{"id":"https://openalex.org/W7203572447","doi":"https://doi.org/10.6084/m9.figshare.33265310","title":"Sequalae and reversal of age-dependent alterations in mitochondrial dynamics via autophagy enhancement in reprogrammed human neurons","display_name":"Sequalae and reversal of age-dependent alterations in mitochondrial dynamics via autophagy enhancement in reprogrammed human neurons","publication_year":2026,"publication_date":"2026-08-16","ids":{"openalex":"https://openalex.org/W7203572447","doi":"https://doi.org/10.6084/m9.figshare.33265310"},"language":"en","primary_location":{"id":"doi:10.6084/m9.figshare.33265310","doi":"https://doi.org/10.6084/m9.figshare.33265310","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.33265310","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},"volume":null,"type":"dataset","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6084/m9.figshare.33265310","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088037049","display_name":"Eva Klinman","orcid":"https://orcid.org/0000-0003-1427-0194","observed_orcids":["https://orcid.org/0000-0003-1427-0194"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eva Klinman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5147263021","display_name":"Ji-Sun Kwon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ji-Sun Kwon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049878377","display_name":"Roland E. Dolle","orcid":"https://orcid.org/0000-0001-5574-6019","observed_orcids":["https://orcid.org/0000-0001-5574-6019"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roland E. Dolle","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049586920","display_name":"Stephen C. Pak","orcid":"https://orcid.org/0000-0002-8089-3237","observed_orcids":["https://orcid.org/0000-0002-8089-3237"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Stephen C. Pak","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043959484","display_name":"Gary A. Silverman","orcid":"https://orcid.org/0000-0002-2686-2843","observed_orcids":["https://orcid.org/0000-0002-2686-2843"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gary A. Silverman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044222068","display_name":"David H. Perlmutter","orcid":"https://orcid.org/0000-0001-6112-7652","observed_orcids":["https://orcid.org/0000-0001-6112-7652"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David H. Perlmutter","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5091747215","display_name":"Andrew S.J. Yoo","orcid":"https://orcid.org/0000-0002-0304-3247","observed_orcids":["https://orcid.org/0000-0002-0304-3247"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andrew S. Yoo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.004999999888241291,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10676","display_name":"Genetics, Aging, and Longevity in Model Organisms","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1302","display_name":"Aging"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neuronal-aging","display_name":"neuronal aging","score":0.7590000033378601},{"id":"https://openalex.org/keywords/mitochondrial-dynamics","display_name":"mitochondrial dynamics","score":0.9150000214576721},{"id":"https://openalex.org/keywords/mitochondrial-fission-and-fusion","display_name":"mitochondrial fission and fusion","score":0.8920000195503235},{"id":"https://openalex.org/keywords/autophagy","display_name":"autophagy","score":0.9769999980926514},{"id":"https://openalex.org/keywords/macroautophagy","display_name":"macroautophagy","score":0.6869999766349792},{"id":"https://openalex.org/keywords/tfeb","display_name":"TFEB","score":0.8220000267028809},{"id":"https://openalex.org/keywords/mitophagy","display_name":"mitophagy","score":0.9869999885559082},{"id":"https://openalex.org/keywords/mitochondrial-membrane-potential","display_name":"mitochondrial membrane potential","score":0.9639999866485596},{"id":"https://openalex.org/keywords/human-neurons","display_name":"human neurons","score":0.3490000069141388}],"concepts":[{"id":"https://openalex.org/C2779324830","wikidata":"https://www.wikidata.org/wiki/Q1806192","display_name":"Mitophagy","level":4,"score":0.8575999736785889},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.8070999979972839},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7875000238418579},{"id":"https://openalex.org/C17619807","wikidata":"https://www.wikidata.org/wiki/Q6881852","display_name":"Mitochondrial fission","level":3,"score":0.7678999900817871},{"id":"https://openalex.org/C2777755479","wikidata":"https://www.wikidata.org/wiki/Q18039927","display_name":"FIS1","level":5,"score":0.7402999997138977},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.7384999990463257},{"id":"https://openalex.org/C163950672","wikidata":"https://www.wikidata.org/wiki/Q14905375","display_name":"mitochondrial fusion","level":4,"score":0.7084000110626221},{"id":"https://openalex.org/C2777324860","wikidata":"https://www.wikidata.org/wiki/Q18034992","display_name":"DNM1L","level":4,"score":0.6991999745368958},{"id":"https://openalex.org/C168296220","wikidata":"https://www.wikidata.org/wiki/Q14882441","display_name":"Autophagosome","level":4,"score":0.6812000274658203},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.6395999789237976},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.532800018787384},{"id":"https://openalex.org/C2777092462","wikidata":"https://www.wikidata.org/wiki/Q15334407","display_name":"MFN2","level":5,"score":0.5144000053405762},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.47540000081062317},{"id":"https://openalex.org/C2780216420","wikidata":"https://www.wikidata.org/wiki/Q83330","display_name":"Lysosome","level":3,"score":0.38109999895095825},{"id":"https://openalex.org/C500499127","wikidata":"https://www.wikidata.org/wiki/Q332154","display_name":"Ageing","level":2,"score":0.3725999891757965},{"id":"https://openalex.org/C103038307","wikidata":"https://www.wikidata.org/wiki/Q6556360","display_name":"Lipid bilayer fusion","level":3,"score":0.3424000144004822},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3353999853134155},{"id":"https://openalex.org/C61081601","wikidata":"https://www.wikidata.org/wiki/Q1102066","display_name":"Microtubule-associated protein","level":3,"score":0.335099995136261},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.3133000135421753},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.30720001459121704},{"id":"https://openalex.org/C13591479","wikidata":"https://www.wikidata.org/wiki/Q6881880","display_name":"Mitochondrial permeability transition pore","level":4,"score":0.30630001425743103},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.30489999055862427},{"id":"https://openalex.org/C522857546","wikidata":"https://www.wikidata.org/wiki/Q2070979","display_name":"Senescence","level":2,"score":0.30410000681877136},{"id":"https://openalex.org/C111491248","wikidata":"https://www.wikidata.org/wiki/Q18033821","display_name":"DNAJA3","level":5,"score":0.30239999294281006},{"id":"https://openalex.org/C155432671","wikidata":"https://www.wikidata.org/wiki/Q17073362","display_name":"Organelle biogenesis","level":4,"score":0.28700000047683716},{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.28209999203681946},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.2651999890804291},{"id":"https://openalex.org/C181911157","wikidata":"https://www.wikidata.org/wiki/Q389844","display_name":"Membrane potential","level":2,"score":0.26080000400543213},{"id":"https://openalex.org/C2780042272","wikidata":"https://www.wikidata.org/wiki/Q27548992","display_name":"ATG8","level":4,"score":0.2549999952316284},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.2500999867916107},{"id":"https://openalex.org/C2776804853","wikidata":"https://www.wikidata.org/wiki/Q18045837","display_name":"PINK1","level":5,"score":0.2500999867916107}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.6084/m9.figshare.33265310","doi":"https://doi.org/10.6084/m9.figshare.33265310","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.33265310","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},{"id":"doi:10.6084/m9.figshare.33265310.v1","doi":"https://doi.org/10.6084/m9.figshare.33265310.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.33265310.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},{"id":"doi:10.6084/m9.figshare.33265310.v2","doi":"https://doi.org/10.6084/m9.figshare.33265310.v2","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.33265310.v2","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.6084/m9.figshare.33265310","doi":"https://doi.org/10.6084/m9.figshare.33265310","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.33265310","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4414775368","https://openalex.org/W7223050126","https://openalex.org/W4295926764","https://openalex.org/W4293554994","https://openalex.org/W6893024543","https://openalex.org/W4403129766","https://openalex.org/W2036229829","https://openalex.org/W4406276627","https://openalex.org/W3016857603","https://openalex.org/W4389019123"],"abstract_inverted_index":{"How":[0],"aging":[1,226],"of":[2,7,30,36,65,86,93,125,234,244,296],"human":[3,40],"neurons":[4,21,50,151],"affects":[5],"dynamics":[6],"essential":[8],"organelle":[9,208],"such":[10],"as":[11],"mitochondria":[12,126],"and":[13,68,114,123,162,201,203],"autophagosomes":[14],"remains":[15],"largely":[16],"unknown.":[17],"MicroRNA-induced":[18],"directly":[19],"reprogrammed":[20],"(miNs)":[22],"derived":[23,51,152],"from":[24,52,91,153],"adult":[25],"fibroblasts":[26],"retain":[27],"age-associated":[28],"signatures":[29],"the":[31,34,63,79,120,195],"donor,":[32],"enabling":[33],"study":[35],"age-dependent":[37,129,165,179,196],"features":[38],"in":[39,89,111,139,147,150,167,171,181,198,238],"neurons,":[41],"including":[42],"longitudinal":[43],"isogenic":[44],"samples.":[45],"Transcriptomic":[46],"analysis":[47],"revealed":[48],"that":[49,119],"elderly":[53],"individuals":[54,154],"are":[55],"characterized":[56],"by":[57,186,224],"gene":[58],"expression":[59],"changes":[60,77,138],"associated":[61,264,270,278],"with":[62,107],"regulation":[64],"autophagosomes,":[66],"lysosomes,":[67,236],"mitochondria,":[69],"compared":[70,158],"to":[71,137,159,175,231,241],"young":[72],"counterparts.":[73],"To":[74],"clarify":[75],"these":[76],"at":[78],"cellular":[80,87],"level,":[81],"we":[82],"performed":[83],"live-cell":[84],"imaging":[85],"organelles":[88],"miNs":[90,98],"donors":[92],"different":[94],"ages.":[95],"Older":[96],"donor":[97],"exhibit":[99],"decreased":[100],"mitochondrial":[101,112,133,199,205,216,288],"membrane":[102,265,289],"potential,":[103],"which":[104,192,225],"surprisingly":[105],"co-occurs":[106],"a":[108,144,163,222,232],"significant":[109,145],"increase":[110,197],"fission":[113,122,200],"fusion":[115,124,202],"events.":[116],"We":[117,141],"posit":[118],"increased":[121],"may":[127],"reflect":[128],"compensation":[130],"for":[131],"impaired":[132],"turnover,":[134],"perhaps":[135],"due":[136],"macroautophagy/autophagy.":[140],"subsequently":[142],"identified":[143],"decrease":[146],"autophagosome":[148,188],"acidification":[149],">":[155],"65":[156],"years":[157],"younger":[160],"donors,":[161],"corresponding":[164],"reduction":[166],"neuritic":[168,235],"lysosomes":[169,173],"resulting":[170,237],"fewer":[172],"available":[174],"acidify":[176],"autophagosomes.":[177],"This":[178],"deficit":[180],"autolysosome":[182],"flux":[183,229],"was":[184],"rescued":[185],"promoting":[187],"generation":[189],"through":[190],"TFEB,":[191],"also":[193],"reversed":[194],"improved":[204],"health.":[206],"Partial":[207],"recovery":[209],"occurred":[210],"after":[211],"inducing":[212],"mitophagy":[213],"or":[214],"inhibiting":[215],"fission.":[217],"Together,":[218],"this":[219],"work":[220],"reveals":[221],"mechanism":[223],"reduces":[227],"autophagic":[228],"secondary":[230],"loss":[233,243],"mitochondria-intrinsic":[239],"mechanisms":[240],"avoid":[242],"energy":[245],"production.":[246],"Abbreviations:":[247],"DEG:":[248],"differentially":[249],"expressed":[250],"gene;":[251],"DMSO:":[252],"dimethyl":[253],"sulfoxide;":[254],"Dox:":[255],"doxycycline;":[256],"iPSC:":[257],"induced":[258,283],"pluripotent":[259],"stem":[260],"cell;":[261],"LAMP1:":[262],"lysosome":[263],"protein":[266,271,279],"1;":[267,298],"MAP1LC3/LC3:":[268],"microtubule":[269,277],"1":[272],"light":[273],"chain":[274],"3;":[275],"MAPT/tau:":[276],"tau;":[280],"miNs:":[281],"microRNA":[282],"neurons;":[284],"miRNA:":[285],"microRNA;":[286],"MMP:":[287],"potential;":[290],"PID:":[291],"post-infection":[292],"day;":[293],"RCAN1:":[294],"regulator":[295],"calcineurin":[297],"TFEB:":[299],"transcription":[300],"factor":[301],"EB;":[302],"TUBB3:":[303],"tubulin":[304],"beta":[305],"3":[306],"class":[307],"III;":[308],"UA:":[309],"urolithin":[310],"A.":[311]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-08-17T00:00:00"}