{"id":"https://openalex.org/W7125521115","doi":"https://doi.org/10.55251/jmbfs.13541","title":"HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): PRINCIPLE, APPLICATIONS, AND THE ROLE OF ARTIFICIAL INTELLIGENCE \u2013 A REVIEW","display_name":"HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): PRINCIPLE, APPLICATIONS, AND THE ROLE OF ARTIFICIAL INTELLIGENCE \u2013 A REVIEW","publication_year":2025,"publication_date":"2025-12-05","ids":{"openalex":"https://openalex.org/W7125521115","doi":"https://doi.org/10.55251/jmbfs.13541"},"language":"en","primary_location":{"id":"doi:10.55251/jmbfs.13541","doi":"https://doi.org/10.55251/jmbfs.13541","is_oa":true,"landing_page_url":"https://doi.org/10.55251/jmbfs.13541","pdf_url":"https://office2.jmbfs.org/index.php/JMBFS/article/download/13541/3849","source":{"id":"https://openalex.org/S2764909764","display_name":"Journal of Microbiology Biotechnology and Food Sciences","issn_l":"1338-5178","issn":["1338-5178"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310314450","host_organization_name":"Slovak University of Agriculture","host_organization_lineage":["https://openalex.org/P4310314450"],"host_organization_lineage_names":["Slovak University of Agriculture"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology, biotechnology and food sciences","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://office2.jmbfs.org/index.php/JMBFS/article/download/13541/3849","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109643783","display_name":"Fakeha Mohammed Rehan Shaikh","orcid":"https://orcid.org/0009-0004-4485-8989","observed_orcids":["https://orcid.org/0009-0004-4485-8989"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Fakeha Shaikh","raw_affiliation_strings":["Department of chemistry, Wilson College ( Autonomous), Mumbai-400007"],"raw_orcid":"https://orcid.org/0009-0004-4485-8989","affiliations":[{"raw_affiliation_string":"Department of chemistry, Wilson College ( Autonomous), Mumbai-400007","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5115984814","display_name":"Ashish Sambhaji Uzgare","orcid":"https://orcid.org/0000-0002-0357-9770","observed_orcids":["https://orcid.org/0000-0002-0357-9770"]},"institutions":[{"id":"https://openalex.org/I50219821","display_name":"Wilson College","ror":"https://ror.org/05japer20","country_code":"US","type":"education","lineage":["https://openalex.org/I50219821"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ashish Uzgare","raw_affiliation_strings":["John Wilson Education Society's Wilson College Autonomous"],"raw_orcid":"https://orcid.org/0000-0002-0357-9770","affiliations":[{"raw_affiliation_string":"John Wilson Education Society's Wilson College Autonomous","institution_ids":["https://openalex.org/I50219821"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4432,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.39302326,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":null,"issue":null,"first_page":"e13541","last_page":"e13541"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-performance-liquid-chromatography","display_name":"HPLC","score":0.9959999918937683},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"artificial intelligence","score":0.656000018119812},{"id":"https://openalex.org/keywords/chromatographic-separation","display_name":"chromatographic separation","score":0.6200000047683716},{"id":"https://openalex.org/keywords/drug-quantification","display_name":"pharmaceutical analysis","score":0.7409999966621399},{"id":"https://openalex.org/keywords/food-safety","display_name":"food safety","score":0.7310000061988831},{"id":"https://openalex.org/keywords/contaminant-detection","display_name":"contamination detection","score":0.8849999904632568},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":1.0},{"id":"https://openalex.org/keywords/environmental-monitoring","display_name":"environmental monitoring","score":0.8169999718666077},{"id":"https://openalex.org/keywords/column-design","display_name":"column design","score":0.8980000019073486},{"id":"https://openalex.org/keywords/detection-technology","display_name":"detection technology","score":0.8460000157356262}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4812000095844269},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.4609000086784363},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.4440999925136566},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.42160001397132874},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.41100001335144043},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.30300000309944153},{"id":"https://openalex.org/C3020199158","wikidata":"https://www.wikidata.org/wiki/Q210521","display_name":"High resolution","level":2,"score":0.2962999939918518},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2791999876499176},{"id":"https://openalex.org/C2780551164","wikidata":"https://www.wikidata.org/wiki/Q2306599","display_name":"Column (typography)","level":3,"score":0.2775000035762787},{"id":"https://openalex.org/C157170001","wikidata":"https://www.wikidata.org/wiki/Q4781507","display_name":"Applications of artificial intelligence","level":2,"score":0.2736999988555908},{"id":"https://openalex.org/C12713177","wikidata":"https://www.wikidata.org/wiki/Q1900281","display_name":"Perspective (graphical)","level":2,"score":0.2669000029563904}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.55251/jmbfs.13541","doi":"https://doi.org/10.55251/jmbfs.13541","is_oa":true,"landing_page_url":"https://doi.org/10.55251/jmbfs.13541","pdf_url":"https://office2.jmbfs.org/index.php/JMBFS/article/download/13541/3849","source":{"id":"https://openalex.org/S2764909764","display_name":"Journal of Microbiology Biotechnology and Food Sciences","issn_l":"1338-5178","issn":["1338-5178"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310314450","host_organization_name":"Slovak University of Agriculture","host_organization_lineage":["https://openalex.org/P4310314450"],"host_organization_lineage_names":["Slovak University of Agriculture"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology, biotechnology and food sciences","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.55251/jmbfs.13541","doi":"https://doi.org/10.55251/jmbfs.13541","is_oa":true,"landing_page_url":"https://doi.org/10.55251/jmbfs.13541","pdf_url":"https://office2.jmbfs.org/index.php/JMBFS/article/download/13541/3849","source":{"id":"https://openalex.org/S2764909764","display_name":"Journal of Microbiology Biotechnology and Food Sciences","issn_l":"1338-5178","issn":["1338-5178"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310314450","host_organization_name":"Slovak University of Agriculture","host_organization_lineage":["https://openalex.org/P4310314450"],"host_organization_lineage_names":["Slovak University of Agriculture"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microbiology, biotechnology and food sciences","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7125521115.pdf","grobid_xml":"https://content.openalex.org/works/W7125521115.grobid-xml"},"referenced_works_count":45,"referenced_works":["https://openalex.org/W123445147","https://openalex.org/W1557721692","https://openalex.org/W1972330837","https://openalex.org/W1994016219","https://openalex.org/W2024051169","https://openalex.org/W2030207943","https://openalex.org/W2051351267","https://openalex.org/W2076393768","https://openalex.org/W2091070913","https://openalex.org/W2142498673","https://openalex.org/W2280882319","https://openalex.org/W2407464177","https://openalex.org/W2937728757","https://openalex.org/W2939432126","https://openalex.org/W2942648928","https://openalex.org/W3015145991","https://openalex.org/W3082945565","https://openalex.org/W3096580416","https://openalex.org/W3126912009","https://openalex.org/W3181861100","https://openalex.org/W3200568586","https://openalex.org/W3208951284","https://openalex.org/W4200149805","https://openalex.org/W4252140821","https://openalex.org/W4285174688","https://openalex.org/W4299851340","https://openalex.org/W4365810941","https://openalex.org/W4376573478","https://openalex.org/W4385067258","https://openalex.org/W4394683464","https://openalex.org/W4396797699","https://openalex.org/W4399390211","https://openalex.org/W4400357457","https://openalex.org/W4402205848","https://openalex.org/W4404797887","https://openalex.org/W4405140066","https://openalex.org/W4406016148","https://openalex.org/W4406216652","https://openalex.org/W4407181428","https://openalex.org/W4407242280","https://openalex.org/W4407260244","https://openalex.org/W4408456935","https://openalex.org/W4410193996","https://openalex.org/W4412413308","https://openalex.org/W4413933418"],"related_works":["https://openalex.org/W7169843449","https://openalex.org/W4403479594","https://openalex.org/W7115719846","https://openalex.org/W4409589397","https://openalex.org/W6892730634","https://openalex.org/W4402205848","https://openalex.org/W7130516075","https://openalex.org/W4400540346","https://openalex.org/W7167599079","https://openalex.org/W4385979328"],"abstract_inverted_index":{"High-Performance":[0],"Liquid":[1],"Chromatography":[2],"(HPLC)":[3],"is":[4],"a":[5,58,138],"versatile":[6],"and":[7,16,25,45,90,114,136,147],"powerful":[8],"analytical":[9,127],"technique":[10],"widely":[11],"employed":[12],"to":[13],"separate,":[14],"detect,":[15],"quantify":[17],"components":[18],"of":[19,32,41,51,61,121],"complex":[20],"mixtures":[21],"with":[22,43],"high":[23],"precision":[24],"accuracy.":[26],"Rooted":[27],"in":[28,77,82,91,109,145],"the":[29,38,119],"fundamental":[30,66],"principles":[31],"chromatography,":[33],"HPLC":[34,105],"operates":[35],"by":[36,132],"exploiting":[37],"differential":[39],"interactions":[40],"analytes":[42],"stationary":[44],"mobile":[46],"phases,":[47],"enabling":[48],"efficient":[49],"resolution":[50],"diverse":[52],"chemical":[53],"compounds.":[54],"This":[55],"review":[56,130],"presents":[57],"comprehensive":[59],"overview":[60],"HPLC,":[62],"elaborating":[63],"on":[64,141],"its":[65],"principles,":[67],"also":[68],"emphasizes":[69],"HPLC\u2019s":[70,142],"extensive":[71],"applications":[72],"across":[73],"multiple":[74],"fields,":[75],"particularly":[76],"pharmaceuticals":[78],"for":[79,85,94,125],"drug":[80],"analysis,":[81],"food":[83],"safety":[84],"contaminant":[86],"detection,":[87],"Protein":[88],"purification":[89],"environmental":[92],"monitoring":[93],"pollutant":[95],"assessment.":[96],"Additionally,":[97],"it":[98],"discusses":[99],"recent":[100],"advancements":[101],"that":[102],"have":[103],"enhanced":[104],"performance,":[106],"including":[107],"innovations":[108],"column":[110],"design,":[111],"detection":[112],"technologies,":[113],"automation,":[115],"as":[116,118],"well":[117],"integration":[120],"Artificial":[122],"intelligence":[123],"(AI)":[124],"improved":[126],"efficiency.":[128],"The":[129],"concludes":[131],"highlighting":[133],"current":[134],"limitations":[135],"offering":[137],"holistic":[139],"perspective":[140],"continued":[143],"significance":[144],"research":[146],"industrial":[148],"applications.":[149]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2026-01-24T00:00:00"}