{"id":"https://openalex.org/W7203598765","doi":"https://doi.org/10.1093/ajrcmb/aanag164","title":"Modified RNA Extraction Methods to Eliminate Agarose Impurities in Human Precision-Cut Lung Slices","display_name":"Modified RNA Extraction Methods to Eliminate Agarose Impurities in Human Precision-Cut Lung Slices","publication_year":2026,"publication_date":"2026-08-14","ids":{"openalex":"https://openalex.org/W7203598765","doi":"https://doi.org/10.1093/ajrcmb/aanag164","pmid":"https://pubmed.ncbi.nlm.nih.gov/42606537"},"language":"en","primary_location":{"id":"doi:10.1093/ajrcmb/aanag164","doi":"https://doi.org/10.1093/ajrcmb/aanag164","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ajrcmb/aanag164","pdf_url":null,"source":{"id":"https://openalex.org/S171155024","display_name":"American Journal of Respiratory Cell and Molecular Biology","issn_l":"1044-1549","issn":["1044-1549","1535-4989"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310316311","host_organization_name":"American Thoracic Society","host_organization_lineage":["https://openalex.org/P4310316311"],"host_organization_lineage_names":["American Thoracic Society"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Respiratory Cell and Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1093/ajrcmb/aanag164","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5123619219","display_name":"Reina Rangel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Reina Rangel","raw_affiliation_strings":["Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5125630545","display_name":"Sabina Anderson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sabina Anderson","raw_affiliation_strings":["Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035633851","display_name":"Giuseppe DeIuliis","orcid":"https://orcid.org/0000-0003-0841-8156","observed_orcids":["https://orcid.org/0000-0003-0841-8156"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Giuseppe DeIuliis","raw_affiliation_strings":["Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":"https://orcid.org/0000-0003-0841-8156","affiliations":[{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089823979","display_name":"Edward P. Manning","orcid":"https://orcid.org/0000-0002-4430-8168","observed_orcids":["https://orcid.org/0000-0002-4430-8168"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]},{"id":"https://openalex.org/I4210086971","display_name":"VA Connecticut Healthcare System","ror":"https://ror.org/000rgm762","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210086971","https://openalex.org/I4210095851"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward P Manning","raw_affiliation_strings":["VA Connecticut Healthcare System , West Haven, CT,","Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":"https://orcid.org/0000-0002-4430-8168","affiliations":[{"raw_affiliation_string":"VA Connecticut Healthcare System , West Haven, CT,","institution_ids":["https://openalex.org/I4210086971"]},{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082834998","display_name":"Farida Ahangari","orcid":"https://orcid.org/0000-0002-4144-9395","observed_orcids":["https://orcid.org/0000-0002-4144-9395"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Farida Ahangari","raw_affiliation_strings":["Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":"https://orcid.org/0000-0002-4144-9395","affiliations":[{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027018298","display_name":"Abhay S. Pandit","orcid":"https://orcid.org/0000-0002-6292-4933","observed_orcids":["https://orcid.org/0000-0002-6292-4933"]},"institutions":[{"id":"https://openalex.org/I188760350","display_name":"Ollscoil na Gaillimhe \u2013 University of Galway","ror":"https://ror.org/03bea9k73","country_code":"IE","type":"education","lineage":["https://openalex.org/I188760350"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"Abhay Pandit","raw_affiliation_strings":["C\u00daRAM Research Ireland Centre for Medical Devices, University of Galway ,"],"raw_orcid":"https://orcid.org/0000-0002-6292-4933","affiliations":[{"raw_affiliation_string":"C\u00daRAM Research Ireland Centre for Medical Devices, University of Galway ,","institution_ids":["https://openalex.org/I188760350"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088823841","display_name":"Naftali Kaminski","orcid":"https://orcid.org/0000-0001-5917-4601","observed_orcids":["https://orcid.org/0000-0001-5917-4601"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Naftali Kaminski","raw_affiliation_strings":["Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":"https://orcid.org/0000-0001-5917-4601","affiliations":[{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030398148","display_name":"Joan Mart\u00ed-Mu\u00f1oz","orcid":"https://orcid.org/0000-0002-6102-9590","observed_orcids":["https://orcid.org/0000-0002-6102-9590"]},"institutions":[{"id":"https://openalex.org/I188760350","display_name":"Ollscoil na Gaillimhe \u2013 University of Galway","ror":"https://ror.org/03bea9k73","country_code":"IE","type":"education","lineage":["https://openalex.org/I188760350"]},{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["IE","US"],"is_corresponding":false,"raw_author_name":"Joan Marti-Munoz","raw_affiliation_strings":["C\u00daRAM Research Ireland Centre for Medical Devices, University of Galway ,","Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,"],"raw_orcid":"https://orcid.org/0000-0002-6102-9590","affiliations":[{"raw_affiliation_string":"C\u00daRAM Research Ireland Centre for Medical Devices, University of Galway ,","institution_ids":["https://openalex.org/I188760350"]},{"raw_affiliation_string":"Yale School of Medicine Section of Pulmonary Critical Care & Sleep Medicine, , New Haven, CT,","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.53182772,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/precision-cut-lung-slices","display_name":"precision-cut lung slices","score":0.996999979019165},{"id":"https://openalex.org/keywords/rna-extraction","display_name":"RNA extraction","score":0.9179999828338623},{"id":"https://openalex.org/keywords/rna-integrity","display_name":"RNA integrity","score":0.7710000276565552},{"id":"https://openalex.org/keywords/phenol-contamination","display_name":"phenol contamination","score":0.8679999709129333},{"id":"https://openalex.org/keywords/housekeeping-gene-expression","display_name":"housekeeping gene expression","score":0.5580000281333923},{"id":"https://openalex.org/keywords/human-lung-tissue","display_name":"human lung tissue","score":0.6700000166893005}],"concepts":[{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.9362000226974487},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.8101999759674072},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.7251999974250793},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5781000256538391},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5647000074386597},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.5285999774932861},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4659999907016754},{"id":"https://openalex.org/C71987851","wikidata":"https://www.wikidata.org/wiki/Q7216430","display_name":"Impurity","level":2,"score":0.4481000006198883},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34119999408721924},{"id":"https://openalex.org/C33922130","wikidata":"https://www.wikidata.org/wiki/Q419232","display_name":"Housekeeping gene","level":4,"score":0.31850001215934753},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.31450000405311584},{"id":"https://openalex.org/C88380143","wikidata":"https://www.wikidata.org/wiki/Q416674","display_name":"Dissolution","level":2,"score":0.31290000677108765},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.302700012922287},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2985000014305115},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.275299996137619},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.26409998536109924}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1093/ajrcmb/aanag164","doi":"https://doi.org/10.1093/ajrcmb/aanag164","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ajrcmb/aanag164","pdf_url":null,"source":{"id":"https://openalex.org/S171155024","display_name":"American Journal of Respiratory Cell and Molecular Biology","issn_l":"1044-1549","issn":["1044-1549","1535-4989"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310316311","host_organization_name":"American Thoracic Society","host_organization_lineage":["https://openalex.org/P4310316311"],"host_organization_lineage_names":["American Thoracic Society"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Respiratory Cell and Molecular Biology","raw_type":"journal-article"},{"id":"pmid:42606537","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42606537","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of respiratory cell and molecular biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1093/ajrcmb/aanag164","doi":"https://doi.org/10.1093/ajrcmb/aanag164","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ajrcmb/aanag164","pdf_url":null,"source":{"id":"https://openalex.org/S171155024","display_name":"American Journal of Respiratory Cell and Molecular Biology","issn_l":"1044-1549","issn":["1044-1549","1535-4989"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310316311","host_organization_name":"American Thoracic Society","host_organization_lineage":["https://openalex.org/P4310316311"],"host_organization_lineage_names":["American Thoracic Society"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Respiratory Cell and Molecular Biology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4043000042438507}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7129574810","https://openalex.org/W7161545337","https://openalex.org/W2592220459","https://openalex.org/W4404882795","https://openalex.org/W3096976218","https://openalex.org/W3175604852","https://openalex.org/W3148326602","https://openalex.org/W1993005339","https://openalex.org/W7143370078","https://openalex.org/W6939290855"],"abstract_inverted_index":{"Precision-cut":[0],"lung":[1,17],"slices":[2],"(PCLS)":[3],"have":[4],"emerged":[5],"as":[6,220,222,231],"a":[7,54,84,178],"powerful":[8],"tool":[9],"for":[10,57,236],"studying":[11],"the":[12,20,103,111,125,144,163,169,232],"biology":[13],"of":[14,22,47,87,95,149,162,183],"viable":[15],"human":[16],"tissue.":[18],"However,":[19],"presence":[21,148],"agarose":[23,43,48,96,99,175,215],"impurities":[24,44,100,151,216],"compromises":[25],"RNA":[26,59,66,75,88,104,164,218,227],"yield":[27],"and":[28,69,77,93,101,118,127,138,154,166,174,189,234,238],"integrity":[29,78,128,239],"during":[30,217],"extraction.":[31,89,201],"We":[32,202],"tested":[33],"whether":[34],"using":[35,83,168],"an":[36,160],"alternative":[37,210],"Plant":[38,91,155,172],"kit":[39,92,156,173],"extraction":[40],"to":[41,114,134,159,208,223],"wash":[42],"or":[45],"pre-dissolution":[46],"from":[49,106,130],"PCLS":[50,206],"by":[51,124,143,194],"immersion":[52],"in":[53,61,152],"dissolving":[55],"buffer":[56],"routine":[58],"isolation":[60],"gel":[62],"electrophoresis":[63],"would":[64],"improve":[65],"quantity,":[67],"quality,":[68],"integrity.":[70],"Our":[71],"results":[72],"show":[73],"that":[74,212],"quantity":[76,105,165],"are":[79],"highly":[80],"compromised":[81],"when":[82],"conventional":[85,153,200],"method":[86],"The":[90,147,171],"dissolution":[94,176],"efficiently":[97,213],"removed":[98],"increased":[102],"<":[107,187,192],"0.028":[108],"\u00b5g/PCLS":[109,122],"(below":[110],"detection":[112],"limit)":[113],"0.42":[115],"\u00b1":[116,120,132,136,140],"0.11":[117],"0.65":[119],"0.17":[121],"(measured":[123,142],"Qubit)":[126],"number":[129],"2.27":[131],"0.87":[133],"6.60":[135],"0.59":[137],"9.13":[139],"0.39":[141],"Bioanalyzer),":[145],"respectively.":[146],"phenol":[150],"extractions":[157],"led":[158],"overestimation":[161],"quality":[167],"NanoDrop.":[170],"showed":[177],"significant":[179],"transcription":[180],"amplification":[181],"increase":[182],"housekeeping":[184],"GUSB":[185],"(p":[186,191],"0.0001)":[188],"COL1A1":[190],"0.05)":[193],"qPCR,":[195],"validating":[196],"these":[197],"methods":[198,211],"over":[199],"encourage":[203],"laboratories":[204],"applying":[205],"experimentation":[207],"implement":[209],"remove":[214],"extraction,":[219],"well":[221],"rely":[224],"on":[225],"sensitive":[226],"quantitative":[228],"techniques,":[229],"such":[230],"Qubit":[233],"Bioanalyzer,":[235],"quantification":[237],"assessment.":[240]},"counts_by_year":[],"updated_date":"2026-09-24T07:57:37.273135","created_date":"2026-08-18T00:00:00"}