{"id":"https://openalex.org/W7141273162","doi":"https://doi.org/10.64898/2026.03.25.713648","title":"Mining functional genes and characterizing cellular transcriptomic profiles in the single-cell atlas of adult Spodoptera litura ovary","display_name":"Mining functional genes and characterizing cellular transcriptomic profiles in the single-cell atlas of adult Spodoptera litura ovary","publication_year":2026,"publication_date":"2026-03-27","ids":{"openalex":"https://openalex.org/W7141273162","doi":"https://doi.org/10.64898/2026.03.25.713648"},"language":"en","primary_location":{"id":"doi:10.64898/2026.03.25.713648","doi":"https://doi.org/10.64898/2026.03.25.713648","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.25.713648","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.03.25.713648","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100562783","display_name":"Zhipeng Sun","orcid":"https://orcid.org/0009-0006-5315-4822","observed_orcids":["https://orcid.org/0009-0006-5315-4822"]},"institutions":[{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhipeng Sun","raw_affiliation_strings":["Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","institution_ids":["https://openalex.org/I4210151987"]},{"raw_affiliation_string":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","institution_ids":[]},{"raw_affiliation_string":"National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130811134","display_name":"Liwei Jiang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Liwei Jiang","raw_affiliation_strings":["Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","institution_ids":["https://openalex.org/I4210151987"]},{"raw_affiliation_string":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","institution_ids":[]},{"raw_affiliation_string":"National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130769091","display_name":"Xue Dong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xue Dong","raw_affiliation_strings":["Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","institution_ids":["https://openalex.org/I4210151987"]},{"raw_affiliation_string":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","institution_ids":[]},{"raw_affiliation_string":"National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054702011","display_name":"Xin Yi","orcid":"https://orcid.org/0000-0002-1639-6324","observed_orcids":["https://orcid.org/0000-0002-1639-6324"]},"institutions":[{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xin Yi","raw_affiliation_strings":["Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China"],"raw_orcid":"https://orcid.org/0000-0002-1639-6324","affiliations":[{"raw_affiliation_string":"Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","institution_ids":["https://openalex.org/I4210151987"]},{"raw_affiliation_string":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","institution_ids":[]},{"raw_affiliation_string":"National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037308478","display_name":"Todd G. Nystul","orcid":"https://orcid.org/0000-0002-6250-2394","observed_orcids":["https://orcid.org/0000-0002-6250-2394"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Todd G Nystul","raw_affiliation_strings":["Department of Anatomy, School of Medicine, University of California, San Francisco, CA, USA"],"raw_orcid":"https://orcid.org/0000-0002-6250-2394","affiliations":[{"raw_affiliation_string":"Department of Anatomy, School of Medicine, University of California, San Francisco, CA, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5130721716","display_name":"Guohua Zhong","orcid":"https://orcid.org/0000-0003-0207-4890","observed_orcids":["https://orcid.org/0000-0003-0207-4890"]},"institutions":[{"id":"https://openalex.org/I4210151987","display_name":"Ministry of Agriculture and Rural Affairs","ror":"https://ror.org/05ckt8b96","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210151987"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Guohua Zhong","raw_affiliation_strings":["Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China"],"raw_orcid":"https://orcid.org/0000-0003-0207-4890","affiliations":[{"raw_affiliation_string":"Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture and Rural Affairs, P. R. China","institution_ids":["https://openalex.org/I4210151987"]},{"raw_affiliation_string":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, P. R. China","institution_ids":[]},{"raw_affiliation_string":"National Key Laboratory of Green Pesticide, P. R. China; Guangzhou 510642, China","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5130721716"],"corresponding_institution_ids":["https://openalex.org/I4210151987"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spodoptera-litura","display_name":"Spodoptera litura","score":1.0},{"id":"https://openalex.org/keywords/insect-ovary","display_name":"insect ovary","score":0.7929999828338623},{"id":"https://openalex.org/keywords/oogenesis","display_name":"oogenesis","score":0.8450000286102295},{"id":"https://openalex.org/keywords/single-cell-transcriptomics","display_name":"single-cell transcriptomics","score":0.7919999957084656},{"id":"https://openalex.org/keywords/germ-cells","display_name":"germ cells","score":0.6539999842643738},{"id":"https://openalex.org/keywords/somatic-cells","display_name":"somatic cells","score":0.8489999771118164},{"id":"https://openalex.org/keywords/polo","display_name":"polo","score":0.8809999823570251},{"id":"https://openalex.org/keywords/paths","display_name":"paths","score":0.7549999952316284},{"id":"https://openalex.org/keywords/fecundity","display_name":"fecundity","score":0.9639999866485596},{"id":"https://openalex.org/keywords/intercellular-communication","display_name":"intercellular communication","score":0.8339999914169312},{"id":"https://openalex.org/keywords/rnai-based-pest-control","display_name":"RNAi-based pest control","score":0.656000018119812},{"id":"https://openalex.org/keywords/drosophila-melanogaster-comparison","display_name":"Drosophila melanogaster comparison","score":0.8659999966621399}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9160000085830688},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.728600025177002},{"id":"https://openalex.org/C2776273252","wikidata":"https://www.wikidata.org/wiki/Q27294","display_name":"Spodoptera litura","level":3,"score":0.5673999786376953},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5447999835014343},{"id":"https://openalex.org/C2780104201","wikidata":"https://www.wikidata.org/wiki/Q130888","display_name":"Drosophila melanogaster","level":3,"score":0.535099983215332},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5350000262260437},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5109000205993652},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.46639999747276306},{"id":"https://openalex.org/C109825262","wikidata":"https://www.wikidata.org/wiki/Q1139164","display_name":"Germline","level":3,"score":0.44600000977516174},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4440000057220459},{"id":"https://openalex.org/C163949779","wikidata":"https://www.wikidata.org/wiki/Q507032","display_name":"Oogenesis","level":4,"score":0.43799999356269836},{"id":"https://openalex.org/C161078062","wikidata":"https://www.wikidata.org/wiki/Q1068690","display_name":"Functional genomics","level":5,"score":0.4311999976634979},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.39100000262260437},{"id":"https://openalex.org/C107397762","wikidata":"https://www.wikidata.org/wiki/Q2542347","display_name":"RNA-Seq","level":5,"score":0.38199999928474426},{"id":"https://openalex.org/C18431079","wikidata":"https://www.wikidata.org/wiki/Q1502169","display_name":"Gene expression profiling","level":4,"score":0.36890000104904175},{"id":"https://openalex.org/C134305767","wikidata":"https://www.wikidata.org/wiki/Q725508","display_name":"Somatic cell","level":3,"score":0.3659000098705292},{"id":"https://openalex.org/C2778324911","wikidata":"https://www.wikidata.org/wiki/Q9631","display_name":"Ovary","level":2,"score":0.3424000144004822},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.3370000123977661},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.3167000114917755},{"id":"https://openalex.org/C22508944","wikidata":"https://www.wikidata.org/wiki/Q568174","display_name":"PEST analysis","level":2,"score":0.3131999969482422},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.31279999017715454},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.30820000171661377},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.27239999175071716},{"id":"https://openalex.org/C2778060650","wikidata":"https://www.wikidata.org/wiki/Q22964789","display_name":"Gene drive","level":4,"score":0.25690001249313354},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.2500999867916107}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.03.25.713648","doi":"https://doi.org/10.64898/2026.03.25.713648","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.25.713648","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.03.25.713648","doi":"https://doi.org/10.64898/2026.03.25.713648","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.25.713648","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.40450000762939453,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3643404537","display_name":null,"funder_award_id":"32500403)","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W2024836818","https://openalex.org/W2754894468","https://openalex.org/W2759996086","https://openalex.org/W2886171491","https://openalex.org/W2947129737","https://openalex.org/W3019976500","https://openalex.org/W3096898958","https://openalex.org/W3198791465","https://openalex.org/W4200473482","https://openalex.org/W4289856907","https://openalex.org/W4306248093","https://openalex.org/W4318170663","https://openalex.org/W4362585970","https://openalex.org/W4376617254","https://openalex.org/W4380686337","https://openalex.org/W4385074293","https://openalex.org/W4386020125","https://openalex.org/W4386413510","https://openalex.org/W4390543721","https://openalex.org/W4392591428","https://openalex.org/W4392705856","https://openalex.org/W4400880524","https://openalex.org/W4402196816","https://openalex.org/W4405598300","https://openalex.org/W4405671317","https://openalex.org/W4406421179","https://openalex.org/W4407052186"],"related_works":["https://openalex.org/W3019976500","https://openalex.org/W6920662047","https://openalex.org/W2979405122","https://openalex.org/W4402398745","https://openalex.org/W4384664950","https://openalex.org/W2754593097","https://openalex.org/W4391729331","https://openalex.org/W7221469547","https://openalex.org/W6901959672","https://openalex.org/W2969434730"],"abstract_inverted_index":{"Abstract":[0],"Understanding":[1],"the":[2,12,28,83,115],"reproductive":[3,173],"biology":[4,174],"of":[5,15,27,31,77,87,117,141],"non-model":[6,176],"organisms":[7],"remains":[8],"challenging":[9],"due":[10],"to":[11,56,98,113,124],"limited":[13],"availability":[14],"high-resolution":[16],"molecular":[17,101],"resources.":[18],"Here,":[19],"we":[20,63,90],"present":[21],"a":[22,38,44,138,160,169],"comprehensive":[23],"single-cell":[24,50],"transcriptomic":[25,92],"atlas":[26],"adult":[29],"ovary":[30],"Spodoptera":[32],"litura":[33,36,154],"(":[34,59],"S.":[35,153],"),":[37,62],"highly":[39],"polyphagous":[40],"agricultural":[41],"pest":[42,184],"with":[43,53,94,132],"polytrophic":[45],"meroistuc":[46],"ovary.":[47],"By":[48],"integrating":[49],"RNA":[51,110],"sequencing":[52],"cross-species":[54],"comparison":[55],"Drosophila":[57],"melanogaster":[58,61],"D.":[60],"define":[64],"major":[65],"germline":[66],"and":[67,71,74,85,127,134,145,178],"somatic":[68],"cell":[69,79,105],"populations":[70],"delineate":[72],"conserved":[73],"species-specific":[75],"features":[76],"ovarian":[78,104,125],"composition.":[80],"To":[81],"enhance":[82],"interpretability":[84],"reuse":[86],"this":[88,157],"dataset,":[89],"combine":[91],"profiling":[93],"in":[95,152,175],"situ":[96],"hybridization":[97],"validate":[99],"cluster-specific":[100],"markers":[102],"across":[103],"types.":[106],"We":[107],"further":[108],"apply":[109],"interference":[111],"(RNAi)":[112],"assess":[114],"contributions":[116],"germline-enriched":[118],"genes":[119],"(Hsc70-4,":[120],"Wech,":[121],"Polo,":[122],"Path)":[123],"development":[126],"fecundity.":[128],"Trajectory":[129],"inference,":[130],"together":[131],"SCENIC":[133],"CellChat":[135],"analyses,":[136],"provides":[137],"system-level":[139],"view":[140],"transcriptional":[142],"regulatory":[143],"programs":[144],"predicted":[146],"intercellular":[147],"communication":[148],"pathways":[149],"during":[150],"oogenesis":[151],".":[155],"Collectively,":[156],"work":[158],"establishes":[159],"valuable":[161],"resource":[162],"for":[163,172,182],"studying":[164],"lepidopteran":[165],"insect":[166],"oogenesis,":[167],"offering":[168],"comparative":[170],"framework":[171],"insects":[177],"highlighting":[179],"potential":[180],"targets":[181],"RNAi-based":[183],"control":[185],"strategies.":[186]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-03-28T00:00:00"}