{"id":"https://openalex.org/W7202397252","doi":"https://doi.org/10.9734/jabb/2026/v29i94295","title":"Molecular Detection of Anaplasma marginale Infection in Carrier Cattle by MSP5 Gene-Based Semi-Nested PCR","display_name":"Molecular Detection of Anaplasma marginale Infection in Carrier Cattle by MSP5 Gene-Based Semi-Nested PCR","publication_year":2026,"publication_date":"2026-08-13","ids":{"openalex":"https://openalex.org/W7202397252","doi":"https://doi.org/10.9734/jabb/2026/v29i94295"},"language":"en","primary_location":{"id":"doi:10.9734/jabb/2026/v29i94295","doi":"https://doi.org/10.9734/jabb/2026/v29i94295","is_oa":true,"landing_page_url":"https://doi.org/10.9734/jabb/2026/v29i94295","pdf_url":null,"source":{"id":"https://openalex.org/S2764613355","display_name":"Journal of Advances in Biology & Biotechnology","issn_l":"2394-1081","issn":["2394-1081"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320855","host_organization_name":"Sciencedomain International","host_organization_lineage":["https://openalex.org/P4310320855"],"host_organization_lineage_names":["Sciencedomain International"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Biology & Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.9734/jabb/2026/v29i94295","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5104332251","display_name":"Rupam Sachan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rupam Sachan","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5147129192","display_name":"Suman Kumar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Suman Kumar","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056490008","display_name":"Rupesh Verma","orcid":"https://orcid.org/0000-0003-1151-6777","observed_orcids":["https://orcid.org/0000-0003-1151-6777"]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rupesh Verma","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077984741","display_name":"G. Das","orcid":"https://orcid.org/0000-0002-9664-7743","observed_orcids":["https://orcid.org/0000-0002-9664-7743"]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Giridhari Das","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025480744","display_name":"Subhradal Nath","orcid":"https://orcid.org/0000-0003-4789-5238","observed_orcids":["https://orcid.org/0000-0003-4789-5238"]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Subhradal Nath","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078671612","display_name":"Reetika Chourasia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Reetika Chourasia","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5147134693","display_name":"Hrithika Bhaumik","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Hrithika Bhaumik","raw_affiliation_strings":["Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Parasitology, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006308008","display_name":"Ravi Dabas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223471776","display_name":"Guru Angad Dev Veterinary and Animal Sciences University","ror":"https://ror.org/00bbeqy02","country_code":"IN","type":"education","lineage":["https://openalex.org/I223471776"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Ravi Dabas","raw_affiliation_strings":["Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab-141004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab-141004, India","institution_ids":["https://openalex.org/I223471776"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049820459","display_name":"Tanu Sharma","orcid":"https://orcid.org/0000-0002-8670-082X","observed_orcids":["https://orcid.org/0000-0002-8670-082X"]},"institutions":[{"id":"https://openalex.org/I4210156499","display_name":"Nanaji Deshmukh Veterinary Science University","ror":"https://ror.org/05vts7w23","country_code":"IN","type":"education","lineage":["https://openalex.org/I4210156499"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Tanu Sharma","raw_affiliation_strings":["Department of Veterinary Medicine, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, College of Veterinary Science, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, India","institution_ids":["https://openalex.org/I4210156499"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5147129192"],"corresponding_institution_ids":["https://openalex.org/I4210156499"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"29","issue":"9","first_page":"167","last_page":"173"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/anaplasma-marginale","display_name":"Anaplasma marginale","score":0.9990000128746033},{"id":"https://openalex.org/keywords/bovine-anaplasmosis","display_name":"bovine anaplasmosis","score":1.0},{"id":"https://openalex.org/keywords/carrier-cattle","display_name":"carrier cattle","score":0.800000011920929},{"id":"https://openalex.org/keywords/semi-nested-pcr","display_name":"semi-nested PCR","score":0.9350000023841858},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.8510000109672546},{"id":"https://openalex.org/keywords/cattle","display_name":"cattle","score":0.3540000021457672}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.664900004863739},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.6202999949455261},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5059999823570251},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.49380001425743103},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.42809998989105225},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35089999437332153},{"id":"https://openalex.org/C3017819844","wikidata":"https://www.wikidata.org/wiki/Q886475","display_name":"Blood smear","level":3,"score":0.3481999933719635},{"id":"https://openalex.org/C2908982167","wikidata":"https://www.wikidata.org/wiki/Q830","display_name":"Cattle Diseases","level":2,"score":0.34790000319480896},{"id":"https://openalex.org/C2777479583","wikidata":"https://www.wikidata.org/wiki/Q70288","display_name":"Anaplasma","level":3,"score":0.3303000032901764},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.31540000438690186},{"id":"https://openalex.org/C82157600","wikidata":"https://www.wikidata.org/wiki/Q2671652","display_name":"Diagnostic test","level":2,"score":0.3001999855041504},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.29420000314712524},{"id":"https://openalex.org/C45189115","wikidata":"https://www.wikidata.org/wiki/Q502159","display_name":"Serology","level":3,"score":0.29100000858306885},{"id":"https://openalex.org/C179786068","wikidata":"https://www.wikidata.org/wiki/Q16257937","display_name":"Molecular diagnostics","level":2,"score":0.2842000126838684},{"id":"https://openalex.org/C2987315675","wikidata":"https://www.wikidata.org/wiki/Q1773974","display_name":"Disease control","level":2,"score":0.2809000015258789},{"id":"https://openalex.org/C42972112","wikidata":"https://www.wikidata.org/wiki/Q170201","display_name":"Veterinary medicine","level":1,"score":0.259799987077713},{"id":"https://openalex.org/C157431215","wikidata":"https://www.wikidata.org/wiki/Q856465","display_name":"Nested polymerase chain reaction","level":4,"score":0.2535000145435333}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.9734/jabb/2026/v29i94295","doi":"https://doi.org/10.9734/jabb/2026/v29i94295","is_oa":true,"landing_page_url":"https://doi.org/10.9734/jabb/2026/v29i94295","pdf_url":null,"source":{"id":"https://openalex.org/S2764613355","display_name":"Journal of Advances in Biology & Biotechnology","issn_l":"2394-1081","issn":["2394-1081"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320855","host_organization_name":"Sciencedomain International","host_organization_lineage":["https://openalex.org/P4310320855"],"host_organization_lineage_names":["Sciencedomain International"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Biology & Biotechnology","raw_type":"journal-article"},{"id":"pmh:oai:journaljabb.com:article/4295","doi":null,"is_oa":true,"landing_page_url":"https://journaljabb.com/index.php/JABB/article/view/4295","pdf_url":null,"source":{"id":"https://openalex.org/S2764613355","display_name":"Journal of Advances in Biology & Biotechnology","issn_l":"2394-1081","issn":["2394-1081"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320855","host_organization_name":"Sciencedomain International","host_organization_lineage":["https://openalex.org/P4310320855"],"host_organization_lineage_names":["Sciencedomain International"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2394-1081","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:HAL:hal-05717324v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-05717324","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Advances in Biology & Biotechnology, 2026, 29 (9), pp.167-173","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.9734/jabb/2026/v29i94295","doi":"https://doi.org/10.9734/jabb/2026/v29i94295","is_oa":true,"landing_page_url":"https://doi.org/10.9734/jabb/2026/v29i94295","pdf_url":null,"source":{"id":"https://openalex.org/S2764613355","display_name":"Journal of Advances in Biology & Biotechnology","issn_l":"2394-1081","issn":["2394-1081"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320855","host_organization_name":"Sciencedomain International","host_organization_lineage":["https://openalex.org/P4310320855"],"host_organization_lineage_names":["Sciencedomain International"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Advances in Biology & Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2030954451","https://openalex.org/W3135786783","https://openalex.org/W2096106968","https://openalex.org/W4289716414","https://openalex.org/W1995383563","https://openalex.org/W3088209072","https://openalex.org/W3030341691","https://openalex.org/W2112651984","https://openalex.org/W4394790177","https://openalex.org/W1545707013"],"abstract_inverted_index":{"Bovine":[0],"anaplasmosis,":[1],"caused":[2],"by":[3,21,170],"Anaplasma":[4],"marginale,":[5],"is":[6,34,184],"a":[7,61,123,140,185],"major":[8,70],"tick-borne":[9],"haemoparasitic":[10],"disease":[11,38],"that":[12,167,178],"causes":[13],"significant":[14],"economic":[15],"losses":[16],"to":[17,48,57],"the":[18,69,77,116,127,134,147,179],"cattle":[19,96,199],"industry":[20],"reducing":[22],"productivity":[23],"and":[24,40,59,172,188,200,209],"increasing":[25],"mortality.":[26],"Early":[27],"detection":[28,79],"of":[29,80,89,104,115,126,133,146,212],"persistently":[30],"infected":[31],"carrier":[32,85,162,198],"animals":[33,163],"essential":[35],"for":[36,76,193,204],"effective":[37,210],"surveillance":[39],"control;":[41],"however,":[42],"conventional":[43],"diagnostic":[44,152,191],"methods":[45],"often":[46],"fail":[47],"identify":[49],"low-level":[50],"infections.":[51],"The":[52,154],"present":[53],"study":[54],"was":[55],"undertaken":[56],"develop":[58],"evaluate":[60],"semi-nested":[62,137,181],"polymerase":[63],"chain":[64],"reaction":[65],"(PCR)":[66],"assay":[67,155,183],"targeting":[68],"surface":[71],"protein":[72],"5":[73],"(msp5)":[74],"gene":[75,129],"sensitive":[78],"A.":[81,109,195],"marginale":[82,110,196],"infection":[83],"in":[84,97,111,130,143,159,197],"cattle.":[86],"A":[87],"total":[88],"31":[90],"blood":[91,106],"samples":[92],"were":[93,168],"collected":[94],"from":[95],"Jabalpur":[98],"district,":[99],"Madhya":[100],"Pradesh.":[101],"Microscopic":[102],"examination":[103],"Giemsa-stained":[105],"smears":[107],"detected":[108,157],"only":[112],"16.1%":[113],"(5/31)":[114],"samples.":[117],"In":[118],"contrast,":[119],"primary":[120,173],"PCR":[121,138,182],"amplified":[122,139],"458-bp":[124],"fragment":[125,142],"msp5":[128],"41.93%":[131],"(13/31)":[132],"samples,":[135,148],"whereas":[136],"345-bp":[141],"70.96%":[144],"(22/31)":[145],"demonstrating":[149],"substantially":[150],"higher":[151],"sensitivity.":[153],"successfully":[156],"infections":[158],"apparently":[160],"healthy":[161],"with":[164],"low":[165],"rickettsaemia":[166],"missed":[169],"microscopy":[171],"PCR.":[174],"These":[175],"findings":[176],"indicate":[177],"developed":[180],"rapid,":[186],"sensitive,":[187],"reliable":[189],"molecular":[190],"tool":[192],"detecting":[194],"has":[201],"considerable":[202],"potential":[203],"routine":[205],"diagnosis,":[206],"epidemiological":[207],"surveillance,":[208],"control":[211],"bovine":[213],"anaplasmosis.":[214]},"counts_by_year":[],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2026-08-14T00:00:00"}