{"id":"https://openalex.org/W72002177","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","title":"Engineering artificial splicing factors with designed specificities","display_name":"Engineering artificial splicing factors with designed specificities","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W72002177","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","mag":"72002177"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.23.1_supplement.662.5","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100672833","display_name":"Zefeng Wang","orcid":"https://orcid.org/0000-0002-6605-3637","observed_orcids":["https://orcid.org/0000-0002-6605-3637","https://orcid.org/0000-0001-9385-7393"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zefeng Wang","raw_affiliation_strings":["Dept of Pharmacology UNC Chapel Hill NC 27599 NC","Dept of Pharmacology, UNC, Chapel Hill, NC 27599 NC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept of Pharmacology UNC Chapel Hill NC 27599 NC","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Dept of Pharmacology, UNC, Chapel Hill, NC 27599 NC","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100672833","display_name":"Zefeng Wang","orcid":"https://orcid.org/0000-0002-6605-3637","observed_orcids":["https://orcid.org/0000-0002-6605-3637","https://orcid.org/0000-0001-9385-7393"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yang Wang","raw_affiliation_strings":["Dept of Pharmacology UNC Chapel Hill NC 27599 NC","Dept of Pharmacology, UNC, Chapel Hill, NC 27599 NC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept of Pharmacology UNC Chapel Hill NC 27599 NC","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Dept of Pharmacology, UNC, Chapel Hill, NC 27599 NC","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113881686","display_name":"C.-G. Cheong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cheom\u2010Gil Cheong","raw_affiliation_strings":["Biochemistry and Biophysics UNC Chapel hill NC","Lab of Structure Biology NIEHS/NIH Research Triangle Park NC","Biochemistry and Biophysics, UNC, Chapel hill, NC","Lab of Structure Biology, NIEHS/NIH, Research Triangle Park, NC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry and Biophysics UNC Chapel hill NC","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Lab of Structure Biology NIEHS/NIH Research Triangle Park NC","institution_ids":["https://openalex.org/I4210095140"]},{"raw_affiliation_string":"Biochemistry and Biophysics, UNC, Chapel hill, NC","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Lab of Structure Biology, NIEHS/NIH, Research Triangle Park, NC","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000438750","display_name":"Traci M. Tanaka Hall","orcid":"https://orcid.org/0000-0001-6166-3009","observed_orcids":["https://orcid.org/0000-0001-6166-3009"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210095140","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Traci M Hall","raw_affiliation_strings":["Lab of Structure Biology NIEHS/NIH Research Triangle Park NC","Lab of Structure Biology, NIEHS/NIH, Research Triangle Park, NC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lab of Structure Biology NIEHS/NIH Research Triangle Park NC","institution_ids":["https://openalex.org/I4210095140"]},{"raw_affiliation_string":"Lab of Structure Biology, NIEHS/NIH, Research Triangle Park, NC","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210095140"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00581234,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":0.9430000185966492},{"id":"https://openalex.org/keywords/sequence-specific-rna-binding","display_name":"sequence-specific RNA binding","score":0.7400000095367432},{"id":"https://openalex.org/keywords/bcl-x","display_name":"Bcl-x","score":0.7179999947547913},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9120000004768372},{"id":"https://openalex.org/keywords/chemosensitivity","display_name":"chemosensitivity","score":0.9350000023841858},{"id":"https://openalex.org/keywords/cancer-therapy","display_name":"cancer therapy","score":0.7910000085830688},{"id":"https://openalex.org/keywords/pre-mrna-splicing-regulation","display_name":"pre-mRNA splicing regulation","score":0.7699999809265137}],"concepts":[{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.898760974407196},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.7443555593490601},{"id":"https://openalex.org/C73758832","wikidata":"https://www.wikidata.org/wiki/Q7578564","display_name":"Splicing factor","level":5,"score":0.7198885083198547},{"id":"https://openalex.org/C42576000","wikidata":"https://www.wikidata.org/wiki/Q5420618","display_name":"Exonic splicing enhancer","level":5,"score":0.7184838652610779},{"id":"https://openalex.org/C94671646","wikidata":"https://www.wikidata.org/wiki/Q207551","display_name":"Intron","level":3,"score":0.6836309432983398},{"id":"https://openalex.org/C36823959","wikidata":"https://www.wikidata.org/wiki/Q373027","display_name":"Exon","level":3,"score":0.6739132404327393},{"id":"https://openalex.org/C166342232","wikidata":"https://www.wikidata.org/wiki/Q11936831","display_name":"Minigene","level":5,"score":0.6641519665718079},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5341208577156067},{"id":"https://openalex.org/C69551769","wikidata":"https://www.wikidata.org/wiki/Q899401","display_name":"Protein splicing","level":5,"score":0.49757078289985657},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4509909451007843},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.42781826853752136},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.424384742975235},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3525438904762268},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3236026167869568},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.30695515871047974}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.23.1_supplement.662.5","doi":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.23.1_supplement.662.5","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.428600013256073,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"display_name":"Good health and well-being","score":0.8299999833106995,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2009593381","https://openalex.org/W2111207584","https://openalex.org/W2156762274","https://openalex.org/W1997991041","https://openalex.org/W2133616621","https://openalex.org/W2030364440","https://openalex.org/W3012654451","https://openalex.org/W2082142998","https://openalex.org/W2021628982","https://openalex.org/W2152468181"],"abstract_inverted_index":{"The":[0,161],"majority":[1],"of":[2,42,56,62,83,92,126,133,152,169,173],"human":[3,63,100],"genes":[4],"undergo":[5],"alternative":[6,54,84,124],"splicing":[7,22,45,55,68,74,85,93,108,120,125,138,141,175],"to":[8,27,31,51,71,105,122,157,163],"produce":[9,72],"multiple":[10],"isoforms":[11],"with":[12,67,76,98],"distinct":[13],"functions.":[14],"This":[15,136],"process":[16],"is":[17,95],"tightly":[18],"regulated":[19],"by":[20,117],"trans\u2010acting":[21],"factors":[23,46,75,176],"that":[24,47,177],"bind":[25],"specifically":[26,52],"exons":[28],"or":[29,33],"introns":[30],"promote":[32],"inhibit":[34],"their":[35],"splicing.":[36],"Here":[37],"we":[38],"report":[39],"the":[40,127,143,165],"engineering":[41,118],"novel":[43],"artificial":[44,73,174],"can":[48,178],"be":[49],"designed":[50],"modulate":[53,106,123],"target":[57,132,179],"genes.":[58],"Sequence\u2010specific":[59],"RNA\u2010binding":[60],"domains":[61],"Pumilio1":[64,170],"were":[65],"fused":[66],"regulatory":[69],"motifs":[70],"diverse":[77],"activities":[78],"in":[79,86],"regulating":[80],"different":[81],"types":[82],"several":[87,153],"reporter":[88],"systems.":[89],"Because":[90],"disruption":[91],"regulation":[94],"closely":[96],"associated":[97],"various":[99],"diseases,":[101],"a":[102,119,131],"new":[103],"method":[104],"disease\u2010associated":[107],"events":[109],"provides":[110],"therapeutic":[111],"potential.":[112],"We":[113],"demonstrated":[114],"such":[115],"potential":[116],"factor":[121,139],"endogenous":[128],"Bcl\u2010x":[129],"gene,":[130],"anti\u2010cancer":[134],"therapy.":[135],"engineered":[137],"shifted":[140],"toward":[142],"pro\u2010apoptotic":[144],"Bcl\u2010xS":[145],"isoform,":[146],"promoted":[147],"apoptosis,":[148],"and":[149],"increased":[150],"chemosensitivity":[151],"cancer":[154],"cell":[155],"lines":[156],"common":[158],"anti\u2010tumor":[159],"drugs.":[160],"ability":[162],"design":[164],"RNA":[166],"sequence":[167],"specificity":[168],"permits":[171],"creation":[172],"virtually":[180],"any":[181],"pre\u2010mRNA.":[182]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}