{"id":"https://openalex.org/W7128480759","doi":"https://doi.org/10.1177/170882679804600505","title":"Successful DNA Transfer in Cultured Human Mesangial Cells Using Replication Deficient Recombinant Adenovirus","display_name":"Successful DNA Transfer in Cultured Human Mesangial Cells Using Replication Deficient Recombinant Adenovirus","publication_year":1998,"publication_date":"1998-06-01","ids":{"openalex":"https://openalex.org/W7128480759","doi":"https://doi.org/10.1177/170882679804600505","mag":"2414366348","pmid":"https://pubmed.ncbi.nlm.nih.gov/9676052"},"language":"en","primary_location":{"id":"doi:10.1177/170882679804600505","doi":"https://doi.org/10.1177/170882679804600505","is_oa":false,"landing_page_url":"https://doi.org/10.1177/170882679804600505","pdf_url":null,"source":{"id":"https://openalex.org/S104415515","display_name":"Journal of Investigative Medicine","issn_l":"1081-5589","issn":["1081-5589","1708-8267"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Investigative Medicine","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061119336","display_name":"N. S. Nahman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N. Stanley Nahman","raw_affiliation_strings":["Departments of Internal Medicine and Pathology, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Internal Medicine and Pathology, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078328345","display_name":"Kelly Reed Clark","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K. Reed Clark","raw_affiliation_strings":["Department of Molecular Medicine, Children's Hospital Medical Center, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Medicine, Children's Hospital Medical Center, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080838490","display_name":"Thomas J. Sferra","orcid":"https://orcid.org/0000-0001-6893-9880","observed_orcids":["https://orcid.org/0000-0001-6893-9880"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas J. Sferra","raw_affiliation_strings":["Department of Molecular Medicine, Children's Hospital Medical Center, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Medicine, Children's Hospital Medical Center, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027315009","display_name":"Kathleen E. Urban","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kathleen E. Urban","raw_affiliation_strings":["Departments of Internal Medicine and Pathology, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Internal Medicine and Pathology, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5098855330","display_name":"Anne E. Troike","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anne E. Troike","raw_affiliation_strings":["Departments of Internal Medicine and Pathology, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Internal Medicine and Pathology, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066088104","display_name":"Julie C. Kronenberger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Julie Kronenberger","raw_affiliation_strings":["Departments of Internal Medicine and Pathology, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Internal Medicine and Pathology, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024780472","display_name":"Daniel D. Sedmak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel D. Sedmak","raw_affiliation_strings":["Departments of Internal Medicine and Pathology, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Internal Medicine and Pathology, The Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I52357470"],"apc_list":{"value":3900,"currency":"USD","value_usd":3900},"apc_paid":null,"fwci":0.571,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.69317248,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"46","issue":"5","first_page":"204","last_page":"209"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11029","display_name":"Renal Diseases and Glomerulopathies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mesangial-cells","display_name":"mesangial cells","score":0.8080000281333923},{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.6919999718666077},{"id":"https://openalex.org/keywords/gene-transfer","display_name":"gene transfer","score":0.7699999809265137},{"id":"https://openalex.org/keywords/replication-deficient-adenovirus","display_name":"replication-defective adenovirus","score":0.902999997138977},{"id":"https://openalex.org/keywords/lacz-reporter","display_name":"lacZ reporter","score":0.8339999914169312},{"id":"https://openalex.org/keywords/transduction-efficiency","display_name":"transduction efficiency","score":0.6420000195503235},{"id":"https://openalex.org/keywords/transgene-stability","display_name":"transgene stability","score":0.574999988079071}],"concepts":[{"id":"https://openalex.org/C38240268","wikidata":"https://www.wikidata.org/wiki/Q17125118","display_name":"Trypsinization","level":4,"score":0.8820000290870667},{"id":"https://openalex.org/C2779131090","wikidata":"https://www.wikidata.org/wiki/Q3331086","display_name":"Mesangial cell","level":3,"score":0.7275999784469604},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.7005000114440918},{"id":"https://openalex.org/C203233990","wikidata":"https://www.wikidata.org/wiki/Q1511135","display_name":"Multiplicity of infection","level":3,"score":0.6816999912261963},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6029999852180481},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5478000044822693},{"id":"https://openalex.org/C2776954459","wikidata":"https://www.wikidata.org/wiki/Q193447","display_name":"Adenoviridae","level":4,"score":0.5404999852180481},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.5327000021934509},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.5250999927520752},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.49779999256134033},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4943000078201294},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.45980000495910645},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.44609999656677246},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.42410001158714294},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.42250001430511475},{"id":"https://openalex.org/C86492073","wikidata":"https://www.wikidata.org/wiki/Q413766","display_name":"Fibronectin","level":3,"score":0.37450000643730164},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37220001220703125},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.3490999937057495},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.34459999203681946},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34439998865127563},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.33000001311302185},{"id":"https://openalex.org/C2781036101","wikidata":"https://www.wikidata.org/wiki/Q837054","display_name":"Adenovirus infection","level":3,"score":0.32589998841285706},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.31769999861717224},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3107999861240387},{"id":"https://openalex.org/C73247094","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Transgenesis","level":5,"score":0.2948000133037567},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.29019999504089355},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2775999903678894},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.2718999981880188},{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.2630999982357025},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2619999945163727},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.25949999690055847},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.25609999895095825}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005920","descriptor_name":"Glomerular Mesangium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005920","descriptor_name":"Glomerular Mesangium","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007763","descriptor_name":"Lac Operon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1177/170882679804600505","doi":"https://doi.org/10.1177/170882679804600505","is_oa":false,"landing_page_url":"https://doi.org/10.1177/170882679804600505","pdf_url":null,"source":{"id":"https://openalex.org/S104415515","display_name":"Journal of Investigative Medicine","issn_l":"1081-5589","issn":["1081-5589","1708-8267"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Investigative Medicine","raw_type":"journal-article"},{"id":"pmid:9676052","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9676052","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","raw_type":"Journal Article"},{"id":"mag:2414366348","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9676052","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9763000011444092,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W988054948","https://openalex.org/W1994092968","https://openalex.org/W2010018722","https://openalex.org/W2012055448","https://openalex.org/W2035960254","https://openalex.org/W2051758487","https://openalex.org/W2067198866","https://openalex.org/W2133388585","https://openalex.org/W2313269464","https://openalex.org/W2333807927"],"related_works":["https://openalex.org/W2081738353","https://openalex.org/W2026211946","https://openalex.org/W2078221752","https://openalex.org/W2388501391","https://openalex.org/W2415445296","https://openalex.org/W2063590231","https://openalex.org/W1964033324","https://openalex.org/W2179122767","https://openalex.org/W2012055448","https://openalex.org/W2751129854"],"abstract_inverted_index":{"BACKGROUND:":[0],"Efficient":[1],"transfer":[2,38,232],"of":[3,17,32,44,51,84,89,132,148,156,165,174,221,228,247],"DNA":[4,39,243],"into":[5,41,244],"human":[6,45,52,248],"mesangial":[7,21,46,53,85,91,123,149,210,249],"cells":[8,54,92,124,150],"is":[9,235],"an":[10,58,154,236],"essential":[11],"first":[12],"step":[13],"in":[14,103,125,171],"the":[15,25,30,172],"development":[16],"gene":[18],"therapies":[19],"for":[20,70,198,241],"cell-mediated":[22],"glomerulopathies.":[23],"In":[24],"present":[26],"studies,":[27],"we":[28],"assessed":[29,74,111],"ability":[31],"replication":[33],"deficient":[34],"recombinant":[35],"adenovirus":[36,234],"to":[37,139,196],"(transduce)":[40],"primary":[42,245],"cultures":[43,50,246],"cells.":[47,176,250],"METHODS:":[48],"Primary":[49],"were":[55,151,169,184],"transduced":[56,90,122,152,175],"with":[57,204,233],"adenoviral":[59],"vector":[60],"(rAv":[61],"beta-gal)":[62],"containing":[63],"a":[64,126,130],"CMVllacZ":[65],"promoter-reporter":[66],"expression":[67,83,96],"cassette":[68],"coding":[69],"beta-galactosidase":[71],"(beta-gal).":[72],"We":[73,116],"soluble":[75],"and":[76,81,105,183,216,223],"histologic":[77],"beta-gal":[78,120,206],"activity,":[79],"morphology,":[80],"phenotypic":[82],"cell":[86,211],"transductants,":[87],"durability":[88],"by":[93,112],"measuring":[94],"transgene":[95],"following":[97,108,201],"trypsinization":[98,222],"or":[99],"after":[100,190],"prolonged":[101],"periods":[102],"culture":[104],"metabolic":[106],"stability":[107],"transduction":[109],"(as":[110],"fibronectin":[113],"biosynthesis).":[114],"RESULTS:":[115],"showed":[117],"that":[118],"rAv":[119,205],"efficiently":[121],"dose-dependent":[127],"fashion":[128],"at":[129,153],"multiplicity":[131],"infectious":[133],"units":[134],"(MOI)":[135],"ranging":[136],"from":[137],"1":[138],"400":[140],"plaque":[141],"forming":[142],"units/cell":[143],"(pfu/cell).":[144],"One":[145],"hundred":[146],"percent":[147],"MOI":[155],"100":[157],"pfu/cell.":[158],"By":[159],"electron":[160],"microscopic":[161],"evaluation,":[162],"viral":[163],"particles":[164],"approximately":[166],"85-90":[167],"nm":[168],"demonstrated":[170],"cytoplasm":[173],"Following":[177],"transduction,":[178],"legal":[179],"levels":[180,189,194],"rose":[181],"rapidly":[182],"10-fold":[185],"greater":[186],"than":[187],"baseline":[188],"2":[191],"hours.":[192],"Beta-gal":[193],"continued":[195],"rise":[197],"7":[199],"days":[200],"transduction.":[202],"Transduction":[203],"was":[207],"well":[208,238],"tolerated;":[209],"transductants":[212],"maintained":[213,224],"normal":[214,225],"morphology":[215],"phenotype,":[217],"tolerated":[218,239],"3":[219],"cycles":[220],"constitutive":[226],"production":[227],"fibronectin.":[229],"CONCLUSIONS:":[230],"Gene":[231],"effective,":[237],"approach":[240],"introducing":[242]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2026-02-11T00:00:00"}
