{"id":"https://openalex.org/W7203887353","doi":"https://doi.org/10.3791/71394","title":"Optogenetic Analysis of Behavior in the Mosquito Aedes aegypti","display_name":"Optogenetic Analysis of Behavior in the Mosquito Aedes aegypti","publication_year":2026,"publication_date":"2026-08-21","ids":{"openalex":"https://openalex.org/W7203887353","doi":"https://doi.org/10.3791/71394","pmid":"https://pubmed.ncbi.nlm.nih.gov/41890001","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13015328"},"language":"en","primary_location":{"id":"doi:10.3791/71394","doi":"https://doi.org/10.3791/71394","is_oa":false,"landing_page_url":"https://doi.org/10.3791/71394","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/18/2026.03.15.711871.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5118580365","display_name":"Spruha Rami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Spruha Rami","raw_affiliation_strings":["Department of Genetics, Yale University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Yale University","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077530985","display_name":"Michelle So","orcid":"https://orcid.org/0000-0001-5411-3711","observed_orcids":["https://orcid.org/0000-0001-5411-3711"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle So","raw_affiliation_strings":["Department of Genetics, Yale University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Yale University","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130126524","display_name":"Cassandra Travis","orcid":"https://orcid.org/0009-0009-5989-6298","observed_orcids":["https://orcid.org/0009-0009-5989-6298"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cassandra Travis","raw_affiliation_strings":["Department of Genetics, Yale University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Yale University","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007655632","display_name":"Yaoyu Jiao","orcid":"https://orcid.org/0000-0002-0090-3328","observed_orcids":["https://orcid.org/0000-0002-0090-3328"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yaoyu Jiao","raw_affiliation_strings":["Department of Genetics, Yale University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Yale University","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076782921","display_name":"Paul S. Shamble","orcid":"https://orcid.org/0000-0003-4639-9339","observed_orcids":["https://orcid.org/0000-0003-4639-9339"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul Shamble","raw_affiliation_strings":["Kavli Institute for Neuroscience, Yale University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kavli Institute for Neuroscience, Yale University","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074556313","display_name":"Trevor R. Sorrells","orcid":"https://orcid.org/0000-0002-3527-8622","observed_orcids":["https://orcid.org/0000-0002-3527-8622"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Trevor R. Sorrells","raw_affiliation_strings":["Department of Genetics, Yale University; Howard Hughes Medical Institute; Wu Tsai Institute, Yale University; trevor.sorrells@yale.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, Yale University; Howard Hughes Medical Institute; Wu Tsai Institute, Yale University; trevor.sorrells@yale.edu","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5074556313"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I32971472"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.50597983,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":"234","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12043","display_name":"Insect behavior and control techniques","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aedes-aegypti","display_name":"Aedes aegypti","score":0.9990000128746033},{"id":"https://openalex.org/keywords/mosquito-biting-behavior","display_name":"mosquito biting behavior","score":0.902999997138977},{"id":"https://openalex.org/keywords/optogenetics","display_name":"optogenetics","score":0.9100000262260437},{"id":"https://openalex.org/keywords/neural-circuits","display_name":"neural circuits","score":0.8870000243186951},{"id":"https://openalex.org/keywords/arousal","display_name":"arousal","score":0.3959999978542328},{"id":"https://openalex.org/keywords/olfactory-attraction","display_name":"olfactory attraction","score":0.9169999957084656},{"id":"https://openalex.org/keywords/landing-behavior","display_name":"landing behavior","score":0.7179999947547913},{"id":"https://openalex.org/keywords/engorgement","display_name":"engorgement","score":0.8999999761581421},{"id":"https://openalex.org/keywords/hematophagy","display_name":"blood feeding","score":0.5699999928474426},{"id":"https://openalex.org/keywords/machine-vision","display_name":"machine vision","score":0.7609999775886536},{"id":"https://openalex.org/keywords/behavioral-assays","display_name":"behavioral assays","score":0.5379999876022339},{"id":"https://openalex.org/keywords/vector-borne-disease-vectors","display_name":"vector-borne disease vectors","score":0.593999981880188}],"concepts":[{"id":"https://openalex.org/C50738837","wikidata":"https://www.wikidata.org/wiki/Q781492","display_name":"Optogenetics","level":2,"score":0.8062999844551086},{"id":"https://openalex.org/C2777775583","wikidata":"https://www.wikidata.org/wiki/Q1148004","display_name":"Aedes aegypti","level":3,"score":0.782800018787384},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6872000098228455},{"id":"https://openalex.org/C2780688631","wikidata":"https://www.wikidata.org/wiki/Q310911","display_name":"Aedes","level":3,"score":0.5245000123977661},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3635999858379364},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.29100000858306885},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.2581999897956848},{"id":"https://openalex.org/C2776802408","wikidata":"https://www.wikidata.org/wiki/Q135237","display_name":"Anopheles gambiae","level":3,"score":0.25110000371932983},{"id":"https://openalex.org/C2781375639","wikidata":"https://www.wikidata.org/wiki/Q158597","display_name":"Anopheles","level":3,"score":0.2492000013589859},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.2451999932527542}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.3791/71394","doi":"https://doi.org/10.3791/71394","is_oa":false,"landing_page_url":"https://doi.org/10.3791/71394","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.64898/2026.03.15.711871","doi":"https://doi.org/10.64898/2026.03.15.711871","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.15.711871","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/18/2026.03.15.711871.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:13015328","doi":null,"is_oa":true,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41890001/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13015328/pdf/nihpp-2026.03.15.711871v1.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:13678696","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13678696/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13678696/pdf/nihms-2212814.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:41890001","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41890001","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmid:42631628","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42631628","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.64898/2026.03.15.711871","doi":"https://doi.org/10.64898/2026.03.15.711871","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.15.711871","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/18/2026.03.15.711871.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000914140","display_name":"Identification of mosquito brain neurons that drive blood-feeding behavior","funder_award_id":"DP2AI177891","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320308380","display_name":"Yale University","ror":"https://ror.org/03v76x132"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7203887353.pdf","grobid_xml":"https://content.openalex.org/works/W7203887353.grobid-xml"},"referenced_works_count":43,"referenced_works":["https://openalex.org/W25255938","https://openalex.org/W1532702661","https://openalex.org/W1595045726","https://openalex.org/W1632007921","https://openalex.org/W1967617980","https://openalex.org/W1977591411","https://openalex.org/W1995500583","https://openalex.org/W2023359244","https://openalex.org/W2049121574","https://openalex.org/W2070083683","https://openalex.org/W2093534366","https://openalex.org/W2116999868","https://openalex.org/W2119272664","https://openalex.org/W2146907204","https://openalex.org/W2157071511","https://openalex.org/W2210474179","https://openalex.org/W2301475952","https://openalex.org/W2339932596","https://openalex.org/W2602043016","https://openalex.org/W2754841759","https://openalex.org/W2887114371","https://openalex.org/W2953792681","https://openalex.org/W3004459549","https://openalex.org/W3087157613","https://openalex.org/W3096602324","https://openalex.org/W3165641579","https://openalex.org/W3197998503","https://openalex.org/W3207637518","https://openalex.org/W4210574564","https://openalex.org/W4210593237","https://openalex.org/W4223962547","https://openalex.org/W4226119548","https://openalex.org/W4246945605","https://openalex.org/W4292240463","https://openalex.org/W4306168103","https://openalex.org/W4317681281","https://openalex.org/W4382601940","https://openalex.org/W4391997507","https://openalex.org/W4398209670","https://openalex.org/W4401688066","https://openalex.org/W4401794442","https://openalex.org/W4415703215","https://openalex.org/W4416285092"],"related_works":["https://openalex.org/W7161565960","https://openalex.org/W3087157613","https://openalex.org/W4310457554","https://openalex.org/W4362458879","https://openalex.org/W3040555414","https://openalex.org/W4323531733","https://openalex.org/W2896309457","https://openalex.org/W4404842974","https://openalex.org/W3127252903","https://openalex.org/W4407243563"],"abstract_inverted_index":{"The":[0,170],"mosquito":[1,25,52,205],"Aedes":[2],"aegypti":[3],"is":[4,113],"an":[5],"important":[6],"vector":[7,18],"of":[8,86,165,181,186,197,202],"viral":[9],"pathogens":[10],"and":[11,37,66,75,91,103,132,155,167,183,208],"serves":[12],"as":[13],"a":[14,24,27,55,145,150],"model":[15],"for":[16,46,58,73,120],"other":[17],"species.":[19],"Pathogens":[20],"are":[21,134,142,157],"transmitted":[22],"when":[23],"bites":[26],"host":[28,94],"animal,":[29],"but":[30],"the":[31,51,60,84,195],"neural":[32,48,64],"circuits":[33,65],"that":[34],"control":[35],"seeking":[36],"biting":[38,206],"behavior":[39,182,207],"remain":[40],"unknown.":[41],"This":[42],"paper":[43],"details":[44],"methods":[45,69,189],"manipulating":[47],"activity":[49],"in":[50,83,104,126,149,204],"using":[53],"optogenetics,":[54],"key":[56],"technique":[57],"determining":[59],"causal":[61],"relationship":[62],"between":[63],"behavior.":[67],"These":[68,96,188],"include":[70],"rearing":[71],"mosquitoes":[72,141],"optogenetics":[74],"three":[76],"assays":[77],"designed":[78],"to":[79,106,128,176,193,212],"measure":[80],"different":[81,100,107],"steps":[82],"sequence":[85],"arousal,":[87],"attraction,":[88],"proboscis":[89],"probing,":[90],"engorgement":[92,156],"on":[93],"blood.":[95],"behaviors":[97],"occur":[98],"at":[99],"spatial":[101],"scales":[102],"response":[105,127],"sensory":[108],"stimuli.":[109],"Each":[110],"behavioral":[111],"assay":[112,137],"outfitted":[114],"with":[115,144,159],"red":[116],"(~625":[117],"nm)":[118],"LEDs":[119],"optogenetic":[121],"activation.":[122],"To":[123,136],"detect":[124],"arousal":[125],"olfactory":[129],"stimuli,":[130],"flight":[131],"walking":[133],"quantified.":[135],"attraction":[138],"or":[139],"landing,":[140],"presented":[143],"heated":[146],"blood":[147],"meal":[148],"large":[151],"arena.":[152],"Proboscis":[153],"probing":[154],"assayed":[158],"higher":[160],"resolution":[161],"video,":[162],"enabling":[163],"measurement":[164],"appendages":[166],"abdominal":[168],"size.":[169],"protocol":[171],"describes":[172],"machine":[173],"vision":[174],"models":[175],"enable":[177],"high-resolution":[178],"temporal":[179],"quantification":[180],"endpoint":[184],"measurements":[185],"feeding.":[187],"can":[190,209],"be":[191,210],"used":[192],"test":[194],"role":[196],"any":[198],"genetically":[199],"accessible":[200],"population":[201],"neurons":[203],"extended":[211],"additional":[213],"behaviors.":[214]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-08-22T00:00:00"}