{"id":"https://openalex.org/W7127089077","doi":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","title":"Valida\u00e7\u00e3o da sonda para o gene SRY para estudo de quimerismo sexual em transplantados de medula \u00f3ssea, utilizando a metodologia de FISH automatizado","display_name":"Valida\u00e7\u00e3o da sonda para o gene SRY para estudo de quimerismo sexual em transplantados de medula \u00f3ssea, utilizando a metodologia de FISH automatizado","publication_year":2025,"publication_date":"2025-01-01","ids":{"openalex":"https://openalex.org/W7127089077","doi":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722"},"language":"pt","primary_location":{"id":"doi:10.5327/1516-3180.cbpc/ml.15722","doi":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","is_oa":true,"landing_page_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","pdf_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"S\u00e3o Paulo Medical Journal","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078833098","display_name":"Daniela Borri","orcid":"https://orcid.org/0000-0003-4543-4905","observed_orcids":["https://orcid.org/0000-0003-4543-4905"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Daniela Borri","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124848571","display_name":"Lauren Ingrid Santos Moreira da Concei\u00e7\u00e3o","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lauren Ingrid Santos Moreira da Concei\u00e7\u00e3o","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007536609","display_name":"Roberta Maria da Silva Oliveira Safranauskas","orcid":"https://orcid.org/0000-0003-0022-0161","observed_orcids":["https://orcid.org/0000-0003-0022-0161"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roberta Maria da Silva Oliveira Safranauskas","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072254224","display_name":"Maria Gabriella Cordeiro","orcid":"https://orcid.org/0000-0002-8610-6716","observed_orcids":["https://orcid.org/0000-0002-8610-6716"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Maria Gabriella Cordeiro","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073753453","display_name":"Renata Kiyomi Kishimoto","orcid":"https://orcid.org/0000-0002-0900-3510","observed_orcids":["https://orcid.org/0000-0002-0900-3510"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Renata Kiyomi Kishimoto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109588351","display_name":"Juan Santos E Silva","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jennyffer Gabrielly Silva","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124784253","display_name":"Jefferson Correia de Ara\u00fajo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181391015","display_name":"Universidade Estadual de Campinas (UNICAMP)","ror":"https://ror.org/04wffgt70","country_code":"BR","type":"education","lineage":["https://openalex.org/I181391015"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Jefferson Correia de Ara\u00fajo","raw_affiliation_strings":["Universidade Estadual de Campinas ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade Estadual de Campinas ,","institution_ids":["https://openalex.org/I181391015"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077322577","display_name":"Elvira Deolinda Rodrigues Pereira Velloso","orcid":"https://orcid.org/0000-0002-9707-0579","observed_orcids":["https://orcid.org/0000-0002-9707-0579"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Elvira Deolinda Rodrigues Pereira Velloso","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"117","last_page":"117"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9731000065803528,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11077","display_name":"Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities","score":0.008500000461935997,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11213","display_name":"Genomic variations and chromosomal abnormalities","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sry-gene","display_name":"SRY gene","score":0.7770000100135803},{"id":"https://openalex.org/keywords/bone-marrow-transplantation","display_name":"bone marrow transplantation","score":0.6890000104904175},{"id":"https://openalex.org/keywords/hematopoietic-stem-cell-transplantation","display_name":"hematopoietic stem cell transplantation","score":0.8920000195503235},{"id":"https://openalex.org/keywords/microsatellite-markers","display_name":"microsatellite markers","score":0.5329999923706055},{"id":"https://openalex.org/keywords/karyotype","display_name":"karyotype","score":0.8360000252723694},{"id":"https://openalex.org/keywords/reference-values","display_name":"reference values","score":0.5220000147819519},{"id":"https://openalex.org/keywords/chimerism-monitoring","display_name":"chimerism monitoring","score":0.6200000047683716},{"id":"https://openalex.org/keywords/hybridization-efficiency","display_name":"hybridization efficiency","score":0.6269999742507935},{"id":"https://openalex.org/keywords/sensitivity-and-specificity","display_name":"sensitivity and specificity","score":0.6570000052452087}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6140000224113464},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5753999948501587},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.5512999892234802},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.536300003528595},{"id":"https://openalex.org/C73456138","wikidata":"https://www.wikidata.org/wiki/Q409114","display_name":"Testis determining factor","level":4,"score":0.4041000008583069},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.2662999927997589},{"id":"https://openalex.org/C2777542201","wikidata":"https://www.wikidata.org/wiki/Q1336182","display_name":"Fluorescence in situ hybridization","level":4,"score":0.19020000100135803},{"id":"https://openalex.org/C2993290233","wikidata":"https://www.wikidata.org/wiki/Q608","display_name":"Sexual behavior","level":2,"score":0.16850000619888306},{"id":"https://openalex.org/C2989421709","wikidata":"https://www.wikidata.org/wiki/Q6502151","display_name":"Tax evasion","level":2,"score":0.15880000591278076},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.15770000219345093}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.5327/1516-3180.cbpc/ml.15722","doi":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","is_oa":true,"landing_page_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","pdf_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"S\u00e3o Paulo Medical Journal","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.5327/1516-3180.cbpc/ml.15722","doi":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","is_oa":true,"landing_page_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","pdf_url":"https://doi.org/10.5327/1516-3180.cbpc/ml.15722","source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"S\u00e3o Paulo Medical Journal","raw_type":"proceedings-article"},"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7127089077.pdf","grobid_xml":"https://content.openalex.org/works/W7127089077.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4415670569","https://openalex.org/W7127178213","https://openalex.org/W2428901951","https://openalex.org/W1965000271","https://openalex.org/W7000904049","https://openalex.org/W2171839304","https://openalex.org/W2027879640","https://openalex.org/W7046648902","https://openalex.org/W2995271852","https://openalex.org/W2066699756"],"abstract_inverted_index":{"Introdu\u00e7\u00e3o:":[0],"A":[1,175,192,209,252],"determina\u00e7\u00e3o":[2],"do":[3,74,87,89,98,127,220,248,263],"quimerismo":[4,119,162,274],"sexual":[5],"p\u00f3s-transplante":[6],"de":[7,53,67,114,118,125,143,146,150,155,168,181,184,211,225,228,236,241,273],"c\u00e9lulas-tronco":[8],"hematopoi\u00e9ticas":[9],"(TCTH)":[10],"\u00e9":[11,19],"fundamental":[12],"para":[13,79,116,217,230,271],"o":[14,80,92,234,255],"monitoramento":[15],"da":[16],"enxertia.":[17],"Ela":[18],"realizada":[20,55],"por":[21,30,33,56,170],"repeti\u00e7\u00f5es":[22],"curtas":[23],"em":[24,62,101,120,275],"tandem":[25,28],"(STR,":[26],"short":[27],"repeats),":[29],"cari\u00f3tipo":[31],"e":[32,65,95,104,135,153,172,188,204,233,282],"hibrida\u00e7\u00e3o":[34],"in":[35,40],"situ":[36,41],"fluorescente":[37],"(FISH,":[38],"fluorescence":[39],"hybridization),":[42],"que":[43],"constituem":[44],"os":[45,131,199],"principais":[46],"m\u00e9todos":[47,200],"atualmente":[48,258],"empregados,":[49],"sendo":[50,279],"a":[51,72,96,123,218,261,266],"an\u00e1lise":[52,68],"FISH":[54,75,268],"leitura":[57,109,193,210],"manual.":[58],"Diante":[59],"dos":[60],"avan\u00e7os":[61],"sondas":[63],"moleculares":[64],"sistemas":[66],"automatizada,":[69],"foi":[70,277],"proposta":[71],"valida\u00e7\u00e3o":[73,176],"XY":[76,269],"com":[77,108,133,136,198,246,254],"sonda":[78],"gene":[81,93],"SRY":[82,94],"(que":[83],"analisa":[84],"as":[85],"regi\u00f5es":[86],"centr\u00f4mero":[88],"cromossomo":[90,99],"X,":[91],"heterocromatina":[97],"Y)":[100],"100,":[102],"200":[103],"300":[105],"n\u00facleos":[106,213],"interf\u00e1sicos,":[107],"automatizada.":[110],"Objetivo:":[111],"Estabelecer":[112],"valores":[113,227],"refer\u00eancia":[115,229],"detec\u00e7\u00e3o":[117,219,240,272],"pacientes":[121,156],"submetidos":[122],"TCTH":[124],"doadores":[126],"sexo":[128,249],"oposto,":[129],"correlacionando":[130],"achados":[132],"STR":[134,171],"cari\u00f3tipo.":[137,173],"M\u00e9todo:":[138],"Foram":[139,222],"analisadas":[140],"22":[141],"amostras":[142],"sedimentos":[144],"(pellets)":[145],"medula":[147],"\u00f3ssea:":[148],"10":[149],"indiv\u00edduos":[151],"controles":[152],"12":[154],"p\u00f3s-TCTH,":[157],"previamente":[158],"caracterizados":[159],"quanto":[160],"ao":[161],"(quimerismo":[163],"completo,":[164],"parcial":[165],"ou":[166],"perda":[167],"quimerismo)":[169],"Resultados:":[174],"considerou":[177],"crit\u00e9rios":[178],"como":[179],"efici\u00eancia":[180],"hibrida\u00e7\u00e3o,":[182],"intensidade":[183],"sinal,":[185],"reprodutibilidade,":[186],"especificidade":[187],"sensibilidade":[189],"anal\u00edtica":[190],"\u226598%.":[191],"automatizada":[194,270],"demonstrou":[195],"alta":[196],"concord\u00e2ncia":[197],"convencionais":[201],"(FISH":[202],"manual":[203,257],"STR),":[205],"evidenciando":[206],"robustez":[207],"anal\u00edtica.":[208],"100":[212],"interf\u00e1sicos":[214],"mostrou-se":[215],"satisfat\u00f3ria":[216],"quimerismo.":[221],"definidos":[223],"par\u00e2metros":[224],"leitura,":[226],"sinais":[231],"inespec\u00edficos":[232],"crit\u00e9rio":[235],"positividade":[237],"baseado":[238],"na":[239,284],"pelo":[242],"menos":[243],"um":[244],"n\u00facleo":[245],"sinal":[247],"oposto.":[250],"Conclus\u00e3o:":[251],"compara\u00e7\u00e3o":[253],"padr\u00e3o":[256],"utilizado":[259],"confirmou":[260],"acur\u00e1cia":[262],"protocolo.":[264],"Assim,":[265],"metodologia":[267],"interfase":[276],"validada,":[278],"considerada":[280],"segura":[281],"aplic\u00e1vel":[283],"rotina":[285],"cl\u00ednica.":[286]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2026-02-03T00:00:00"}