{"id":"https://openalex.org/W7197033008","doi":"https://doi.org/10.64898/2026.08.05.742576","title":"\u03bcMap-uAA: Photocatalytic proximity labeling targeted with single-residue precision","display_name":"\u03bcMap-uAA: Photocatalytic proximity labeling targeted with single-residue precision","publication_year":2026,"publication_date":"2026-08-06","ids":{"openalex":"https://openalex.org/W7197033008","doi":"https://doi.org/10.64898/2026.08.05.742576"},"language":"en","primary_location":{"id":"doi:10.64898/2026.08.05.742576","doi":"https://doi.org/10.64898/2026.08.05.742576","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.05.742576","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.08.05.742576","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5031291325","display_name":"Min Sun Kang","orcid":"https://orcid.org/0000-0002-5516-6417","observed_orcids":["https://orcid.org/0000-0002-5516-6417"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Min Sun Kang","raw_affiliation_strings":["Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5145398299","display_name":"Chun Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chun Li","raw_affiliation_strings":["Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079418536","display_name":"Benito F. Buksh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benito F. Buksh","raw_affiliation_strings":["Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5145679998","display_name":"David Fernandez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David F. Fern\u00e1ndez","raw_affiliation_strings":["Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025805979","display_name":"David W. C. MacMillan","orcid":"https://orcid.org/0000-0001-6447-0587","observed_orcids":["https://orcid.org/0000-0001-6447-0587"]},"institutions":[{"id":"https://openalex.org/I196064397","display_name":"Ludwig Cancer Research","ror":"https://ror.org/05923xh51","country_code":"BE","type":"nonprofit","lineage":["https://openalex.org/I196064397","https://openalex.org/I4210150094"]},{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["BE","US"],"is_corresponding":true,"raw_author_name":"David W. C. MacMillan","raw_affiliation_strings":["Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","Ludwig Institute for Cancer Research, Princeton Branch, Princeton University, Princeton, NJ 08544, USA","Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA"],"raw_orcid":"https://orcid.org/0000-0001-6447-0587","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]},{"raw_affiliation_string":"Ludwig Institute for Cancer Research, Princeton Branch, Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I196064397","https://openalex.org/I20089843"]},{"raw_affiliation_string":"Merck Center for Catalysis at Princeton University, Princeton, NJ 08544, USA","institution_ids":["https://openalex.org/I20089843"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5025805979"],"corresponding_institution_ids":["https://openalex.org/I196064397","https://openalex.org/I20089843"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proximity-labeling","display_name":"proximity labeling","score":0.6320000290870667},{"id":"https://openalex.org/keywords/unnatural-amino-acid-incorporation","display_name":"unnatural amino acid incorporation","score":0.8069999814033508},{"id":"https://openalex.org/keywords/photocatalysis","display_name":"photocatalysis","score":0.843999981880188},{"id":"https://openalex.org/keywords/iridium-photocatalyst","display_name":"iridium photocatalyst","score":0.9010000228881836},{"id":"https://openalex.org/keywords/click-chemistry","display_name":"click chemistry","score":0.9549999833106995},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.8180000185966492},{"id":"https://openalex.org/keywords/cell-surface-receptors","display_name":"cell surface receptors","score":0.8820000290870667},{"id":"https://openalex.org/keywords/living-cells","display_name":"living cells","score":0.4429999887943268}],"concepts":[{"id":"https://openalex.org/C65165184","wikidata":"https://www.wikidata.org/wiki/Q218831","display_name":"Photocatalysis","level":3,"score":0.7544999718666077},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5845999717712402},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.5665000081062317},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.519599974155426},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.43970000743865967},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.4352000057697296},{"id":"https://openalex.org/C528581852","wikidata":"https://www.wikidata.org/wiki/Q877","display_name":"Iridium","level":3,"score":0.4323999881744385},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4318000078201294},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.42899999022483826},{"id":"https://openalex.org/C2994376932","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Amino acid residue","level":4,"score":0.40610000491142273},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.3840999901294708},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.361299991607666},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.35760000348091125},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3418999910354614},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.30070000886917114},{"id":"https://openalex.org/C52909226","wikidata":"https://www.wikidata.org/wiki/Q5462835","display_name":"Fluorescent labelling","level":3,"score":0.28439998626708984},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.2793999910354614},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.2768000066280365},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.27250000834465027},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.26669999957084656},{"id":"https://openalex.org/C66746571","wikidata":"https://www.wikidata.org/wiki/Q1134833","display_name":"ENCODE","level":3,"score":0.26179999113082886},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.2522999942302704},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.25110000371932983}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.08.05.742576","doi":"https://doi.org/10.64898/2026.08.05.742576","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.05.742576","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.08.05.742576","doi":"https://doi.org/10.64898/2026.08.05.742576","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.08.05.742576","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1955176809","https://openalex.org/W1974051196","https://openalex.org/W1982899294","https://openalex.org/W1996183144","https://openalex.org/W1999048558","https://openalex.org/W1999417836","https://openalex.org/W2079470893","https://openalex.org/W2116185344","https://openalex.org/W2131457893","https://openalex.org/W2163485494","https://openalex.org/W2472183924","https://openalex.org/W2519031788","https://openalex.org/W2593841038","https://openalex.org/W2743448525","https://openalex.org/W2780020423","https://openalex.org/W3010256122","https://openalex.org/W3010320439","https://openalex.org/W3045755423","https://openalex.org/W3208579898","https://openalex.org/W4255761019","https://openalex.org/W4292415248","https://openalex.org/W4312065609","https://openalex.org/W4399970359","https://openalex.org/W4402057714","https://openalex.org/W4402193849","https://openalex.org/W4404259754","https://openalex.org/W4408121828","https://openalex.org/W4409787462"],"related_works":["https://openalex.org/W4385347793","https://openalex.org/W3108419245","https://openalex.org/W4384925259","https://openalex.org/W4312144113","https://openalex.org/W7163000820","https://openalex.org/W7153119164","https://openalex.org/W4220987448","https://openalex.org/W4319815224","https://openalex.org/W3160430706","https://openalex.org/W3040591885"],"abstract_inverted_index":{"ABSTRACT":[0],"Mapping":[1],"protein":[2,35],"microenvironments":[3],"with":[4,69],"residue-level":[5,70],"precision":[6],"in":[7],"living":[8],"cells":[9],"remains":[10],"challenging.":[11],"We":[12],"report":[13],"\u03bcMap-uAA,":[14],"a":[15,29],"genetically":[16],"encoded":[17],"proximity":[18],"labeling":[19,53],"platform":[20],"that":[21],"uses":[22],"unnatural":[23],"amino":[24],"acid":[25],"incorporation":[26],"to":[27,46,58],"install":[28],"tetrazine-quenched":[30],"iridium":[31],"photocatalyst":[32],"at":[33],"user-defined":[34],"sites":[36],"through":[37],"click":[38],"chemistry.":[39],"Photocatalysis":[40],"is":[41],"activated":[42],"by":[43],"covalent":[44],"attachment":[45],"the":[47],"incorporated":[48],"uAA,":[49],"enabling":[50],"localized":[51],"catalytic":[52],"of":[54],"proximal":[55],"biomolecules.":[56],"Applied":[57],"membrane":[59],"receptors,":[60],"\u03bcMap-uAA":[61],"captures":[62],"domain-":[63],"and":[64],"time-dependent":[65],"GPCR":[66],"interaction":[67],"networks":[68],"spatial":[71],"precision.":[72]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-08-07T00:00:00"}