{"id":"https://openalex.org/W7204239558","doi":"https://doi.org/10.64898/2026.08.21.746199","title":"Glycogen Synthase Kinase-3\u03b2 Regulates Cellular Prion Protein Levels","display_name":"Glycogen Synthase Kinase-3\u03b2 Regulates Cellular Prion Protein Levels","publication_year":2026,"publication_date":"2026-08-25","ids":{"openalex":"https://openalex.org/W7204239558","doi":"https://doi.org/10.64898/2026.08.21.746199"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.21.746199","doi":"https://doi.org/10.64898/2026.08.21.746199","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.21.746199","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/25/2026.08.21.746199.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/25/2026.08.21.746199.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039677303","display_name":"Kathryn S. Beauchemin","orcid":"https://orcid.org/0000-0003-2748-4423","observed_orcids":["https://orcid.org/0000-0003-2748-4423"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kathryn S. Beauchemin","raw_affiliation_strings":["Departments of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067737318","display_name":"Anna M. Schmoker","orcid":"https://orcid.org/0000-0003-2830-5650","observed_orcids":["https://orcid.org/0000-0003-2830-5650"]},"institutions":[{"id":"https://openalex.org/I4390039367","display_name":"Dartmouth Cancer Center","ror":"https://ror.org/044b05b34","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039367"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anna M. Schmoker","raw_affiliation_strings":["Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA"],"raw_orcid":"https://orcid.org/0000-0003-2830-5650","affiliations":[{"raw_affiliation_string":"Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA","institution_ids":["https://openalex.org/I4390039367"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009077284","display_name":"Joel C. Watts","orcid":"https://orcid.org/0000-0002-2604-2925","observed_orcids":["https://orcid.org/0000-0002-2604-2925"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Joel C. Watts","raw_affiliation_strings":["Tanz Centre for Research in Neurodegenerative Diseases and Department of Biochemistry, University of Toronto, Toronto, ON, Canada"],"raw_orcid":"https://orcid.org/0000-0002-2604-2925","affiliations":[{"raw_affiliation_string":"Tanz Centre for Research in Neurodegenerative Diseases and Department of Biochemistry, University of Toronto, Toronto, ON, Canada","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016987747","display_name":"Surachai Supattapone","orcid":"https://orcid.org/0000-0001-9060-4550","observed_orcids":["https://orcid.org/0000-0001-9060-4550"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Surachai Supattapone","raw_affiliation_strings":["Departments of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA","Departments of Medicine, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA"],"raw_orcid":"https://orcid.org/0000-0001-9060-4550","affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA","institution_ids":["https://openalex.org/I107672454"]},{"raw_affiliation_string":"Departments of Medicine, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5016987747"],"corresponding_institution_ids":["https://openalex.org/I107672454"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10855","display_name":"Amyotrophic Lateral Sclerosis Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycogen-synthase-kinase-3beta","display_name":"glycogen synthase kinase-3\u03b2","score":0.9779999852180481},{"id":"https://openalex.org/keywords/cellular-prion-protein","display_name":"cellular prion protein","score":0.9829999804496765},{"id":"https://openalex.org/keywords/prion-disease","display_name":"prion disease","score":0.8140000104904175},{"id":"https://openalex.org/keywords/gsk-3-inhibitors","display_name":"GSK-3 inhibitors","score":0.7720000147819519},{"id":"https://openalex.org/keywords/post-translational-regulation","display_name":"post-translational regulation","score":0.902999997138977},{"id":"https://openalex.org/keywords/prion-aggregation","display_name":"prion aggregation","score":0.5239999890327454},{"id":"https://openalex.org/keywords/prion-strains","display_name":"prion strains","score":0.6159999966621399},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.6549999713897705},{"id":"https://openalex.org/keywords/therapeutic-target","display_name":"therapeutic target","score":0.49000000953674316}],"concepts":[{"id":"https://openalex.org/C2778128677","wikidata":"https://www.wikidata.org/wiki/Q412895","display_name":"Cycloheximide","level":3,"score":0.7487000226974487},{"id":"https://openalex.org/C3019804061","wikidata":"https://www.wikidata.org/wiki/Q14881255","display_name":"Prion protein","level":3,"score":0.6762999892234802},{"id":"https://openalex.org/C104629339","wikidata":"https://www.wikidata.org/wiki/Q18258585","display_name":"GSK3B","level":4,"score":0.6288999915122986},{"id":"https://openalex.org/C182704531","wikidata":"https://www.wikidata.org/wiki/Q1364740","display_name":"Knockout mouse","level":3,"score":0.6136000156402588},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.5771999955177307},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.5651000142097473},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5516999959945679},{"id":"https://openalex.org/C192118531","wikidata":"https://www.wikidata.org/wiki/Q86360001","display_name":"Glycogen synthase","level":3,"score":0.48829999566078186},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4440999925136566},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43860000371932983},{"id":"https://openalex.org/C2777499176","wikidata":"https://www.wikidata.org/wiki/Q174936","display_name":"Glycogen","level":2,"score":0.42559999227523804},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42089998722076416},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4090000092983246},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.37529999017715454},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.35109999775886536},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.34700000286102295},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.3467000126838684},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.33629998564720154},{"id":"https://openalex.org/C77957584","wikidata":"https://www.wikidata.org/wiki/Q1133550","display_name":"Gene knockout","level":3,"score":0.33000001311302185},{"id":"https://openalex.org/C2780216420","wikidata":"https://www.wikidata.org/wiki/Q83330","display_name":"Lysosome","level":3,"score":0.32760000228881836},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.32600000500679016},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31209999322891235},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.30959999561309814},{"id":"https://openalex.org/C182996813","wikidata":"https://www.wikidata.org/wiki/Q910847","display_name":"GSK-3","level":3,"score":0.2896000146865845},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.28439998626708984},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.27090001106262207},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.26660001277923584},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.257999986410141},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.25609999895095825},{"id":"https://openalex.org/C2777066959","wikidata":"https://www.wikidata.org/wiki/Q869711","display_name":"Cricetulus","level":4,"score":0.2551000118255615}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.21.746199","doi":"https://doi.org/10.64898/2026.08.21.746199","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.21.746199","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/25/2026.08.21.746199.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.21.746199","doi":"https://doi.org/10.64898/2026.08.21.746199","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.21.746199","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/08/25/2026.08.21.746199.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","score":0.4898357689380646,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204239558.pdf","grobid_xml":"https://content.openalex.org/works/W7204239558.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2138224924","https://openalex.org/W2997957760","https://openalex.org/W2008423608","https://openalex.org/W3163675851","https://openalex.org/W2062325829","https://openalex.org/W1935457268","https://openalex.org/W2045181512","https://openalex.org/W1986941385","https://openalex.org/W4402809233","https://openalex.org/W300713757"],"abstract_inverted_index":{"ABSTRACT":[0],"The":[1],"normal":[2],"cellular":[3],"prion":[4,17,192],"protein":[5,106],"(PrP":[6],"C":[7,38,53,71,98,131,147,167,203],")":[8,83],"is":[9,30],"an":[10],"essential":[11],"substrate":[12],"in":[13,25,55,113,133,141,155,184,205],"all":[14],"forms":[15],"of":[16,51,108],"diseases":[18],"and":[19,68,127,136,143,159,213],"a":[20,48,56,170,209],"receptor":[21],"for":[22],"A\u03b2":[23],"oligomers":[24],"Alzheimer\u2019s":[26],"disease.":[27],"However,":[28],"it":[29],"not":[31,81],"fully":[32],"understood":[33],"how":[34],"cells":[35,186],"regulate":[36],"PrP":[37,52,70,97,130,146,166,181,202],"levels.":[39],"Recently,":[40],"we":[41,62],"identified":[42],"glycogen":[43],"synthase":[44],"kinase-3\u03b2":[45],"(GSK-3\u03b2)":[46],"as":[47],"potential":[49],"regulator":[50],"levels":[54,72,132,148,168,183,204],"whole":[57],"genome":[58],"knockout":[59,115,158],"screen.":[60],"Here,":[61],"show":[63],"that":[64,96,163,198],"both":[65,140,156],"cell":[66,91],"surface":[67],"total":[69,110],"can":[73],"be":[74],"reduced":[75,105,129,151,180],"either":[76,176],"by":[77,86,152],"siRNA-mediated":[78],"Gsk3b":[79,114,157],"(but":[80],"Gsk3a":[82],"knockdown":[84],"or":[85,178],"CRISPR-mediated":[87],"Gs3b":[88],"knockout.":[89],"Whole":[90],"mass":[92],"spectrometric":[93],"analysis":[94],"showed":[95],"was":[99],"the":[100,195],"60":[101],"th":[102],"most":[103],"significantly":[104],"(out":[107],"7227":[109],"proteins":[111],"detected)":[112],"cells,":[116,139,161,207],"compared":[117],"to":[118],"wild-type":[119],"cells.":[120],"Two":[121],"different":[122,190],"GSK-3":[123],"inhibitors,":[124],"laduviglusib":[125,177],"(CHIR-99021)":[126],"AZD-1080,":[128],"mouse":[134],"CAD5":[135,185],"human":[137],"BE(2)-C":[138],"undifferentiated":[142],"differentiated":[144],"states.":[145],"were":[149],"similarly":[150],"cycloheximide":[153],"treatment":[154,174],"WT":[160],"indicating":[162],"GSK-3\u03b2":[164,199],"regulates":[165],"through":[169],"post-translational":[171],"mechanism.":[172],"Finally,":[173],"with":[175,188],"AZD-1080":[179],"Sc":[182],"infected":[187],"three":[189],"rodent":[191],"strains.":[193],"Overall,":[194],"results":[196],"reveal":[197],"activity":[200],"controls":[201],"living":[206],"revealing":[208],"novel":[210],"regulatory":[211],"mechanism":[212],"promising":[214],"therapeutic":[215],"target.":[216]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-08-27T00:00:00"}