{"id":"https://openalex.org/W7140085697","doi":"https://doi.org/10.64898/2026.03.20.713238","title":"Quantifying Drosophila Feeding Behavior Using flyPAD and optoPAD","display_name":"Quantifying Drosophila Feeding Behavior Using flyPAD and optoPAD","publication_year":2026,"publication_date":"2026-03-23","ids":{"openalex":"https://openalex.org/W7140085697","doi":"https://doi.org/10.64898/2026.03.20.713238","pmid":"https://pubmed.ncbi.nlm.nih.gov/41928927","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13041994"},"language":"en","primary_location":{"id":"doi:10.64898/2026.03.20.713238","doi":"https://doi.org/10.64898/2026.03.20.713238","is_oa":false,"landing_page_url":"https://doi.org/10.64898/2026.03.20.713238","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13041994/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029937338","display_name":"Nicholas J. Collins","orcid":"https://orcid.org/0000-0001-8536-170X","observed_orcids":["https://orcid.org/0000-0001-8536-170X"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas J. Collins","raw_affiliation_strings":["Department of Biological Sciences, University of Delaware, Newark, DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark, DE","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130327120","display_name":"Madison N. Endres","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Madison Endres","raw_affiliation_strings":["Department of Biological Sciences, University of Delaware, Newark, DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark, DE","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109999021","display_name":"I.G. Sinakevitch","orcid":"https://orcid.org/0000-0002-2771-3823","observed_orcids":["https://orcid.org/0000-0002-2771-3823"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Irina Sinakevitch","raw_affiliation_strings":["Department of Biological Sciences, University of Delaware, Newark, DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark, DE","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003989514","display_name":"Lisha Shao","orcid":"https://orcid.org/0000-0001-5128-7548","observed_orcids":["https://orcid.org/0000-0001-5128-7548"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lisha Shao","raw_affiliation_strings":["Department of Biological Sciences, University of Delaware, Newark, DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Delaware, Newark, DE","institution_ids":["https://openalex.org/I86501945"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5003989514"],"corresponding_institution_ids":["https://openalex.org/I86501945"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10489","display_name":"Regulation of Appetite and Obesity","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2807","display_name":"Endocrine and Autonomic Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10676","display_name":"Genetics, Aging, and Longevity in Model Organisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1302","display_name":"Aging"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/food-consumption","display_name":"dietary intake","score":0.492000013589859},{"id":"https://openalex.org/keywords/food-choice","display_name":"food choice","score":0.9900000095367432},{"id":"https://openalex.org/keywords/gustatory-stimuli","display_name":"gustatory stimuli","score":0.8069999814033508},{"id":"https://openalex.org/keywords/optogenetic-stimulation","display_name":"optogenetic stimulation","score":0.9629999995231628},{"id":"https://openalex.org/keywords/closed-loop-optogenetics","display_name":"closed-loop optogenetics","score":0.8859999775886536}],"concepts":[{"id":"https://openalex.org/C2780104201","wikidata":"https://www.wikidata.org/wiki/Q130888","display_name":"Drosophila melanogaster","level":3,"score":0.8616999983787537},{"id":"https://openalex.org/C50738837","wikidata":"https://www.wikidata.org/wiki/Q781492","display_name":"Optogenetics","level":2,"score":0.8016999959945679},{"id":"https://openalex.org/C94487597","wikidata":"https://www.wikidata.org/wiki/Q11101","display_name":"Sensory system","level":2,"score":0.6481999754905701},{"id":"https://openalex.org/C3017650416","wikidata":"https://www.wikidata.org/wiki/Q3281331","display_name":"Feeding behavior","level":2,"score":0.6108999848365784},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6078000068664551},{"id":"https://openalex.org/C2776041557","wikidata":"https://www.wikidata.org/wiki/Q3715451","display_name":"Drosophila (subgenus)","level":3,"score":0.487199991941452},{"id":"https://openalex.org/C3018685816","wikidata":"https://www.wikidata.org/wiki/Q213449","display_name":"Food intake","level":2,"score":0.43650001287460327},{"id":"https://openalex.org/C19843653","wikidata":"https://www.wikidata.org/wiki/Q213907","display_name":"Model organism","level":3,"score":0.4120999872684479},{"id":"https://openalex.org/C2776998849","wikidata":"https://www.wikidata.org/wiki/Q3766082","display_name":"Melanogaster","level":4,"score":0.4011000096797943},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.38429999351501465},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.31040000915527344},{"id":"https://openalex.org/C2987526018","wikidata":"https://www.wikidata.org/wiki/Q213449","display_name":"Food consumption","level":2,"score":0.3100999891757965},{"id":"https://openalex.org/C2984127161","wikidata":"https://www.wikidata.org/wiki/Q969316","display_name":"Neural activity","level":2,"score":0.3089999854564667},{"id":"https://openalex.org/C2776030549","wikidata":"https://www.wikidata.org/wiki/Q7829395","display_name":"Nutrient sensing","level":3,"score":0.296999990940094},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.28679999709129333},{"id":"https://openalex.org/C2988540567","wikidata":"https://www.wikidata.org/wiki/Q12070446","display_name":"Model system","level":2,"score":0.28529998660087585}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.03.20.713238","doi":"https://doi.org/10.64898/2026.03.20.713238","is_oa":false,"landing_page_url":"https://doi.org/10.64898/2026.03.20.713238","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:41928927","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41928927","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13041994","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13041994/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:13041994","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13041994/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000882154","display_name":"Elucidating the molecular and neural structure of female sexual reward","funder_award_id":"R35GM147504","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":26,"referenced_works":["https://openalex.org/W1975460874","https://openalex.org/W1987942547","https://openalex.org/W2007299852","https://openalex.org/W2007301606","https://openalex.org/W2014079380","https://openalex.org/W2049741320","https://openalex.org/W2055646225","https://openalex.org/W2060951467","https://openalex.org/W2114570000","https://openalex.org/W2127051362","https://openalex.org/W2129103583","https://openalex.org/W2157765869","https://openalex.org/W2296826911","https://openalex.org/W2508791255","https://openalex.org/W2802376333","https://openalex.org/W2949443843","https://openalex.org/W3184608991","https://openalex.org/W3195722571","https://openalex.org/W4240203055","https://openalex.org/W4283826056","https://openalex.org/W4293688054","https://openalex.org/W4302362788","https://openalex.org/W4385463069","https://openalex.org/W4387128679","https://openalex.org/W4399532688","https://openalex.org/W4404392720"],"related_works":["https://openalex.org/W7169849953","https://openalex.org/W2949443843","https://openalex.org/W2801561012","https://openalex.org/W4412363620","https://openalex.org/W2964345886","https://openalex.org/W2185030619","https://openalex.org/W4200412761","https://openalex.org/W4287118982","https://openalex.org/W7204104063","https://openalex.org/W2001681532"],"abstract_inverted_index":{"Summary":[0],"Quantifying":[1],"feeding":[2,53,102,123,154],"behavior":[3],"with":[4,85,137],"high":[5],"temporal":[6],"and":[7,19,25,37,65,81,98,104,112,140,152,161],"spatial":[8],"precision":[9],"is":[10,90],"critical":[11],"for":[12,34,72,92,109,130,147],"understanding":[13],"how":[14],"internal":[15],"state,":[16],"sensory":[17],"cues,":[18],"neural":[20,134],"activity":[21],"shape":[22],"food":[23,74],"intake":[24],"dietary":[26,38],"choice.":[27],"Here,":[28],"we":[29],"describe":[30],"a":[31],"detailed":[32],"protocol":[33],"performing":[35],"consumption":[36],"choice":[39,114],"assays":[40],"in":[41],"Drosophila":[42,122],"using":[43],"the":[44],"flyPAD/optoPAD":[45],"system.":[46],"This":[47,88],"method":[48],"enables":[49],"simultaneous":[50],"measurement":[51,120],"of":[52,62,101,121,127,133],"events":[54],"across":[55],"multiple":[56],"arenas":[57],"while":[58],"allowing":[59],"precise":[60,131],"control":[61,132],"gustatory":[63],"stimuli":[64],"optogenetic":[66,110,128,142],"stimulation.":[67],"We":[68],"provide":[69],"step-by-step":[70],"instructions":[71],"assay":[73],"preparation,":[75],"flyPAD":[76],"arena":[77,159],"setup,":[78],"data":[79,83],"acquisition,":[80],"downstream":[82],"organization":[84],"suggested":[86],"analyses.":[87],"approach":[89],"suitable":[91],"studying":[93],"consumption,":[94,148],"nutrient":[95,149],"preference,":[96,150],"learning,":[97,151],"state-dependent":[99,153],"modulation":[100],"behaviors,":[103],"can":[105],"be":[106],"readily":[107],"adapted":[108],"manipulations":[111],"comparative":[113],"assays.":[115],"Highlights":[116],"High":[117],"throughput,":[118],"quantitative":[119],"behavior.":[124],"Easy":[125],"integration":[126],"stimulation":[129,143],"circuits.":[135],"Compatible":[136],"both":[138],"open-loop":[139],"closed-loop":[141],"paradigms.":[144],"Versatile":[145],"applications":[146],"behaviors.":[155],"Step-by-step":[156],"guidance":[157],"from":[158],"acquisition":[160],"calibration":[162],"through":[163],"statistical":[164],"modeling.":[165]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2026-03-24T00:00:00"}