{"id":"https://openalex.org/W7203824822","doi":"https://doi.org/10.64898/2026.08.19.745742","title":"DAAM1 formin and Ena/VASP proteins assemble functionally distinct actin filaments for focal adhesions","display_name":"DAAM1 formin and Ena/VASP proteins assemble functionally distinct actin filaments for focal adhesions","publication_year":2026,"publication_date":"2026-08-20","ids":{"openalex":"https://openalex.org/W7203824822","doi":"https://doi.org/10.64898/2026.08.19.745742"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.19.745742","doi":"https://doi.org/10.64898/2026.08.19.745742","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.19.745742","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.08.19.745742","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048128013","display_name":"Xiang Le Chua","orcid":"https://orcid.org/0000-0003-4959-8317","observed_orcids":["https://orcid.org/0000-0003-4959-8317"]},"institutions":[{"id":"https://openalex.org/I133731052","display_name":"University of Helsinki","ror":"https://ror.org/040af2s02","country_code":"FI","type":"education","lineage":["https://openalex.org/I133731052"]},{"id":"https://openalex.org/I4391768107","display_name":"HiLIFE \u2013 El\u00e4m\u00e4ntieteiden Instituutti","ror":"https://ror.org/018bh0m68","country_code":"FI","type":"facility","lineage":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Xiang Le Chua","raw_affiliation_strings":["HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland"],"raw_orcid":"https://orcid.org/0000-0003-4959-8317","affiliations":[{"raw_affiliation_string":"HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]},{"raw_affiliation_string":"Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019278040","display_name":"Parijat Biswas","orcid":"https://orcid.org/0000-0003-2235-2384","observed_orcids":["https://orcid.org/0000-0003-2235-2384"]},"institutions":[{"id":"https://openalex.org/I133731052","display_name":"University of Helsinki","ror":"https://ror.org/040af2s02","country_code":"FI","type":"education","lineage":["https://openalex.org/I133731052"]},{"id":"https://openalex.org/I4391768107","display_name":"HiLIFE \u2013 El\u00e4m\u00e4ntieteiden Instituutti","ror":"https://ror.org/018bh0m68","country_code":"FI","type":"facility","lineage":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Parijat Biswas","raw_affiliation_strings":["HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland"],"raw_orcid":"https://orcid.org/0000-0003-2235-2384","affiliations":[{"raw_affiliation_string":"HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]},{"raw_affiliation_string":"Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041524960","display_name":"Hugo Wioland","orcid":"https://orcid.org/0000-0001-5254-9642","observed_orcids":["https://orcid.org/0000-0001-5254-9642"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I204730241","display_name":"Universit\u00e9 Paris Cit\u00e9","ror":"https://ror.org/05f82e368","country_code":"FR","type":"education","lineage":["https://openalex.org/I204730241"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Hugo Wioland","raw_affiliation_strings":["Universit\u00e9 Paris-Cit\u00e9, CNRS, Institut Jacques Monod, 75013 Paris, France"],"raw_orcid":"https://orcid.org/0000-0001-5254-9642","affiliations":[{"raw_affiliation_string":"Universit\u00e9 Paris-Cit\u00e9, CNRS, Institut Jacques Monod, 75013 Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I204730241","https://openalex.org/I4210113761"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013579145","display_name":"Pekka Lappalainen","orcid":"https://orcid.org/0000-0001-6227-0354","observed_orcids":["https://orcid.org/0000-0001-6227-0354"]},"institutions":[{"id":"https://openalex.org/I133731052","display_name":"University of Helsinki","ror":"https://ror.org/040af2s02","country_code":"FI","type":"education","lineage":["https://openalex.org/I133731052"]},{"id":"https://openalex.org/I4391768107","display_name":"HiLIFE \u2013 El\u00e4m\u00e4ntieteiden Instituutti","ror":"https://ror.org/018bh0m68","country_code":"FI","type":"facility","lineage":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Pekka Lappalainen","raw_affiliation_strings":["HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland"],"raw_orcid":"https://orcid.org/0000-0001-6227-0354","affiliations":[{"raw_affiliation_string":"HiLIFE Institute of Biotechnology, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052","https://openalex.org/I4391768107"]},{"raw_affiliation_string":"Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki 00014, Finland","institution_ids":["https://openalex.org/I133731052"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5013579145"],"corresponding_institution_ids":["https://openalex.org/I133731052","https://openalex.org/I4391768107"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ena-vasp-proteins","display_name":"Ena/VASP proteins","score":0.9509999752044678},{"id":"https://openalex.org/keywords/actin-filament-assembly","display_name":"actin filament assembly","score":0.8679999709129333},{"id":"https://openalex.org/keywords/focal-adhesions","display_name":"focal adhesions","score":0.9779999852180481},{"id":"https://openalex.org/keywords/tropomyosin-isoforms","display_name":"tropomyosin isoforms","score":0.828000009059906},{"id":"https://openalex.org/keywords/alpha-actinin","display_name":"alpha-actinin","score":0.8629999756813049},{"id":"https://openalex.org/keywords/actin-filament-bundles","display_name":"actin filament bundles","score":0.5640000104904175},{"id":"https://openalex.org/keywords/focal-adhesion-disassembly","display_name":"focal adhesion disassembly","score":0.8009999990463257},{"id":"https://openalex.org/keywords/focal-adhesion-maturation","display_name":"focal adhesion maturation","score":0.9589999914169312},{"id":"https://openalex.org/keywords/actin-cytoskeleton","display_name":"actin cytoskeleton","score":0.7300000190734863}],"concepts":[{"id":"https://openalex.org/C53195969","wikidata":"https://www.wikidata.org/wiki/Q5470213","display_name":"Formins","level":5,"score":0.9009000062942505},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7131999731063843},{"id":"https://openalex.org/C151166631","wikidata":"https://www.wikidata.org/wiki/Q904514","display_name":"Focal adhesion","level":3,"score":0.6517000198364258},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.6266000270843506},{"id":"https://openalex.org/C14228908","wikidata":"https://www.wikidata.org/wiki/Q2920483","display_name":"Protein filament","level":2,"score":0.5914000272750854},{"id":"https://openalex.org/C59999672","wikidata":"https://www.wikidata.org/wiki/Q21497084","display_name":"MDia1","level":5,"score":0.5200999975204468},{"id":"https://openalex.org/C130861313","wikidata":"https://www.wikidata.org/wiki/Q3775363","display_name":"Actin-binding protein","level":5,"score":0.5113999843597412},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4544999897480011},{"id":"https://openalex.org/C3620293","wikidata":"https://www.wikidata.org/wiki/Q14908027","display_name":"Actin remodeling","level":5,"score":0.43309998512268066},{"id":"https://openalex.org/C205875245","wikidata":"https://www.wikidata.org/wiki/Q418454","display_name":"Tropomyosin","level":3,"score":0.4284999966621399},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.4092999994754791},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3643999993801117},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.3402000069618225},{"id":"https://openalex.org/C151000934","wikidata":"https://www.wikidata.org/wiki/Q4676834","display_name":"Actin remodeling of neurons","level":5,"score":0.32589998841285706},{"id":"https://openalex.org/C109185818","wikidata":"https://www.wikidata.org/wiki/Q3092607","display_name":"Lamellipodium","level":4,"score":0.3089999854564667},{"id":"https://openalex.org/C78383274","wikidata":"https://www.wikidata.org/wiki/Q421987","display_name":"Intermediate filament","level":4,"score":0.29319998621940613},{"id":"https://openalex.org/C133702464","wikidata":"https://www.wikidata.org/wiki/Q3487083","display_name":"Actinin","level":4,"score":0.2754000127315521},{"id":"https://openalex.org/C108391706","wikidata":"https://www.wikidata.org/wiki/Q15333532","display_name":"PTK2","level":5,"score":0.2702000141143799}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.64898/2026.08.19.745742","doi":"https://doi.org/10.64898/2026.08.19.745742","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.19.745742","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:HAL:hal-05770766v1","doi":null,"is_oa":true,"landing_page_url":"https://hal.science/hal-05770766","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"2026","raw_type":"info:eu-repo/semantics/preprint"}],"best_oa_location":{"id":"doi:10.64898/2026.08.19.745742","doi":"https://doi.org/10.64898/2026.08.19.745742","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.19.745742","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":63,"referenced_works":["https://openalex.org/W1600241919","https://openalex.org/W1967487834","https://openalex.org/W1967596069","https://openalex.org/W1971766102","https://openalex.org/W1977709885","https://openalex.org/W1990850141","https://openalex.org/W1992381289","https://openalex.org/W1994490125","https://openalex.org/W1999663047","https://openalex.org/W2007391251","https://openalex.org/W2007821852","https://openalex.org/W2010722403","https://openalex.org/W2018987816","https://openalex.org/W2029042183","https://openalex.org/W2030768863","https://openalex.org/W2050612706","https://openalex.org/W2055188333","https://openalex.org/W2055257312","https://openalex.org/W2064071063","https://openalex.org/W2066406174","https://openalex.org/W2070119451","https://openalex.org/W2075966687","https://openalex.org/W2078684403","https://openalex.org/W2082848316","https://openalex.org/W2085767196","https://openalex.org/W2108162507","https://openalex.org/W2122782850","https://openalex.org/W2135459242","https://openalex.org/W2145123926","https://openalex.org/W2153290005","https://openalex.org/W2163455170","https://openalex.org/W2211191608","https://openalex.org/W2521453400","https://openalex.org/W2535749862","https://openalex.org/W2588726331","https://openalex.org/W2613380886","https://openalex.org/W2626922536","https://openalex.org/W2727776884","https://openalex.org/W2801720723","https://openalex.org/W2811796540","https://openalex.org/W2897402129","https://openalex.org/W2945635822","https://openalex.org/W2980369384","https://openalex.org/W2980580264","https://openalex.org/W2981867496","https://openalex.org/W3004594796","https://openalex.org/W3022104547","https://openalex.org/W3089477642","https://openalex.org/W3092324095","https://openalex.org/W3125041038","https://openalex.org/W3205850006","https://openalex.org/W3215355005","https://openalex.org/W4220996774","https://openalex.org/W4241859772","https://openalex.org/W4289520791","https://openalex.org/W4378194817","https://openalex.org/W4392876302","https://openalex.org/W4393032128","https://openalex.org/W4394728154","https://openalex.org/W4399381836","https://openalex.org/W4400318396","https://openalex.org/W4413112599","https://openalex.org/W4416640843"],"related_works":["https://openalex.org/W4320890994","https://openalex.org/W4393032128","https://openalex.org/W4414958327","https://openalex.org/W1982270174","https://openalex.org/W2148156439","https://openalex.org/W4220996774","https://openalex.org/W6958059149","https://openalex.org/W2075966687","https://openalex.org/W2666264305","https://openalex.org/W1996900289"],"abstract_inverted_index":{"SUMMARY":[0],"Eukaryotic":[1],"cells":[2],"contain":[3],"multiple":[4],"biochemically":[5,141],"distinct":[6,44,144],"actin":[7,15,28,45,69,86,146],"filament":[8,29,87,147],"networks,":[9],"which":[10,26],"enable":[11],"versatile":[12],"functions":[13],"of":[14,19,68,94,121],"in":[16,33,139,149],"a":[17,34],"range":[18],"cellular":[20],"processes.":[21],"Yet,":[22],"the":[23],"mechanisms":[24],"by":[25,49],"specific":[27,131],"networks":[30],"are":[31,57],"assembled":[32,58],"common":[35],"cytoplasm":[36],"remain":[37],"elusive.":[38],"Here,":[39],"we":[40,71],"investigated":[41],"how":[42],"functionally":[43,143],"nanoscale":[46],"layers,":[47],"specified":[48],"\u03b1-actinin":[50,84,122],"and":[51,55,100,119,135,142],"tropomyosin":[52],"isoforms,":[53],"Tpm1.6":[54],"Tpm3.2,":[56],"at":[59,89],"focal":[60,90,102,116,124],"adhesions.":[61,91,125],"By":[62],"combining":[63],"genetic":[64],"perturbations":[65],"with":[66],"mitochondrial-targeting":[67],"polymerases,":[70],"discovered":[72],"that":[73],"DAAM1":[74,95],"formin":[75,134],"assembles":[76],"Tpm3.2-actin":[77],"filaments,":[78],"whereas":[79],"Ena/VASP":[80,111,136],"family":[81,137],"proteins":[82,138],"polymerize":[83],"cross-linked":[85],"bundles":[88],"Consequently,":[92],"loss":[93,120],"dampened":[96],"Tpm3.2":[97],"protein":[98],"levels":[99],"impaired":[101],"adhesion":[103,117],"disassembly,":[104],"thus":[105],"phenocopying":[106],"Tpm3.2-deficient":[107],"cells.":[108,150],"In":[109],"contrast,":[110],"depletion":[112],"led":[113],"to":[114],"defective":[115],"maturation":[118],"from":[123],"More":[126],"broadly,":[127],"our":[128],"study":[129],"highlights":[130],"roles":[132],"for":[133],"assembling":[140],"linear":[145],"arrays":[148]},"counts_by_year":[{"year":2026,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-08-21T00:00:00"}