{"id":"https://openalex.org/W7140328226","doi":"https://doi.org/10.7554/elife.110906.1","title":"Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability","display_name":"Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability","publication_year":2026,"publication_date":"2026-03-25","ids":{"openalex":"https://openalex.org/W7140328226","doi":"https://doi.org/10.7554/elife.110906.1"},"language":"en","primary_location":{"id":"doi:10.7554/elife.110906.1","doi":"https://doi.org/10.7554/elife.110906.1","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.110906.1","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.110906.1","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5130547840","display_name":"Youcai Xiong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Youcai Xiong","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077845913","display_name":"Li-Kuang Tsai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Li-Kuang Tsai","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006110795","display_name":"Jun Zhou","orcid":"https://orcid.org/0000-0002-2576-7692","observed_orcids":["https://orcid.org/0000-0002-2576-7692"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jun Zhou","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130606717","display_name":"Shuang Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shuang Chen","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130553245","display_name":"Xiaofeng Xia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiaofeng Xia","raw_affiliation_strings":["Research & Development, ATGC Inc"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research & Development, ATGC Inc","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058763957","display_name":"Jifeng Zhang","orcid":"https://orcid.org/0000-0001-5161-4705","observed_orcids":["https://orcid.org/0000-0001-5161-4705"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jifeng Zhang","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130555532","display_name":"Y Eugene Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y Eugene Chen","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130586494","display_name":"Jie Xu","orcid":"https://orcid.org/0000-0001-9452-2878","observed_orcids":["https://orcid.org/0000-0001-9452-2878"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jie Xu","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072178740","display_name":"Xiaoqiang Huang","orcid":"https://orcid.org/0000-0002-1005-848X","observed_orcids":["https://orcid.org/0000-0002-1005-848X"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaoqiang Huang","raw_affiliation_strings":["Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School"],"raw_orcid":"https://orcid.org/0000-0002-1005-848X","affiliations":[{"raw_affiliation_string":"Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sacas9","display_name":"SaCas9","score":0.9580000042915344},{"id":"https://openalex.org/keywords/protospacer-adjacent-motif","display_name":"protospacer adjacent motif","score":0.8309999704360962},{"id":"https://openalex.org/keywords/computational-protein-design","display_name":"computational protein design","score":0.6420000195503235},{"id":"https://openalex.org/keywords/genome-editing-efficiency","display_name":"genome editing efficiency","score":0.6209999918937683},{"id":"https://openalex.org/keywords/base-editing","display_name":"base editing","score":0.9570000171661377},{"id":"https://openalex.org/keywords/in-vivo-genome-editing","display_name":"in vivo gene editing","score":0.6150000095367432}],"concepts":[{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7642999887466431},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.6553999781608582},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6477000117301941},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.569100022315979},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5202000141143799},{"id":"https://openalex.org/C53105671","wikidata":"https://www.wikidata.org/wiki/Q14777816","display_name":"Rational design","level":2,"score":0.48559999465942383},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.47429999709129333},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43630000948905945},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.4336000084877014},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.4205000102519989},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.40860000252723694},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3939000070095062},{"id":"https://openalex.org/C66024118","wikidata":"https://www.wikidata.org/wiki/Q1122506","display_name":"Computational model","level":2,"score":0.3774000108242035},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3767000138759613},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.3610999882221222},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.3312000036239624},{"id":"https://openalex.org/C35802184","wikidata":"https://www.wikidata.org/wiki/Q204773","display_name":"Zinc finger nuclease","level":5,"score":0.32519999146461487},{"id":"https://openalex.org/C179799912","wikidata":"https://www.wikidata.org/wiki/Q205084","display_name":"Computational complexity theory","level":2,"score":0.299699991941452},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.29280000925064087},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.2854999899864197},{"id":"https://openalex.org/C66746571","wikidata":"https://www.wikidata.org/wiki/Q1134833","display_name":"ENCODE","level":3,"score":0.27869999408721924},{"id":"https://openalex.org/C125411270","wikidata":"https://www.wikidata.org/wiki/Q18653","display_name":"Encoding (memory)","level":2,"score":0.2709999978542328},{"id":"https://openalex.org/C152769699","wikidata":"https://www.wikidata.org/wiki/Q410814","display_name":"Protein design","level":3,"score":0.26739999651908875},{"id":"https://openalex.org/C14430832","wikidata":"https://www.wikidata.org/wiki/Q412550","display_name":"Integrase","level":3,"score":0.25369998812675476}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.7554/elife.110906.1","doi":"https://doi.org/10.7554/elife.110906.1","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.110906.1","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.7554/elife.110906.1","doi":"https://doi.org/10.7554/elife.110906.1","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.110906.1","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W1922040927","https://openalex.org/W1988101725","https://openalex.org/W2050480410","https://openalex.org/W2070614858","https://openalex.org/W2096261947","https://openalex.org/W2107959600","https://openalex.org/W2115603949","https://openalex.org/W2142515819","https://openalex.org/W2158637234","https://openalex.org/W2557181747","https://openalex.org/W2914667215","https://openalex.org/W2915459742","https://openalex.org/W2926667013","https://openalex.org/W2979549406","https://openalex.org/W3012327875","https://openalex.org/W3123122710","https://openalex.org/W4285395443","https://openalex.org/W4366464000","https://openalex.org/W4384023657","https://openalex.org/W4396721167","https://openalex.org/W4409730456","https://openalex.org/W7117353875","https://openalex.org/W7134109315"],"related_works":["https://openalex.org/W7155224276","https://openalex.org/W1922040927","https://openalex.org/W7154623531","https://openalex.org/W7170085633","https://openalex.org/W2926667013","https://openalex.org/W7124170637","https://openalex.org/W2561834051","https://openalex.org/W4245053291","https://openalex.org/W7212025479","https://openalex.org/W7212076588"],"abstract_inverted_index":{"CRISPR\u2013Cas9":[0],"nucleases":[1,221],"have":[2],"transformed":[3],"genome":[4,232],"engineering,":[5],"yet":[6],"their":[7],"application":[8],"is":[9,21],"often":[10],"constrained":[11],"by":[12,119,146],"protospacer-adjacent":[13],"motif":[14],"(PAM)":[15],"requirements.":[16],"Staphylococcus":[17],"aureus":[18],"Cas9":[19,207],"(SaCas9)":[20],"particularly":[22],"attractive":[23],"for":[24,125,205,217],"in":[25,130,193],"vivo":[26],"applications":[27],"due":[28],"to":[29,89,180,229],"its":[30,34,164],"compact":[31],"size;":[32],"however,":[33],"NNGRRT":[35],"PAM":[36,74,83,127,144,154,183],"limits":[37],"targetable":[38],"genomic":[39],"sites.":[40],"Here,":[41],"we":[42],"report":[43],"KRH,":[44],"a":[45,55,122,174,202,215],"SaCas9":[46],"variant":[47],"designed":[48],"entirely":[49],"from":[50],"the":[51,92,112,126,148,160,182,224],"wild-type":[52],"enzyme":[53],"through":[54],"fully":[56],"computational":[57,170,227],"point-mutation":[58],"design":[59,171,228],"workflow,":[60],"UniDesign,":[61],"without":[62],"additional":[63],"experimental":[64],"optimization.":[65],"As":[66],"expected,":[67],"KRH":[68,100,132,142,167,213],"efficiently":[69],"recognizes":[70],"an":[71],"expanded":[72],"NNNRRT":[73],"and":[75,103,133,137,156,222],"exhibits":[76],"substantially":[77],"enhanced":[78],"editing":[79],"efficiency":[80],"at":[81],"non-canonical":[82],"sites,":[84],"with":[85,135,153,159],"improvements":[86],"of":[87,111,177,198,226],"up":[88],"116-fold":[90],"over":[91],"wild":[93],"type.":[94],"Across":[95],"multiple":[96],"human":[97],"cell":[98],"types,":[99],"achieves":[101],"genome-":[102],"base-editing":[104],"efficiencies":[105],"comparable":[106],"to,":[107],"or":[108],"exceeding,":[109],"those":[110],"well-known":[113],"evolution-derived":[114,199],"KKH":[115],"variant.":[116],"Computational":[117],"modeling":[118],"UniDesign":[120],"provides":[121],"mechanistic":[123],"explanation":[124],"relaxation":[128],"observed":[129],"both":[131],"KKH,":[134],"structural":[136],"energetic":[138],"analyses":[139],"revealing":[140],"that":[141,169],"relaxes":[143],"specificity":[145],"fine-tuning":[147],"balance":[149],"between":[150],"sequence-specific":[151],"interactions":[152],"bases":[155],"nonspecific":[157],"contacts":[158],"DNA":[161],"backbone.":[162],"Beyond":[163],"practical":[165],"utility,":[166],"demonstrates":[168],"can":[172],"identify":[173],"minimal":[175],"set":[176],"mutations":[178],"sufficient":[179],"remodel":[181],"interface":[184],"while":[185],"preserving":[186],"high":[187],"nuclease":[188],"activity.":[189],"This":[190],"approach":[191],"recapitulates\u2014and":[192],"some":[194],"cases":[195],"surpasses\u2014the":[196],"performance":[197],"variants,":[200],"offering":[201],"scalable":[203],"strategy":[204],"high-throughput":[206],"engineering.":[208],"Overall,":[209],"these":[210],"results":[211],"establish":[212],"as":[214],"blueprint":[216],"rationally":[218],"engineered,":[219],"PAM-relaxed":[220],"underscores":[223],"power":[225],"accelerate":[230],"next-generation":[231],"editing.":[233]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-03-26T00:00:00"}
