{"id":"https://openalex.org/W7126090696","doi":"https://doi.org/10.1002/ijch.70009","title":"A Regioselective and Modular Chemoenzymatic Strategy for the Synthesis of O \u2010GalNAc Glycans and Disialyl Galacto\u2010 N \u2010Bioses","display_name":"A Regioselective and Modular Chemoenzymatic Strategy for the Synthesis of O \u2010GalNAc Glycans and Disialyl Galacto\u2010 N \u2010Bioses","publication_year":2026,"publication_date":"2026-01-01","ids":{"openalex":"https://openalex.org/W7126090696","doi":"https://doi.org/10.1002/ijch.70009"},"language":"en","primary_location":{"id":"doi:10.1002/ijch.70009","doi":"https://doi.org/10.1002/ijch.70009","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ijch.70009","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijch.70009","source":{"id":"https://openalex.org/S195061198","display_name":"Israel Journal of Chemistry","issn_l":"0021-2148","issn":["0021-2148","1869-5868"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Israel Journal of Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijch.70009","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020168253","display_name":"Min-Cheng Chien","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Min\u2010Cheng Chien","raw_affiliation_strings":["Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5124210724","display_name":"Ting\u2010Yi Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Ting\u2010Yi Lee","raw_affiliation_strings":["Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067406909","display_name":"Avijit Kumar Adak","orcid":"https://orcid.org/0000-0002-5907-3744","observed_orcids":["https://orcid.org/0000-0002-5907-3744"]},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Avijit K. Adak","raw_affiliation_strings":["Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5124285414","display_name":"Chun\u2010Cheng Lin","orcid":"https://orcid.org/0000-0002-2323-0920","observed_orcids":["https://orcid.org/0000-0002-2323-0920"]},"institutions":[{"id":"https://openalex.org/I152743029","display_name":"Kaohsiung Medical University","ror":"https://ror.org/03gk81f96","country_code":"TW","type":"education","lineage":["https://openalex.org/I152743029"]},{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]}],"countries":["TW"],"is_corresponding":true,"raw_author_name":"Chun\u2010Cheng Lin","raw_affiliation_strings":["Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan","Department of Medicinal and Applied Chemistry Kaohsiung Medical University  Kaohsiung 807378 Taiwan"],"raw_orcid":"https://orcid.org/0000-0002-2323-0920","affiliations":[{"raw_affiliation_string":"Department of Chemistry National Tsing Hua University  101 Section 2, Kuang Fu Road Hsinchu 300044 Taiwan","institution_ids":["https://openalex.org/I25846049"]},{"raw_affiliation_string":"Department of Medicinal and Applied Chemistry Kaohsiung Medical University  Kaohsiung 807378 Taiwan","institution_ids":["https://openalex.org/I152743029"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5124285414"],"corresponding_institution_ids":["https://openalex.org/I152743029","https://openalex.org/I25846049"],"apc_list":{"value":4650,"currency":"USD","value_usd":4650},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"66","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.9101999998092651,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.9101999998092651,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.08739999681711197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chemoenzymatic-synthesis","display_name":"chemoenzymatic synthesis","score":0.8259999752044678},{"id":"https://openalex.org/keywords/mucin-type-o-glycans","display_name":"mucin-type O-glycans","score":0.8849999904632568},{"id":"https://openalex.org/keywords/regioselective-glycosylation","display_name":"regioselective glycosylation","score":0.8119999766349792},{"id":"https://openalex.org/keywords/alpha2-3-sialylation","display_name":"\u03b12,3-sialylation","score":0.9449999928474426},{"id":"https://openalex.org/keywords/alpha2-6-sialylation","display_name":"\u03b12,6-sialylation","score":0.9980000257492065},{"id":"https://openalex.org/keywords/glycosyltransferases","display_name":"glycosyltransferases","score":0.7369999885559082},{"id":"https://openalex.org/keywords/glycan-synthesis","display_name":"glycan synthesis","score":0.3799999952316284}],"concepts":[{"id":"https://openalex.org/C59759590","wikidata":"https://www.wikidata.org/wiki/Q2275927","display_name":"Regioselectivity","level":3,"score":0.9273999929428101},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9253000020980835},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.6568999886512756},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.6141999959945679},{"id":"https://openalex.org/C7947691","wikidata":"https://www.wikidata.org/wiki/Q567575","display_name":"Anomer","level":2,"score":0.5669999718666077},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.5552999973297119},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5038999915122986},{"id":"https://openalex.org/C131779359","wikidata":"https://www.wikidata.org/wiki/Q471875","display_name":"Amine gas treating","level":2,"score":0.4602000117301941},{"id":"https://openalex.org/C161019410","wikidata":"https://www.wikidata.org/wiki/Q67201373","display_name":"Glycosyltransferase","level":3,"score":0.44429999589920044},{"id":"https://openalex.org/C101468663","wikidata":"https://www.wikidata.org/wiki/Q1620158","display_name":"Modular design","level":2,"score":0.44119998812675476},{"id":"https://openalex.org/C191503008","wikidata":"https://www.wikidata.org/wiki/Q16007026","display_name":"Biocatalysis","level":4,"score":0.43459999561309814},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.42250001430511475},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.414900004863739},{"id":"https://openalex.org/C41685203","wikidata":"https://www.wikidata.org/wiki/Q1974042","display_name":"Docking (animal)","level":2,"score":0.4083000123500824},{"id":"https://openalex.org/C129694887","wikidata":"https://www.wikidata.org/wiki/Q47455153","display_name":"Stereoisomerism","level":3,"score":0.35179999470710754},{"id":"https://openalex.org/C53105671","wikidata":"https://www.wikidata.org/wiki/Q14777816","display_name":"Rational design","level":2,"score":0.3492000102996826},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.3206999897956848},{"id":"https://openalex.org/C170640332","wikidata":"https://www.wikidata.org/wiki/Q5037882","display_name":"Carbohydrate synthesis","level":3,"score":0.30869999527931213},{"id":"https://openalex.org/C2779892579","wikidata":"https://www.wikidata.org/wiki/Q912138","display_name":"Acceptor","level":2,"score":0.3000999987125397},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.26660001277923584}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1002/ijch.70009","doi":"https://doi.org/10.1002/ijch.70009","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ijch.70009","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijch.70009","source":{"id":"https://openalex.org/S195061198","display_name":"Israel Journal of Chemistry","issn_l":"0021-2148","issn":["0021-2148","1869-5868"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Israel Journal of Chemistry","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1002/ijch.70009","doi":"https://doi.org/10.1002/ijch.70009","is_oa":true,"landing_page_url":"https://doi.org/10.1002/ijch.70009","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijch.70009","source":{"id":"https://openalex.org/S195061198","display_name":"Israel Journal of Chemistry","issn_l":"0021-2148","issn":["0021-2148","1869-5868"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Israel Journal of Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3105685435","display_name":null,"funder_award_id":"113\u20102113\u2010M\u2010007\u2010024\u2010MY3 and 113\u20102113\u2010M\u2010007\u2010004","funder_id":"https://openalex.org/F2461203286","funder_display_name":"National Science and Technology Council"},{"id":"https://openalex.org/G5888802319","display_name":null,"funder_award_id":"AS-GC-111-M03","funder_id":"https://openalex.org/F4320321041","funder_display_name":"Academia Sinica"}],"funders":[{"id":"https://openalex.org/F2461203286","display_name":"National Science and Technology Council","ror":"https://ror.org/02kv4zf79"},{"id":"https://openalex.org/F4320321041","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784"},{"id":"https://openalex.org/F4320323092","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7126090696.pdf","grobid_xml":"https://content.openalex.org/works/W7126090696.grobid-xml"},"referenced_works_count":43,"referenced_works":["https://openalex.org/W53883719","https://openalex.org/W197316820","https://openalex.org/W1182461782","https://openalex.org/W1505123947","https://openalex.org/W1945947879","https://openalex.org/W1967511968","https://openalex.org/W1975933816","https://openalex.org/W1977503027","https://openalex.org/W1978543241","https://openalex.org/W2003885329","https://openalex.org/W2007163769","https://openalex.org/W2020620720","https://openalex.org/W2024191848","https://openalex.org/W2028752174","https://openalex.org/W2028854304","https://openalex.org/W2046956659","https://openalex.org/W2054426881","https://openalex.org/W2095535715","https://openalex.org/W2104294360","https://openalex.org/W2124170577","https://openalex.org/W2134967712","https://openalex.org/W2153604498","https://openalex.org/W2156560231","https://openalex.org/W2314270777","https://openalex.org/W2761663881","https://openalex.org/W2807830322","https://openalex.org/W2871871856","https://openalex.org/W2921437380","https://openalex.org/W2947112244","https://openalex.org/W3094407181","https://openalex.org/W3139268557","https://openalex.org/W3170932197","https://openalex.org/W3216412188","https://openalex.org/W4319996040","https://openalex.org/W4390097302","https://openalex.org/W4391162790","https://openalex.org/W4393621654","https://openalex.org/W4399566633","https://openalex.org/W4403483942","https://openalex.org/W4404474223","https://openalex.org/W4408813740","https://openalex.org/W4414167568","https://openalex.org/W4415166206"],"related_works":["https://openalex.org/W1992084241","https://openalex.org/W1945947879","https://openalex.org/W3035876257","https://openalex.org/W2613130018","https://openalex.org/W3035709633","https://openalex.org/W2151948581","https://openalex.org/W2016392690","https://openalex.org/W2462094902","https://openalex.org/W3178619793","https://openalex.org/W2077020373"],"abstract_inverted_index":{"Glycosylation":[0],"plays":[1],"a":[2,149,206],"vital":[3],"role":[4],"in":[5,68,115,170,187,202,219],"cellular":[6],"functions,":[7],"yet":[8],"the":[9,49,142,177,188,197],"synthesis":[10,51,198],"of":[11,52,59,199],"complex":[12],"mucin\u2010type":[13],"O":[14,135,144,155,172,190,213],"\u2010glycans":[15],"such":[16],"as":[17],"disialyl":[18,200],"galacto\u2010":[19],"N":[20,94],"\u2010biose":[21],"(DSGNB)":[22],"remains":[23],"significant":[24],"challenge":[25],"due":[26],"to":[27,79,161,183],"structural":[28],"complexity":[29],"and":[30,43,72,93,105,131,222],"enzyme":[31],"specificity.":[32],"A":[33,57],"modular":[34],"chemoenzymatic":[35],"strategy":[36],"employing":[37],"bacterial":[38],"glycosyltransferases":[39],"BiGalHexNAcP,":[40],"LgtD,":[41],"Cst\u2010I,":[42],"an":[44],"engineered":[45],"Psp2,6ST":[46,124],"A366G":[47],"for":[48,88,210],"regioselective":[50],"DSGNB":[53,126],"analogs":[54],"is":[55,180],"reported.":[56],"panel":[58],"GalNR\u2010based":[60],"acceptors":[61],"(":[62,127],"11":[63],"\u2013":[64,112],"14":[65],"),":[66],"varying":[67],"anomeric":[69],"configuration,":[70],"aglycone,":[71],"amine":[73],"protection,":[74],"enables":[75,196],"sequential":[76],"one\u2010pot":[77],"\u03b21,3\u2010galactosylation":[78],"generate":[80],"diverse":[81],"GNB":[82],"derivatives.":[83],"BiGalHexNAcP":[84],"exhibits":[85],"strong":[86],"preference":[87],"\u03b1\u2010GalNR":[89],"with":[90,103,123,216],"allyl":[91],"linkers":[92],"\u2010acetyl\u2010protection,":[95],"whereas":[96],"LgtD":[97],"shows":[98],"broader":[99],"tolerance.":[100],"Subsequent":[101],"\u03b12,3\u2010sialylation":[102],"Cst\u2010I":[104],"CMP\u2010Neu5Ac":[106],"affords":[107],"monosialyl":[108],"GNBs":[109],"(MSGNB,":[110],"2":[111,147],"10":[113],")":[114,129],"80%\u2013100%":[116],"yield":[117],"within":[118],"1\u20132":[119],"h.":[120],"Final":[121],"\u03b12,6\u2010sialylation":[122],"furnishes":[125],"1b":[128],"regio\u2010":[130],"stereoselectively":[132],"from":[133],"\u03b2\u2010":[134,154,171],"\u2010allyl":[136,145,173],"MSGNB":[137,174],"3":[138,175],"(93%":[139],"yield),":[140],"while":[141],"\u03b1\u2010":[143,189],"acceptor":[146],"produces":[148],"DSGNB:iso\u2010DSGNB":[150],"mixture":[151],"(2:1).":[152],"Notably,":[153],"\u2010benzyl":[156],"aglycones":[157],"further":[158],"enhance":[159],"regioselectivity":[160],"6:1.":[162],"Molecular":[163],"docking":[164],"provides":[165],"mechanistic":[166],"insight,":[167],"showing":[168],"that":[169],",":[176],"GalNAc":[178],"C6\u2010OH":[179],"positioned":[181],"closer":[182],"catalytic":[184],"Asp232":[185],"than":[186],"\u2010linked":[191],"analog.":[192],"The":[193],"method":[194],"also":[195],"T\u2010antigen":[201],"70%":[203],"yield,":[204],"demonstrating":[205],"broadly":[207],"applicable":[208],"platform":[209],"constructing":[211],"well\u2010defined":[212],"\u2010GalNAc":[214],"glycans":[215],"potential":[217],"applications":[218],"biomedical":[220],"research":[221],"therapeutic":[223],"development.":[224]},"counts_by_year":[],"updated_date":"2026-09-25T08:18:29.962427","created_date":"2026-01-30T00:00:00"}