{"id":"https://openalex.org/W7204117987","doi":"https://doi.org/10.1007/978-1-0716-5162-9_5","title":"CRISPR/Cas-Mediated Genome Editing for Developing Herbicide Tolerant Rice: A Step-by-Step Protocol","display_name":"CRISPR/Cas-Mediated Genome Editing for Developing Herbicide Tolerant Rice: A Step-by-Step Protocol","publication_year":2026,"publication_date":"2026-01-01","ids":{"openalex":"https://openalex.org/W7204117987","doi":"https://doi.org/10.1007/978-1-0716-5162-9_5","pmid":"https://pubmed.ncbi.nlm.nih.gov/42638006"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-0716-5162-9_5","doi":"https://doi.org/10.1007/978-1-0716-5162-9_5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-0716-5162-9_5","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W7204084020","display_name":"Plant Biotic Stress"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113970392","display_name":"Dasari Sreekanth","orcid":"https://orcid.org/0000-0001-9936-1006","observed_orcids":["https://orcid.org/0000-0001-9936-1006"]},"institutions":[{"id":"https://openalex.org/I179420787","display_name":"Indian Council of Agricultural Research","ror":"https://ror.org/04fw54a43","country_code":"IN","type":"government","lineage":["https://openalex.org/I179420787"]},{"id":"https://openalex.org/I4210131234","display_name":"Directorate of Weed Research","ror":"https://ror.org/02hzb7188","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Dasari Sreekanth","raw_affiliation_strings":["ICAR- Directorate of Weed Research, Jabalpur, 482 004, India. sreekanthplantsciences@gmail.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- Directorate of Weed Research, Jabalpur, 482 004, India. sreekanthplantsciences@gmail.com","institution_ids":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136061247","display_name":"Chandrakant Singh","orcid":"https://orcid.org/0000-0002-4573-6279","observed_orcids":["https://orcid.org/0000-0002-4573-6279"]},"institutions":[{"id":"https://openalex.org/I4210131234","display_name":"Directorate of Weed Research","ror":"https://ror.org/02hzb7188","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Chandrakant Singh","raw_affiliation_strings":["ICAR- Directorate of Weed Research, Jabalpur, 482 004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- Directorate of Weed Research, Jabalpur, 482 004, India","institution_ids":["https://openalex.org/I4210131234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113043289","display_name":"Deepak Vishwanath Pawar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131234","display_name":"Directorate of Weed Research","ror":"https://ror.org/02hzb7188","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Deepak Vishwanath Pawar","raw_affiliation_strings":["ICAR- Directorate of Weed Research, Jabalpur, 482 004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- Directorate of Weed Research, Jabalpur, 482 004, India","institution_ids":["https://openalex.org/I4210131234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5134222853","display_name":"MANOJ KUMAR YADAV","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131234","display_name":"Directorate of Weed Research","ror":"https://ror.org/02hzb7188","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Manoj Kumar Yadav","raw_affiliation_strings":["ICAR- Directorate of Weed Research, Jabalpur, 482 004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- Directorate of Weed Research, Jabalpur, 482 004, India","institution_ids":["https://openalex.org/I4210131234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5117455641","display_name":"I. K. Sahadeo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131234","display_name":"Directorate of Weed Research","ror":"https://ror.org/02hzb7188","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210131234"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"I. K. Sahadeo","raw_affiliation_strings":["ICAR- Directorate of Weed Research, Jabalpur, 482 004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- Directorate of Weed Research, Jabalpur, 482 004, India","institution_ids":["https://openalex.org/I4210131234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050829476","display_name":"Patil Siddanagouda Basavaraj","orcid":"https://orcid.org/0000-0001-6071-8987","observed_orcids":["https://orcid.org/0000-0001-6071-8987"]},"institutions":[{"id":"https://openalex.org/I4210166936","display_name":"National Institute of Abiotic Stress Management","ror":"https://ror.org/05h9t7c44","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210166936"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"P. S. Basavaraj","raw_affiliation_strings":["ICAR- National Institute of Abiotic Stress Management, Baramati, 413 115, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR- National Institute of Abiotic Stress Management, Baramati, 413 115, India","institution_ids":["https://openalex.org/I4210166936"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5147963476","display_name":"Rajeev Kumar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210126648","display_name":"Indian Institute of Vegetable Research","ror":"https://ror.org/032kjn442","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210126648"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajeev Kumar","raw_affiliation_strings":["ICAR-Indian Institute of Vegetable Research, Varanasi, 221 305, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR-Indian Institute of Vegetable Research, Varanasi, 221 305, India","institution_ids":["https://openalex.org/I4210126648"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104316677","display_name":"Survi Mahesh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Survi Mahesh","raw_affiliation_strings":["Louvain Institute of Biomolecular Science and Technology, Universit\u00e9 Catholique de Louvain, Louvain-La-Neuve, 1348, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Louvain Institute of Biomolecular Science and Technology, Universit\u00e9 Catholique de Louvain, Louvain-La-Neuve, 1348, Belgium","institution_ids":["https://openalex.org/I95674353"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5113970392"],"corresponding_institution_ids":["https://openalex.org/I179420787","https://openalex.org/I4210131234"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"3017","issue":null,"first_page":"47","last_page":"57"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10774","display_name":"Weed Control and Herbicide Applications","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.008799999952316284,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas-genome-editing","display_name":"CRISPR/Cas genome editing","score":0.9879999756813049},{"id":"https://openalex.org/keywords/bispyribac-sodium","display_name":"bispyribac-sodium","score":0.8640000224113464},{"id":"https://openalex.org/keywords/acetolactate-synthase","display_name":"acetolactate synthase","score":0.9430000185966492},{"id":"https://openalex.org/keywords/als-gene","display_name":"ALS gene","score":0.5230000019073486},{"id":"https://openalex.org/keywords/herbicide-resistance","display_name":"herbicide resistance","score":0.8840000033378601},{"id":"https://openalex.org/keywords/echinochloa","display_name":"Echinochloa","score":0.8600000143051147},{"id":"https://openalex.org/keywords/weed-control","display_name":"weed control","score":0.6710000038146973},{"id":"https://openalex.org/keywords/guide-rna-design","display_name":"guide RNA design","score":0.8339999914169312},{"id":"https://openalex.org/keywords/targeted-mutagenesis","display_name":"targeted mutagenesis","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.5882999897003174},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5533000230789185},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4975000023841858},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4927000105381012},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49230000376701355},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.487199991941452},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3068999946117401},{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.2574999928474426},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.22110000252723694},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22100000083446503}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000095","descriptor_name":"Acetolactate Synthase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001565","descriptor_name":"Benzoates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006540","descriptor_name":"Herbicides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D011743","descriptor_name":"Pyrimidines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D053209","descriptor_name":"Herbicide Resistance","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-0716-5162-9_5","doi":"https://doi.org/10.1007/978-1-0716-5162-9_5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-0716-5162-9_5","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:42638006","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42638006","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8409000039100647,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1262867747","https://openalex.org/W1992783297","https://openalex.org/W2010851273","https://openalex.org/W2024556021","https://openalex.org/W2035420256","https://openalex.org/W4200092326","https://openalex.org/W4300724666","https://openalex.org/W4388526993","https://openalex.org/W4403005605","https://openalex.org/W4410136378"],"related_works":["https://openalex.org/W3044345033","https://openalex.org/W7155176604","https://openalex.org/W4392967644","https://openalex.org/W4403353047","https://openalex.org/W4414779981","https://openalex.org/W4413232209","https://openalex.org/W3102748721","https://openalex.org/W4414560878","https://openalex.org/W4315798426","https://openalex.org/W4408830153"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2026-08-25T00:00:00"}