Mohamed","raw_affiliation_strings":["Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany"],"raw_orcid":"https://orcid.org/0009-0009-1559-3252","affiliations":[{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148357206","display_name":"Qais Al-Khawlani","orcid":"https://orcid.org/0009-0005-1452-7203","observed_orcids":["https://orcid.org/0009-0005-1452-7203"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Qais Al-Khawlani","raw_affiliation_strings":["Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany"],"raw_orcid":"https://orcid.org/0009-0005-1452-7203","affiliations":[{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079934444","display_name":"Raika Milde","orcid":"https://orcid.org/0000-0003-0630-8105","observed_orcids":["https://orcid.org/0000-0003-0630-8105"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Raika Milde","raw_affiliation_strings":["Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany"],"raw_orcid":"https://orcid.org/0000-0003-0630-8105","affiliations":[{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028145690","display_name":"Niels Valentin Heise","orcid":"https://orcid.org/0000-0002-6540-6357","observed_orcids":["https://orcid.org/0000-0002-6540-6357"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Niels Valentin Heise","raw_affiliation_strings":["Institute of Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Stra\u00dfe 2, 06120, Halle, Germany"],"raw_orcid":"https://orcid.org/0000-0002-6540-6357","affiliations":[{"raw_affiliation_string":"Institute of Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Stra\u00dfe 2, 06120, Halle, Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017208101","display_name":"Martin Dippe","orcid":"https://orcid.org/0000-0001-7123-7812","observed_orcids":["https://orcid.org/0000-0001-7123-7812"]},"institutions":[{"id":"https://openalex.org/I4210110463","display_name":"Leibniz Institute of Plant Biochemistry","ror":"https://ror.org/01mzk5576","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210110463"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Martin Dippe","raw_affiliation_strings":["Leibniz-Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, 06120, Halle, Germany"],"raw_orcid":"https://orcid.org/0000-0001-7123-7812","affiliations":[{"raw_affiliation_string":"Leibniz-Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, 06120, Halle, Germany","institution_ids":["https://openalex.org/I4210110463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068588457","display_name":"Mohamed Nagia","orcid":"https://orcid.org/0000-0002-7280-5153","observed_orcids":["https://orcid.org/0000-0002-7280-5153"]},"institutions":[{"id":"https://openalex.org/I4210131959","display_name":"National Research Centre","ror":"https://ror.org/02n85j827","country_code":"EG","type":"facility","lineage":["https://openalex.org/I4210131959"]},{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE","EG"],"is_corresponding":false,"raw_author_name":"Mohamed Nagia","raw_affiliation_strings":["Department of Chemistry of Natural Compounds, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St. 33, 12622, Cairo, Egypt","Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany"],"raw_orcid":"https://orcid.org/0000-0002-7280-5153","affiliations":[{"raw_affiliation_string":"Department of Chemistry of Natural Compounds, Pharmaceutical and Drug Industries Research Institute, National Research Center, El Buhouth St. 33, 12622, Cairo, Egypt","institution_ids":["https://openalex.org/I4210131959"]},{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049586619","display_name":"Ludger A. Wessjohann","orcid":"https://orcid.org/0000-0003-2060-8235","observed_orcids":["https://orcid.org/0000-0003-2060-8235"]},"institutions":[{"id":"https://openalex.org/I4210110463","display_name":"Leibniz Institute of Plant Biochemistry","ror":"https://ror.org/01mzk5576","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210110463"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ludger A. Wessjohann","raw_affiliation_strings":["Leibniz-Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, 06120, Halle, Germany"],"raw_orcid":"https://orcid.org/0000-0003-2060-8235","affiliations":[{"raw_affiliation_string":"Leibniz-Institute of Plant Biochemistry, Department of Bioorganic Chemistry, Weinberg 3, 06120, Halle, Germany","institution_ids":["https://openalex.org/I4210110463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072147297","display_name":"Markus Pietzsch","orcid":"https://orcid.org/0000-0001-6266-9621","observed_orcids":["https://orcid.org/0000-0001-6266-9621"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Markus Pietzsch","raw_affiliation_strings":["Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany"],"raw_orcid":"https://orcid.org/0000-0001-6266-9621","affiliations":[{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021848130","display_name":"Bodo Moritz","orcid":"https://orcid.org/0009-0005-9339-3409","observed_orcids":["https://orcid.org/0009-0005-9339-3409"]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Bodo Moritz","raw_affiliation_strings":["Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany. bodo.moritz@pharmazie.uni-halle.de"],"raw_orcid":"https://orcid.org/0009-0005-9339-3409","affiliations":[{"raw_affiliation_string":"Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120, Halle (Saale), Germany. bodo.moritz@pharmazie.uni-halle.de","institution_ids":["https://openalex.org/I68956291"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5021848130"],"corresponding_institution_ids":["https://openalex.org/I68956291"],"apc_list":{"value":4190,"currency":"USD","value_usd":4190},"apc_paid":{"value":4190,"currency":"USD","value_usd":4190},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.64079966,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"110","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.012400000356137753,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.005100000184029341,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/feruloyltyramine","display_name":"feruloyltyramine","score":0.968999981880188},{"id":"https://openalex.org/keywords/4-coumarate-coa-ligase","display_name":"4-coumarate:CoA ligase","score":0.9160000085830688},{"id":"https://openalex.org/keywords/plant-enzymes","display_name":"plant enzymes","score":0.6480000019073486},{"id":"https://openalex.org/keywords/recombinant-escherichia-coli","display_name":"recombinant Escherichia coli","score":0.8629999756813049},{"id":"https://openalex.org/keywords/filter-cake","display_name":"filter cake","score":0.44200000166893005},{"id":"https://openalex.org/keywords/bioprocess-optimization","display_name":"bioprocess optimization","score":0.5590000152587891},{"id":"https://openalex.org/keywords/space-time-yield","display_name":"space-time yield","score":0.5410000085830688}],"concepts":[{"id":"https://openalex.org/C4916135","wikidata":"https://www.wikidata.org/wiki/Q4119055","display_name":"Bioprocess","level":2,"score":0.760200023651123},{"id":"https://openalex.org/C168170006","wikidata":"https://www.wikidata.org/wiki/Q864699","display_name":"Bioreactor","level":2,"score":0.7534999847412109},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5895000100135803},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.4851999878883362},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.44510000944137573},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.39899998903274536},{"id":"https://openalex.org/C73447357","wikidata":"https://www.wikidata.org/wiki/Q425079","display_name":"Polyhydroxyalkanoates","level":3,"score":0.34049999713897705},{"id":"https://openalex.org/C67568554","wikidata":"https://www.wikidata.org/wiki/Q385442","display_name":"Downstream processing","level":2,"score":0.33629998564720154},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.3292999863624573},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.32670000195503235},{"id":"https://openalex.org/C2780434155","wikidata":"https://www.wikidata.org/wiki/Q7247767","display_name":"Product inhibition","level":4,"score":0.3264000117778778},{"id":"https://openalex.org/C70899900","wikidata":"https://www.wikidata.org/wiki/Q290439","display_name":"Polyphenol","level":3,"score":0.3188999891281128},{"id":"https://openalex.org/C48314217","wikidata":"https://www.wikidata.org/wiki/Q1753040","display_name":"Microfiltration","level":3,"score":0.31610000133514404},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.31610000133514404},{"id":"https://openalex.org/C175605896","wikidata":"https://www.wikidata.org/wiki/Q39833","display_name":"Microorganism","level":3,"score":0.31540000438690186},{"id":"https://openalex.org/C2780956279","wikidata":"https://www.wikidata.org/wiki/Q577117","display_name":"Pilot plant","level":2,"score":0.3151000142097473},{"id":"https://openalex.org/C2777908453","wikidata":"https://www.wikidata.org/wiki/Q1128255","display_name":"By-product","level":2,"score":0.3041999936103821},{"id":"https://openalex.org/C157670687","wikidata":"https://www.wikidata.org/wiki/Q426933","display_name":"Postharvest","level":2,"score":0.29919999837875366},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.29030001163482666},{"id":"https://openalex.org/C106131492","wikidata":"https://www.wikidata.org/wiki/Q3072260","display_name":"Filter (signal processing)","level":2,"score":0.2766000032424927},{"id":"https://openalex.org/C206139338","wikidata":"https://www.wikidata.org/wiki/Q192355","display_name":"Raw material","level":2,"score":0.26109999418258667},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25290000438690186}],"mesh":[{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003066","descriptor_name":"Coenzyme A Ligases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003373","descriptor_name":"Coumaric Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003373","descriptor_name":"Coumaric Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003373","descriptor_name":"Coumaric Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003373","descriptor_name":"Coumaric Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014439","descriptor_name":"Tyramine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1007/s00253-026-13997-6","doi":"https://doi.org/10.1007/s00253-026-13997-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-026-13997-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-026-13997-6.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","erih-plus","jufo-2","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},{"id":"pmid:42645554","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42645554","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied microbiology and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:aced03a4b52f4979bb07aa1603718911","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/aced03a4b52f4979bb07aa1603718911","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied Microbiology and Biotechnology, Vol 110, Iss 1 (2026)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13518753","doi":null,"is_oa":true,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42645554/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Appl Microbiol Biotechnol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1007/s00253-026-13997-6","doi":"https://doi.org/10.1007/s00253-026-13997-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-026-13997-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s00253-026-13997-6.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","erih-plus","jufo-2","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/12","display_name":"Responsible consumption and production","score":0.807699978351593},{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.5666999816894531}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204254151.pdf","grobid_xml":"https://content.openalex.org/works/W7204254151.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"The":[1,126,173],"efficient":[2],"recovery":[3],"of":[4,12,25,56,99,118,129,163,188,223],"plant":[5,20],"polyphenolic":[6],"bioactives":[7],"is":[8,177,211],"facing":[9],"technological":[10],"challenges":[11],"low":[13,201],"concentrations":[14],"and":[15,61,77,107,180,225],"complex":[16],"mixtures":[17],"starting":[18],"from":[19,148,214],"tissue.":[21],"In":[22,207],"the":[23,130,138,183],"search":[24],"alternatives,":[26],"we":[27,93],"developed":[28,175],"a":[29,74,82,114,149],"high-yield":[30],"biotechnological":[31],"production":[32,151],"process":[33,140,176,227],"for":[34,185],"hydroxycinnamic":[35,191],"acid\u2013tyramine":[36],"conjugates":[37],"in":[38],"Escherichia":[39],"coli":[40],"by":[41,54,72,169],"converting":[42],"soluble":[43],"substrates":[44],"into":[45],"insoluble":[46],"products.":[47],"A":[48],"recombinant":[49],"plant-enzyme":[50],"cascade":[51],"was":[52,132,167],"established":[53],"co-expression":[55],"Arabidopsis":[57],"thaliana":[58],"4-coumarate:CoA":[59],"ligase":[60],"Solanum":[62],"tuberosum":[63],"hydroxycinnamoyl-CoA:tyramine":[64],"N":[65],"-(hydroxycinnamoyl)transferase.":[66],"We":[67],"overcame":[68],"initial":[69],"enzymatic":[70],"limitations":[71],"developing":[73],"temperature":[75,202],"adapted":[76],"sequentially":[78],"phased":[79],"bioprocess":[80],"on":[81],"1":[83],"L":[84],"reactor":[85],"scale.":[86],"With":[87],"5":[88],"mM/h":[89],"substrate":[90],"supply":[91],"rate":[92,220],"achieved":[94],"31.9":[95],"g/L":[96,103,109],"(102":[97],"mM)":[98,105,111],"feruloyltyramine":[100],"(FerT),":[101],"14.4":[102],"(50.7":[104],"coumaroyltyramine,":[106],"14.6":[108],"(46.5":[110],"isoferuloyltyramine":[112],"with":[113],"space":[115],"time":[116],"yield":[117],"1.75":[119],"g":[120],"*L":[121],"\u22121":[122,124],"*h":[123],"FerT.":[125,164],"vast":[127],"majority":[128],"product":[131,210],"obtained":[133],"as":[134],"filterable":[135],"precipitate,":[136],"simplifying":[137],"downstream":[139],"compared":[141],"to":[142],"classical":[143],"procedures.":[144],"Washed":[145],"filter":[146],"cake":[147],"FerT":[150],"after":[152,171],"filtration":[153],"via":[154],"16\u201340":[155],"\u00b5m":[156],"glass":[157],"filter,":[158],"showed":[159],"99%":[160],"mass":[161],"content":[162],"Product":[165],"identity":[166],"confirmed":[168],"NMR":[170],"recrystallization.":[172],"newly":[174],"robust,":[178],"cost-effective":[179],"scalable,":[181],"paving":[182],"way":[184],"commercial":[186],"applications":[187],"biotechnologically":[189],"produced":[190],"acid\u2013tyramines.":[192],"Key":[193],"points":[194],"\u2022":[195,206,217],"Plant":[196],"derived":[197],"enzymes":[198],"expressed":[199],"at":[200],"enable":[203],"high":[204],"productivity":[205],"situ":[208],"precipitated":[209],"readily":[212],"filtered":[213],"fermentation":[215],"broth":[216],"Carbon":[218],"transfer":[219],"mirrors":[221],"toxicity":[222],"phenolics":[224],"guide":[226],"optimization":[228]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2026-08-27T00:00:00"}