{"id":"https://openalex.org/W7154996923","doi":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","title":"Multiplexed immunofluorescence and RNA-FISH v1","display_name":"Multiplexed immunofluorescence and RNA-FISH v1","publication_year":2025,"publication_date":"2025-01-13","ids":{"openalex":"https://openalex.org/W7154996923","doi":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.yxmvm9zk9l3p/v1","doi":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","pdf_url":"https://www.protocols.io/view/multiplexed-immunofluorescence-and-rna-fish-dxmq7k5w.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/multiplexed-immunofluorescence-and-rna-fish-dxmq7k5w.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024492574","display_name":"Sarah van Veen","orcid":"https://orcid.org/0000-0002-7639-7847","observed_orcids":["https://orcid.org/0000-0002-7639-7847"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sarah van Veen","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-7639-7847","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023506109","display_name":"Justin T Savage","orcid":"https://orcid.org/0000-0002-1530-4942","observed_orcids":["https://orcid.org/0000-0002-1530-4942"]},"institutions":[{"id":"https://openalex.org/I4387154804","display_name":"Aligning Science Across Parkinson's","ror":"https://ror.org/03zj4c476","country_code":"US","type":"other","lineage":["https://openalex.org/I4387154804"]},{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE","US"],"is_corresponding":false,"raw_author_name":"Justin T Savage","raw_affiliation_strings":["KU Leuven ; Aligning Science Across Parkinson's \ue081ASAP\ue082 Collaborative Research Network , Chevy Chase , MD 20815 ;"],"raw_orcid":"https://orcid.org/0000-0002-1530-4942","affiliations":[{"raw_affiliation_string":"KU Leuven ; Aligning Science Across Parkinson's \ue081ASAP\ue082 Collaborative Research Network , Chevy Chase , MD 20815 ;","institution_ids":["https://openalex.org/I4387154804","https://openalex.org/I99464096"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037612007","display_name":"Dolores Irala","orcid":"https://orcid.org/0000-0001-5799-5342","observed_orcids":["https://orcid.org/0000-0001-5799-5342"]},"institutions":[{"id":"https://openalex.org/I4387154804","display_name":"Aligning Science Across Parkinson's","ror":"https://ror.org/03zj4c476","country_code":"US","type":"other","lineage":["https://openalex.org/I4387154804"]},{"id":"https://openalex.org/I99464096","display_name":"KU Leuven","ror":"https://ror.org/05f950310","country_code":"BE","type":"education","lineage":["https://openalex.org/I99464096"]}],"countries":["BE","US"],"is_corresponding":false,"raw_author_name":"Dolores Irala","raw_affiliation_strings":["KU Leuven ; Aligning Science Across Parkinson's \ue081ASAP\ue082 Collaborative Research Network , Chevy Chase , MD 20815 ;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"KU Leuven ; Aligning Science Across Parkinson's \ue081ASAP\ue082 Collaborative Research Network , Chevy Chase , MD 20815 ;","institution_ids":["https://openalex.org/I4387154804","https://openalex.org/I99464096"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4943999946117401,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4943999946117401,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.12300000339746475,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.09229999780654907,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/astrocytes","display_name":"astrocytes","score":0.9760000109672546},{"id":"https://openalex.org/keywords/visual-cortex","display_name":"visual cortex","score":0.9959999918937683},{"id":"https://openalex.org/keywords/postnatal-brain-development","display_name":"postnatal brain development","score":0.8899999856948853},{"id":"https://openalex.org/keywords/multiplex-immunofluorescence","display_name":"multiplex immunofluorescence","score":0.8349999785423279},{"id":"https://openalex.org/keywords/rna-fish","display_name":"RNA fluorescence in situ hybridization","score":0.9449999928474426},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.8338000178337097},{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.7224000096321106},{"id":"https://openalex.org/C2777542381","wikidata":"https://www.wikidata.org/wiki/Q502961","display_name":"Astrocyte","level":3,"score":0.5616999864578247},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.5591999888420105},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4912000000476837},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4537000060081482},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.420199990272522},{"id":"https://openalex.org/C2777348757","wikidata":"https://www.wikidata.org/wiki/Q2346577","display_name":"Cortex (anatomy)","level":2,"score":0.41769999265670776},{"id":"https://openalex.org/C19275194","wikidata":"https://www.wikidata.org/wiki/Q222903","display_name":"Multiplexing","level":2,"score":0.4101000130176544},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39959999918937683},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.39640000462532043},{"id":"https://openalex.org/C2779345533","wikidata":"https://www.wikidata.org/wiki/Q75785","display_name":"Visual cortex","level":2,"score":0.382099986076355},{"id":"https://openalex.org/C2984894117","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Indirect immunofluorescence","level":3,"score":0.3619000017642975},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3345000147819519},{"id":"https://openalex.org/C3017456870","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Specific antibody","level":3,"score":0.334199994802475},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.31369999051094055},{"id":"https://openalex.org/C2781041448","wikidata":"https://www.wikidata.org/wiki/Q75839","display_name":"Cerebral cortex","level":2,"score":0.3005000054836273},{"id":"https://openalex.org/C35866371","wikidata":"https://www.wikidata.org/wiki/Q6005502","display_name":"Immunocytochemistry","level":2,"score":0.2831999957561493},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.2741999924182892},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.27059999108314514},{"id":"https://openalex.org/C52909226","wikidata":"https://www.wikidata.org/wiki/Q5462835","display_name":"Fluorescent labelling","level":3,"score":0.2632000148296356},{"id":"https://openalex.org/C21269302","wikidata":"https://www.wikidata.org/wiki/Q7243098","display_name":"Primary and secondary antibodies","level":3,"score":0.2578999996185303},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.25130000710487366}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.yxmvm9zk9l3p/v1","doi":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","pdf_url":"https://www.protocols.io/view/multiplexed-immunofluorescence-and-rna-fish-dxmq7k5w.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.yxmvm9zk9l3p/v1","doi":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.yxmvm9zk9l3p/v1","pdf_url":"https://www.protocols.io/view/multiplexed-immunofluorescence-and-rna-fish-dxmq7k5w.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7154996923.pdf","grobid_xml":"https://content.openalex.org/works/W7154996923.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4220845334","https://openalex.org/W4407684356","https://openalex.org/W2072812579","https://openalex.org/W3007259126","https://openalex.org/W3176072693","https://openalex.org/W2621128683","https://openalex.org/W4308973896","https://openalex.org/W3166762809","https://openalex.org/W7119510047","https://openalex.org/W4253434888"],"abstract_inverted_index":{"This":[0,78],"protocol":[1],"describes":[2],"a":[3,74],"multiplexed":[4],"immunofluorescence":[5],"and":[6,27,39,54,64],"RNA-FISH":[7],"method":[8],"to":[9,36,51],"detect":[10],"Atp13a4":[11,43,65,85],"mRNA":[12],"in":[13],"astrocytes":[14,38],"of":[15,84],"the":[16,52],"visual":[17],"cortex":[18],"at":[19],"postnatal":[20,88],"stages.":[21],"Brain":[22],"sections":[23],"from":[24],"P7,":[25],"P14,":[26],"P21":[28],"Aldh1L1-EGFP":[29],"mice":[30],"are":[31,58,67],"immunostained":[32],"with":[33,41],"anti-GFP":[34],"antibodies":[35],"label":[37],"hybridized":[40],"custom":[42,76],"probes.":[44],"Amplification":[45],"is":[46],"performed":[47],"using":[48,60,73],"hairpins":[49],"specific":[50],"probes":[53],"initiator-labeled":[55],"antibodies.":[56],"Sections":[57],"imaged":[59],"high-resolution":[61],"confocal":[62],"microscopy,":[63],"puncta":[66],"quantified":[68],"within":[69],"GFP-positive":[70],"astrocyte":[71],"regions":[72],"FIJI-based":[75],"pipeline.":[77],"approach":[79],"enables":[80],"spatially":[81],"resolved":[82],"analysis":[83],"expression":[86],"during":[87],"development.":[89]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-04-21T00:00:00"}
