{"id":"https://openalex.org/W7166345607","doi":"https://doi.org/10.18231/j.ijmr.14864.1778308462","title":"Validation of the loop-mediated isothermal amplification compared with real-time micro-PCR and conventional methods for the detection of pulmonary tuberculosis","display_name":"Validation of the loop-mediated isothermal amplification compared with real-time micro-PCR and conventional methods for the detection of pulmonary tuberculosis","publication_year":2026,"publication_date":"2026-06-27","ids":{"openalex":"https://openalex.org/W7166345607","doi":"https://doi.org/10.18231/j.ijmr.14864.1778308462"},"language":"en","primary_location":{"id":"doi:10.18231/j.ijmr.14864.1778308462","doi":"https://doi.org/10.18231/j.ijmr.14864.1778308462","is_oa":true,"landing_page_url":"https://doi.org/10.18231/j.ijmr.14864.1778308462","pdf_url":"https://ijmronline.org/archive/volume/13/issue/2/article/27171/pdf","source":{"id":"https://openalex.org/S2764356638","display_name":"Indian Journal of Microbiology Research","issn_l":"2394-546X","issn":["2394-546X","2394-5478"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian Journal of Microbiology Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://ijmronline.org/archive/volume/13/issue/2/article/27171/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002869243","display_name":"Kalpesh Jiva Khutade","orcid":"https://orcid.org/0000-0002-8293-4880","observed_orcids":["https://orcid.org/0000-0002-8293-4880"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kalpesh Jiva Khutade","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074815394","display_name":"Sandip K. Wagh","orcid":"https://orcid.org/0000-0002-5889-6220","observed_orcids":["https://orcid.org/0000-0002-5889-6220"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sandip K Wagh","raw_affiliation_strings":["Dept. of Biological Sciences , School of Sciences , Sandip University , Mahiravani , Maharashtra , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Biological Sciences , School of Sciences , Sandip University , Mahiravani , Maharashtra , India","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037967392","display_name":"Harshada Shah","orcid":"https://orcid.org/0009-0001-8963-7037","observed_orcids":["https://orcid.org/0009-0001-8963-7037"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Harshada Shah","raw_affiliation_strings":["Dept. of Microbiology , Vedantaa Institute of Medical Sciences (VIMS) , Palghar , Maharashtra , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Microbiology , Vedantaa Institute of Medical Sciences (VIMS) , Palghar , Maharashtra , India","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5074815394"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"13","issue":"2","first_page":"274","last_page":"280"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/loop-mediated-isothermal-amplification","display_name":"loop-mediated isothermal amplification","score":0.9449999928474426},{"id":"https://openalex.org/keywords/pulmonary-tuberculosis","display_name":"pulmonary tuberculosis","score":0.7739999890327454},{"id":"https://openalex.org/keywords/mycobacterium-tuberculosis-detection","display_name":"Mycobacterium tuberculosis detection","score":0.9860000014305115},{"id":"https://openalex.org/keywords/truenat","display_name":"Truenat","score":0.6779999732971191},{"id":"https://openalex.org/keywords/smear-microscopy","display_name":"smear microscopy","score":0.9010000228881836},{"id":"https://openalex.org/keywords/sputum-culture","display_name":"sputum culture","score":0.6779999732971191},{"id":"https://openalex.org/keywords/sensitivity-and-specificity","display_name":"sensitivity and specificity","score":0.8169999718666077},{"id":"https://openalex.org/keywords/rapid-molecular-diagnostics","display_name":"rapid molecular diagnostics","score":0.5659999847412109},{"id":"https://openalex.org/keywords/colorimetric-assay","display_name":"colorimetric assay","score":0.734000027179718}],"concepts":[{"id":"https://openalex.org/C60635243","wikidata":"https://www.wikidata.org/wiki/Q6675780","display_name":"Loop-mediated isothermal amplification","level":3,"score":0.7608000040054321},{"id":"https://openalex.org/C2776301714","wikidata":"https://www.wikidata.org/wiki/Q259346","display_name":"Sputum","level":3,"score":0.6521999835968018},{"id":"https://openalex.org/C2777975735","wikidata":"https://www.wikidata.org/wiki/Q130971","display_name":"Mycobacterium tuberculosis","level":3,"score":0.5985000133514404},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5796999931335449},{"id":"https://openalex.org/C2908628106","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Pulmonary tuberculosis","level":3,"score":0.5763000249862671},{"id":"https://openalex.org/C160798450","wikidata":"https://www.wikidata.org/wiki/Q4230870","display_name":"Concordance","level":2,"score":0.5687999725341797},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.5618000030517578},{"id":"https://openalex.org/C2986587452","wikidata":"https://www.wikidata.org/wiki/Q938438","display_name":"Statistical analysis","level":2,"score":0.3813000023365021},{"id":"https://openalex.org/C2779790121","wikidata":"https://www.wikidata.org/wiki/Q5779917","display_name":"Mycobacterium tuberculosis complex","level":4,"score":0.35740000009536743},{"id":"https://openalex.org/C3020132585","wikidata":"https://www.wikidata.org/wiki/Q2671652","display_name":"Diagnostic accuracy","level":2,"score":0.3052000105381012},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.2994999885559082},{"id":"https://openalex.org/C2780639635","wikidata":"https://www.wikidata.org/wiki/Q4888238","display_name":"Tuberculosis diagnosis","level":4,"score":0.28850001096725464},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.2831999957561493},{"id":"https://openalex.org/C2989005","wikidata":"https://www.wikidata.org/wiki/Q214963","display_name":"Nuclear medicine","level":1,"score":0.26579999923706055},{"id":"https://openalex.org/C42972112","wikidata":"https://www.wikidata.org/wiki/Q170201","display_name":"Veterinary medicine","level":1,"score":0.2578999996185303},{"id":"https://openalex.org/C40993552","wikidata":"https://www.wikidata.org/wiki/Q514654","display_name":"Gold standard (test)","level":2,"score":0.25699999928474426},{"id":"https://openalex.org/C3020221152","wikidata":"https://www.wikidata.org/wiki/Q18553330","display_name":"Extrapulmonary tuberculosis","level":4,"score":0.2515000104904175}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.18231/j.ijmr.14864.1778308462","doi":"https://doi.org/10.18231/j.ijmr.14864.1778308462","is_oa":true,"landing_page_url":"https://doi.org/10.18231/j.ijmr.14864.1778308462","pdf_url":"https://ijmronline.org/archive/volume/13/issue/2/article/27171/pdf","source":{"id":"https://openalex.org/S2764356638","display_name":"Indian Journal of Microbiology Research","issn_l":"2394-546X","issn":["2394-546X","2394-5478"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian Journal of Microbiology Research","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.18231/j.ijmr.14864.1778308462","doi":"https://doi.org/10.18231/j.ijmr.14864.1778308462","is_oa":true,"landing_page_url":"https://doi.org/10.18231/j.ijmr.14864.1778308462","pdf_url":"https://ijmronline.org/archive/volume/13/issue/2/article/27171/pdf","source":{"id":"https://openalex.org/S2764356638","display_name":"Indian Journal of Microbiology Research","issn_l":"2394-546X","issn":["2394-546X","2394-5478"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian Journal of Microbiology Research","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9537000060081482,"display_name":"Good health and well-being"}],"study_designs":[{"id":"https://openalex.org/study-designs/observational-study","display_name":"Observational Study"}],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7166345607.pdf","grobid_xml":"https://content.openalex.org/works/W7166345607.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W1505461031","https://openalex.org/W1655605933","https://openalex.org/W2025939591","https://openalex.org/W2105275554","https://openalex.org/W2153761254","https://openalex.org/W2165845866","https://openalex.org/W2573875784","https://openalex.org/W2726248205","https://openalex.org/W2754999686","https://openalex.org/W2964798605","https://openalex.org/W4200509774","https://openalex.org/W4286111042","https://openalex.org/W4313518773","https://openalex.org/W4362635948","https://openalex.org/W4390610371","https://openalex.org/W4392095841","https://openalex.org/W4394891174","https://openalex.org/W4404905612","https://openalex.org/W4405180761","https://openalex.org/W4406092311","https://openalex.org/W4412594401"],"related_works":["https://openalex.org/W2774710230","https://openalex.org/W2138240005","https://openalex.org/W2358146762","https://openalex.org/W2575925968","https://openalex.org/W3008900258","https://openalex.org/W2886498655","https://openalex.org/W3083074425","https://openalex.org/W3119133656","https://openalex.org/W2053464495","https://openalex.org/W7164668355"],"abstract_inverted_index":{"Background:":[0],"Efficient":[1],"diagnosis":[2],"and":[3,49,54,75,80,97,108,142,158,169,202],"proper":[4],"identification":[5],"of":[6,17,26,36,105,125,192],"Mycobacterium":[7],"tuberculosis":[8,18],"(MTB)":[9],"are":[10],"still":[11],"a":[12,37,121,199],"crucial":[13],"point":[14],"in":[15,21],"control":[16],"(TB),":[19],"especially":[20],"resource-poor":[22],"regions.":[23],"The":[24,180,194],"aim":[25],"this":[27],"study":[28],"was":[29,120,134,165,171,189],"to":[30,129,206],"determine":[31],"the":[32,57,87,132,146,177],"diagnostic":[33,123],"concordance":[34],"accuracy":[35,191],"colorimetric":[38,195],"Loop-Mediated":[39],"Isothermal":[40],"Amplification":[41],"(TB-LAMP)":[42],"assay":[43,48,197],"against":[44],"microscopy,":[45,78],"real-time":[46],"micro-PCR":[47],"Middlebrook":[50,90],"7H9":[51,91],"broth":[52,92],"culture.Materials":[53],"Methods:":[55],"In":[56],"World":[58],"Health":[59],"Organization":[60],"(WHO)":[61],"inclusion":[62],"criteria,":[63],"270":[64,111],"clinically":[65],"suspected":[66],"pulmonary":[67],"TB":[68],"patients'":[69],"sputum":[70],"samples":[71,88],"were":[72,84,100,115],"obtained.":[73],"Direct":[74],"concentrated":[76],"smear":[77,118],"TB-LAMP":[79,196],"TrueNat":[81,143,178],"for":[82,102,161,185,209],"MTB":[83,210],"performed":[85],"on":[86,145],"with":[89,176],"culture":[93,184],"(gold":[94],"standard).":[95],"McNemar\u2019s":[96],"Friedman":[98],"tests":[99],"used":[101],"statistical":[103],"analysis":[104],"sensitivity,":[106,152],"specificity":[107],"concordance.Results:":[109],"Among":[110],"samples,":[112],"112":[113],"(41.48%)":[114],"culture-positive.":[116],"Conventional":[117],"microscopy":[119],"modest":[122],"sensitivity":[124],"70.43%":[126],"that":[127],"rose":[128],"79.31%,":[130],"when":[131],"sample":[133],"concentrated.":[135],"Molecular":[136],"assays,":[137],"such":[138],"as":[139],"LAMP":[140],"(89.66%)":[141],"(93.97%),":[144],"other":[147],"hand,":[148],"presented":[149],"significantly":[150],"higher":[151],"demonstrating":[153],"their":[154],"improved":[155],"analytical":[156],"performance":[157],"decreased":[159],"risk":[160],"false-negative":[162],"results.":[163],"Specificity":[164],"also":[166],"substantially":[167],"enhanced,":[168],"it":[170],"98.7%":[172],"(95%":[173],"CI:":[174],"95.39-99.84)":[175],"assay.":[179],"reference":[181],"standard":[182],"test,":[183],"M.":[186],"tuberculosis,":[187],"unsurprisingly":[188],"100%":[190],"diagnosis.Conclusion:":[193],"provides":[198],"rapid,":[200],"sensitive,":[201],"highly":[203],"specific":[204],"alternative":[205],"conventional":[207],"diagnostics":[208],"detection.":[211]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-06-28T00:00:00"}