{"id":"https://openalex.org/W7129645751","doi":"https://doi.org/10.64898/2026.02.13.704926","title":"CaptureBody \u2013 an anti-CD45 x anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry","display_name":"CaptureBody \u2013 an anti-CD45 x anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry","publication_year":2026,"publication_date":"2026-02-16","ids":{"openalex":"https://openalex.org/W7129645751","doi":"https://doi.org/10.64898/2026.02.13.704926","pmid":"https://pubmed.ncbi.nlm.nih.gov/41756882"},"language":"en","primary_location":{"id":"doi:10.64898/2026.02.13.704926","doi":"https://doi.org/10.64898/2026.02.13.704926","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.02.13.704926","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.02.13.704926","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5092792816","display_name":"Alexander E. Zambidis","orcid":"https://orcid.org/0009-0009-4869-1983","observed_orcids":["https://orcid.org/0009-0009-4869-1983"]},"institutions":[{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander E. Zambidis","raw_affiliation_strings":["Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"],"raw_orcid":"https://orcid.org/0009-0009-4869-1983","affiliations":[{"raw_affiliation_string":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA","institution_ids":["https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019779385","display_name":"Latha Kallur Siddaramaiah","orcid":"https://orcid.org/0009-0008-4998-3913","observed_orcids":["https://orcid.org/0009-0008-4998-3913"]},"institutions":[{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Latha Kallur Siddaramaiah","raw_affiliation_strings":["Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"],"raw_orcid":"https://orcid.org/0009-0008-4998-3913","affiliations":[{"raw_affiliation_string":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA","institution_ids":["https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083504283","display_name":"A Konecny","orcid":"https://orcid.org/0000-0002-3492-1006","observed_orcids":["https://orcid.org/0000-0002-3492-1006"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew J. Konecny","raw_affiliation_strings":["Department of Immunology, University of Washington, Seattle, WA, USA","Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"],"raw_orcid":"https://orcid.org/0000-0002-3492-1006","affiliations":[{"raw_affiliation_string":"Department of Immunology, University of Washington, Seattle, WA, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA","institution_ids":["https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022673921","display_name":"Matthew D. Gray","orcid":"https://orcid.org/0000-0002-4260-9784","observed_orcids":["https://orcid.org/0000-0002-4260-9784"]},"institutions":[{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew Gray","raw_affiliation_strings":["Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"],"raw_orcid":"https://orcid.org/0000-0002-4260-9784","affiliations":[{"raw_affiliation_string":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA","institution_ids":["https://openalex.org/I4210089486"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071870162","display_name":"Martin Prlic","orcid":"https://orcid.org/0000-0002-0685-9321","observed_orcids":["https://orcid.org/0000-0002-0685-9321"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin Prlic","raw_affiliation_strings":["Department of Global Health, University of Washington, Seattle, WA, USA","Department of Immunology, University of Washington, Seattle, WA, USA","Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"],"raw_orcid":"https://orcid.org/0000-0002-0685-9321","affiliations":[{"raw_affiliation_string":"Department of Global Health, University of Washington, Seattle, WA, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Immunology, University of Washington, Seattle, WA, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA","institution_ids":["https://openalex.org/I4210089486"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9535999894142151,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.9535999894142151,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.023099999874830246,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.010599999688565731,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bispecific-antibodies","display_name":"bispecific antibodies","score":0.9760000109672546},{"id":"https://openalex.org/keywords/anti-igg-antibodies","display_name":"anti-IgG antibodies","score":0.9449999928474426},{"id":"https://openalex.org/keywords/spectral-unmixing","display_name":"spectral unmixing","score":0.8840000033378601},{"id":"https://openalex.org/keywords/spectral-flow-cytometry","display_name":"spectral flow cytometry","score":0.8299999833106995},{"id":"https://openalex.org/keywords/compensation","display_name":"compensation","score":0.49799999594688416},{"id":"https://openalex.org/keywords/nanobodies","display_name":"nanobodies","score":0.8690000176429749}],"concepts":[{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.7569000124931335},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.5411999821662903},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.46970000863075256},{"id":"https://openalex.org/C2781294515","wikidata":"https://www.wikidata.org/wiki/Q2733470","display_name":"Stain","level":3,"score":0.45899999141693115},{"id":"https://openalex.org/C2780023022","wikidata":"https://www.wikidata.org/wiki/Q1338171","display_name":"Compensation (psychology)","level":2,"score":0.4408000111579895},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.387800008058548},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.3723999857902527},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.36890000104904175},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34549999237060547},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.32670000195503235},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3237000107765198},{"id":"https://openalex.org/C2776950831","wikidata":"https://www.wikidata.org/wiki/Q15635432","display_name":"Single-cell analysis","level":3,"score":0.322299987077713},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3151000142097473},{"id":"https://openalex.org/C2983668108","wikidata":"https://www.wikidata.org/wiki/Q280453","display_name":"Spectral analysis","level":3,"score":0.3091000020503998},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3061000108718872},{"id":"https://openalex.org/C38349280","wikidata":"https://www.wikidata.org/wiki/Q1434290","display_name":"Flow (mathematics)","level":2,"score":0.3043999969959259},{"id":"https://openalex.org/C3020495827","wikidata":"https://www.wikidata.org/wiki/Q426141","display_name":"Bispecific antibody","level":4,"score":0.3019999861717224},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2971000075340271},{"id":"https://openalex.org/C3017456870","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Specific antibody","level":3,"score":0.2867000102996826},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.2800999879837036},{"id":"https://openalex.org/C4839761","wikidata":"https://www.wikidata.org/wiki/Q212111","display_name":"Spectral line","level":2,"score":0.27559998631477356},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.2702000141143799},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.2500999867916107}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.64898/2026.02.13.704926","doi":"https://doi.org/10.64898/2026.02.13.704926","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.02.13.704926","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:41756882","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41756882","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12934800","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12934800/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.64898/2026.02.13.704926","doi":"https://doi.org/10.64898/2026.02.13.704926","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.02.13.704926","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7444698384","display_name":"The role of tissue-resident T cells in resolving inflammation of the human oral mucosa","funder_award_id":"1r56de032009-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000880613","display_name":"Cancer Center Support Grant","funder_award_id":"P30CA015704","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000885975","display_name":"Metabolite- and cytokine-mediated signals interact to control human CD8 T cell responses in tissues","funder_award_id":"1r01ai179712-01a1","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000903294","display_name":"Inflammation-driven T Cell Responses and their Dichotomous Effect on Host Immunity","funder_award_id":"R01AI123323","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W33263437","https://openalex.org/W1576593419","https://openalex.org/W1585011232","https://openalex.org/W1604897966","https://openalex.org/W1951089650","https://openalex.org/W2020368956","https://openalex.org/W2050207461","https://openalex.org/W2055290772","https://openalex.org/W2062093709","https://openalex.org/W2091622299","https://openalex.org/W2119336981","https://openalex.org/W2130314959","https://openalex.org/W2159917050","https://openalex.org/W2160342295","https://openalex.org/W2165499430","https://openalex.org/W2169610187","https://openalex.org/W2320044375","https://openalex.org/W2571138221","https://openalex.org/W2766254284","https://openalex.org/W2910931863","https://openalex.org/W2948141873","https://openalex.org/W2981698047","https://openalex.org/W3009940849","https://openalex.org/W3080263813","https://openalex.org/W3082503354","https://openalex.org/W3197394107","https://openalex.org/W4229020479","https://openalex.org/W4280507626","https://openalex.org/W4285589485","https://openalex.org/W4308125665","https://openalex.org/W4377564707","https://openalex.org/W4378083369","https://openalex.org/W4387114623","https://openalex.org/W4387164156","https://openalex.org/W4393042616","https://openalex.org/W4394919069","https://openalex.org/W4402638771","https://openalex.org/W4403784265","https://openalex.org/W4403825811","https://openalex.org/W4408528285","https://openalex.org/W4411466638","https://openalex.org/W4413432081","https://openalex.org/W4415602714","https://openalex.org/W7116700503"],"related_works":[],"abstract_inverted_index":{"Accurate":[0],"spectral":[1,66,141],"unmixing":[2,67],"is":[3,41,49],"a":[4,14,38,46,90,95,134],"critical":[5],"step":[6],"for":[7,18,32,129,140],"flow":[8,142],"cytometry":[9,143],"data":[10],"analysis":[11],"and":[12,98,113,126],"requires":[13],"single":[15,34],"stain":[16,35],"control":[17],"every":[19],"fluorescent":[20],"parameter":[21],"used":[22,31],"in":[23,58,65],"an":[24],"experiment.":[25],"Currently,":[26],"compensation":[27,54,69],"particles":[28,55],"are":[29],"often":[30],"making":[33],"controls":[36],"when":[37],"target":[39,82],"protein":[40],"of":[42,50,75,78,81,117,133],"low":[43,51],"abundance":[44,80],"or":[45,68,84],"cell":[47,86],"type":[48],"frequency.":[52],"However,":[53],"introduce":[56],"incongruencies":[57],"emission":[59],"spectra":[60],"compared":[61],"to":[62,105,136],"cells":[63,76],"resulting":[64],"errors.":[70],"To":[71],"enable":[72],"the":[73,79,115,130],"use":[74,139],"regardless":[77],"proteins":[83],"immune":[85],"type,":[87],"we":[88],"generated":[89],"bispecific":[91,108],"antibody":[92],"that":[93],"links":[94],"human":[96],"anti-CD45":[97],"mouse":[99],"anti-IgG":[100],"variable":[101],"region.":[102],"We":[103,122],"refer":[104],"this":[106],"new":[107],"tool":[109],"as":[110],"CaptureBody":[111,135],"(CB)":[112],"highlight":[114],"benefits":[116],"its":[118],"final":[119],"nanobody-based":[120],"design.":[121],"provide":[123],"all":[124],"sequences":[125],"methods":[127],"necessary":[128],"in-house":[131],"expression":[132],"disseminate":[137],"their":[138],"experiments.":[144]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2026-02-18T00:00:00"}
