{"id":"https://openalex.org/W7204190495","doi":"https://doi.org/10.1016/j.slast.2026.100458","title":"A scalable high-density microwell assay for single-cell clonal expansion profiling","display_name":"A scalable high-density microwell assay for single-cell clonal expansion profiling","publication_year":2026,"publication_date":"2026-08-25","ids":{"openalex":"https://openalex.org/W7204190495","doi":"https://doi.org/10.1016/j.slast.2026.100458","pmid":"https://pubmed.ncbi.nlm.nih.gov/42641793"},"language":"en","primary_location":{"id":"doi:10.1016/j.slast.2026.100458","doi":"https://doi.org/10.1016/j.slast.2026.100458","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.slast.2026.100458","pdf_url":null,"source":{"id":"https://openalex.org/S4387277866","display_name":"SLAS TECHNOLOGY","issn_l":"2472-6303","issn":["2472-6303","2472-6311"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Technology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.slast.2026.100458","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053651577","display_name":"Karoliina Stefanius","orcid":"https://orcid.org/0000-0002-9456-1954","observed_orcids":["https://orcid.org/0000-0002-9456-1954"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Stefanius Karoliina","raw_affiliation_strings":["SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA. Electronic address: kstefanius@scellbio.com"],"raw_orcid":"https://orcid.org/0000-0002-9456-1954","affiliations":[{"raw_affiliation_string":"SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA. Electronic address: kstefanius@scellbio.com","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5133576225","display_name":"Shiska Raut","orcid":"https://orcid.org/0009-0008-7509-4984","observed_orcids":["https://orcid.org/0009-0008-7509-4984"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Raut Shiska","raw_affiliation_strings":["SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA"],"raw_orcid":"https://orcid.org/0009-0008-7509-4984","affiliations":[{"raw_affiliation_string":"SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022215890","display_name":"Bryan Presley","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Presley Bryan","raw_affiliation_strings":["SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"SingleCell Biotechnology, Pegasus Park, Biolabs, Dallas, TX, 75247, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112286340","display_name":"Digant P. Dav\u00e9","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189196454","display_name":"The University of Texas at Arlington","ror":"https://ror.org/019kgqr73","country_code":"US","type":"education","lineage":["https://openalex.org/I189196454"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Dave Digant P","raw_affiliation_strings":["Department of Bioengineering, The University of Texas at Arlington, Arlington, TX, 76010, USA. Electronic address: ddave@uta.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, The University of Texas at Arlington, Arlington, TX, 76010, USA. Electronic address: ddave@uta.edu","institution_ids":["https://openalex.org/I189196454"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5053651577","https://openalex.org/A5112286340"],"corresponding_institution_ids":["https://openalex.org/I189196454"],"apc_list":{"value":2070,"currency":"USD","value_usd":2070},"apc_paid":{"value":2070,"currency":"USD","value_usd":2070},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.60908738,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"42","issue":null,"first_page":"100458","last_page":"100458"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.9423999786376953,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.9423999786376953,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":0.024900000542402267,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microwell-arrays","display_name":"microwell arrays","score":0.7839999794960022},{"id":"https://openalex.org/keywords/clonogenic-assay","display_name":"clonogenic assay","score":0.8360000252723694},{"id":"https://openalex.org/keywords/self-renewal","display_name":"self-renewal","score":0.734000027179718},{"id":"https://openalex.org/keywords/glioblastoma","display_name":"glioblastoma","score":0.7940000295639038},{"id":"https://openalex.org/keywords/plating-efficiency","display_name":"plating efficiency","score":0.40400001406669617},{"id":"https://openalex.org/keywords/automated-image-analysis","display_name":"automated image analysis","score":0.7839999794960022},{"id":"https://openalex.org/keywords/tumor-heterogeneity","display_name":"tumor heterogeneity","score":0.5019999742507935}],"concepts":[{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5353000164031982},{"id":"https://openalex.org/C187191949","wikidata":"https://www.wikidata.org/wiki/Q1138496","display_name":"Profiling (computer programming)","level":2,"score":0.5},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4887999892234802},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3783000111579895},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.3151000142097473},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.2745000123977661},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.25519999861717224},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.25519999861717224},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.2515000104904175},{"id":"https://openalex.org/C2776401178","wikidata":"https://www.wikidata.org/wiki/Q12050496","display_name":"Feature (linguistics)","level":2,"score":0.23810000717639923}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.slast.2026.100458","doi":"https://doi.org/10.1016/j.slast.2026.100458","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.slast.2026.100458","pdf_url":null,"source":{"id":"https://openalex.org/S4387277866","display_name":"SLAS TECHNOLOGY","issn_l":"2472-6303","issn":["2472-6303","2472-6311"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Technology","raw_type":"journal-article"},{"id":"doi:10.64898/2026.04.10.717842","doi":"https://doi.org/10.64898/2026.04.10.717842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.04.10.717842","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/04/14/2026.04.10.717842.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:42641793","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42641793","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS technology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:506950611d084c4d91be9774c9014e9a","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/506950611d084c4d91be9774c9014e9a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"SLAS Technology, Vol 42, Iss , Pp 100458- (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.slast.2026.100458","doi":"https://doi.org/10.1016/j.slast.2026.100458","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.slast.2026.100458","pdf_url":null,"source":{"id":"https://openalex.org/S4387277866","display_name":"SLAS TECHNOLOGY","issn_l":"2472-6303","issn":["2472-6303","2472-6311"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SLAS Technology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4043000042438507}],"sustainable_development_goals_aurora":[{"display_name":"Affordable and clean energy","id":"https://metadata.un.org/sdg/7","score":0.5545530319213867}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7568444536","display_name":"A Novel High Throughput Platform for Drug Screening Against Dormant and Migrating High-Grade Glioma Cells","funder_award_id":"DP240117","funder_id":"https://openalex.org/F4320308129","funder_display_name":"Cancer Prevention and Research Institute of Texas"}],"funders":[{"id":"https://openalex.org/F4320308129","display_name":"Cancer Prevention and Research Institute of Texas","ror":"https://ror.org/0003xa228"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1976112178","https://openalex.org/W1981028296","https://openalex.org/W1987012261","https://openalex.org/W2000744297","https://openalex.org/W2001544973","https://openalex.org/W2003382153","https://openalex.org/W2017883800","https://openalex.org/W2017908665","https://openalex.org/W2018288777","https://openalex.org/W2033436239","https://openalex.org/W2037139632","https://openalex.org/W2040458067","https://openalex.org/W2041684667","https://openalex.org/W2044895919","https://openalex.org/W2060551076","https://openalex.org/W2078510083","https://openalex.org/W2080838269","https://openalex.org/W2100093425","https://openalex.org/W2108835820","https://openalex.org/W2132834292","https://openalex.org/W2165316896","https://openalex.org/W2178256888","https://openalex.org/W2267374750","https://openalex.org/W2791761931","https://openalex.org/W2794209007","https://openalex.org/W2805744143","https://openalex.org/W3009359263","https://openalex.org/W3096928956","https://openalex.org/W3143381404","https://openalex.org/W3170035660","https://openalex.org/W3189300114","https://openalex.org/W3209608975","https://openalex.org/W4221002170","https://openalex.org/W4223955424","https://openalex.org/W4224279511","https://openalex.org/W4225248008","https://openalex.org/W4284959126","https://openalex.org/W4308262349","https://openalex.org/W4323971871","https://openalex.org/W4386934070","https://openalex.org/W4387105196","https://openalex.org/W4390482949","https://openalex.org/W4392881437","https://openalex.org/W4393951524","https://openalex.org/W4407757961","https://openalex.org/W4411443013","https://openalex.org/W4412021061"],"related_works":["https://openalex.org/W7154733408","https://openalex.org/W2794209007","https://openalex.org/W1986203126","https://openalex.org/W403458925","https://openalex.org/W289865863","https://openalex.org/W3109765667","https://openalex.org/W1995616824","https://openalex.org/W1966203620","https://openalex.org/W2164527416","https://openalex.org/W112041751"],"abstract_inverted_index":{"Traditional":[0],"clonogenic":[1,52,175,221],"assays":[2],"remain":[3],"central":[4],"to":[5,23,34,75,133],"evaluating":[6],"the":[7,14,29,215],"self-renewal":[8],"capacity":[9,177],"of":[10,49,81,111,174,218],"tumor":[11],"cells.":[12],"However,":[13],"assay":[15,123,168],"relies":[16],"on":[17],"subjective":[18],"endpoint":[19],"measurements,":[20],"is":[21,183],"restricted":[22],"two-dimensional":[24],"monolayer":[25],"growth,":[26],"and":[27,63,99,129,136,156,182,192,203],"lacks":[28],"single":[30,50,153,179,194,219],"cell":[31,51,57,145,180,195,220],"resolution":[32,181,217],"required":[33],"resolve":[35],"heterogeneous":[36],"expansion":[37,176,202,205],"behaviors.":[38],"We":[39],"describe":[40],"a":[41,208],"high-density":[42,210],"microwell":[43,91],"array-based":[44],"platform":[45,87,169],"for":[46],"quantitative":[47,172],"assessment":[48],"growth":[53,77,159],"outcomes,":[54],"defined":[55,76],"by":[56],"count":[58],"distributions":[59,161],"spanning":[60],"non-dividing,":[61],"slow-dividing,":[62],"fast-dividing":[64],"three-dimensional":[65],"colony":[66,226],"forming":[67],"phenotypes.":[68],"This":[69,165],"approach":[70,213],"links":[71],"initial":[72],"single-cell":[73,199],"occupancy":[74,155],"outcomes":[78,206],"across":[79,138,162],"thousands":[80],"indexed":[82,113],"microwells":[83,115],"per":[84],"well.":[85],"The":[86,122],"integrates":[88],"high-density,":[89],"low-adhesion":[90],"arrays":[92],"within":[93,207],"industry":[94],"standard":[95],"device":[96],"plate":[97,131,140],"formats":[98,132],"an":[100],"automated":[101,119],"image":[102],"analysis":[103],"pipeline":[104],"incorporating":[105],"machine":[106],"learning,":[107],"enabling":[108],"parallel":[109],"quantification":[110],"spatially":[112],"founder-derived":[114],"using":[116],"widely":[117],"accessible":[118],"imaging":[120],"systems.":[121],"was":[124],"implemented":[125],"in":[126,187],"both":[127],"4-well":[128],"96-well":[130],"evaluate":[134],"reproducibility":[135],"scalability":[137],"different":[139],"configurations.":[141],"Using":[142],"three":[143],"glioblastoma":[144],"lines":[146],"as":[147],"model":[148],"systems,":[149],"we":[150],"demonstrate":[151],"reproducible":[152],"founder":[154],"consistent":[157],"clonal":[158],"outcome":[160],"replicate":[163],"formats.":[164],"integrated":[166],"microscale":[167],"enables":[170],"systematic":[171],"characterization":[173],"at":[178],"compatible":[184],"with":[185],"applications":[186],"cancer":[188],"biology,":[189],"therapeutic":[190],"testing,":[191],"functional":[193],"phenotyping.":[196],"By":[197],"resolving":[198],"persistence,":[200],"limited":[201],"high":[204],"scalable":[209],"format,":[211],"this":[212],"expands":[214],"analytical":[216],"profiling":[222],"beyond":[223],"traditional":[224],"binary":[225],"scoring.":[227]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2026-08-26T00:00:00"}
