{"id":"https://openalex.org/W7167840109","doi":"https://doi.org/10.64898/2026.07.08.737154","title":"Molecular Alterations of Bovine Serum Albumin Induced by the Food Dye Acid Yellow 23: A Mechanistic Study","display_name":"Molecular Alterations of Bovine Serum Albumin Induced by the Food Dye Acid Yellow 23: A Mechanistic Study","publication_year":2026,"publication_date":"2026-07-09","ids":{"openalex":"https://openalex.org/W7167840109","doi":"https://doi.org/10.64898/2026.07.08.737154"},"language":"en","primary_location":{"id":"doi:10.64898/2026.07.08.737154","doi":"https://doi.org/10.64898/2026.07.08.737154","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.08.737154","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/09/2026.07.08.737154.1.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/09/2026.07.08.737154.1.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023105519","display_name":"Parshant Dahiya","orcid":"https://orcid.org/0000-0003-1366-1230","observed_orcids":["https://orcid.org/0000-0003-1366-1230"]},"institutions":[{"id":"https://openalex.org/I1331084196","display_name":"National Forensic Sciences University","ror":"https://ror.org/03fbv3w59","country_code":"IN","type":"education","lineage":["https://openalex.org/I1331084196"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Parshant Dahiya","raw_affiliation_strings":["School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat"],"raw_orcid":"https://orcid.org/0000-0003-1366-1230","affiliations":[{"raw_affiliation_string":"School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat","institution_ids":["https://openalex.org/I1331084196"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5140342608","display_name":"Alpana Verma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1331084196","display_name":"National Forensic Sciences University","ror":"https://ror.org/03fbv3w59","country_code":"IN","type":"education","lineage":["https://openalex.org/I1331084196"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Alpana Verma","raw_affiliation_strings":["School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat","institution_ids":["https://openalex.org/I1331084196"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037955145","display_name":"Vishal Arvindlal Mevada","orcid":"https://orcid.org/0000-0003-0739-026X","observed_orcids":["https://orcid.org/0000-0003-0739-026X"]},"institutions":[{"id":"https://openalex.org/I1331084196","display_name":"National Forensic Sciences University","ror":"https://ror.org/03fbv3w59","country_code":"IN","type":"education","lineage":["https://openalex.org/I1331084196"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Vishal Mevada","raw_affiliation_strings":["School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Forensic Sciences, National Forensic Sciences University, Gandhinagar, Gujarat","institution_ids":["https://openalex.org/I1331084196"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091839656","display_name":"Satish Kumar","orcid":"https://orcid.org/0000-0002-2993-3694","observed_orcids":["https://orcid.org/0000-0002-2993-3694"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Satish Kumar","raw_affiliation_strings":["National Forensic Sciences University, Bhopal Campus, Madhya Pradesh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Forensic Sciences University, Bhopal Campus, Madhya Pradesh","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088352332","display_name":"Neelkant Verma","orcid":"https://orcid.org/0000-0003-4274-1360","observed_orcids":["https://orcid.org/0000-0003-4274-1360"]},"institutions":[{"id":"https://openalex.org/I1331084196","display_name":"National Forensic Sciences University","ror":"https://ror.org/03fbv3w59","country_code":"IN","type":"education","lineage":["https://openalex.org/I1331084196"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Neelkant Verma","raw_affiliation_strings":["School of Medico-Legal Sciences, National Forensic Sciences University, Gandhinagar, Gujarat"],"raw_orcid":"https://orcid.org/0000-0003-4274-1360","affiliations":[{"raw_affiliation_string":"School of Medico-Legal Sciences, National Forensic Sciences University, Gandhinagar, Gujarat","institution_ids":["https://openalex.org/I1331084196"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088352332"],"corresponding_institution_ids":["https://openalex.org/I1331084196"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13325","display_name":"Dye analysis and toxicity","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11511","display_name":"Dyeing and Modifying Textile Fibers","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2507","display_name":"Polymers and Plastics"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.8769999742507935},{"id":"https://openalex.org/keywords/food-dyes","display_name":"food dyes","score":0.8289999961853027},{"id":"https://openalex.org/keywords/protein-ligand-interactions","display_name":"protein-ligand interactions","score":0.734000027179718},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.8339999914169312},{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.8930000066757202},{"id":"https://openalex.org/keywords/surface-hydrophobicity","display_name":"surface hydrophobicity","score":0.8360000252723694},{"id":"https://openalex.org/keywords/hemolysis","display_name":"hemolysis","score":0.871999979019165},{"id":"https://openalex.org/keywords/dye-toxicity","display_name":"dye toxicity","score":0.578000009059906},{"id":"https://openalex.org/keywords/hydrophobic-binding-site","display_name":"hydrophobic binding site","score":0.6299999952316284}],"concepts":[{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.7573999762535095},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7013999819755554},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5357000231742859},{"id":"https://openalex.org/C165616093","wikidata":"https://www.wikidata.org/wiki/Q424232","display_name":"Serum albumin","level":2,"score":0.41130000352859497},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.38119998574256897},{"id":"https://openalex.org/C2776125364","wikidata":"https://www.wikidata.org/wiki/Q169219","display_name":"Albumin","level":2,"score":0.3190000057220459},{"id":"https://openalex.org/C112705442","wikidata":"https://www.wikidata.org/wiki/Q323936","display_name":"Pharmacokinetics","level":2,"score":0.30090001225471497},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.2833000123500824},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.2815999984741211},{"id":"https://openalex.org/C136238340","wikidata":"https://www.wikidata.org/wiki/Q7251455","display_name":"Protein aggregation","level":2,"score":0.2766000032424927},{"id":"https://openalex.org/C3019542406","wikidata":"https://www.wikidata.org/wiki/Q21296145","display_name":"Food protein","level":2,"score":0.27459999918937683},{"id":"https://openalex.org/C2994376932","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Amino acid residue","level":4,"score":0.2653999924659729},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.26089999079704285},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.260699987411499}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.07.08.737154","doi":"https://doi.org/10.64898/2026.07.08.737154","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.08.737154","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/09/2026.07.08.737154.1.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.07.08.737154","doi":"https://doi.org/10.64898/2026.07.08.737154","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.07.08.737154","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/07/09/2026.07.08.737154.1.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334821","display_name":"Drug Applied Research Center, Tabriz University of Medical Sciences","ror":"https://ror.org/04krpx645"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7167840109.pdf","grobid_xml":"https://content.openalex.org/works/W7167840109.grobid-xml"},"referenced_works_count":40,"referenced_works":["https://openalex.org/W77085432","https://openalex.org/W1504792186","https://openalex.org/W1996080808","https://openalex.org/W2015349361","https://openalex.org/W2016452289","https://openalex.org/W2018607875","https://openalex.org/W2043256557","https://openalex.org/W2075582258","https://openalex.org/W2127642254","https://openalex.org/W2605874573","https://openalex.org/W2611332425","https://openalex.org/W2770929822","https://openalex.org/W2799767350","https://openalex.org/W2848548555","https://openalex.org/W2886456330","https://openalex.org/W2886976156","https://openalex.org/W2901426401","https://openalex.org/W2914944040","https://openalex.org/W2946901433","https://openalex.org/W3003280505","https://openalex.org/W3025508998","https://openalex.org/W3047157985","https://openalex.org/W3193325056","https://openalex.org/W3210301753","https://openalex.org/W3213313190","https://openalex.org/W4200368518","https://openalex.org/W4214701203","https://openalex.org/W4240415290","https://openalex.org/W4282927041","https://openalex.org/W4296696127","https://openalex.org/W4307338093","https://openalex.org/W4319159815","https://openalex.org/W4367596670","https://openalex.org/W4388018115","https://openalex.org/W4393861558","https://openalex.org/W4394619177","https://openalex.org/W4404063104","https://openalex.org/W4407357104","https://openalex.org/W4409266461","https://openalex.org/W4412366993"],"related_works":["https://openalex.org/W4393861558","https://openalex.org/W2043834112","https://openalex.org/W4392142135","https://openalex.org/W2064164421","https://openalex.org/W4407357104","https://openalex.org/W2803268551","https://openalex.org/W4292340364","https://openalex.org/W3007923829","https://openalex.org/W7165653213","https://openalex.org/W6902433001"],"abstract_inverted_index":{"Abstract":[0,221],"The":[1,34],"widespread":[2],"use":[3],"of":[4,114,129,135,166,173,177,212,216],"synthetic":[5],"food":[6,136],"dyes,":[7],"such":[8],"as":[9,87],"Acid":[10,142],"Yellow":[11,143],"23":[12,39,144,168,185,201],"(AY":[13,145],"23),":[14],"in":[15,57,186,208],"the":[16,67,110,126,133,170,178,187],"food,":[17],"cosmetics,":[18],"and":[19,31,40,52,84,103,153,194],"pharmaceutical":[20],"industries":[21],"raises":[22],"questions":[23],"about":[24],"their":[25],"potential":[26],"effects":[27,128],"on":[28],"biological":[29],"systems":[30],"public":[32,139],"health.":[33,140],"concentration-dependent":[35],"interaction":[36,190,202],"between":[37],"AY":[38,115,130,167,184,200,213],"bovine":[41],"serum":[42],"albumin":[43],"(BSA),":[44],"a":[45,90,122],"crucial":[46],"model":[47],"protein":[48,85],"for":[49,125,138],"understanding":[50],"pharmacokinetics":[51],"protein-ligand":[53],"behaviour,":[54],"was":[55,218],"examined":[56],"this":[58,119],"study.":[59],"We":[60],"demonstrate":[61],"that,":[62],"under":[63,157],"physiological":[64,158],"conditions,":[65],"increasing":[66],"dye":[68],"concentration":[69],"from":[70],"50":[71],"\u00b5M":[72,75],"to":[73,77,155,205],"200":[74],"leads":[76,154],"notable":[78],"conformational":[79],"changes,":[80],"increased":[81],"surface":[82],"hydrophobicity,":[83],"aggregation,":[86],"determined":[88],"using":[89],"multimodal":[91],"biophysical":[92],"approach":[93],"including":[94],"fluorescence":[95],"spectroscopy.":[96],"Direct":[97],"visualisation":[98],"verified":[99],"these":[100],"structural":[101],"changes":[102,181],"aggregate":[104],"formation,":[105],"whereas":[106],"hemolytic":[107,112,207],"assay":[108],"confirmed":[109],"high":[111],"nature":[113],"23-induced":[116],"fibrils.":[117],"Additionally,":[118],"study":[120],"provides":[121],"mechanistic":[123],"basis":[124],"toxicological":[127],"23,":[131],"underscoring":[132],"implications":[134],"dyes":[137],"Highlights":[141],"23)":[146],"modulates":[147],"Bovine":[148],"Serum":[149],"Albumin":[150],"(BSA)":[151],"structure":[152],"aggregation":[156],"conditions.":[159],"Structural":[160],"alteration":[161],"is":[162],"followed":[163],"by":[164,183],"binding":[165],"at":[169],"hydrophobic":[171],"regions":[172],"BSA,":[174],"causing":[175],"perturbation":[176,215],"protein.":[179],"Confirmational":[180],"induced":[182],"BSA":[188,217],"via":[189],"with":[191],"Asp108,":[192],"Pro110":[193],"Ala193.":[195],"Protein":[196],"intermediates":[197],"formed":[198],"after":[199],"are":[203],"observed":[204],"be":[206],"nature.":[209],"Molecular":[210],"mechanism":[211],"23-mediated":[214],"assessed.":[219],"Graphical":[220]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-07-10T00:00:00"}