{"id":"https://openalex.org/W7204200149","doi":"https://doi.org/10.1016/j.xpro.2026.104790","title":"Protocol to identify genes required for cardiomyocyte development using Perturb-Seq","display_name":"Protocol to identify genes required for cardiomyocyte development using Perturb-Seq","publication_year":2026,"publication_date":"2026-08-25","ids":{"openalex":"https://openalex.org/W7204200149","doi":"https://doi.org/10.1016/j.xpro.2026.104790","pmid":"https://pubmed.ncbi.nlm.nih.gov/42647169"},"language":"en","primary_location":{"id":"doi:10.1016/j.xpro.2026.104790","doi":"https://doi.org/10.1016/j.xpro.2026.104790","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104790","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xpro.2026.104790","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013046305","display_name":"Sushama Sivakumar","orcid":"https://orcid.org/0000-0001-7877-4821","observed_orcids":["https://orcid.org/0000-0001-7877-4821"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Sushama Sivakumar","raw_affiliation_strings":["Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: sushama.sivakumar@utsouthwestern.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: sushama.sivakumar@utsouthwestern.edu","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5146628257","display_name":"Lei Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lei Wang","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070591083","display_name":"Huan Zhao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Huan Zhao","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106987584","display_name":"Mpathi Nzima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mpathi Nzima","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148098893","display_name":"David C. Park","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David C. Park","raw_affiliation_strings":["Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100428924","display_name":"Yihan Wang","orcid":"https://orcid.org/0000-0001-7440-417X","observed_orcids":["https://orcid.org/0000-0001-7440-417X"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yihan Wang","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017888430","display_name":"Anjana Sundarrajan","orcid":"https://orcid.org/0000-0003-0231-8090","observed_orcids":["https://orcid.org/0000-0003-0231-8090"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anjana Sundarrajan","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078295336","display_name":"Chikara Takeuchi","orcid":"https://orcid.org/0000-0001-6855-6580","observed_orcids":["https://orcid.org/0000-0001-6855-6580"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chikara Takeuchi","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028026254","display_name":"Minnie Deng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Minnie Deng","raw_affiliation_strings":["Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049927586","display_name":"W. Lee Kraus","orcid":"https://orcid.org/0000-0002-8786-2986","observed_orcids":["https://orcid.org/0000-0002-8786-2986"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. Lee Kraus","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073405615","display_name":"Gary C. Hon","orcid":"https://orcid.org/0000-0002-1615-0391","observed_orcids":["https://orcid.org/0000-0002-1615-0391","https://orcid.org/0000-0002-5728-4433"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gary C. Hon","raw_affiliation_strings":["Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA; Lyda Hill Department of Bioinformatics, Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: gary.hon@utsouthwestern.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA; Lyda Hill Department of Bioinformatics, Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: gary.hon@utsouthwestern.edu","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"last","author":{"id":null,"display_name":"Nikhil V. Munshi","orcid":"https://orcid.org/0000-0002-8397-100X","observed_orcids":["https://orcid.org/0000-0002-8397-100X"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Nikhil V. Munshi","raw_affiliation_strings":["Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: nikhil.munshi@utsouthwestern.edu"],"raw_orcid":"https://orcid.org/0000-0002-8397-100X","affiliations":[{"raw_affiliation_string":"Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: nikhil.munshi@utsouthwestern.edu","institution_ids":["https://openalex.org/I867280407"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013046305","https://openalex.org/A5073405615"],"corresponding_institution_ids":["https://openalex.org/I867280407"],"apc_list":{"value":1550,"currency":"USD","value_usd":1550},"apc_paid":{"value":1550,"currency":"USD","value_usd":1550},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.57092219,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":"3","first_page":"104790","last_page":"104790"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.3659999966621399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.3659999966621399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.22419999539852142,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.19200000166893005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cardiomyocyte-development","display_name":"cardiomyocyte development","score":0.9649999737739563},{"id":"https://openalex.org/keywords/perturb-seq","display_name":"Perturb-seq","score":0.9909999966621399},{"id":"https://openalex.org/keywords/crispr-screening","display_name":"CRISPR screen","score":0.7960000038146973},{"id":"https://openalex.org/keywords/single-guide-rna-library","display_name":"single guide RNA library","score":0.7630000114440918},{"id":"https://openalex.org/keywords/cardiomyocyte-differentiation","display_name":"cardiomyocyte differentiation","score":0.9120000004768372},{"id":"https://openalex.org/keywords/cell-hashing","display_name":"cell hashing","score":0.875},{"id":"https://openalex.org/keywords/single-cell-rna-sequencing","display_name":"single-cell RNA sequencing","score":0.9010000228881836},{"id":"https://openalex.org/keywords/cardiac-development-genes","display_name":"cardiac development genes","score":0.515999972820282}],"concepts":[{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.46070000529289246},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4528999924659729},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.444599986076355},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.430400013923645},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41440001130104065},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.3050000071525574},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.2572999894618988},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.25060001015663147},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2475000023841858},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.24330000579357147}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1016/j.xpro.2026.104790","doi":"https://doi.org/10.1016/j.xpro.2026.104790","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104790","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},{"id":"pmid:42647169","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42647169","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR protocols","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13543896","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13543896/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13543896/pdf/main.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"STAR Protoc","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1016/j.xpro.2026.104790","doi":"https://doi.org/10.1016/j.xpro.2026.104790","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2026.104790","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4000000059604645,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/2","score":0.489655464887619,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1232349411","display_name":"Systematic Assessment of Combinatorial Transcription Factor Activity","funder_award_id":"1r56hg012768-01a1","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G390011392","display_name":null,"funder_award_id":"UM1HG011996","funder_id":"https://openalex.org/F4320306078","funder_display_name":"U.S. Department of Defense"},{"id":"https://openalex.org/G5040387946","display_name":"Comprehensive Evaluation of Functional Enhancers in Breast Cancer Risk Susceptibility Loci","funder_award_id":"RP190451","funder_id":"https://openalex.org/F4320308129","funder_display_name":"Cancer Prevention and Research Institute of Texas"},{"id":"https://openalex.org/G5085406572","display_name":"Combinatorial Biology of Gene Regulation for Cellular Engineering","funder_award_id":"1dp2gm128203-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6245228462","display_name":null,"funder_award_id":"PR172060","funder_id":"https://openalex.org/F4320306078","funder_display_name":"U.S. Department of Defense"},{"id":"https://openalex.org/G7296301616","display_name":"Defining regenerative potential in the cardiac conduction system","funder_award_id":"R01HL136604","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G9000881871","display_name":"Multiscale functional characterization of genomic variation in human developmental disorders","funder_award_id":"5um1hg011996-05","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000888981","display_name":"Exploring Mechanisms of Cardiac Pacemaker Cell Fate Determination","funder_award_id":"R01HL151650","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320306078","display_name":"U.S. Department of Defense","ror":"https://ror.org/0447fe631"},{"id":"https://openalex.org/F4320306133","display_name":"Burroughs Wellcome Fund","ror":"https://ror.org/01d35cw23"},{"id":"https://openalex.org/F4320308129","display_name":"Cancer Prevention and Research Institute of Texas","ror":"https://ror.org/0003xa228"},{"id":"https://openalex.org/F4320310169","display_name":"University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":3,"referenced_works":["https://openalex.org/W4313232103","https://openalex.org/W4406487146","https://openalex.org/W4416179677"],"related_works":[],"abstract_inverted_index":{"While":[0],"Perturb-Seq":[1],"combines":[2],"CRISPR-based":[3],"screening":[4],"with":[5],"single-cell":[6],"RNA":[7],"sequencing":[8],"(scRNA-seq),":[9],"large-scale":[10],"experiments":[11],"are":[12],"costly":[13],"and":[14,48,56,65,74],"its":[15],"application":[16],"during":[17],"development":[18,35],"is":[19],"complicated":[20],"by":[21],"differentiation":[22],"heterogeneity.":[23],"Here,":[24],"we":[25],"present":[26],"a":[27],"protocol":[28],"to":[29,81],"identify":[30],"genes":[31],"required":[32],"for":[33,41,62],"cardiomyocyte":[34,50],"using":[36],"Perturb-Seq.":[37],"We":[38,58],"describe":[39],"steps":[40],"sgRNA":[42],"(single":[43],"guide":[44],"RNA)":[45],"library":[46],"cloning":[47],"infection,":[49],"differentiation,":[51],"cell":[52],"hashing,":[53],"super":[54],"loading,":[55],"scRNA-seq.":[57],"then":[59],"detail":[60],"procedures":[61],"sequencing,":[63],"mapping,":[64],"data":[66],"analysis.":[67],"For":[68],"complete":[69],"details":[70],"on":[71],"the":[72],"use":[73],"execution":[75],"of":[76],"this":[77],"protocol,":[78],"please":[79],"refer":[80],"Sivakumar":[82],"et":[83],"al.":[84],"1":[85]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2026-08-26T00:00:00"}
