{"id":"https://openalex.org/W7124304242","doi":"https://doi.org/10.1371/journal.pone.0337417","title":"Lethal effect of heterologous Haemonchus contortus \u03b1- and \u03b2-tubulin expression in Leishmania tarentolae","display_name":"Lethal effect of heterologous Haemonchus contortus \u03b1- and \u03b2-tubulin expression in Leishmania tarentolae","publication_year":2026,"publication_date":"2026-01-15","ids":{"openalex":"https://openalex.org/W7124304242","doi":"https://doi.org/10.1371/journal.pone.0337417","pmid":"https://pubmed.ncbi.nlm.nih.gov/41538391"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0337417","doi":"https://doi.org/10.1371/journal.pone.0337417","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0337417","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0337417&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS One","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0337417&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006035629","display_name":"Marc Borchert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Marc Borchert","raw_affiliation_strings":["Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]},{"raw_affiliation_string":"Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019869580","display_name":"J\u00fcrgen Kr\u00fccken","orcid":"https://orcid.org/0000-0002-2842-8100","observed_orcids":["https://orcid.org/0000-0002-2842-8100"]},"institutions":[{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"J\u00fcrgen Kr\u00fccken","raw_affiliation_strings":["Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0002-2842-8100","affiliations":[{"raw_affiliation_string":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]},{"raw_affiliation_string":"Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Marion M\u00fcller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Marion M\u00fcller","raw_affiliation_strings":["Institute of Immunology, Freie Universit\u00e4t Berlin, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Immunology, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037903543","display_name":"Georg von Samson\u2010Himmelstjerna","orcid":"https://orcid.org/0000-0002-6043-2038","observed_orcids":["https://orcid.org/0000-0002-6043-2038"]},"institutions":[{"id":"https://openalex.org/I75951250","display_name":"Freie Universit\u00e4t Berlin","ror":"https://ror.org/046ak2485","country_code":"DE","type":"education","lineage":["https://openalex.org/I75951250"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Georg von Samson-Himmelstjerna","raw_affiliation_strings":["Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany"],"raw_orcid":"https://orcid.org/0000-0002-6043-2038","affiliations":[{"raw_affiliation_string":"Institute for Parasitology and Tropical Veterinary Medicine, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]},{"raw_affiliation_string":"Veterinary Centre for Resistance Research, Freie Universit\u00e4t Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I75951250"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I75951250"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.02943135,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"1","first_page":"e0337417","last_page":"e0337417"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":0.5702999830245972,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":0.5702999830245972,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.16459999978542328,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.04190000146627426,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/haemonchus-contortus","display_name":"Haemonchus contortus","score":0.9950000047683716},{"id":"https://openalex.org/keywords/beta-tubulin","display_name":"beta-tubulin","score":0.8109999895095825},{"id":"https://openalex.org/keywords/alpha-tubulin","display_name":"alpha-tubulin","score":0.9909999966621399},{"id":"https://openalex.org/keywords/leishmania-tarentolae","display_name":"Leishmania tarentolae","score":0.949999988079071},{"id":"https://openalex.org/keywords/benzimidazole-anthelmintics","display_name":"benzimidazole anthelmintics","score":0.8109999895095825},{"id":"https://openalex.org/keywords/microtubule-disruption","display_name":"microtubule disruption","score":0.8629999756813049}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8851000070571899},{"id":"https://openalex.org/C2779825395","wikidata":"https://www.wikidata.org/wiki/Q1754336","display_name":"Haemonchus contortus","level":3,"score":0.7864000201225281},{"id":"https://openalex.org/C84425145","wikidata":"https://www.wikidata.org/wiki/Q422492","display_name":"Tubulin","level":3,"score":0.6567999720573425},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.6365000009536743},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.5993000268936157},{"id":"https://openalex.org/C20418707","wikidata":"https://www.wikidata.org/wiki/Q189933","display_name":"Microtubule","level":2,"score":0.5200999975204468},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.5109000205993652},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4986000061035156},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4672999978065491},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4399000108242035},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4361000061035156},{"id":"https://openalex.org/C2778830712","wikidata":"https://www.wikidata.org/wiki/Q5185","display_name":"Nematode","level":2,"score":0.4350999891757965},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.40220001339912415},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.33660000562667847},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.3361999988555908},{"id":"https://openalex.org/C2778944004","wikidata":"https://www.wikidata.org/wiki/Q91703","display_name":"Caenorhabditis elegans","level":3,"score":0.31349998712539673},{"id":"https://openalex.org/C58207958","wikidata":"https://www.wikidata.org/wiki/Q33596","display_name":"Motility","level":2,"score":0.3124000132083893},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.2989000082015991},{"id":"https://openalex.org/C2781333301","wikidata":"https://www.wikidata.org/wiki/Q1950752","display_name":"Leishmania donovani","level":4,"score":0.2793999910354614},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.2791999876499176},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.27709999680519104},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2727000117301941},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.2687000036239624},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.2685000002384186},{"id":"https://openalex.org/C2781421772","wikidata":"https://www.wikidata.org/wiki/Q581102","display_name":"Antiparasitic","level":2,"score":0.2678000032901764},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.26010000705718994},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.26010000705718994},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.25870001316070557},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.2572999894618988},{"id":"https://openalex.org/C2775848585","wikidata":"https://www.wikidata.org/wiki/Q139658","display_name":"Brugia malayi","level":4,"score":0.257099986076355}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006189","descriptor_name":"Haemonchus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007891","descriptor_name":"Leishmania","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014404","descriptor_name":"Tubulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1371/journal.pone.0337417","doi":"https://doi.org/10.1371/journal.pone.0337417","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0337417","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0337417&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS One","raw_type":"journal-article"},{"id":"pmid:41538391","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41538391","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12806841","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12806841/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12806841/pdf/pone.0337417.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:a14f672972dc44d29c5815a61b96e68f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/a14f672972dc44d29c5815a61b96e68f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 21, Iss 1, p e0337417 (2026)","raw_type":"article"},{"id":"pmh:oai:refubium.fu-berlin.de:fub188/51395","doi":null,"is_oa":true,"landing_page_url":"https://refubium.fu-berlin.de/handle/fub188/51395","pdf_url":null,"source":{"id":"https://openalex.org/S4306402444","display_name":"Refubium (Universit\u00e4tsbibliothek der Freien Universit\u00e4t Berlin)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I75951250","host_organization_name":"Freie Universit\u00e4t Berlin","host_organization_lineage":["https://openalex.org/I75951250"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doc-type:article"},{"id":"doi:10.17169/refubium-51120","doi":"https://doi.org/10.17169/refubium-51120","is_oa":true,"landing_page_url":"https://doi.org/10.17169/refubium-51120","pdf_url":null,"source":{"id":"https://openalex.org/S7407052913","display_name":"Universit\u00e4tsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0337417","doi":"https://doi.org/10.1371/journal.pone.0337417","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0337417","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0337417&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS One","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2372340310","display_name":"Funktionale Analyse und Evidenzbasierte Ans\u00e4tze zur Vermeidung von BenzimidazolResistenz bei Haemonchus spp.","funder_award_id":"411112607","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7124304242.pdf","grobid_xml":"https://content.openalex.org/works/W7124304242.grobid-xml"},"referenced_works_count":39,"referenced_works":["https://openalex.org/W1560760512","https://openalex.org/W1967609695","https://openalex.org/W1973713316","https://openalex.org/W1993691117","https://openalex.org/W1999730565","https://openalex.org/W2007901975","https://openalex.org/W2014179603","https://openalex.org/W2017117289","https://openalex.org/W2027238676","https://openalex.org/W2032148546","https://openalex.org/W2037094686","https://openalex.org/W2037196594","https://openalex.org/W2041327979","https://openalex.org/W2046006262","https://openalex.org/W2048620082","https://openalex.org/W2059037153","https://openalex.org/W2078949445","https://openalex.org/W2081219537","https://openalex.org/W2088551598","https://openalex.org/W2102828449","https://openalex.org/W2110403693","https://openalex.org/W2112453382","https://openalex.org/W2117626458","https://openalex.org/W2130122714","https://openalex.org/W2141609688","https://openalex.org/W2344733175","https://openalex.org/W2470282944","https://openalex.org/W2498870949","https://openalex.org/W2554898533","https://openalex.org/W2790381688","https://openalex.org/W2901583425","https://openalex.org/W2970251761","https://openalex.org/W3009111017","https://openalex.org/W3094756542","https://openalex.org/W3113214096","https://openalex.org/W3202033919","https://openalex.org/W4224295886","https://openalex.org/W4226140689","https://openalex.org/W4322758674"],"related_works":[],"abstract_inverted_index":{"Benzimidazoles":[0],"are":[1],"widely":[2],"used":[3],"anthelmintics":[4],"that":[5,109,148,177],"target":[6],"\u03b2-tubulin":[7,40],"and":[8,18,38,47,96,99,128,140,175],"disrupt":[9],"microtubule":[10,133],"formation,":[11],"yet":[12],"the":[13,29,43,103,131,160,188],"precise":[14],"mode":[15],"of":[16,33,60,111,130,138,163,191],"action":[17],"resistance":[19],"mechanisms":[20],"in":[21,136],"parasitic":[22],"nematodes":[23],"remains":[24],"incompletely":[25],"understood.":[26],"To":[27],"investigate":[28],"processes,":[30],"heterologous":[31,172],"expression":[32,151,174,182],"Haemonchus":[34],"contortus":[35,114],"isotype-1":[36,39],"\u03b1-tubulin":[37],"linked":[41],"to":[42,76,118,123,156,171],"selection":[44],"markers":[45],"nourseothricin":[46],"hygromycin,":[48],"respectively,":[49],"was":[50,64],"attempted":[51],"following":[52],"integration":[53],"into":[54],"Leishmania":[55],"tarentolae":[56,74],"ssu(18S)":[57],"loci.":[58],"Transcription":[59],"both":[61],"tubulin":[62,150,164,173],"genes":[63],"confirmed":[65],"by":[66],"RT-PCR":[67],"two":[68],"days":[69],"post-transfection.":[70],"However,":[71],"transgenic":[72],"L.":[73,119],"failed":[75],"recover":[77],"or":[78,179],"enter":[79],"exponential":[80],"growth":[81],"under":[82],"antibiotic":[83],"selection.":[84],"In":[85],"contrast,":[86],"control":[87],"constructs":[88],"containing":[89],"scrambled":[90],"open":[91],"reading":[92],"frame":[93],"integrated":[94],"successfully":[95],"yielded":[97],"viable":[98],"proliferating":[100],"lines":[101],"expressing":[102],"corresponding":[104],"mRNAs.":[105],"These":[106],"findings":[107],"indicate":[108],"translation":[110],"functional":[112,161,189],"H.":[113],"tubulins":[115,127],"is":[116],"toxic":[117],"tarentolae,":[120],"likely":[121],"due":[122,155],"incompatibility":[124,167],"with":[125],"native":[126,132],"disruption":[129],"network,":[134],"resulting":[135],"loss":[137],"motility":[139],"cell":[141],"death.":[142],"The":[143],"study":[144],"provides":[145],"experimental":[146],"evidence":[147],"cross-species":[149],"can":[152],"be":[153,185],"lethal":[154],"cytoskeletal":[157],"interference,":[158],"underscoring":[159],"specificity":[162],"subunits.":[165],"This":[166],"represents":[168],"a":[169],"barrier":[170],"suggests":[176],"controlled":[178],"inducible":[180],"eukaryotic":[181],"systems":[183],"may":[184],"necessary":[186],"for":[187],"production":[190],"nematode":[192],"tubulins.":[193]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2026-01-16T00:00:00"}