{"id":"https://openalex.org/W7165882011","doi":"https://doi.org/10.61326/actanatsci.v7i1.491","title":"LC-MS/MS-Based Phenolic Profiling of Flaxseed (Linum usitatissimum L.) and Safflower (Carthamus tinctorius L.) Seed Extracts and Their Antibacterial Activity Against Selected Fish Pathogenic Bacteria","display_name":"LC-MS/MS-Based Phenolic Profiling of Flaxseed (Linum usitatissimum L.) and Safflower (Carthamus tinctorius L.) Seed Extracts and Their Antibacterial Activity Against Selected Fish Pathogenic Bacteria","publication_year":2026,"publication_date":"2026-06-24","ids":{"openalex":"https://openalex.org/W7165882011","doi":"https://doi.org/10.61326/actanatsci.v7i1.491"},"language":"en","primary_location":{"id":"doi:10.61326/actanatsci.v7i1.491","doi":"https://doi.org/10.61326/actanatsci.v7i1.491","is_oa":true,"landing_page_url":"https://doi.org/10.61326/actanatsci.v7i1.491","pdf_url":"https://prensipjournals.com/ojs/index.php/actanatsci/article/download/491/323","source":{"id":"https://openalex.org/S4210198875","display_name":"Acta Natura et Scientia","issn_l":"2718-0638","issn":["2718-0638"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Natura et Scientia","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W7165882011"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://prensipjournals.com/ojs/index.php/actanatsci/article/download/491/323","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5130939066","display_name":"Mabrokah Adrees Rafallah Saed","orcid":"https://orcid.org/0000-0001-8983-2329","observed_orcids":["https://orcid.org/0000-0001-8983-2329","https://orcid.org/0009-0002-3023-1611"]},"institutions":[{"id":"https://openalex.org/I98905743","display_name":"Kastamonu University","ror":"https://ror.org/015scty35","country_code":"TR","type":"education","lineage":["https://openalex.org/I98905743"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Mabrokah Adrees Rafallah Saed","raw_affiliation_strings":["Kastamonu University, Postgraduate Education Institute, Department of Aquaculture, 37150, Kastamonu, T\u00fcrkiye"],"raw_orcid":"https://orcid.org/0000-0001-8983-2329","affiliations":[{"raw_affiliation_string":"Kastamonu University, Postgraduate Education Institute, Department of Aquaculture, 37150, Kastamonu, T\u00fcrkiye","institution_ids":["https://openalex.org/I98905743"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079735992","display_name":"Mustafa Karga","orcid":"https://orcid.org/0000-0003-0398-3236","observed_orcids":["https://orcid.org/0000-0003-0398-3236"]},"institutions":[{"id":"https://openalex.org/I98905743","display_name":"Kastamonu University","ror":"https://ror.org/015scty35","country_code":"TR","type":"education","lineage":["https://openalex.org/I98905743"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Mustafa Karga","raw_affiliation_strings":["Kastamonu University, Inebolu Vocational School, Department of Transportation Services, 37500, Kastamonu, T\u00fcrkiye"],"raw_orcid":"https://orcid.org/0000-0003-0398-3236","affiliations":[{"raw_affiliation_string":"Kastamonu University, Inebolu Vocational School, Department of Transportation Services, 37500, Kastamonu, T\u00fcrkiye","institution_ids":["https://openalex.org/I98905743"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017641495","display_name":"Osman Nezih Kenano\u011flu","orcid":"https://orcid.org/0000-0002-5097-6656","observed_orcids":["https://orcid.org/0000-0002-5097-6656"]},"institutions":[{"id":"https://openalex.org/I98905743","display_name":"Kastamonu University","ror":"https://ror.org/015scty35","country_code":"TR","type":"education","lineage":["https://openalex.org/I98905743"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Osman Nezih Kenano\u011flu","raw_affiliation_strings":["Kastamonu University, Faculty of Engineering and Architecture, Department of Food Engineering, 37150, Kastamonu, T\u00fcrkiye"],"raw_orcid":"https://orcid.org/0000-0002-5097-6656","affiliations":[{"raw_affiliation_string":"Kastamonu University, Faculty of Engineering and Architecture, Department of Food Engineering, 37150, Kastamonu, T\u00fcrkiye","institution_ids":["https://openalex.org/I98905743"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055854741","display_name":"Soner Bilen","orcid":"https://orcid.org/0000-0001-9459-8178","observed_orcids":["https://orcid.org/0000-0001-9459-8178"]},"institutions":[{"id":"https://openalex.org/I98905743","display_name":"Kastamonu University","ror":"https://ror.org/015scty35","country_code":"TR","type":"education","lineage":["https://openalex.org/I98905743"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Soner Bilen","raw_affiliation_strings":["Kastamonu University, Department of Veterinary Medicine, Ihsangazi Vocational School, 37250, Kastamonu, T\u00fcrkiye"],"raw_orcid":"https://orcid.org/0000-0001-9459-8178","affiliations":[{"raw_affiliation_string":"Kastamonu University, Department of Veterinary Medicine, Ihsangazi Vocational School, 37250, Kastamonu, T\u00fcrkiye","institution_ids":["https://openalex.org/I98905743"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I98905743"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"74","last_page":"84"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10225","display_name":"Essential Oils and Antimicrobial Activity","score":0.8300999999046326,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10225","display_name":"Essential Oils and Antimicrobial Activity","score":0.8300999999046326,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.14509999752044678,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12526","display_name":"Antimicrobial agents and applications","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/flaxseed","display_name":"flaxseed","score":0.878000020980835},{"id":"https://openalex.org/keywords/safflower","display_name":"safflower","score":1.0},{"id":"https://openalex.org/keywords/flax","display_name":"flax","score":0.9129999876022339},{"id":"https://openalex.org/keywords/phenolic-compounds","display_name":"phenolic compounds","score":0.8220000267028809},{"id":"https://openalex.org/keywords/seed-extract","display_name":"seed extract","score":0.703000009059906},{"id":"https://openalex.org/keywords/antibacterial-activity","display_name":"antibacterial activity","score":0.9890000224113464},{"id":"https://openalex.org/keywords/fish-pathogens","display_name":"fish pathogens","score":0.8029999732971191},{"id":"https://openalex.org/keywords/aeromonas-hydrophila","display_name":"Aeromonas hydrophila","score":0.9549999833106995},{"id":"https://openalex.org/keywords/aeromonas-salmonicida","display_name":"Aeromonas salmonicida","score":1.0},{"id":"https://openalex.org/keywords/yersinia-ruckeri","display_name":"Yersinia ruckeri","score":0.9729999899864197},{"id":"https://openalex.org/keywords/vibrio-anguillarum","display_name":"Vibrio anguillarum","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pseudomonas-putida","display_name":"Pseudomonas putida","score":0.9679999947547913},{"id":"https://openalex.org/keywords/tannic-acid","display_name":"tannic acid","score":0.8159999847412109},{"id":"https://openalex.org/keywords/trans-ferulic-acid","display_name":"trans-ferulic acid","score":0.9739999771118164},{"id":"https://openalex.org/keywords/minimum-inhibitory-concentration","display_name":"minimum inhibitory concentration","score":0.7179999947547913},{"id":"https://openalex.org/keywords/aquaculture-disease-management","display_name":"aquaculture disease control","score":0.8399999737739563},{"id":"https://openalex.org/keywords/natural-antimicrobials","display_name":"natural antimicrobials","score":0.6489999890327454},{"id":"https://openalex.org/keywords/methanol-extract","display_name":"methanol extract","score":0.4950000047683716}],"concepts":[{"id":"https://openalex.org/C2780104969","wikidata":"https://www.wikidata.org/wiki/Q4774530","display_name":"Antibacterial activity","level":3,"score":0.8062000274658203},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.5476999878883362},{"id":"https://openalex.org/C2778883795","wikidata":"https://www.wikidata.org/wiki/Q804517","display_name":"Pathogenic bacteria","level":3,"score":0.4973999857902527},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49219998717308044},{"id":"https://openalex.org/C176947019","wikidata":"https://www.wikidata.org/wiki/Q597889","display_name":"Minimum inhibitory concentration","level":3,"score":0.46889999508857727},{"id":"https://openalex.org/C2778362808","wikidata":"https://www.wikidata.org/wiki/Q375837","display_name":"Gallic acid","level":3,"score":0.4618000090122223},{"id":"https://openalex.org/C2779441561","wikidata":"https://www.wikidata.org/wiki/Q2697755","display_name":"Aeromonas hydrophila","level":3,"score":0.42910000681877136},{"id":"https://openalex.org/C2778224086","wikidata":"https://www.wikidata.org/wiki/Q414116","display_name":"Caffeic acid","level":3,"score":0.426800012588501},{"id":"https://openalex.org/C26381139","wikidata":"https://www.wikidata.org/wiki/Q1158816","display_name":"Minimum bactericidal concentration","level":4,"score":0.41920000314712524},{"id":"https://openalex.org/C2776795634","wikidata":"https://www.wikidata.org/wiki/Q161100","display_name":"Linum","level":2,"score":0.4032999873161316},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3917999863624573},{"id":"https://openalex.org/C556039675","wikidata":"https://www.wikidata.org/wiki/Q771035","display_name":"Traditional medicine","level":1,"score":0.3531999886035919},{"id":"https://openalex.org/C70899900","wikidata":"https://www.wikidata.org/wiki/Q290439","display_name":"Polyphenol","level":3,"score":0.3515999913215637},{"id":"https://openalex.org/C2781263971","wikidata":"https://www.wikidata.org/wiki/Q409478","display_name":"Quercetin","level":3,"score":0.334199994802475},{"id":"https://openalex.org/C3019249092","wikidata":"https://www.wikidata.org/wiki/Q274493","display_name":"Antibacterial agent","level":3,"score":0.30880001187324524},{"id":"https://openalex.org/C2778985185","wikidata":"https://www.wikidata.org/wiki/Q1756682","display_name":"Lactobacillus plantarum","level":4,"score":0.30309998989105225},{"id":"https://openalex.org/C2780697445","wikidata":"https://www.wikidata.org/wiki/Q181559","display_name":"Camphor","level":2,"score":0.30090001225471497},{"id":"https://openalex.org/C2778831176","wikidata":"https://www.wikidata.org/wiki/Q164785","display_name":"Cinnamic acid","level":2,"score":0.2987000048160553},{"id":"https://openalex.org/C2777810353","wikidata":"https://www.wikidata.org/wiki/Q393336","display_name":"Kaempferol","level":4,"score":0.28600001335144043},{"id":"https://openalex.org/C2781464872","wikidata":"https://www.wikidata.org/wiki/Q422044","display_name":"Ellagic acid","level":4,"score":0.2775000035762787},{"id":"https://openalex.org/C2780313371","wikidata":"https://www.wikidata.org/wiki/Q137647","display_name":"Aeromonas","level":3,"score":0.273499995470047},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.26919999718666077}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.61326/actanatsci.v7i1.491","doi":"https://doi.org/10.61326/actanatsci.v7i1.491","is_oa":true,"landing_page_url":"https://doi.org/10.61326/actanatsci.v7i1.491","pdf_url":"https://prensipjournals.com/ojs/index.php/actanatsci/article/download/491/323","source":{"id":"https://openalex.org/S4210198875","display_name":"Acta Natura et Scientia","issn_l":"2718-0638","issn":["2718-0638"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Natura et Scientia","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:72ef7888338d422985c930a7e806e5fa","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/72ef7888338d422985c930a7e806e5fa","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Acta Natura et Scientia, Vol 7, Iss 1 (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.61326/actanatsci.v7i1.491","doi":"https://doi.org/10.61326/actanatsci.v7i1.491","is_oa":true,"landing_page_url":"https://doi.org/10.61326/actanatsci.v7i1.491","pdf_url":"https://prensipjournals.com/ojs/index.php/actanatsci/article/download/491/323","source":{"id":"https://openalex.org/S4210198875","display_name":"Acta Natura et Scientia","issn_l":"2718-0638","issn":["2718-0638"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Natura et Scientia","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.6735000014305115},{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7165882011.pdf","grobid_xml":"https://content.openalex.org/works/W7165882011.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W309554214","https://openalex.org/W1963617975","https://openalex.org/W1983734647","https://openalex.org/W1986131294","https://openalex.org/W1993934912","https://openalex.org/W2031587322","https://openalex.org/W2046453700","https://openalex.org/W2100199827","https://openalex.org/W2104478003","https://openalex.org/W2153793431","https://openalex.org/W2193088100","https://openalex.org/W2783143882","https://openalex.org/W2903381708","https://openalex.org/W2940007259","https://openalex.org/W3083453980","https://openalex.org/W3138211511","https://openalex.org/W3185412706","https://openalex.org/W3208110055","https://openalex.org/W4213005140","https://openalex.org/W4322487933","https://openalex.org/W4386472514","https://openalex.org/W4393357343"],"related_works":["https://openalex.org/W4391064196","https://openalex.org/W3107718431","https://openalex.org/W2920566875","https://openalex.org/W7134886485","https://openalex.org/W2936308437","https://openalex.org/W7160833309","https://openalex.org/W7120648461","https://openalex.org/W2184201794","https://openalex.org/W1580932299","https://openalex.org/W2907112339"],"abstract_inverted_index":{"The":[0,53,67,82,178],"growing":[1],"demand":[2],"for":[3,74,80,184,244,288],"sustainable":[4],"disease-control":[5],"strategies":[6],"in":[7,12,30,301],"aquaculture":[8],"has":[9],"increased":[10],"interest":[11],"plant-derived":[13],"compounds":[14],"with":[15,118,193,214],"potential":[16],"antibacterial":[17,32,180,267,286],"activity":[18,33,158,181,210,238,268],"against":[19,48,166,187,211,228,241,269],"fish":[20,50,270],"pathogens.":[21,271],"This":[22],"study":[23],"investigated":[24],"the":[25,86,106,135,153,162,248,277],"LC-MS/MS-based":[26],"phenolic":[27,83,155],"profile":[28],"and":[29,41,61,76,96,130,150,175,190,198,233,259,300],"vitro":[31],"of":[34,85,196,218],"aqueous":[35,64,88,256],"methanolic":[36,257],"flaxseed":[37,75,107,185,258],"(Linum":[38],"usitatissimum":[39],"L.)":[40,45],"safflower":[42,136,260],"(Carthamus":[43],"tinctorius":[44],"seed":[46,137,204,261],"extracts":[47,54,279],"selected":[49],"pathogenic":[51],"bacteria.":[52],"were":[55,70,91,226],"obtained":[56],"using":[57],"40%":[58],"methanol,":[59],"concentrated,":[60],"prepared":[62],"as":[63,98,152,283],"stock":[65,68,89],"solutions.":[66],"concentrations":[69],"0.148":[71],"g":[72,78],"mL-1":[73,79],"0.101":[77],"safflower.":[81],"profiles":[84],"final":[87],"solutions":[90],"then":[92],"determined":[93],"by":[94,112,161],"LC-MS/MS":[95,102],"expressed":[97],"\u00b5g":[99,200,220],"L-1":[100],"(ppb).":[101],"analysis":[103],"showed":[104,206],"that":[105,255,276],"extract":[108,138,186,205,246],"was":[109,159,182,239],"mainly":[110],"characterized":[111],"a":[113],"tannic":[114],"acid-rich":[115],"profile,":[116],"together":[117],"trans-ferulic":[119,140],"acid,":[120,122,124,129,141,143,145,147,149],"caffeic":[121,148],"gallic":[123],"quercetin,":[125],"rutin":[126],"trihydrate,":[127],"cinnamic":[128,142],"2,5-dihydroxybenzoic":[131,144],"acid.":[132],"In":[133],"contrast,":[134],"contained":[139],"ellagic":[146],"quercetin":[151],"detected":[154,240],"constituents.":[156],"Antibacterial":[157],"tested":[160,249,278],"broth":[163],"microdilution":[164],"method":[165],"Aeromonas":[167,169],"hydrophila,":[168,230],"salmonicida,":[170,192,213],"Pseudomonas":[171],"putida,":[172],"Yersinia":[173],"ruckeri,":[174,232],"Vibrio":[176],"anguillarum.":[177,235],"strongest":[179],"observed":[183],"A.":[188,191,212,229],"hydrophila":[189],"MIC":[194,216,224],"values":[195,225],"25":[197],"50":[199,219],"mL-1,":[201,221],"respectively.":[202],"Safflower":[203],"its":[207],"highest":[208],"inhibitory":[209,237],"an":[215],"value":[217],"while":[222],"higher":[223],"recorded":[227],"Y.":[231],"V.":[234],"No":[236],"P.":[242],"putida":[243],"either":[245],"within":[247],"concentration":[250],"range.":[251],"These":[252],"results":[253],"indicate":[254],"extracts,":[262],"particularly":[263],"flaxseed,":[264],"have":[265],"species-dependent":[266],"Although":[272],"these":[273],"findings":[274],"suggest":[275],"may":[280],"be":[281],"considered":[282],"preliminary":[284],"natural":[285],"candidates":[287],"aquaculture-related":[289],"research,":[290],"further":[291],"studies":[292],"are":[293],"needed":[294],"to":[295],"evaluate":[296],"their":[297],"safety,":[298],"stability,":[299],"vivo":[302],"applicability.":[303]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2026-06-26T00:00:00"}