{"id":"https://openalex.org/W7204242826","doi":"https://doi.org/10.1016/j.pep.2026.106996","title":"An expression framework for production of recombinant methionine aminopeptidase in Escherichia coli: a case study of upstream process","display_name":"An expression framework for production of recombinant methionine aminopeptidase in Escherichia coli: a case study of upstream process","publication_year":2026,"publication_date":"2026-08-26","ids":{"openalex":"https://openalex.org/W7204242826","doi":"https://doi.org/10.1016/j.pep.2026.106996","pmid":"https://pubmed.ncbi.nlm.nih.gov/42648655"},"language":"en","primary_location":{"id":"doi:10.1016/j.pep.2026.106996","doi":"https://doi.org/10.1016/j.pep.2026.106996","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2026.106996","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085262260","display_name":"George Kuznetsov","orcid":"https://orcid.org/0000-0002-2599-2689","observed_orcids":["https://orcid.org/0000-0002-2599-2689"]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"George Kuznetsov","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia. Electronic address: Georgii.Kuznetsov@geropharm.com"],"raw_orcid":"https://orcid.org/0000-0002-2599-2689","affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia. Electronic address: Georgii.Kuznetsov@geropharm.com","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148383855","display_name":"Marina Yarovikova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Marina Yarovikova","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148205023","display_name":"Evgenia Buslaeva","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Evgenia Buslaeva","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5123182463","display_name":"Maria Bochkareva","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Maria Bochkareva","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148332884","display_name":"Dilara Salimova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Dilara Salimova","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148292152","display_name":"Ekaterina Tilga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Ekaterina Tilga","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003269586","display_name":"\u0417. \u0420. \u0425\u0430\u0441\u0430\u043d\u0448\u0438\u043d\u0430","orcid":"https://orcid.org/0000-0002-0065-1853","observed_orcids":["https://orcid.org/0000-0002-0065-1853"]},"institutions":[{"id":"https://openalex.org/I173089394","display_name":"ITMO University","ror":"https://ror.org/04txgxn49","country_code":"RU","type":"education","lineage":["https://openalex.org/I173089394"]},{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Zukhra Khasanshina","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia; ITMO University, Russia. Electronic address: Hasanshinazuhrar@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia; ITMO University, Russia. Electronic address: Hasanshinazuhrar@gmail.com","institution_ids":["https://openalex.org/I173089394","https://openalex.org/I4405267459"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022584888","display_name":"\u0414\u0440\u0430\u0439 \u0420\u043e\u043c\u0430\u043d \u0412\u0430\u0441\u0438\u043b\u044c\u0435\u0432\u0438\u0447","orcid":"https://orcid.org/0000-0003-4594-6097","observed_orcids":["https://orcid.org/0000-0003-4594-6097"]},"institutions":[{"id":"https://openalex.org/I4405267459","display_name":"GEROPHARM (Russia)","ror":"https://ror.org/02vkqd817","country_code":"RU","type":"company","lineage":["https://openalex.org/I4405267459"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Roman Dray","raw_affiliation_strings":["Pharm-Holding (RnD Geropharm), Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharm-Holding (RnD Geropharm), Russia","institution_ids":["https://openalex.org/I4405267459"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5003269586","https://openalex.org/A5085262260"],"corresponding_institution_ids":["https://openalex.org/I173089394","https://openalex.org/I4405267459"],"apc_list":{"value":3680,"currency":"USD","value_usd":3680},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"243","issue":null,"first_page":"106996","last_page":"106996"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8711000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8711000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.03790000081062317,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.02850000001490116,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/methionine-aminopeptidase","display_name":"methionine aminopeptidase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.890999972820282},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8029999732971191},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.847000002861023},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.8100000023841858},{"id":"https://openalex.org/keywords/escherichia-coli-bl21-de3","display_name":"Escherichia coli BL21(DE3)","score":0.8489999771118164},{"id":"https://openalex.org/keywords/upstream-process","display_name":"upstream process","score":0.44699999690055847},{"id":"https://openalex.org/keywords/acetate-metabolism","display_name":"acetate metabolism","score":0.6190000176429749}],"concepts":[{"id":"https://openalex.org/C2780912031","wikidata":"https://www.wikidata.org/wiki/Q180341","display_name":"Methionine","level":3,"score":0.7562999725341797},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7559000253677368},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7084000110626221},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6478000283241272},{"id":"https://openalex.org/C2780985610","wikidata":"https://www.wikidata.org/wiki/Q419527","display_name":"Aminopeptidase","level":4,"score":0.5737000107765198},{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.5709999799728394},{"id":"https://openalex.org/C2909420578","wikidata":"https://www.wikidata.org/wiki/Q6823653","display_name":"Methionine Adenosyltransferase","level":4,"score":0.5540000200271606},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5073000192642212},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4848000109195709},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.36419999599456787},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.34450000524520874},{"id":"https://openalex.org/C54374919","wikidata":"https://www.wikidata.org/wiki/Q421015","display_name":"Exopeptidase","level":3,"score":0.33320000767707825},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.32499998807907104},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32030001282691956},{"id":"https://openalex.org/C90061646","wikidata":"https://www.wikidata.org/wiki/Q426173","display_name":"Proinsulin","level":3,"score":0.2987000048160553},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.26739999651908875},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.260699987411499},{"id":"https://openalex.org/C2780868878","wikidata":"https://www.wikidata.org/wiki/Q1660197","display_name":"Immunogenicity","level":3,"score":0.25119999051094055}],"mesh":[{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D000626","descriptor_name":"Aminopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002479","descriptor_name":"Inclusion Bodies","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008666","descriptor_name":"Metalloendopeptidases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008666","descriptor_name":"Metalloendopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D063208","descriptor_name":"Methionyl Aminopeptidases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D063208","descriptor_name":"Methionyl Aminopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.pep.2026.106996","doi":"https://doi.org/10.1016/j.pep.2026.106996","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2026.106996","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:42648655","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42648655","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W1604409283","https://openalex.org/W1915519529","https://openalex.org/W1970756887","https://openalex.org/W1980740090","https://openalex.org/W2007807541","https://openalex.org/W2026093126","https://openalex.org/W2030680112","https://openalex.org/W2033134248","https://openalex.org/W2039260851","https://openalex.org/W2075785824","https://openalex.org/W2077139238","https://openalex.org/W2078472670","https://openalex.org/W2086652584","https://openalex.org/W2091053076","https://openalex.org/W2097893893","https://openalex.org/W2102267945","https://openalex.org/W2106246537","https://openalex.org/W2130091680","https://openalex.org/W2138820253","https://openalex.org/W2147533449","https://openalex.org/W2157815481","https://openalex.org/W2159827108","https://openalex.org/W2161273461","https://openalex.org/W2166744717","https://openalex.org/W2167701793","https://openalex.org/W2330018799","https://openalex.org/W2349904868","https://openalex.org/W2470014202","https://openalex.org/W2611520812","https://openalex.org/W2782303467","https://openalex.org/W2783701887","https://openalex.org/W2917928050","https://openalex.org/W2940184083","https://openalex.org/W3014367342","https://openalex.org/W3037170024","https://openalex.org/W3087188185","https://openalex.org/W3174303789","https://openalex.org/W4310122195","https://openalex.org/W4382724364","https://openalex.org/W4401363513","https://openalex.org/W4406389603","https://openalex.org/W4406619497"],"related_works":["https://openalex.org/W2091053076","https://openalex.org/W2276135356","https://openalex.org/W2330018799","https://openalex.org/W2097893893","https://openalex.org/W4388912770","https://openalex.org/W2081695658","https://openalex.org/W1967385260","https://openalex.org/W2013808532","https://openalex.org/W6887401680","https://openalex.org/W2241722169"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2026-08-27T00:00:00"}