{"id":"https://openalex.org/W7124456188","doi":"https://doi.org/10.1016/j.bbrc.2026.153300","title":"smFASTIA: A high-sensitivity platform for purification-free kinetic screening of protein\u2013small molecule interactions","display_name":"smFASTIA: A high-sensitivity platform for purification-free kinetic screening of protein\u2013small molecule interactions","publication_year":2026,"publication_date":"2026-01-16","ids":{"openalex":"https://openalex.org/W7124456188","doi":"https://doi.org/10.1016/j.bbrc.2026.153300","pmid":"https://pubmed.ncbi.nlm.nih.gov/41558199"},"language":"en","primary_location":{"id":"doi:10.1016/j.bbrc.2026.153300","doi":"https://doi.org/10.1016/j.bbrc.2026.153300","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrc.2026.153300","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/j.bbrc.2026.153300","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5123232398","display_name":"Yuki Tokunaga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuki Tokunaga","raw_affiliation_strings":["Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019278346","display_name":"Ryo Matsunaga","orcid":"https://orcid.org/0000-0001-7702-9176","observed_orcids":["https://orcid.org/0000-0001-7702-9176"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Ryo Matsunaga","raw_affiliation_strings":["Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Electronic address: ryo-matsunaga@g.ecc.u-tokyo.ac.jp"],"raw_orcid":"https://orcid.org/0000-0001-7702-9176","affiliations":[{"raw_affiliation_string":"Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. Electronic address: ryo-matsunaga@g.ecc.u-tokyo.ac.jp","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032712562","display_name":"Satoru Nagatoishi","orcid":"https://orcid.org/0000-0002-0794-3963","observed_orcids":["https://orcid.org/0000-0002-0794-3963"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Satoru Nagatoishi","raw_affiliation_strings":["Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan"],"raw_orcid":"https://orcid.org/0000-0002-0794-3963","affiliations":[{"raw_affiliation_string":"Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072979871","display_name":"Kouhei Tsumoto","orcid":"https://orcid.org/0000-0001-7643-5164","observed_orcids":["https://orcid.org/0000-0001-7643-5164"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Kouhei Tsumoto","raw_affiliation_strings":["Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp"],"raw_orcid":"https://orcid.org/0000-0001-7643-5164","affiliations":[{"raw_affiliation_string":"Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Medical Device Development and Regulation Research Center, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp","institution_ids":["https://openalex.org/I74801974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5019278346","https://openalex.org/A5072979871"],"corresponding_institution_ids":["https://openalex.org/I74801974"],"apc_list":{"value":3110,"currency":"USD","value_usd":3110},"apc_paid":{"value":3110,"currency":"USD","value_usd":3110},"fwci":1.9386,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.7904974,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"801","issue":null,"first_page":"153300","last_page":"153300"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.5648999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.5648999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.2808000147342682,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.01549999974668026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-small-molecule-interactions","display_name":"protein-small molecule interactions","score":0.8140000104904175},{"id":"https://openalex.org/keywords/binding-kinetics","display_name":"binding kinetics","score":0.9390000104904175},{"id":"https://openalex.org/keywords/structure-kinetics-relationship","display_name":"structure-kinetics relationship","score":0.7889999747276306},{"id":"https://openalex.org/keywords/cell-free-protein-synthesis","display_name":"cell-free protein synthesis","score":0.9169999957084656},{"id":"https://openalex.org/keywords/biolayer-interferometry","display_name":"biolayer interferometry","score":0.9919999837875366},{"id":"https://openalex.org/keywords/carbonic-anhydrase-ii","display_name":"carbonic anhydrase II","score":0.9290000200271606},{"id":"https://openalex.org/keywords/enzyme-inhibitors","display_name":"enzyme inhibitors","score":0.49300000071525574},{"id":"https://openalex.org/keywords/association-rate-constant","display_name":"association rate constant","score":0.8209999799728394},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C106847996","wikidata":"https://www.wikidata.org/wiki/Q898756","display_name":"Surface plasmon resonance","level":3,"score":0.5605000257492065},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5467000007629395},{"id":"https://openalex.org/C135889238","wikidata":"https://www.wikidata.org/wiki/Q46276","display_name":"Kinetic energy","level":2,"score":0.5315999984741211},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.48559999465942383},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.4487999975681305},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.4456999897956848},{"id":"https://openalex.org/C161624437","wikidata":"https://www.wikidata.org/wiki/Q1988322","display_name":"Small molecule","level":2,"score":0.43369999527931213},{"id":"https://openalex.org/C2775914622","wikidata":"https://www.wikidata.org/wiki/Q284089","display_name":"Carbonic anhydrase","level":3,"score":0.4052000045776367},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.4032000005245209},{"id":"https://openalex.org/C2780416268","wikidata":"https://www.wikidata.org/wiki/Q18021049","display_name":"Carbonic anhydrase II","level":4,"score":0.4009999930858612},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.37220001220703125},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3668999969959259},{"id":"https://openalex.org/C2780283098","wikidata":"https://www.wikidata.org/wiki/Q4688960","display_name":"Affinities","level":2,"score":0.35429999232292175},{"id":"https://openalex.org/C152900378","wikidata":"https://www.wikidata.org/wiki/Q7302366","display_name":"Receptor\u2013ligand kinetics","level":3,"score":0.3319000005722046},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3118000030517578},{"id":"https://openalex.org/C103697762","wikidata":"https://www.wikidata.org/wiki/Q4112105","display_name":"Virtual screening","level":3,"score":0.3021000027656555},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2890999913215637},{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.28780001401901245},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.28130000829696655},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.2712000012397766},{"id":"https://openalex.org/C2989108626","wikidata":"https://www.wikidata.org/wiki/Q904407","display_name":"Drug target","level":2,"score":0.26919999718666077},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.2678999900817871},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.26669999957084656},{"id":"https://openalex.org/C106131492","wikidata":"https://www.wikidata.org/wiki/Q3072260","display_name":"Filter (signal processing)","level":2,"score":0.25540000200271606}],"mesh":[{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002257","descriptor_name":"Carbonic Anhydrase Inhibitors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007368","descriptor_name":"Interferometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020349","descriptor_name":"Surface Plasmon Resonance","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D024402","descriptor_name":"Carbonic Anhydrase II","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D054852","descriptor_name":"Small Molecule Libraries","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.bbrc.2026.153300","doi":"https://doi.org/10.1016/j.bbrc.2026.153300","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrc.2026.153300","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},{"id":"pmid:41558199","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41558199","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and biophysical research communications","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.bbrc.2026.153300","doi":"https://doi.org/10.1016/j.bbrc.2026.153300","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.bbrc.2026.153300","pdf_url":null,"source":{"id":"https://openalex.org/S151589183","display_name":"Biochemical and Biophysical Research Communications","issn_l":"0006-291X","issn":["0006-291X","1090-2104"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical and Biophysical Research Communications","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.7185670137405396,"display_name":"Reduced inequalities","id":"https://metadata.un.org/sdg/10"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2582772576","display_name":"Full-body electronic skins for digital me","funder_award_id":"22K21343","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G5297492720","display_name":null,"funder_award_id":"JP24ama121033","funder_id":"https://openalex.org/F4320311405","funder_display_name":"Japan Agency for Medical Research and Development"},{"id":"https://openalex.org/G8962687670","display_name":"\u7279\u7570\u7684\u5206\u5b50\u8a8d\u8b58\u5834\u306e\u30c7\u30fc\u30bf\u99c6\u52d5\u578b\u8a2d\u8a08","funder_award_id":"JPMJAX222I","funder_id":"https://openalex.org/F4320334789","funder_display_name":"Japan Science and Technology Agency"}],"funders":[{"id":"https://openalex.org/F4320311405","display_name":"Japan Agency for Medical Research and Development","ror":"https://ror.org/004rtk039"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320334789","display_name":"Japan Science and Technology Agency","ror":"https://ror.org/00097mb19"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1587481053","https://openalex.org/W1711766424","https://openalex.org/W1988356128","https://openalex.org/W2023594790","https://openalex.org/W2091438466","https://openalex.org/W2143951116","https://openalex.org/W2768826694","https://openalex.org/W2995103612","https://openalex.org/W3008463636","https://openalex.org/W3011843929","https://openalex.org/W4212983655","https://openalex.org/W4376109039","https://openalex.org/W4378472834","https://openalex.org/W4407807922","https://openalex.org/W4411566683"],"related_works":[],"abstract_inverted_index":{"Elucidating":[0],"the":[1,27,55,71,100,104,113,148,160],"binding":[2,187],"kinetics":[3],"of":[4,17,29,73,93,103,163,165,184],"protein\u2013small":[5],"molecule":[6,186],"interactions":[7],"is":[8,23,195],"critical":[9],"for":[10,174],"optimizing":[11],"drug":[12],"efficacy":[13],"and":[14,82,178],"understanding":[15],"mechanism":[16],"action.":[18],"However,":[19],"establishing":[20],"structure\u2013kinetic":[21],"relationships":[22],"often":[24],"hindered":[25],"by":[26,128],"throughput":[28],"conditional":[30],"assays":[31],"that":[32,45,133],"require":[33],"protein":[34,48,179,193],"purification.":[35],"Herein,":[36],"we":[37,58,88,98],"present":[38],"smFASTIA,":[39],"a":[40,86,108,171],"rapid,":[41],"purification-free":[42],"screening":[43,140,183,213],"platform":[44],"integrates":[46],"cell-free":[47],"synthesis":[49],"with":[50,143,218],"high-sensitivity":[51],"bio-layer":[52],"interferometry.":[53],"Using":[54,77],"SpyTag003\u2013SpyCatcher003":[56],"system,":[57],"immobilized":[59],"target":[60],"proteins":[61],"at":[62],"high":[63,216],"density":[64],"directly":[65,197],"from":[66,198],"crude":[67],"reaction":[68,199],"mixtures,":[69],"enabling":[70],"detection":[72],"weak":[74],"small-molecule":[75],"signals.":[76],"human":[78],"carbonic":[79],"anhydrase":[80],"II":[81],"its":[83],"inhibitor":[84],"as":[85,107,136],"model,":[87],"screened":[89],"32":[90],"variants.":[91],"Instead":[92],"relying":[94],"on":[95,123,154],"equilibrium":[96],"analysis,":[97],"utilized":[99],"initial":[101],"slope":[102,203],"association":[105,114,155],"phase":[106],"kinetic":[109,125,161],"indicator":[110],"proportional":[111],"to":[112,151,208],"rate":[115],"constant.":[116],"This":[117,157],"approach":[118],"successfully":[119],"discriminated":[120],"variants":[121,134,166],"based":[122,153],"their":[124],"profiles.":[126],"Validation":[127],"surface":[129,219],"plasmon":[130,220],"resonance":[131,221],"confirmed":[132],"classified":[135],"non-binders":[137],"in":[138],"our":[139],"included":[141],"those":[142],"significantly":[144],"reduced":[145],"on-rates,":[146],"demonstrating":[147],"platform's":[149],"ability":[150],"filter":[152],"kinetics.":[156],"workflow":[158],"enables":[159],"assessment":[162],"dozens":[164],"within":[167],"two":[168],"days,":[169],"providing":[170],"powerful":[172],"tool":[173],"accelerating":[175],"SKR":[176],"studies":[177],"engineering.":[180],"\u2022":[181,191,201,211],"Purification-free":[182],"protein-small":[185],"has":[188],"been":[189],"developed.":[190],"High-density":[192],"immobilization":[194],"achieved":[196],"mixtures.":[200],"Initial":[202],"analysis":[204],"distinguishes":[205],"signals":[206],"attributable":[207],"specific":[209],"interactions.":[210],"The":[212],"results":[214],"showed":[215],"concordance":[217],"data.":[222]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2026-01-17T00:00:00"}