{"id":"https://openalex.org/W7140102899","doi":"https://doi.org/10.64898/2026.03.19.709872","title":"In\u2011Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing","display_name":"In\u2011Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing","publication_year":2026,"publication_date":"2026-03-23","ids":{"openalex":"https://openalex.org/W7140102899","doi":"https://doi.org/10.64898/2026.03.19.709872","pmid":"https://pubmed.ncbi.nlm.nih.gov/41929115","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13041984"},"language":"en","primary_location":{"id":"doi:10.64898/2026.03.19.709872","doi":"https://doi.org/10.64898/2026.03.19.709872","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.19.709872","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/23/2026.03.19.709872.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/23/2026.03.19.709872.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5130332422","display_name":"Enrique O. Ruiz","orcid":"https://orcid.org/0009-0001-5276-2846","observed_orcids":["https://orcid.org/0009-0001-5276-2846"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Enrique O. Ruiz","raw_affiliation_strings":["Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA"],"raw_orcid":"https://orcid.org/0009-0001-5276-2846","affiliations":[{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA","institution_ids":["https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5092076762","display_name":"Kayla Neyra","orcid":"https://orcid.org/0009-0001-6988-7119","observed_orcids":["https://orcid.org/0009-0001-6988-7119"]},"institutions":[{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kayla Neyra","raw_affiliation_strings":["Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA"],"raw_orcid":"https://orcid.org/0009-0001-6988-7119","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA","institution_ids":["https://openalex.org/I58956616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103586051","display_name":"Diana M. Lopez","orcid":"https://orcid.org/0000-0003-0817-6951","observed_orcids":["https://orcid.org/0000-0003-0817-6951","https://orcid.org/0000-0001-6335-9625"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Diana M. Lopez","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA","Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA","Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0000-0003-0817-6951","affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052830237","display_name":"Ruo-Wen Chen","orcid":"https://orcid.org/0000-0003-2101-6944","observed_orcids":["https://orcid.org/0000-0003-2101-6944","https://orcid.org/0000-0001-9719-0784"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruo-Wen Chen","raw_affiliation_strings":["Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA"],"raw_orcid":"https://orcid.org/0000-0003-2101-6944","affiliations":[{"raw_affiliation_string":"Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093892549","display_name":"Deepta Paramasamy","orcid":"https://orcid.org/0009-0007-7779-918X","observed_orcids":["https://orcid.org/0009-0007-7779-918X"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]},{"id":"https://openalex.org/I55732556","display_name":"Arizona State University","ror":"https://ror.org/03efmqc40","country_code":"US","type":"education","lineage":["https://openalex.org/I55732556"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deepta Paramasamy","raw_affiliation_strings":["Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, 85281, USA"],"raw_orcid":"https://orcid.org/0009-0007-7779-918X","affiliations":[{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, 85281, USA","institution_ids":["https://openalex.org/I55732556"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130396186","display_name":"Quincy Bizjak","orcid":"https://orcid.org/0009-0007-1156-3019","observed_orcids":["https://orcid.org/0009-0007-1156-3019"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Quincy Bizjak","raw_affiliation_strings":["Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0009-0007-1156-3019","affiliations":[{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026440424","display_name":"Patrick D. Halley","orcid":"https://orcid.org/0000-0003-4942-8908","observed_orcids":["https://orcid.org/0000-0003-4942-8908"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrick D. Halley","raw_affiliation_strings":["Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0000-0003-4942-8908","affiliations":[{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057318574","display_name":"Yin Wei","orcid":"https://orcid.org/0000-0001-9915-5317","observed_orcids":["https://orcid.org/0000-0001-9915-5317"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yin Wei","raw_affiliation_strings":["Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0000-0001-9915-5317","affiliations":[{"raw_affiliation_string":"Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036622432","display_name":"Marcos M. Sotomayor","orcid":"https://orcid.org/0000-0002-3333-1805","observed_orcids":["https://orcid.org/0000-0002-3333-1805"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcos Sotomayor","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA"],"raw_orcid":"https://orcid.org/0000-0002-3333-1805","affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076813132","display_name":"Michael Guy Poirier","orcid":"https://orcid.org/0000-0002-1563-5792","observed_orcids":["https://orcid.org/0000-0002-1563-5792"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael G. Poirier","raw_affiliation_strings":["Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA","Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA","Department of Physics, The Ohio State University, Columbus, OH 43210, USA","Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA"],"raw_orcid":"https://orcid.org/0000-0002-1563-5792","affiliations":[{"raw_affiliation_string":"Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Department of Physics, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, 43212, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073477299","display_name":"Divita Mathur","orcid":"https://orcid.org/0000-0002-3537-7292","observed_orcids":["https://orcid.org/0000-0002-3537-7292"]},"institutions":[{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Divita Mathur","raw_affiliation_strings":["Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA"],"raw_orcid":"https://orcid.org/0000-0002-3537-7292","affiliations":[{"raw_affiliation_string":"Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA","institution_ids":["https://openalex.org/I58956616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090710614","display_name":"Carlos Ernesto Castro","orcid":"https://orcid.org/0000-0001-7023-6105","observed_orcids":["https://orcid.org/0000-0001-7023-6105"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carlos E. Castro","raw_affiliation_strings":["Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA","Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0000-0001-7023-6105","affiliations":[{"raw_affiliation_string":"Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070744700","display_name":"Wolfgang G. Pfeifer","orcid":"https://orcid.org/0000-0002-5589-8415","observed_orcids":["https://orcid.org/0000-0002-5589-8415"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Wolfgang G. Pfeifer","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA","Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","Department of Physics, The Ohio State University, Columbus, OH 43210, USA"],"raw_orcid":"https://orcid.org/0000-0002-5589-8415","affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology and Center for Mechanical Excitability, The University of Chicago, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]},{"raw_affiliation_string":"Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"Department of Physics, The Ohio State University, Columbus, OH 43210, USA","institution_ids":["https://openalex.org/I52357470"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5070744700"],"corresponding_institution_ids":["https://openalex.org/I40347166","https://openalex.org/I52357470"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12029","display_name":"DNA and Biological Computing","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-origami","display_name":"DNA origami","score":0.9710000157356262},{"id":"https://openalex.org/keywords/supercoiled-dna","display_name":"supercoiled DNA","score":0.9359999895095825},{"id":"https://openalex.org/keywords/linear-dna","display_name":"linear DNA","score":0.7710000276565552},{"id":"https://openalex.org/keywords/nanodevices","display_name":"nanodevices","score":0.5379999876022339}],"concepts":[{"id":"https://openalex.org/C118630614","wikidata":"https://www.wikidata.org/wiki/Q1154606","display_name":"DNA origami","level":3,"score":0.9221000075340271},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6485000252723694},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.5978000164031982},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.5756000280380249},{"id":"https://openalex.org/C101468663","wikidata":"https://www.wikidata.org/wiki/Q1620158","display_name":"Modular design","level":2,"score":0.5001999735832214},{"id":"https://openalex.org/C89429830","wikidata":"https://www.wikidata.org/wiki/Q735710","display_name":"Scaffold","level":2,"score":0.48590001463890076},{"id":"https://openalex.org/C129275984","wikidata":"https://www.wikidata.org/wiki/Q602847","display_name":"DNA nanotechnology","level":3,"score":0.45899999141693115},{"id":"https://openalex.org/C2776545253","wikidata":"https://www.wikidata.org/wiki/Q5464292","display_name":"Folding (DSP implementation)","level":2,"score":0.42500001192092896},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.41929998993873596},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4140999913215637},{"id":"https://openalex.org/C82714645","wikidata":"https://www.wikidata.org/wiki/Q438331","display_name":"Template","level":2,"score":0.38029998540878296},{"id":"https://openalex.org/C158456008","wikidata":"https://www.wikidata.org/wiki/Q412639","display_name":"DNA supercoil","level":4,"score":0.3686000108718872},{"id":"https://openalex.org/C191961535","wikidata":"https://www.wikidata.org/wiki/Q2360205","display_name":"A-DNA","level":3,"score":0.3540000021457672},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34709998965263367},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.32030001282691956},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.31360000371932983},{"id":"https://openalex.org/C3020215258","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Double stranded","level":3,"score":0.28279998898506165},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2678999900817871}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.03.19.709872","doi":"https://doi.org/10.64898/2026.03.19.709872","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.19.709872","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/23/2026.03.19.709872.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:13041984","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13041984/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13041984/pdf/nihpp-2026.03.19.709872v1.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv","raw_type":"Text"},{"id":"pmid:41929115","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41929115","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.64898/2026.03.19.709872","doi":"https://doi.org/10.64898/2026.03.19.709872","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.03.19.709872","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/03/23/2026.03.19.709872.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2487199047","display_name":null,"funder_award_id":"DMR-1420451","funder_id":"https://openalex.org/F4320309374","funder_display_name":"Ohio State University"},{"id":"https://openalex.org/G2561007569","display_name":"NSF-ANR MCB/PHY: DNA Origami-based machines for epigenetic manipulation of gene transcription","funder_award_id":"2411725","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G278275610","display_name":"CAREER: Structural Implications of Nucleic Acid Nanoparticles on their Transcription, Translation, and Transport","funder_award_id":"2439298","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G2904776157","display_name":"EFRI CEE: DNA origami tools to engineer chromatin structure and function in live cells","funder_award_id":"1933344","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G3723821865","display_name":null,"funder_award_id":"DMR-1420451","funder_id":"https://openalex.org/F4320337367","funder_display_name":"Division of Materials Research"},{"id":"https://openalex.org/G4486080083","display_name":"Center for Emergent Materials","funder_award_id":"1420451","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G5421835035","display_name":"Cytosolic Access and Instability of DNA nanoparticles","funder_award_id":"R00EB030013","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G6012531032","display_name":null,"funder_award_id":"DMR-1420451","funder_id":"https://openalex.org/F4320333422","funder_display_name":"Materials Research Science and Engineering Center, Harvard University"},{"id":"https://openalex.org/G6138233708","display_name":"Atomic Force Microscope","funder_award_id":"1s10od025096-01a1","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6336286406","display_name":null,"funder_award_id":"1420451","funder_id":"https://openalex.org/F4320337367","funder_display_name":"Division of Materials Research"},{"id":"https://openalex.org/G6451832128","display_name":"DMREF/Collaborative Research: DNA-based Sensing, Communicating, and Phase-Separating Materials","funder_award_id":"1921881","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G7540121869","display_name":null,"funder_award_id":"1933344","funder_id":"https://openalex.org/F4320337395","funder_display_name":"Division of Emerging Frontiers in Research and Innovation"},{"id":"https://openalex.org/G9000870326","display_name":"Mechanisms of chromatin regulation of transcription","funder_award_id":"R35GM139564","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309374","display_name":"Ohio State University","ror":"https://ror.org/00rs6vg23"},{"id":"https://openalex.org/F4320310281","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320333422","display_name":"Materials Research Science and Engineering Center, Harvard University","ror":"https://ror.org/01qa5ep47"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"},{"id":"https://openalex.org/F4320337367","display_name":"Division of Materials Research","ror":"https://ror.org/01pc7k308"},{"id":"https://openalex.org/F4320337390","display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","ror":"https://ror.org/0471zv972"},{"id":"https://openalex.org/F4320337395","display_name":"Division of Emerging Frontiers in Research and Innovation","ror":"https://ror.org/0388pet74"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7140102899.pdf","grobid_xml":"https://content.openalex.org/works/W7140102899.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4391657860","https://openalex.org/W1895035331","https://openalex.org/W3112384820","https://openalex.org/W2858548253","https://openalex.org/W1971630118","https://openalex.org/W4415531059","https://openalex.org/W2895926601","https://openalex.org/W2809815637","https://openalex.org/W7154424566","https://openalex.org/W4214538417"],"abstract_inverted_index":{"Scaffolded":[0],"DNA":[1,25,37,87,163,187],"origami":[2,26,164,188],"has":[3],"become":[4],"a":[5,34,48,96,107,176],"valuable":[6],"nanoscale":[7],"tool":[8],"for":[9,98,193],"applications":[10],"in":[11,175],"biomedical":[12],"and":[13,21,42,65,74,81,91,137,143,172,185],"physical":[14],"sciences.":[15],"Critical":[16],"to":[17,30,63,79,120,148,153],"leveraging":[18],"the":[19,31,83,99,121,126,191],"modular":[20],"programmable":[22],"properties":[23],"of":[24,39,85,102,109,140,162],"nanodevices":[27],"is":[28,45],"access":[29],"scaffold":[32,128],"strand,":[33,123],"long":[35,67],"single-stranded":[36],"(ssDNA)":[38],"precise":[40],"length":[41],"sequence,":[43],"which":[44],"folded":[46],"into":[47],"compact":[49],"shape":[50],"via":[51],"piecewise":[52],"base-pairing":[53],"with":[54],"many":[55],"staple":[56,173],"strands,":[57],"short":[58],"ssDNA":[59,68,104,133],"oligonucleotides.":[60],"Current":[61],"methods":[62,78],"produce":[64,80],"manipulate":[66,82],"scaffolds":[69],"can":[70],"be":[71],"costly,":[72],"time-consuming,":[73],"cumbersome.":[75],"In":[76,151],"contrast,":[77],"sequence":[84],"double-stranded":[86],"(dsDNA)":[88],"are":[89],"efficient":[90],"scalable.":[92],"Here,":[93],"we":[94,156,182],"present":[95],"method":[97],"rapid":[100],"isolation":[101,134],"target":[103,127],"sequences":[105,142],"from":[106,135,146,166],"variety":[108],"dsDNA":[110,167],"sources":[111],"using":[112,169],"oligonucleotides":[113],"as":[114],"blocking":[115,171],"strands":[116,174],"that":[117],"bind":[118],"continuously":[119],"undesired":[122],"thereby":[124],"releasing":[125],"strand.":[129],"We":[130],"report":[131],"successful":[132],"linear":[136],"supercoiled":[138],"dsDNAs":[139],"various":[141],"lengths,":[144],"ranging":[145],"769":[147],"15,101":[149],"nucleotides.":[150],"addition":[152],"isolating":[154],"ssDNA,":[155],"demonstrated":[157],"this":[158],"approach":[159],"enables":[160],"folding":[161],"directly":[165],"templates":[168],"both":[170],"single-pot":[177],"thermally":[178],"controlled":[179],"reaction.":[180],"Furthermore,":[181],"explore":[183],"multi-scaffold":[184],"gene-encoding":[186],"structures,":[189],"expanding":[190],"framework":[192],"application-based":[194],"designs.":[195]},"counts_by_year":[],"updated_date":"2026-10-29T09:56:32.521200","created_date":"2026-03-24T00:00:00"}