{"id":"https://openalex.org/W7204125394","doi":"https://doi.org/10.21203/rs.3.rs-10414016/v1","title":"Water oxidation-driven histidine dioxidation enables probe-free proximity labeling","display_name":"Water oxidation-driven histidine dioxidation enables probe-free proximity labeling","publication_year":2026,"publication_date":"2026-08-24","ids":{"openalex":"https://openalex.org/W7204125394","doi":"https://doi.org/10.21203/rs.3.rs-10414016/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-10414016/v1","doi":"https://doi.org/10.21203/rs.3.rs-10414016/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10414016/v1","pdf_url":"https://www.researchsquare.com/article/rs-10414016/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-10414016/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046785997","display_name":"Tae\u2010Hyuk Kwon","orcid":"https://orcid.org/0000-0002-1633-6065","observed_orcids":["https://orcid.org/0000-0002-1633-6065"]},"institutions":[{"id":"https://openalex.org/I48566637","display_name":"Ulsan National Institute of Science and Technology","ror":"https://ror.org/017cjz748","country_code":"KR","type":"education","lineage":["https://openalex.org/I48566637"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Tae-Hyuk Kwon","raw_affiliation_strings":["Ulsan National Institute of Science and Technology"],"raw_orcid":"https://orcid.org/0000-0002-1633-6065","affiliations":[{"raw_affiliation_string":"Ulsan National Institute of Science and Technology","institution_ids":["https://openalex.org/I48566637"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089425288","display_name":"Chaiheon Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chaiheon Lee","raw_affiliation_strings":["Broad Institute of MIT and Havard"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Broad Institute of MIT and Havard","institution_ids":["https://openalex.org/I107606265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032287046","display_name":"Jeong Kyeong Lee","orcid":"https://orcid.org/0009-0001-3687-4311","observed_orcids":["https://orcid.org/0009-0001-3687-4311"]},"institutions":[{"id":"https://openalex.org/I48566637","display_name":"Ulsan National Institute of Science and Technology","ror":"https://ror.org/017cjz748","country_code":"KR","type":"education","lineage":["https://openalex.org/I48566637"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jeong Kyeong Lee","raw_affiliation_strings":["Ulsan National Institute of Science and Technology (UNIST)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulsan National Institute of Science and Technology (UNIST)","institution_ids":["https://openalex.org/I48566637"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000327490","display_name":"Chang\u2010Mo Yoo","orcid":"https://orcid.org/0000-0001-9370-2789","observed_orcids":["https://orcid.org/0000-0001-9370-2789"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Chang-Mo Yoo","raw_affiliation_strings":["Seoul National University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Seoul National University","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035687111","display_name":"Byeong Gyu KIM","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104335","display_name":"Institute for Basic Science","ror":"https://ror.org/00y0zf565","country_code":"KR","type":"facility","lineage":["https://openalex.org/I4210104335"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Byeong Gyu Kim","raw_affiliation_strings":["Institute for Basic Science"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Basic Science","institution_ids":["https://openalex.org/I4210104335"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004834270","display_name":"Gwangsu Yoon","orcid":"https://orcid.org/0000-0002-1900-6504","observed_orcids":["https://orcid.org/0000-0002-1900-6504"]},"institutions":[{"id":"https://openalex.org/I48566637","display_name":"Ulsan National Institute of Science and Technology","ror":"https://ror.org/017cjz748","country_code":"KR","type":"education","lineage":["https://openalex.org/I48566637"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Gwangsu Yoon","raw_affiliation_strings":["Ulsan National Institute of Science and Technology (UNIST)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ulsan National Institute of Science and Technology (UNIST)","institution_ids":["https://openalex.org/I48566637"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103947909","display_name":"Jiwoong Kang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jiwoong Kang","raw_affiliation_strings":["Seoul National University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Seoul National University","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088522308","display_name":"Seungjin Na","orcid":"https://orcid.org/0000-0002-5159-2048","observed_orcids":["https://orcid.org/0000-0002-5159-2048"]},"institutions":[{"id":"https://openalex.org/I4210139925","display_name":"Korea Basic Science Institute","ror":"https://ror.org/0417sdw47","country_code":"KR","type":"facility","lineage":["https://openalex.org/I2801339556","https://openalex.org/I4210139925","https://openalex.org/I4210144908","https://openalex.org/I4387152098"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Seungjin Na","raw_affiliation_strings":["Korea Basic Science Institute"],"raw_orcid":"https://orcid.org/0000-0002-5159-2048","affiliations":[{"raw_affiliation_string":"Korea Basic Science Institute","institution_ids":["https://openalex.org/I4210139925"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049147819","display_name":"Hyun\u2010Woo Rhee","orcid":"https://orcid.org/0000-0002-3817-3455","observed_orcids":["https://orcid.org/0000-0002-3817-3455"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Hyun-Woo Rhee","raw_affiliation_strings":["Seoul National University, Korea"],"raw_orcid":"https://orcid.org/0000-0002-3817-3455","affiliations":[{"raw_affiliation_string":"Seoul National University, Korea","institution_ids":["https://openalex.org/I139264467"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5046785997"],"corresponding_institution_ids":["https://openalex.org/I48566637"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/water-oxidation","display_name":"water oxidation","score":0.7300000190734863},{"id":"https://openalex.org/keywords/hydroxyl-radicals","display_name":"hydroxyl radicals","score":0.8080000281333923},{"id":"https://openalex.org/keywords/superoxide-radicals","display_name":"superoxide","score":0.996999979019165},{"id":"https://openalex.org/keywords/spatial-proteomics","display_name":"spatial proteomics","score":0.7730000019073486},{"id":"https://openalex.org/keywords/exosomes","display_name":"exosomes","score":0.453000009059906},{"id":"https://openalex.org/keywords/endosomes","display_name":"endosomes","score":0.8809999823570251},{"id":"https://openalex.org/keywords/vesicular-trafficking","display_name":"vesicle trafficking","score":0.8679999709129333},{"id":"https://openalex.org/keywords/spatial-bias","display_name":"spatial bias","score":0.4399999976158142}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7174999713897705},{"id":"https://openalex.org/C2778460671","wikidata":"https://www.wikidata.org/wiki/Q485277","display_name":"Histidine","level":3,"score":0.6488999724388123},{"id":"https://openalex.org/C2777516457","wikidata":"https://www.wikidata.org/wiki/Q2413613","display_name":"Singlet oxygen","level":3,"score":0.6334999799728394},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.5306000113487244},{"id":"https://openalex.org/C930959","wikidata":"https://www.wikidata.org/wiki/Q4086920","display_name":"Bioorthogonal chemistry","level":3,"score":0.42399999499320984},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.3930000066757202},{"id":"https://openalex.org/C54582936","wikidata":"https://www.wikidata.org/wiki/Q16814","display_name":"Photodissociation","level":2,"score":0.38839998841285706},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.3822000026702881},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.37389999628067017},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3573000133037567},{"id":"https://openalex.org/C139066938","wikidata":"https://www.wikidata.org/wiki/Q185056","display_name":"Radical","level":2,"score":0.3395000100135803},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3190000057220459},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.3165000081062317},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3109000027179718},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.295199990272522},{"id":"https://openalex.org/C57600042","wikidata":"https://www.wikidata.org/wiki/Q227564","display_name":"Oxidative phosphorylation","level":2,"score":0.29260000586509705},{"id":"https://openalex.org/C68289359","wikidata":"https://www.wikidata.org/wiki/Q7502514","display_name":"Shotgun proteomics","level":4,"score":0.28780001401901245},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.28540000319480896},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.2851000130176544},{"id":"https://openalex.org/C65165184","wikidata":"https://www.wikidata.org/wiki/Q218831","display_name":"Photocatalysis","level":3,"score":0.27489998936653137},{"id":"https://openalex.org/C175969161","wikidata":"https://www.wikidata.org/wiki/Q323426","display_name":"Exocytosis","level":3,"score":0.2653999924659729}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-10414016/v1","doi":"https://doi.org/10.21203/rs.3.rs-10414016/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10414016/v1","pdf_url":"https://www.researchsquare.com/article/rs-10414016/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-10414016/v1","doi":"https://doi.org/10.21203/rs.3.rs-10414016/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-10414016/v1","pdf_url":"https://www.researchsquare.com/article/rs-10414016/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7204125394.pdf","grobid_xml":"https://content.openalex.org/works/W7204125394.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7162652427","https://openalex.org/W4308184992","https://openalex.org/W7202281046","https://openalex.org/W3193294766","https://openalex.org/W7223093502","https://openalex.org/W7207540590","https://openalex.org/W7161127017","https://openalex.org/W7162790802","https://openalex.org/W3208579898","https://openalex.org/W7164355882"],"abstract_inverted_index":{"Abstract":[0],"Photocatalytic":[1],"proximity":[2],"labeling":[3],"(photo-PL)":[4],"has":[5],"emerged":[6],"as":[7,61,129],"a":[8,99,120,171,183,194],"powerful":[9],"tool":[10],"for":[11,103,199],"spatial":[12,51,152,200],"proteomics":[13],"in":[14,55,108,203],"subcellular":[15],"compartments.":[16],"However,":[17],"many":[18],"photo-PL":[19],"toolboxes":[20],"rely":[21],"on":[22],"singlet":[23],"oxygen":[24],"(":[25],"1":[26,94],"O":[27,95],"2":[28,85,96],")":[29,87],"to":[30,161],"generate":[31],"highly":[32],"unstable":[33],"endoperoxide":[34],"intermediates":[35],"that":[36,78,125,167,188],"must":[37],"be":[38],"trapped":[39],"immediately":[40],"by":[41],"high":[42],"concentrations":[43],"of":[44,93],"exogenous":[45],"probes.":[46],"This":[47],"constraint":[48],"can":[49,150],"bias":[50,153],"proteome":[52],"coverage,":[53],"particularly":[54],"dynamic":[56],"and":[57,63,82,135,170],"heterogeneous":[58,156],"compartments":[59],"such":[60],"endosomes":[62],"exosomes,":[64],"where":[65],"probe":[66,147,157],"accessibility":[67],"is":[68,126],"intrinsically":[69],"nonuniform.":[70],"Here,":[71],"we":[72,165,192],"develop":[73],"IDM,":[74],"an":[75],"organic":[76],"photocatalyst":[77],"generates":[79],"hydroxyl":[80],"(\u2022OH)":[81],"superoxide":[83],"(O":[84],"\u2022\u2212":[86],"radicals":[88,114],"via":[89],"water":[90],"oxidation":[91,144],"instead":[92],",":[97],"enabling":[98],"synergistic":[100],"dual-radical":[101],"mechanism":[102],"probe-free":[104],"proximal":[105,116],"protein":[106,201],"mapping":[107,202],"live":[109],"cells":[110],"(PF-Map).":[111],"The":[112],"resulting":[113],"dioxidize":[115],"histidine":[117,143],"residues":[118],"into":[119],"persistent":[121],"dioxidized":[122],"state":[123],"(His-2O)":[124],"thermodynamically":[127],"stabilized":[128],"lactam":[130],"tautomers,":[131],"which":[132],"remain":[133],"electrophilic":[134],"chemically":[136],"addressable":[137],"after":[138],"cell":[139],"lysis.":[140],"By":[141],"decoupling":[142],"from":[145,155],"live\u2011cell":[146],"capture,":[148],"PF\u2011Map":[149,160,169,180],"minimize":[151],"arising":[154],"distribution.":[158],"Applying":[159],"intracellular":[162],"vesicle":[163,185],"trafficking,":[164],"find":[166],"both":[168],"probe\u2011dependent":[172],"workflow":[173],"(PD\u2011Map)":[174],"robustly":[175],"identify":[176],"exosome":[177],"markers,":[178],"whereas":[179],"additionally":[181],"reveals":[182],"hidden":[184],"trafficking\u2013related":[186],"subproteome":[187],"PD-Map":[189],"underestimated.":[190],"Together,":[191],"establish":[193],"minimally":[195],"biased":[196],"photocatalytic":[197],"strategy":[198],"complex":[204],"biological":[205],"systems.":[206]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-08-25T00:00:00"}