{"id":"https://openalex.org/W7170147178","doi":"https://doi.org/10.64898/2026.07.22.740104","title":"PROPEL: a high-throughput shear-stress platform reveals organotypic thresholds in endothelial mechano-adaptation","display_name":"PROPEL: a high-throughput shear-stress platform reveals organotypic thresholds in endothelial mechano-adaptation","publication_year":2026,"publication_date":"2026-07-23","ids":{"openalex":"https://openalex.org/W7170147178","doi":"https://doi.org/10.64898/2026.07.22.740104"},"language":"en","primary_location":{"id":"doi:10.64898/2026.07.22.740104","doi":"https://doi.org/10.64898/2026.07.22.740104","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.22.740104","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/23/2026.07.22.740104.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/23/2026.07.22.740104.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5143523473","display_name":"Terry Ching","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I44142251","display_name":"Wyss Institute for Biologically Inspired Engineering","ror":"https://ror.org/008cfmj78","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I44142251"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Terry Ching","raw_affiliation_strings":["Boston University; Wyss Institute at Harvard;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston University; Wyss Institute at Harvard;","institution_ids":["https://openalex.org/I111088046","https://openalex.org/I136199984","https://openalex.org/I44142251"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069434821","display_name":"Jessica L. Teo","orcid":"https://orcid.org/0000-0002-8171-4763","observed_orcids":["https://orcid.org/0000-0002-8171-4763"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I44142251","display_name":"Wyss Institute for Biologically Inspired Engineering","ror":"https://ror.org/008cfmj78","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I44142251"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica Teo","raw_affiliation_strings":["Boston University; Wyss Institute at Harvard;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston University; Wyss Institute at Harvard;","institution_ids":["https://openalex.org/I111088046","https://openalex.org/I136199984","https://openalex.org/I44142251"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5116545936","display_name":"Winita Wangsrikhun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Winita Wangsrikhun","raw_affiliation_strings":["Boston University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston University","institution_ids":["https://openalex.org/I111088046"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090414812","display_name":"Xiaolei Song","orcid":"https://orcid.org/0000-0002-0206-1574","observed_orcids":["https://orcid.org/0000-0002-0206-1574"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaolei Song","raw_affiliation_strings":["Boston University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston University","institution_ids":["https://openalex.org/I111088046"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100609547","display_name":"Christopher S. Chen","orcid":"https://orcid.org/0000-0003-2445-8449","observed_orcids":["https://orcid.org/0000-0003-2445-8449"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I44142251","display_name":"Wyss Institute for Biologically Inspired Engineering","ror":"https://ror.org/008cfmj78","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I44142251"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christopher S Chen","raw_affiliation_strings":["Boston University; Wyss Institute at Harvard;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Boston University; Wyss Institute at Harvard;","institution_ids":["https://openalex.org/I111088046","https://openalex.org/I136199984","https://openalex.org/I44142251"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5100609547"],"corresponding_institution_ids":["https://openalex.org/I111088046","https://openalex.org/I136199984","https://openalex.org/I44142251"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/endothelial-cells","display_name":"endothelial cells","score":0.8529999852180481},{"id":"https://openalex.org/keywords/hemodynamic-shear-stress","display_name":"hemodynamic shear stress","score":0.8119999766349792},{"id":"https://openalex.org/keywords/mechanotransduction","display_name":"mechanotransduction","score":0.8019999861717224},{"id":"https://openalex.org/keywords/cell-alignment","display_name":"cell alignment","score":0.7329999804496765},{"id":"https://openalex.org/keywords/cell-elongation","display_name":"cell elongation","score":0.9399999976158142},{"id":"https://openalex.org/keywords/dermal-microvascular-endothelial-cells","display_name":"dermal microvascular endothelial cells","score":0.859000027179718},{"id":"https://openalex.org/keywords/critical-shear-stress","display_name":"critical shear stress","score":0.5189999938011169},{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.6389999985694885},{"id":"https://openalex.org/keywords/metabolic-response","display_name":"metabolic response","score":0.6200000047683716}],"concepts":[{"id":"https://openalex.org/C181104049","wikidata":"https://www.wikidata.org/wiki/Q11936292","display_name":"Mechanotransduction","level":2,"score":0.6859999895095825},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6805999875068665},{"id":"https://openalex.org/C21141959","wikidata":"https://www.wikidata.org/wiki/Q657936","display_name":"Shear stress","level":2,"score":0.513700008392334},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45969998836517334},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.45660001039505005},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.4505000114440918},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.4320000112056732},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.3831000030040741},{"id":"https://openalex.org/C2776992346","wikidata":"https://www.wikidata.org/wiki/Q111140","display_name":"Endothelium","level":2,"score":0.3797000050544739},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.350600004196167},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.323199987411499},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.28519999980926514},{"id":"https://openalex.org/C39743133","wikidata":"https://www.wikidata.org/wiki/Q3262595","display_name":"Signalling","level":2,"score":0.2851000130176544},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.27619999647140503},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.2703000009059906},{"id":"https://openalex.org/C189014844","wikidata":"https://www.wikidata.org/wiki/Q189118","display_name":"Cell type","level":3,"score":0.2694000005722046},{"id":"https://openalex.org/C7876069","wikidata":"https://www.wikidata.org/wiki/Q14873666","display_name":"Paracrine signalling","level":3,"score":0.2621999979019165},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.259799987077713},{"id":"https://openalex.org/C33947775","wikidata":"https://www.wikidata.org/wiki/Q271439","display_name":"Elongation","level":3,"score":0.25850000977516174},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.2524000108242035}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.07.22.740104","doi":"https://doi.org/10.64898/2026.07.22.740104","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.22.740104","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/23/2026.07.22.740104.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.07.22.740104","doi":"https://doi.org/10.64898/2026.07.22.740104","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.22.740104","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/23/2026.07.22.740104.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1199548893","display_name":null,"funder_award_id":"CMMI 1548571","funder_id":"https://openalex.org/F4320337391","funder_display_name":"Division of Civil, Mechanical and Manufacturing Innovation"},{"id":"https://openalex.org/G1870677656","display_name":null,"funder_award_id":"CMMI: 15\u201048571","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G4481177325","display_name":null,"funder_award_id":"15\u201348571","funder_id":"https://openalex.org/F4320337391","funder_display_name":"Division of Civil, Mechanical and Manufacturing Innovation"},{"id":"https://openalex.org/G6717099065","display_name":null,"funder_award_id":"1548571","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6956364634","display_name":"Investigating flow-induced Notch-activated vascular barrier function using SynNotch receptors","funder_award_id":"24POST1243689","funder_id":"https://openalex.org/F4320306230","funder_display_name":"American Heart Association"},{"id":"https://openalex.org/G730080929","display_name":"Local Regulation of Angiogenesis by Microenvironment","funder_award_id":"R01EB000262","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G97630198","display_name":"Science and Technology Center for Engineering Mechano-Biology","funder_award_id":"1548571","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320333979","display_name":"Hansj\u00f6rg Wyss Institute for Biologically Inspired Engineering, Harvard University","ror":"https://ror.org/008cfmj78"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"},{"id":"https://openalex.org/F4320337391","display_name":"Division of Civil, Mechanical and Manufacturing Innovation","ror":"https://ror.org/028yd4c30"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7170147178.pdf","grobid_xml":"https://content.openalex.org/works/W7170147178.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2110919322","https://openalex.org/W1977383165","https://openalex.org/W7172393026","https://openalex.org/W2020331803","https://openalex.org/W2038854702","https://openalex.org/W2763146615","https://openalex.org/W3087570052","https://openalex.org/W1979397042","https://openalex.org/W2029330485","https://openalex.org/W4410555974"],"abstract_inverted_index":{"Endothelial":[0],"cells":[1,112,183],"exhibit":[2],"organotypic":[3,189],"specialization,":[4],"yet":[5],"the":[6,50,58,206],"tools":[7],"to":[8,38,43,61,146,155],"decode":[9],"whether":[10],"hemodynamic":[11],"shear":[12,35,127,185,207],"shapes":[13],"this":[14,72],"diversity":[15],"across":[16,80],"vascular":[17,214],"beds":[18],"have":[19],"remained":[20],"limited.":[21],"Here":[22],"we":[23,74],"present":[24],"PROPEL,":[25],"a":[26,53,99,169,192,197,201],"tubing-free,":[27],"magnetic":[28],"stirrer-driven":[29],"platform":[30,171],"that":[31,123,181],"delivers":[32],"programmable":[33],"laminar":[34],"stress":[36,208],"(2":[37],"60":[39],"dyn/cm":[40],"2":[41],")":[42],"multiple":[44],"cell":[45],"types":[46],"in":[47,105],"parallel":[48],"within":[49],"confines":[51],"of":[52,131,136,148],"standard":[54],"Petri":[55],"dish,":[56],"with":[57,126,211],"modular":[59],"flexibility":[60],"easily":[62],"introduce":[63],"different":[64],"substrate":[65],"geometries":[66],"including":[67,98,129],"3D":[68],"vessel":[69],"formats.":[70],"Using":[71],"platform,":[73],"profiled":[75],"six":[76],"human":[77],"endothelial":[78,111,182],"subtypes":[79],"static,":[81],"low,":[82],"intermediate,":[83],"and":[84,95,103,108,150,176,179,200],"high":[85],"shear.":[86,115],"Phenotypic":[87],"profiling":[88],"revealed":[89],"subtype-specific":[90],"thresholds":[91],"for":[92,172,213],"alignment,":[93],"elongation,":[94],"Golgi-nuclear":[96],"polarization,":[97],"dissociation":[100],"between":[101],"elongation":[102],"polarization":[104],"dermal":[106],"microvascular":[107],"saphenous":[109],"venous":[110],"at":[113],"low":[114],"Bulk":[116],"RNA":[117],"sequencing":[118],"showed":[119],"conserved":[120,156],"transcriptional":[121,140],"programs":[122],"shift":[124],"progressively":[125],"magnitude,":[128],"induction":[130],"mechanotransduction":[132],"pathways":[133],"alongside":[134],"suppression":[135],"proliferative":[137],"programs.":[138],"Phenotype-informed":[139],"comparisons":[141],"further":[142],"linked":[143],"alignment":[144],"transitions":[145],"engagement":[147],"cytoskeletal":[149],"metabolic":[151],"signatures.":[152],"In":[153],"addition":[154],"responses,":[157],"organotypic-specific":[158],"responses":[159],"were":[160],"also":[161],"observed.":[162],"Together,":[163],"these":[164],"findings":[165],"establish":[166],"PROPEL":[167],"as":[168],"scalable":[170],"multi-endothelial,":[173],"multi-shear":[174],"transcriptomic":[175],"phenotypic":[177],"profiling,":[178],"reveal":[180],"engage":[184],"adaptation":[186],"along":[187],"two":[188],"axes":[190],"-":[191,210],"threshold":[193],"axis":[194,203],"governing":[195,204],"when":[196],"subtype":[198],"responds":[199],"signature":[202],"which":[205],"response":[209],"implications":[212],"bed-specific":[215],"disease":[216],"susceptibility.":[217]},"counts_by_year":[],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2026-07-24T00:00:00"}
