{"id":"https://openalex.org/W7127047384","doi":"https://doi.org/10.1016/j.psj.2026.106585","title":"Highly efficient gene editing via targeted Cas9 insertion into chicken housekeeping gene","display_name":"Highly efficient gene editing via targeted Cas9 insertion into chicken housekeeping gene","publication_year":2026,"publication_date":"2026-02-01","ids":{"openalex":"https://openalex.org/W7127047384","doi":"https://doi.org/10.1016/j.psj.2026.106585","pmid":"https://pubmed.ncbi.nlm.nih.gov/41653634"},"language":"en","primary_location":{"id":"doi:10.1016/j.psj.2026.106585","doi":"https://doi.org/10.1016/j.psj.2026.106585","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.psj.2026.106585","pdf_url":null,"source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.psj.2026.106585","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047636711","display_name":"Kyung Min Jung","orcid":"https://orcid.org/0000-0002-5791-2655","observed_orcids":["https://orcid.org/0000-0002-5791-2655"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kyung Min Jung","raw_affiliation_strings":["Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA"],"raw_orcid":"https://orcid.org/0000-0002-5791-2655","affiliations":[{"raw_affiliation_string":"Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124742665","display_name":"Rachel Klein","orcid":"https://orcid.org/0009-0009-0636-9005","observed_orcids":["https://orcid.org/0009-0009-0636-9005"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rachel Klein","raw_affiliation_strings":["Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA"],"raw_orcid":"https://orcid.org/0009-0009-0636-9005","affiliations":[{"raw_affiliation_string":"Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006083757","display_name":"Sabrina Islam Mony","orcid":"https://orcid.org/0000-0002-5363-8687","observed_orcids":["https://orcid.org/0000-0002-5363-8687"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sabrina I Mony","raw_affiliation_strings":["Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA"],"raw_orcid":"https://orcid.org/0000-0002-5363-8687","affiliations":[{"raw_affiliation_string":"Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124737887","display_name":"Paula R Chen","orcid":"https://orcid.org/0000-0003-4134-5235","observed_orcids":["https://orcid.org/0000-0003-4134-5235"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paula R Chen","raw_affiliation_strings":["USDA-ARS, Plant Genetics Research Unit, Columbia, Missouri, USA"],"raw_orcid":"https://orcid.org/0000-0003-4134-5235","affiliations":[{"raw_affiliation_string":"USDA-ARS, Plant Genetics Research Unit, Columbia, Missouri, USA","institution_ids":["https://openalex.org/I1312222531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124725336","display_name":"Kiho Lee","orcid":"https://orcid.org/0000-0003-1548-0513","observed_orcids":["https://orcid.org/0000-0003-1548-0513"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kiho Lee","raw_affiliation_strings":["Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA; National Swine Resource and Research Center, Columbia, Missouri, USA. Electronic address: kiholee@missouri.edu"],"raw_orcid":"https://orcid.org/0000-0003-1548-0513","affiliations":[{"raw_affiliation_string":"Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA; National Swine Resource and Research Center, Columbia, Missouri, USA. Electronic address: kiholee@missouri.edu","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5124704266","display_name":"Hong Jo Lee","orcid":"https://orcid.org/0009-0000-4462-0779","observed_orcids":["https://orcid.org/0009-0000-4462-0779"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hong Jo Lee","raw_affiliation_strings":["Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA. Electronic address: hongjolee@missouri.edu"],"raw_orcid":"https://orcid.org/0009-0000-4462-0779","affiliations":[{"raw_affiliation_string":"Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA. Electronic address: hongjolee@missouri.edu","institution_ids":["https://openalex.org/I76835614"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5124704266","https://openalex.org/A5124725336"],"corresponding_institution_ids":["https://openalex.org/I76835614"],"apc_list":{"value":2600,"currency":"USD","value_usd":2600},"apc_paid":{"value":2600,"currency":"USD","value_usd":2600},"fwci":1.9386,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.80028224,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"105","issue":"4","first_page":"106585","last_page":"106585"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.09449999779462814,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/housekeeping-genes","display_name":"housekeeping genes","score":0.8650000095367432},{"id":"https://openalex.org/keywords/beta-actin","display_name":"beta-actin","score":0.9309999942779541},{"id":"https://openalex.org/keywords/glyceraldehyde-3-phosphate-dehydrogenase","display_name":"glyceraldehyde-3-phosphate dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/transgene-stability","display_name":"transgene stability","score":0.6629999876022339},{"id":"https://openalex.org/keywords/epigenetic-silencing","display_name":"epigenetic silencing","score":0.7839999794960022},{"id":"https://openalex.org/keywords/site-specific-integration","display_name":"site-specific integration","score":0.5979999899864197},{"id":"https://openalex.org/keywords/genetic-tagging","display_name":"gene tagging","score":0.7540000081062317},{"id":"https://openalex.org/keywords/gfp-expression","display_name":"GFP expression","score":0.5059999823570251},{"id":"https://openalex.org/keywords/single-cell-clones","display_name":"single-cell clones","score":0.7229999899864197},{"id":"https://openalex.org/keywords/df-1-cells","display_name":"DF-1 cells","score":0.8090000152587891}],"concepts":[{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.8029000163078308},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8021000027656555},{"id":"https://openalex.org/C73247094","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Transgenesis","level":5,"score":0.7986000180244446},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7980999946594238},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7598000168800354},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.6589000225067139},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.647599995136261},{"id":"https://openalex.org/C33922130","wikidata":"https://www.wikidata.org/wiki/Q419232","display_name":"Housekeeping gene","level":4,"score":0.5595999956130981},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5587000250816345},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5514000058174133},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.512499988079071},{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.428600013256073},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.3781000077724457},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.37299999594688416},{"id":"https://openalex.org/C161078062","wikidata":"https://www.wikidata.org/wiki/Q1068690","display_name":"Functional genomics","level":5,"score":0.3666999936103821},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.36469998955726624},{"id":"https://openalex.org/C111829913","wikidata":"https://www.wikidata.org/wiki/Q211083","display_name":"Gene targeting","level":3,"score":0.3549000024795532},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.3334999978542328},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.33149999380111694},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.2702000141143799},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.26759999990463257},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.25949999690055847},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.25110000371932983}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019076","descriptor_name":"Transgenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020043","descriptor_name":"Genes, Essential","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D030161","descriptor_name":"Avian Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1016/j.psj.2026.106585","doi":"https://doi.org/10.1016/j.psj.2026.106585","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.psj.2026.106585","pdf_url":null,"source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},{"id":"pmid:41653634","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41653634","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry science","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:11712817","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12906158","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:4c816c6cb5004797945d07d62401343d","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/4c816c6cb5004797945d07d62401343d","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Poultry Science, Vol 105, Iss 4, Pp 106585- (2026)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12906158","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12906158/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Poult Sci","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1016/j.psj.2026.106585","doi":"https://doi.org/10.1016/j.psj.2026.106585","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.psj.2026.106585","pdf_url":null,"source":{"id":"https://openalex.org/S133896805","display_name":"Poultry Science","issn_l":"0032-5791","issn":["0032-5791","1525-3171"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Poultry Science","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.40450000762939453}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5191893503","display_name":null,"funder_award_id":"2025-67015-44824","funder_id":"https://openalex.org/F4320332299","funder_display_name":"National Institute of Food and Agriculture"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320332299","display_name":"National Institute of Food and Agriculture","ror":"https://ror.org/05qx3fv49"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W644569968","https://openalex.org/W1768274470","https://openalex.org/W1992925805","https://openalex.org/W2045435533","https://openalex.org/W2086401718","https://openalex.org/W2094979867","https://openalex.org/W2108157524","https://openalex.org/W2123207873","https://openalex.org/W2192551925","https://openalex.org/W2461013690","https://openalex.org/W2606856801","https://openalex.org/W2752910994","https://openalex.org/W2789218522","https://openalex.org/W2897141255","https://openalex.org/W2970696909","https://openalex.org/W3032288647","https://openalex.org/W3088575657","https://openalex.org/W3097267181","https://openalex.org/W3131779049","https://openalex.org/W3158436096","https://openalex.org/W3216716366","https://openalex.org/W4224140475","https://openalex.org/W4281714389","https://openalex.org/W4312195490","https://openalex.org/W4365448641","https://openalex.org/W4381930778","https://openalex.org/W4412025740","https://openalex.org/W4412706599"],"related_works":[],"abstract_inverted_index":{"Achieving":[0],"stable":[1,72,115,147],"and":[2,18,38,52,73,105,116,127,133,154,179,205,219],"efficient":[3,109,162],"transgene":[4,75,118,214],"expression":[5,41,54,76,148,215],"is":[6],"a":[7,209],"key":[8],"challenge":[9],"in":[10,25,89,112,140,151,183,216],"advancing":[11],"avian":[12],"genome":[13,181,220],"engineering.":[14,221],"Although":[15],"viral":[16],"vector-based":[17],"piggyBac-mediated":[19],"transgenesis":[20],"have":[21],"been":[22,87],"widely":[23],"used":[24,47],"chickens,":[26],"both":[27],"approaches":[28,145],"are":[29],"prone":[30],"to":[31,35],"epigenetic":[32],"silencing,":[33,207],"leading":[34],"inconsistent,":[36],"tissue-specific,":[37],"often":[39],"diminished":[40],"over":[42],"time.":[43],"This":[44],"variability":[45],"limits":[46],"of":[48,149,188,202],"transgenes":[49,150,189],"requiring":[50],"robust":[51,210],"long-term":[53],"across":[55],"multiple":[56],"tissues.":[57],"In":[58,91],"mammals,":[59],"site-specific":[60],"integration":[61,204],"into":[62,99,136,190],"genomic":[63,138],"safe":[64,196],"harbor":[65,197],"loci,":[66],"such":[67,83],"as":[68,194],"Rosa26,":[69],"has":[70,85],"enabled":[71],"predictable":[74],"without":[77],"disrupting":[78],"endogenous":[79,100],"gene":[80,110,125,128],"function;":[81],"however,":[82],"strategy":[84],"not":[86],"established":[88],"birds.":[90],"this":[92],"research,":[93],"we":[94,130,170],"hypothesized":[95],"that":[96,158],"integrating":[97],"Cas9":[98,132,159],"housekeeping":[101,192],"genes":[102,193],"(the":[103],"ACTB":[104],"GAPDH)":[106],"could":[107],"achieve":[108],"editing":[111,182],"chickens":[113],"through":[114],"ubiquitous":[117],"expression.":[119],"Using":[120],"two":[121],"different":[122],"approaches,":[123],"3'-targeted":[124],"insertion":[126,187],"tagging,":[129],"inserted":[131],"GFP":[134],"cassettes":[135],"defined":[137],"loci":[139,198],"chicken":[141],"DF-1":[142],"cells.":[143],"Both":[144],"exhibited":[146],"the":[152,200],"cells,":[153],"functional":[155],"assays":[156],"confirmed":[157],"showed":[160],"highly":[161],"nuclease":[163],"activity":[164],"following":[165],"guide":[166],"RNA":[167],"delivery.":[168],"Additionally,":[169],"derived":[171],"single-cell":[172],"clones":[173],"stably":[174],"expressing":[175],"Cas9,":[176],"enabling":[177],"uniform":[178],"reproducible":[180],"downstream":[184],"applications.":[185],"Targeted":[186],"active":[191],"candidate":[195],"mitigates":[199],"limitations":[201],"random":[203],"promoter":[206],"offering":[208],"platform":[211],"for":[212],"consistent":[213],"poultry":[217],"biotechnology":[218]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-09-25T08:18:29.962427","created_date":"2026-02-03T00:00:00"}