{"id":"https://openalex.org/W7171534029","doi":"https://doi.org/10.1093/jimmun/vkag141.1608","title":"Multiplex Made Practical: An Educational Workflow Using Directly Conjugated Primary Antibodies 2309766","display_name":"Multiplex Made Practical: An Educational Workflow Using Directly Conjugated Primary Antibodies 2309766","publication_year":2026,"publication_date":"2026-07-28","ids":{"openalex":"https://openalex.org/W7171534029","doi":"https://doi.org/10.1093/jimmun/vkag141.1608"},"language":"en","primary_location":{"id":"doi:10.1093/jimmun/vkag141.1608","doi":"https://doi.org/10.1093/jimmun/vkag141.1608","is_oa":false,"landing_page_url":"https://doi.org/10.1093/jimmun/vkag141.1608","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-2","ki-jl-2","medline","norway-2"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5094223231","display_name":"Jen Serafin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jen Serafin","raw_affiliation_strings":["Thermo Fisher Scientific, Inc"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thermo Fisher Scientific, Inc","institution_ids":["https://openalex.org/I4210142480"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5094223232","display_name":"Mae Voeun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210142480","display_name":"Thermo Fisher Scientific (United States)","ror":"https://ror.org/03x1ewr52","country_code":"US","type":"company","lineage":["https://openalex.org/I4210142480"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mae Voeun","raw_affiliation_strings":["Thermo Fisher Scientific"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thermo Fisher Scientific","institution_ids":["https://openalex.org/I4210142480"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210142480"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.5780932,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"215","issue":"Supplement_1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.8003000020980835,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.08860000222921371,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.020600000396370888,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spatial-multiplexing","display_name":"spatial multiplexing","score":0.6259999871253967},{"id":"https://openalex.org/keywords/antibody-labeling","display_name":"antibody labeling","score":0.5360000133514404},{"id":"https://openalex.org/keywords/colon-cancer","display_name":"colon cancer","score":0.6909999847412109},{"id":"https://openalex.org/keywords/colorectal-adenocarcinoma","display_name":"colorectal adenocarcinoma","score":0.9340000152587891},{"id":"https://openalex.org/keywords/tumor-immune-microenvironment","display_name":"tumor immune microenvironment","score":0.8579999804496765},{"id":"https://openalex.org/keywords/tertiary-lymphoid-structures","display_name":"tertiary lymphoid structures","score":0.9430000185966492},{"id":"https://openalex.org/keywords/regulatory-t-cells","display_name":"regulatory T cells","score":0.8799999952316284},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.7739999890327454},{"id":"https://openalex.org/keywords/pd-1","display_name":"PD-1","score":0.6830000281333923},{"id":"https://openalex.org/keywords/cd3","display_name":"CD3","score":0.5440000295639038},{"id":"https://openalex.org/keywords/podoplanin","display_name":"podoplanin","score":0.6759999990463257},{"id":"https://openalex.org/keywords/photobleaching","display_name":"photobleaching","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.8112000226974487},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.6054999828338623},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.5824000239372253},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5651999711990356},{"id":"https://openalex.org/C21269302","wikidata":"https://www.wikidata.org/wiki/Q7243098","display_name":"Primary and secondary antibodies","level":3,"score":0.5297999978065491},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.48539999127388},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.474700003862381},{"id":"https://openalex.org/C2779998722","wikidata":"https://www.wikidata.org/wiki/Q420296","display_name":"Cetuximab","level":4,"score":0.3537999987602234},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3224000036716461},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.29319998621940613},{"id":"https://openalex.org/C2909234372","wikidata":"https://www.wikidata.org/wiki/Q188874","display_name":"Colorectal adenocarcinoma","level":4,"score":0.27900001406669617},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.2685999870300293},{"id":"https://openalex.org/C114684123","wikidata":"https://www.wikidata.org/wiki/Q419458","display_name":"CD3","level":4,"score":0.26809999346733093},{"id":"https://openalex.org/C2779256057","wikidata":"https://www.wikidata.org/wiki/Q16909647","display_name":"Tumor progression","level":3,"score":0.26649999618530273},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.2619999945163727}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1093/jimmun/vkag141.1608","doi":"https://doi.org/10.1093/jimmun/vkag141.1608","is_oa":false,"landing_page_url":"https://doi.org/10.1093/jimmun/vkag141.1608","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-2","ki-jl-2","medline","norway-2"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.5232334136962891,"display_name":"No poverty","id":"https://metadata.un.org/sdg/1"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Introduction":[1],"Spatial":[2],"biology":[3],"multiplex":[4,230],"panels":[5,49,231],"built":[6],"from":[7],"directly":[8,82,228],"conjugated":[9,98,229],"primary":[10,47],"antibodies":[11,84],"accelerate":[12,251],"staining":[13],"workflows":[14,244],"and":[15,37,45,53,58,69,94,105,131,163,175,192,217,254,280],"improve":[16],"quantification":[17],"accuracy":[18],"of":[19,67,111,125,149,213],"immunomarker":[20],"expression":[21],"by":[22],"eliminating":[23],"amplification":[24],"bias.":[25],"These":[26,224],"advantages":[27],"allow":[28],"scientists":[29],"to":[30,43,50,85,119,134,237,250],"interrogate":[31],"complex":[32,239],"tissues":[33],"with":[34,155,172],"greater":[35],"depth":[36],"precision.":[38],"Here,":[39],"we":[40,72,77,106,182],"outline":[41],"how":[42,207],"create":[44],"optimize":[46],"antibody":[48,102],"characterize":[51],"immune":[52,201,214],"structural":[54],"features":[55,241],"in":[56,75,152,161,167,190,268],"healthy":[57],"malignant":[59],"colon":[60],"tissues.":[61],"Methods":[62],"Using":[63],"a":[64,79,123,158,164,184,197,233],"practical":[65],"framework":[66],"steps":[68],"considerations":[70],"that":[71,227,245],"will":[73,107],"highlight":[74,132,206],"detail,":[76],"designed":[78],"panel":[80],"comprising":[81],"labeled":[83],"podoplanin,":[86],"CD4,":[87],"FoxP3,":[88],"Ki67,":[89],"CD8,":[90],"CD68,":[91],"PD":[92],"1,":[93],"CD3.":[95],"CD3":[96],"was":[97],"in-house":[99],"using":[100,122,139],"an":[101,116],"labeling":[103],"kit,":[104],"describe":[108,115],"the":[109,179],"optimization":[110],"this":[112],"procedure.":[113],"We":[114],"effective":[117],"method":[118],"combat":[120],"autofluorescence":[121],"combination":[124],"white":[126],"light":[127],"photobleaching":[128],"+":[129],"H2O2":[130],"ways":[133],"achieve":[135],"superior":[136],"unmixing":[137],"performance":[138],"EVOS":[140],"S1000.":[141],"Results":[142],"Immune":[143],"cells":[144,151],"comprised":[145],"only":[146],"about":[147],"10%":[148],"total":[150],"both":[153,170],"samples,":[154],"tumors":[156],"showing":[157],"marked":[159],"increase":[160],"macrophages":[162],"dramatic":[165],"rise":[166],"proliferating":[168],"Tregs,":[169],"associated":[171],"tumor":[173],"progression":[174],"poorer":[176],"outcomes.":[177],"In":[178],"adenocarcinoma":[180],"sample,":[181],"identified":[183],"tertiary":[185],"lymphoid":[186],"structure":[187],"enriched":[188],"primarily":[189],"cytotoxic":[191],"helper":[193],"T":[194],"cells,":[195],"suggesting":[196],"potentially":[198],"beneficial":[199],"anti-tumor":[200],"niche.":[202],"Overall,":[203],"these":[204],"findings":[205,225],"high-plex":[208],"imaging":[209],"enables":[210],"detailed":[211],"characterization":[212],"cell":[215],"composition":[216],"spatial":[218,248],"organization":[219],"within":[220],"colorectal":[221],"tumors.":[222],"Conclusion":[223],"show":[226],"offer":[232],"robust,":[234],"scalable":[235],"way":[236],"resolve":[238],"tissue":[240],"while":[242],"providing":[243],"generate":[246],"high-quality":[247],"data":[249],"biological":[252],"insight":[253],"discovery.":[255],"All":[256],"reagents":[257],"are":[258],"intended":[259],"for":[260,266],"Research":[261],"Use":[262],"Only":[263],"(RUO).":[264],"Not":[265],"use":[267],"diagnostic":[269],"procedures.":[270],"Funding":[271],"Source":[272],"N/A":[273],"Topic":[274],"Categories":[275],"Tumor":[276,281],"Immunology:":[277],"Cellular":[278],"Responses":[279],"Microevironment":[282],"(TIME)":[283]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2026-07-29T00:00:00"}
