{"id":"https://openalex.org/W71855877","doi":null,"title":"Purification, characterisation and steady state kinetic properties of cytosolic pyruvate kinase free of phosphoenol pyruvate phosphatase activity from germinating mung beans (Vigna radiata L.)","display_name":"Purification, characterisation and steady state kinetic properties of cytosolic pyruvate kinase free of phosphoenol pyruvate phosphatase activity from germinating mung beans (Vigna radiata L.)","publication_year":1996,"publication_date":"1996-06-01","ids":{"openalex":"https://openalex.org/W71855877","mag":"71855877","pmid":"https://pubmed.ncbi.nlm.nih.gov/8828288"},"language":"en","primary_location":{"id":"pmid:8828288","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8828288","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian journal of biochemistry & biophysics","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044927019","display_name":"Pravin Kumar Ambasht","orcid":"https://orcid.org/0000-0001-9425-4762","observed_orcids":["https://orcid.org/0000-0001-9425-4762"]},"institutions":[{"id":"https://openalex.org/I91357014","display_name":"Banaras Hindu University","ror":"https://ror.org/04cdn2797","country_code":"IN","type":"education","lineage":["https://openalex.org/I91357014"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"P K Ambasht","raw_affiliation_strings":["Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, India","institution_ids":["https://openalex.org/I91357014"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112017154","display_name":"O.P. Malhotra","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"O P Malhotra","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5007531872","display_name":"Arvind M. Kayastha","orcid":"https://orcid.org/0000-0002-5090-7159","observed_orcids":["https://orcid.org/0000-0002-5090-7159"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A M Kayastha","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.0026494,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"33","issue":"3","first_page":"184","last_page":"94"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5454000234603882,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5454000234603882,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.1720000058412552,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.054099999368190765,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pyruvate-kinase","display_name":"pyruvate kinase","score":0.9419999718666077},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8119999766349792},{"id":"https://openalex.org/keywords/mungbean","display_name":"mungbean","score":0.9879999756813049},{"id":"https://openalex.org/keywords/enzyme-inactivation","display_name":"enzyme inactivation","score":0.6840000152587891},{"id":"https://openalex.org/keywords/magnesium-requirement","display_name":"magnesium requirement","score":0.36899998784065247},{"id":"https://openalex.org/keywords/manganese-requirement","display_name":"manganese requirement","score":0.781000018119812},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.421999990940094},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.6439999938011169},{"id":"https://openalex.org/keywords/atp-inhibition","display_name":"ATP inhibition","score":0.6209999918937683},{"id":"https://openalex.org/keywords/ternary-complex-formation","display_name":"ternary complex formation","score":0.7620000243186951},{"id":"https://openalex.org/keywords/germination","display_name":"germination","score":0.3659999966621399}],"concepts":[{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6688621044158936},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6553084254264832},{"id":"https://openalex.org/C2779862049","wikidata":"https://www.wikidata.org/wiki/Q132634","display_name":"Vigna","level":2,"score":0.6500024795532227},{"id":"https://openalex.org/C2780854706","wikidata":"https://www.wikidata.org/wiki/Q900970","display_name":"Pyruvate kinase","level":4,"score":0.5370017886161804},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5069432258605957},{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.5053822994232178},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5045963525772095},{"id":"https://openalex.org/C181912034","wikidata":"https://www.wikidata.org/wiki/Q1101143","display_name":"Dephosphorylation","level":4,"score":0.4113219380378723},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.15044957399368286},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.07327991724014282},{"id":"https://openalex.org/C20251656","wikidata":"https://www.wikidata.org/wiki/Q162643","display_name":"Glycolysis","level":3,"score":0.06917238235473633},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000135","descriptor_name":"Acid Phosphatase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000645","descriptor_name":"Ammonium Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002413","descriptor_name":"Cations, Divalent","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002848","descriptor_name":"Chromatography, DEAE-Cellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007887","descriptor_name":"Fabaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010946","descriptor_name":"Plants, Medicinal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011770","descriptor_name":"Pyruvate Kinase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011770","descriptor_name":"Pyruvate Kinase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011770","descriptor_name":"Pyruvate Kinase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8828288","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8828288","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian journal of biochemistry & biophysics","raw_type":"Journal Article"},{"id":"mag:71855877","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8828288","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2012279918","https://openalex.org/W2068809338","https://openalex.org/W2468268396","https://openalex.org/W1908430680","https://openalex.org/W1998056619","https://openalex.org/W1970889686","https://openalex.org/W2092693884","https://openalex.org/W2146089140","https://openalex.org/W2066892342","https://openalex.org/W2044135391"],"abstract_inverted_index":{"Mung":[0,60],"bean":[1,58,61],"pyruvate":[2],"kinase":[3],"(PK)":[4],"practically":[5],"free":[6],"from":[7],"PEP-phosphatase":[8],"has":[9,63],"been":[10],"purified":[11],"about":[12],"36":[13],"fold.":[14],"The":[15,29,85,112,136,141],"enzyme":[16,105,142,166],"is":[17,31,43,106,139],"irreversibly":[18],"inactivated":[19],"on":[20,212],"desalting":[21],"by":[22,45,202],"gel":[23],"filtration":[24],"or":[25,40,152,172],"dialysis":[26],"(without":[27],"EDTA).":[28],"inactivation":[30],"also":[32],"observed":[33],"in":[34,55,167],"the":[35,56,104,109,165,168,195,213],"presence":[36,169],"of":[37,67,75,87,170,192,197,205],"ATP,":[38],"Mg2+":[39,148,171],"thiols":[41],"but":[42,163],"prevented":[44],"a":[46,64,198,203],"non-proteinous,":[47],"heat":[48],"stable,":[49],"small":[50],"molecular":[51,65],"mass":[52,66],"factor":[53],"present":[54],"mung":[57],"extract.":[59],"PK":[62],"210":[68],"kDa.":[69],"It":[70],"shows":[71,143,176],"single":[72],"exponential":[73],"decay":[74],"activity":[76],"at":[77,100,124,133,187],"various":[78],"temperatures":[79],"(-4":[80],"to":[81,93],"60":[82],"degrees":[83],"C).":[84],"Km":[86,113],"PEP":[88,116],"and":[89,96,117,121,127,129,181,190,207],"ADP":[90,118,180],"are":[91,119,160],"found":[92],"be":[94],"0.12":[95],"0.24":[97],"mM,":[98,123,131],"respectively":[99,132],"pH":[101,125,134,138],"6.5,":[102],"when":[103],"saturated":[107],"with":[108,179,183],"second":[110],"substrate.":[111],"values":[114],"for":[115,147],"0.05":[120],"0.16":[122],"8.5":[126],"0.09":[128],"0.17":[130],"7.5.":[135,140],"optimum":[137],"an":[144],"absolute":[145],"requirement":[146],"(Km":[149,155],"0.43":[150],"mM)":[151],"Mn2+":[153,173],"ions":[154,159],"0.125":[156],"mM).":[157],"Potassium":[158],"not":[161],"essential":[162],"activate":[164],"ions.":[174],"ATP":[175,193],"competitive":[177],"inhibition":[178],"non-competitive":[182],"PEP.":[184],"Kinetic":[185],"studies":[186],"different":[188],"pHs":[189],"effects":[191],"suggest":[194],"formation":[196],"ternary":[199],"complex":[200],"(E.ADP.PEP)":[201],"combination":[204],"random":[206],"compulsory":[208],"ordered":[209],"pathways":[210],"depending":[211],"experimental":[214],"conditions.":[215]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}