{"id":"https://openalex.org/W7166205175","doi":"https://doi.org/10.21178/2079-6080.2026.1.5","title":"Results of sequencing of aspen clones after editing of the LEAFY gene","display_name":"Results of sequencing of aspen clones after editing of the LEAFY gene","publication_year":2026,"publication_date":"2026-01-01","ids":{"openalex":"https://openalex.org/W7166205175","doi":"https://doi.org/10.21178/2079-6080.2026.1.5"},"language":"en","primary_location":{"id":"doi:10.21178/2079-6080.2026.1.5","doi":"https://doi.org/10.21178/2079-6080.2026.1.5","is_oa":true,"landing_page_url":"https://doi.org/10.21178/2079-6080.2026.1.5","pdf_url":"https://doi.org/10.21178/2079-6080.2026.1.5","source":{"id":"https://openalex.org/S4210211145","display_name":"Proceedings of the Saint Petersburg Forestry Research Institute","issn_l":"2079-6080","issn":["2079-6080"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the Saint Petersburg Forestry Research Institute","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21178/2079-6080.2026.1.5","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009375342","display_name":"Dmitry Karzhaev","orcid":"https://orcid.org/0000-0002-2444-9502","observed_orcids":["https://orcid.org/0000-0002-2444-9502"]},"institutions":[{"id":"https://openalex.org/I4210129604","display_name":"Saint Petersburg State Forest Technical University","ror":"https://ror.org/034882z59","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210129604"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"D.S. Karzhaev","raw_affiliation_strings":["( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S","institution_ids":["https://openalex.org/I4210129604"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5135413338","display_name":"\u041c. \u0412. \u0422\u0438\u0441","orcid":"https://orcid.org/0009-0009-6998-8823","observed_orcids":["https://orcid.org/0009-0009-6998-8823"]},"institutions":[{"id":"https://openalex.org/I4210129604","display_name":"Saint Petersburg State Forest Technical University","ror":"https://ror.org/034882z59","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210129604"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"M.V. Tis","raw_affiliation_strings":["( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S","institution_ids":["https://openalex.org/I4210129604"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035414832","display_name":"\u0414.\u0410. \u0428\u0430\u0431\u0443\u043d\u0438\u043d","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129604","display_name":"Saint Petersburg State Forest Technical University","ror":"https://ror.org/034882z59","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210129604"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"D.A. Shabunin","raw_affiliation_strings":["( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S","institution_ids":["https://openalex.org/I4210129604"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016492408","display_name":"O.Yu. Butenko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129604","display_name":"Saint Petersburg State Forest Technical University","ror":"https://ror.org/034882z59","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210129604"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"O.Yu. Butenko","raw_affiliation_strings":["( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S","institution_ids":["https://openalex.org/I4210129604"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091577020","display_name":"Elena Potokina","orcid":"https://orcid.org/0000-0002-2578-6279","observed_orcids":["https://orcid.org/0000-0002-2578-6279"]},"institutions":[{"id":"https://openalex.org/I4210129604","display_name":"Saint Petersburg State Forest Technical University","ror":"https://ror.org/034882z59","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210129604"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"E.K. Potokina","raw_affiliation_strings":["( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"( Saint-Petersburg Forestry Research Institute ; Saint-Petersburg State Forest Technical University named after S","institution_ids":["https://openalex.org/I4210129604"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210129604"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9312000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9312000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.04399999976158142,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspen","display_name":"aspen","score":0.8529999852180481},{"id":"https://openalex.org/keywords/poplar","display_name":"poplar","score":0.9190000295639038},{"id":"https://openalex.org/keywords/leafy-gene","display_name":"LEAFY gene","score":0.9660000205039978},{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.9539999961853027},{"id":"https://openalex.org/keywords/genetically-modified-trees","display_name":"genetically modified trees","score":0.8489999771118164},{"id":"https://openalex.org/keywords/inflorescence-formation","display_name":"inflorescence production","score":0.6729999780654907},{"id":"https://openalex.org/keywords/target-site-mutations","display_name":"target-site mutations","score":0.39399999380111694},{"id":"https://openalex.org/keywords/chimeric-plants","display_name":"chimeric plants","score":0.7419999837875366},{"id":"https://openalex.org/keywords/forest-tree-breeding","display_name":"forest tree breeding","score":0.46399998664855957}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7851999998092651},{"id":"https://openalex.org/C2781451354","wikidata":"https://www.wikidata.org/wiki/Q6508901","display_name":"Leafy","level":2,"score":0.5644000172615051},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5526000261306763},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5400999784469604},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.49570000171661377},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.48809999227523804},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.4302000105381012},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.4065000116825104},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.4025000035762787},{"id":"https://openalex.org/C106337198","wikidata":"https://www.wikidata.org/wiki/Q13564012","display_name":"Illumina dye sequencing","level":4,"score":0.39899998903274536},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.39890000224113464},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3986000120639801},{"id":"https://openalex.org/C51456166","wikidata":"https://www.wikidata.org/wiki/Q182726","display_name":"Genetically modified organism","level":3,"score":0.38359999656677246},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.36149999499320984},{"id":"https://openalex.org/C192953774","wikidata":"https://www.wikidata.org/wiki/Q7307127","display_name":"Reference genome","level":4,"score":0.35190001130104065},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.3483999967575073},{"id":"https://openalex.org/C44671064","wikidata":"https://www.wikidata.org/wiki/Q189800","display_name":"Gene pool","level":4,"score":0.3377000093460083},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.33629998564720154},{"id":"https://openalex.org/C2776978901","wikidata":"https://www.wikidata.org/wiki/Q5396348","display_name":"Tree breeding","level":3,"score":0.3330000042915344},{"id":"https://openalex.org/C169282265","wikidata":"https://www.wikidata.org/wiki/Q17145598","display_name":"Molecular breeding","level":3,"score":0.32919999957084656},{"id":"https://openalex.org/C81977670","wikidata":"https://www.wikidata.org/wiki/Q585259","display_name":"Genetic diversity","level":3,"score":0.3122999966144562},{"id":"https://openalex.org/C68873052","wikidata":"https://www.wikidata.org/wiki/Q349856","display_name":"Genetic variation","level":3,"score":0.31189998984336853},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.30169999599456787},{"id":"https://openalex.org/C18949551","wikidata":"https://www.wikidata.org/wiki/Q740578","display_name":"Sequence assembly","level":5,"score":0.2897000014781952},{"id":"https://openalex.org/C76818968","wikidata":"https://www.wikidata.org/wiki/Q181940","display_name":"Sanger sequencing","level":4,"score":0.28380000591278076},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.28029999136924744},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.26919999718666077}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.21178/2079-6080.2026.1.5","doi":"https://doi.org/10.21178/2079-6080.2026.1.5","is_oa":true,"landing_page_url":"https://doi.org/10.21178/2079-6080.2026.1.5","pdf_url":"https://doi.org/10.21178/2079-6080.2026.1.5","source":{"id":"https://openalex.org/S4210211145","display_name":"Proceedings of the Saint Petersburg Forestry Research Institute","issn_l":"2079-6080","issn":["2079-6080"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the Saint Petersburg Forestry Research Institute","raw_type":"journal-article"},{"id":"pmh:https://cyberleninka.ru/article/n/rezultaty-sekvenirovaniya-klonov-osiny-podvergshihsya-protsedure-redaktirovaniya-gena-leafy","doi":null,"is_oa":true,"landing_page_url":"https://cyberleninka.ru/article/n/rezultaty-sekvenirovaniya-klonov-osiny-podvergshihsya-protsedure-redaktirovaniya-gena-leafy","pdf_url":"https://cyberleninka.ru/article/n/rezultaty-sekvenirovaniya-klonov-osiny-podvergshihsya-protsedure-redaktirovaniya-gena-leafy/pdf","source":{"id":"https://openalex.org/S4306401404","display_name":"CyberLeninK (CyberLeninka)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"text"}],"best_oa_location":{"id":"doi:10.21178/2079-6080.2026.1.5","doi":"https://doi.org/10.21178/2079-6080.2026.1.5","is_oa":true,"landing_page_url":"https://doi.org/10.21178/2079-6080.2026.1.5","pdf_url":"https://doi.org/10.21178/2079-6080.2026.1.5","source":{"id":"https://openalex.org/S4210211145","display_name":"Proceedings of the Saint Petersburg Forestry Research Institute","issn_l":"2079-6080","issn":["2079-6080"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["russia-white-list-4"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the Saint Petersburg Forestry Research Institute","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7166205175.pdf","grobid_xml":"https://content.openalex.org/works/W7166205175.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W2166558811"],"related_works":["https://openalex.org/W7167157478","https://openalex.org/W4254328467","https://openalex.org/W4411895898","https://openalex.org/W4411896529","https://openalex.org/W4403606530","https://openalex.org/W4411895335","https://openalex.org/W4367155540","https://openalex.org/W4402052214","https://openalex.org/W4411896054","https://openalex.org/W4411895141"],"abstract_inverted_index":{"In":[0],"most":[1],"countries,":[2,22],"the":[3,41,47,66,91,99,154,175,185,191,202],"improvement":[4],"of":[5,51,70,77,90,101,108,136,164],"tree":[6],"species":[7],"traits":[8],"still":[9],"relies":[10],"on":[11,105],"traditional":[12],"classical":[13],"breeding":[14],"methods,":[15],"whereas":[16],"in":[17,40,44,58,60,98,201,206],"some":[18],"developed":[19],"or":[20],"leading":[21],"this":[23],"challenge":[24],"is":[25,130],"increasingly":[26],"addressed":[27],"by":[28],"employing":[29],"genetic":[30],"engineering":[31],"techniques.The":[32],"first":[33,56],"research":[34],"into":[35],"genetically":[36,52,109],"modifying":[37],"trees":[38,54,72],"began":[39],"United":[42],"States":[43],"1987,":[45],"while":[46],"large-scale":[48],"industrial":[49],"application":[50],"modified":[53,71,110],"was":[55,103,115],"introduced":[57],"China":[59],"2002.However,":[61],"there":[62],"are":[63,195],"concerns":[64],"regarding":[65],"potential":[67],"uncontrolled":[68],"spread":[69],"and":[73,151,161,216],"their":[74],"possible":[75],"displacement":[76],"natural":[78],"species.This":[79],"study":[80],"aimed":[81],"at":[82,174,184],"selecting":[83],"aspen":[84,111],"lines":[85],"with":[86,171,190],"an":[87],"edited":[88],"region":[89],"LEAFY":[92],"gene,":[93],"known":[94],"for":[95,117,132,147,213],"its":[96],"involvement":[97],"formation":[100],"inflorescences.Genotyping":[102],"performed":[104],"227":[106],"samples":[107,168,178],"clones.A":[112],"genomic":[113],"library":[114],"created":[116],"sequencing":[118,157],"using":[119],"250":[120],"bp":[121],"paired-end":[122],"MiSeq":[123],"(Illumina,":[124],"USA)":[125],"protocols.Softwear":[126],"were":[127,188],"used:":[128],"FastQC":[129],"used":[131],"a":[133,180],"quality":[134],"control":[135],"high":[137],"throughput":[138],"sequence":[139],"data,":[140,158],"Trimmomatic":[141],"-a":[142],"flexible":[143],"read":[144],"trimming":[145],"tool":[146],"Illumina":[148],"NGS":[149],"data":[150],"CRISPResso2":[152],"-for":[153],"analyzing":[155],"deep":[156],"enabling":[159],"rapid":[160],"intuitive":[162],"interpretation":[163],"genome":[165],"editing":[166],"experiments.Some":[167],"exhibited":[169],"alleles":[170],"induced":[172],"polymorphism":[173],"target":[176,186,203],"locus.Additionally,":[177],"containing":[179],"two-nucleotide":[181],"deletion":[182],"(GA/-)":[183],"site":[187],"identified.Samples":[189],"mutations":[192,200],"described":[193],"above":[194],"chimeric.Sequencing":[196],"revealed":[197],"that":[198],"CRISPR/Cas9-induced":[199],"gene":[204],"occurred":[205],"seven":[207],"plants.These":[208],"plants":[209],"serve":[210],"as":[211],"candidates":[212],"additional":[214],"studies":[215],"vegetative":[217],"reproduction":[218],"to":[219],"develop":[220],"experimental":[221],"forest":[222],"cultures.":[223]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2026-06-27T00:00:00"}