{"id":"https://openalex.org/W7204207581","doi":"https://doi.org/10.21203/rs.3.rs-10627119/v1","title":"The pVS1-VIR2 helper plasmid reduces regeneration efficiency of GRF-GIF in hexaploid wheat to obtain tri-allelic TaDOG1L4 mutations with reduced seed dormancy","display_name":"The pVS1-VIR2 helper plasmid reduces regeneration efficiency of GRF-GIF in hexaploid wheat to obtain tri-allelic TaDOG1L4 mutations with reduced seed dormancy","publication_year":2026,"publication_date":"2026-08-25","ids":{"openalex":"https://openalex.org/W7204207581","doi":"https://doi.org/10.21203/rs.3.rs-10627119/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-10627119/v1","doi":"https://doi.org/10.21203/rs.3.rs-10627119/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10627119/v1","pdf_url":"https://www.researchsquare.com/article/rs-10627119/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-10627119/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5126761755","display_name":"Renqiang Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Renqiang Li","raw_affiliation_strings":["Katholieke Universiteit Leuven"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011968389","display_name":"Muhammad Usama Hameed","orcid":"https://orcid.org/0000-0002-1943-4381","observed_orcids":["https://orcid.org/0000-0002-1943-4381"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Muhammad Usama Hameed","raw_affiliation_strings":["Katholieke Universiteit Leuven"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148071992","display_name":"Vincent Morren","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Vincent Morren","raw_affiliation_strings":["Katholieke Universiteit Leuven"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136117505","display_name":"Eduardo\u00a0A. Zelada\u00a0Lau","orcid":"https://orcid.org/0000-0002-0054-1590","observed_orcids":["https://orcid.org/0000-0002-0054-1590"]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Eduardo Andre Zelada Lau","raw_affiliation_strings":["Katholieke Universiteit Leuven"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5138798927","display_name":"Koen Geuten","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE"],"is_corresponding":true,"raw_author_name":"Koen Geuten","raw_affiliation_strings":["Katholieke Universiteit Leuven"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Katholieke Universiteit Leuven","institution_ids":["https://openalex.org/I99464096"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5138798927"],"corresponding_institution_ids":["https://openalex.org/I99464096"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.33889999985694885,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.33889999985694885,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.3328000009059906,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11098","display_name":"Seed Germination and Physiology","score":0.11739999800920486,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/seed-dormancy","display_name":"seed dormancy","score":0.8140000104904175},{"id":"https://openalex.org/keywords/pre-harvest-sprouting","display_name":"pre-harvest sprouting","score":0.8270000219345093},{"id":"https://openalex.org/keywords/hexaploid-wheat","display_name":"hexaploid wheat","score":0.8619999885559082},{"id":"https://openalex.org/keywords/shoot-regeneration","display_name":"shoot regeneration","score":0.6759999990463257},{"id":"https://openalex.org/keywords/crispr-cas9-mutagenesis","display_name":"CRISPR/Cas9 mutagenesis","score":0.871999979019165},{"id":"https://openalex.org/keywords/temperature-effects-on-dormancy","display_name":"temperature effects on dormancy","score":0.5709999799728394}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8657000064849854},{"id":"https://openalex.org/C3527866","wikidata":"https://www.wikidata.org/wiki/Q162267","display_name":"Dormancy","level":3,"score":0.8043000102043152},{"id":"https://openalex.org/C88548481","wikidata":"https://www.wikidata.org/wiki/Q2397491","display_name":"Seed dormancy","level":4,"score":0.6880999803543091},{"id":"https://openalex.org/C100701293","wikidata":"https://www.wikidata.org/wiki/Q193838","display_name":"Germination","level":2,"score":0.6811000108718872},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.6067000031471252},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.516700029373169},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.44290000200271606},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.4066999852657318},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.4011000096797943},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.392300009727478},{"id":"https://openalex.org/C30278631","wikidata":"https://www.wikidata.org/wiki/Q281721","display_name":"Chimera (genetics)","level":3,"score":0.3846000134944916},{"id":"https://openalex.org/C137580998","wikidata":"https://www.wikidata.org/wiki/Q235352","display_name":"Crop","level":2,"score":0.3774000108242035},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3582000136375427},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3321000039577484},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.33160001039505005},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.2971999943256378},{"id":"https://openalex.org/C46757340","wikidata":"https://www.wikidata.org/wiki/Q43238","display_name":"Poaceae","level":2,"score":0.27970001101493835},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.26969999074935913},{"id":"https://openalex.org/C171056886","wikidata":"https://www.wikidata.org/wiki/Q193119","display_name":"Regeneration (biology)","level":2,"score":0.25859999656677246}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-10627119/v1","doi":"https://doi.org/10.21203/rs.3.rs-10627119/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10627119/v1","pdf_url":"https://www.researchsquare.com/article/rs-10627119/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-10627119/v1","doi":"https://doi.org/10.21203/rs.3.rs-10627119/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10627119/v1","pdf_url":"https://www.researchsquare.com/article/rs-10627119/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.9208999872207642,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8145991601","display_name":null,"funder_award_id":"C24E/21/004","funder_id":"https://openalex.org/F4320322308","funder_display_name":"KU Leuven"}],"funders":[{"id":"https://openalex.org/F4320322308","display_name":"KU Leuven","ror":"https://ror.org/05f950310"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204207581.pdf","grobid_xml":"https://content.openalex.org/works/W7204207581.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W7221767003"],"related_works":["https://openalex.org/W2054472099","https://openalex.org/W2007100283","https://openalex.org/W2073723809","https://openalex.org/W1840230766","https://openalex.org/W4410739647","https://openalex.org/W2965444750","https://openalex.org/W2070934926","https://openalex.org/W7118014285","https://openalex.org/W2020764001","https://openalex.org/W3006368655"],"abstract_inverted_index":{"Abstract":[0],"Wheat":[1],"plays":[2],"important":[3],"role":[4,81,93,148],"as":[5,70,152],"a":[6,34],"staple":[7],"crop":[8],"and":[9,41,159],"in":[10,77,82,96,119,149,173],"the":[11,24,45,54,71,92,112,115,123,131,136],"supply":[12],"of":[13,26,37,56,94,114,140,171],"brewing":[14],"ingredients,":[15],"but":[16],"its":[17,42,80,146],"yield":[18],"has":[19,50],"been":[20,51],"increasingly":[21],"threatened":[22],"by":[23,53,103],"occurrence":[25],"pre-harvest":[27],"sprouting.":[28],"While":[29],"resistance":[30],"breeding":[31],"relies":[32],"on":[33],"thorough":[35],"understanding":[36],"seed":[38,73,98,150,175],"dormancy":[39,74,86,99],"genetics":[40],"interaction":[43],"with":[44,58,130,135],"environment,":[46],"obtaining":[47],"such":[48],"knowledge":[49],"hampered":[52],"difficulty":[55],"working":[57],"wheat":[59,97,106],"directly.":[60],"DELAY":[61],"OF":[62],"GERMINATION":[63],"1":[64],"was":[65],"cloned":[66],"two":[67],"decades":[68],"ago":[69],"first":[72],"specific":[75],"gene":[76],"Arabidopsis.":[78],"However,":[79],"regulating":[83,174],"cereal":[84],"grain":[85],"remains":[87],"elusive.":[88],"This":[89],"study":[90],"investigates":[91],"TaDOG1L4":[95,141],"using":[100,108],"mutants":[101],"generated":[102],"CRISPR-Cas9.":[104],"Efficient":[105],"transformation":[107,132],"immature":[109],"embryos":[110],"suggests":[111],"usefulness":[113],"GRF4-GIF1":[116,137],"protein":[117],"chimera":[118],"shoot":[120],"regeneration,":[121],"while":[122],"pVS1-VIR2":[124],"helper":[125],"plasmid":[126],"seems":[127],"to":[128],"interfere":[129],"when":[133],"combined":[134],"vector.":[138],"Phenotyping":[139],"tri-allelic":[142],"transgenic":[143],"plants":[144],"revealed":[145],"positive":[147],"dormancy,":[151],"mutant":[153],"seeds":[154],"matured":[155],"under":[156],"both":[157],"cool":[158],"warm":[160],"temperature":[161],"conditions":[162],"showed":[163],"reduced":[164],"dormancy.":[165,176],"These":[166],"results":[167],"suggest":[168],"functional":[169],"conservation":[170],"DOG1":[172]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-08-26T00:00:00"}