{"id":"https://openalex.org/W7203837596","doi":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","title":"Microsatellite Genotyping of Beluga Whale Samples v1","display_name":"Microsatellite Genotyping of Beluga Whale Samples v1","publication_year":2026,"publication_date":"2026-05-27","ids":{"openalex":"https://openalex.org/W7203837596","doi":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.dm6gp4er5gzp/v1","doi":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","pdf_url":"https://www.protocols.io/view/microsatellite-genotyping-of-beluga-whale-samples-dm6gp4er5gzp/v1.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/microsatellite-genotyping-of-beluga-whale-samples-dm6gp4er5gzp/v1.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021247607","display_name":"Timothy R. Frasier","orcid":"https://orcid.org/0000-0002-3055-0199","observed_orcids":["https://orcid.org/0000-0002-3055-0199"]},"institutions":[{"id":"https://openalex.org/I4405271748","display_name":"Nova Scotia Museum","ror":"https://ror.org/02ywdrw80","country_code":"CA","type":"archive","lineage":["https://openalex.org/I4405271748"]},{"id":"https://openalex.org/I66890659","display_name":"Saint Mary's University","ror":"https://ror.org/010zh7098","country_code":"CA","type":"education","lineage":["https://openalex.org/I66890659"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Timothy Frasier","raw_affiliation_strings":["Saint Mary's University ; Nova Scotia Museum"],"raw_orcid":"https://orcid.org/0000-0002-3055-0199","affiliations":[{"raw_affiliation_string":"Saint Mary's University ; Nova Scotia Museum","institution_ids":["https://openalex.org/I4405271748","https://openalex.org/I66890659"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5123207535","display_name":"Brenna A. Frasier","orcid":"https://orcid.org/0000-0002-8535-1410","observed_orcids":["https://orcid.org/0000-0002-8535-1410"]},"institutions":[{"id":"https://openalex.org/I4405271748","display_name":"Nova Scotia Museum","ror":"https://ror.org/02ywdrw80","country_code":"CA","type":"archive","lineage":["https://openalex.org/I4405271748"]},{"id":"https://openalex.org/I66890659","display_name":"Saint Mary's University","ror":"https://ror.org/010zh7098","country_code":"CA","type":"education","lineage":["https://openalex.org/I66890659"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Brenna Frasier","raw_affiliation_strings":["Saint Mary's University ; Nova Scotia Museum"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Saint Mary's University ; Nova Scotia Museum","institution_ids":["https://openalex.org/I4405271748","https://openalex.org/I66890659"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10659","display_name":"Marine animal studies overview","score":0.026200000196695328,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beluga-whale","display_name":"beluga whale","score":1.0},{"id":"https://openalex.org/keywords/microsatellite-genotyping","display_name":"microsatellite genotyping","score":0.8610000014305115},{"id":"https://openalex.org/keywords/biopsy-specimens","display_name":"biopsy specimens","score":0.9200000166893005},{"id":"https://openalex.org/keywords/multiplex-pcr","display_name":"multiplex PCR","score":0.7860000133514404},{"id":"https://openalex.org/keywords/genetic-polymorphism","display_name":"genetic polymorphism","score":0.5019999742507935},{"id":"https://openalex.org/keywords/cetacean-population-genetics","display_name":"cetacean population genetics","score":0.8339999914169312}],"concepts":[{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.9014000296592712},{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.8740000128746033},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7605999708175659},{"id":"https://openalex.org/C2910798392","wikidata":"https://www.wikidata.org/wiki/Q132072","display_name":"Beluga Whale","level":3,"score":0.7085999846458435},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.5651000142097473},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5604000091552734},{"id":"https://openalex.org/C2777510941","wikidata":"https://www.wikidata.org/wiki/Q191816","display_name":"Beluga","level":2,"score":0.4401000142097473},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.3910999894142151},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.3806999921798706},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.37040001153945923},{"id":"https://openalex.org/C2776224462","wikidata":"https://www.wikidata.org/wiki/Q1527843","display_name":"Right whale","level":3,"score":0.31709998846054077},{"id":"https://openalex.org/C2777704720","wikidata":"https://www.wikidata.org/wiki/Q1865281","display_name":"Whale","level":2,"score":0.30239999294281006},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.28780001401901245},{"id":"https://openalex.org/C2781209916","wikidata":"https://www.wikidata.org/wiki/Q3215756","display_name":"Typing","level":2,"score":0.2809999883174896},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.26510000228881836}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.dm6gp4er5gzp/v1","doi":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","pdf_url":"https://www.protocols.io/view/microsatellite-genotyping-of-beluga-whale-samples-dm6gp4er5gzp/v1.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.dm6gp4er5gzp/v1","doi":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.dm6gp4er5gzp/v1","pdf_url":"https://www.protocols.io/view/microsatellite-genotyping-of-beluga-whale-samples-dm6gp4er5gzp/v1.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7203837596.pdf","grobid_xml":"https://content.openalex.org/works/W7203837596.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2103298863","https://openalex.org/W2032317041","https://openalex.org/W1992852784","https://openalex.org/W2356491028","https://openalex.org/W4394602295","https://openalex.org/W1971505087","https://openalex.org/W7151482703","https://openalex.org/W2071102611","https://openalex.org/W2914564210","https://openalex.org/W2009501216"],"abstract_inverted_index":{"This":[0],"microsatellite":[1,35],"genotyping":[2,12,56],"preparation":[3],"method":[4],"is":[5,48],"used":[6,49,76],"in":[7,71,174],"our":[8,72],"lab":[9],"for":[10,29,69],"routine":[11],"analysis":[13],"of":[14,25,32,38,46],"beluga":[15],"whale":[16],"(Delphinapterus":[17],"leucas)":[18],"biopsy":[19],"samples.":[20],"It":[21],"involves":[22],"the":[23,30,175],"use":[24],"18":[26,33],"primer":[27,169],"pairs":[28],"amplification":[31],"nuclear":[34],"loci,":[36],"17":[37],"which":[39,47,65],"are":[40,59],"informative":[41],"polymorphic":[42],"loci":[43,75],"and":[44],"one*":[45],"as":[50],"a":[51],"\"peacemaker\"":[52],"locus":[53],"to":[54],"ensure":[55],"accuracy.":[57],"Loci":[58],"amplified":[60],"across":[61],"six":[62],"multiplex":[63],"reactions,":[64],"have":[66],"been":[67],"optimized":[68],"performance":[70],"lab.":[73],"The":[74],"are:":[77],"EV14":[78],"(Valsecchi":[79,84,89],"&":[80,85,90],"Amos,":[81,86,91],"1996)":[82,87,92,97,102,107,112,117,122,127,132,152],"EV37Mn":[83],"EV94":[88],"FCB1":[93],"(Buchanan":[94,99,104,109,114,119,124,129],"et":[95,100,105,110,115,120,125,130,135,140,145,150,155,160,165],"al.,":[96,101,106,111,116,121,126,131,136,141,146,151,156,161,166],"FCB3":[98],"FCB4":[103],"FCB5":[108],"FCB6":[113],"FCB10":[118],"FCB14":[123],"FCB17":[128],"MK6":[133],"(Kr\u00fctzen":[134],"2001)":[137],"RW34":[138],"(Waldick":[139,144],"1999)":[142,147,157,162],"RW48":[143],"SW19":[148],"(Richard":[149],"TexVet5":[153],"(Rooney":[154,159],"TexVet19":[158],"*GATA028":[163],"(Palsb\u00f8ll":[164],"1997)":[167],"Detailed":[168],"sequences":[170],"can":[171],"be":[172],"found":[173],"Materials":[176],"section.":[177]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-08-21T00:00:00"}