{"id":"https://openalex.org/W7096831481","doi":"https://doi.org/10.1101/gad.13.24.3191","title":"Targeted mRNA degradation by double-stranded RNA in vitro","display_name":"Targeted mRNA degradation by double-stranded RNA in vitro","publication_year":1999,"publication_date":"1999-12-15","ids":{"openalex":"https://openalex.org/W7096831481","doi":"https://doi.org/10.1101/gad.13.24.3191","pmid":"https://pubmed.ncbi.nlm.nih.gov/10617568","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/317199"},"language":"en","primary_location":{"id":"doi:10.1101/gad.13.24.3191","doi":"https://doi.org/10.1101/gad.13.24.3191","is_oa":true,"landing_page_url":"https://doi.org/10.1101/gad.13.24.3191","pdf_url":"http://genesdev.cshlp.org/content/13/24/3191.full.pdf","source":{"id":"https://openalex.org/S168778273","display_name":"Genes & Development","issn_l":"0890-9369","issn":["0890-9369","1549-5477"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes &amp; Development","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"http://genesdev.cshlp.org/content/13/24/3191.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042084987","display_name":"Thomas Tuschl","orcid":"https://orcid.org/0000-0002-6483-5678","observed_orcids":["https://orcid.org/0000-0002-6483-5678"]},"institutions":[{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. Tuschl","raw_affiliation_strings":["The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. ttuschl@mpibpc.gwdg.de"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. ttuschl@mpibpc.gwdg.de","institution_ids":["https://openalex.org/I4210157710"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020225652","display_name":"Phillip D. Zamore","orcid":"https://orcid.org/0000-0002-4505-9618","observed_orcids":["https://orcid.org/0000-0002-4505-9618"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. D. Zamore","raw_affiliation_strings":["The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I4210086933","https://openalex.org/I4210157710","https://openalex.org/I57206974","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052153816","display_name":"Ruth Lehmann","orcid":"https://orcid.org/0000-0002-8454-5651","observed_orcids":["https://orcid.org/0000-0002-8454-5651"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Lehmann","raw_affiliation_strings":["The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I4210086933","https://openalex.org/I4210157710","https://openalex.org/I57206974","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087589263","display_name":"David P. Bartel","orcid":"https://orcid.org/0000-0002-3872-2856","observed_orcids":["https://orcid.org/0000-0002-3872-2856"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. P. Bartel","raw_affiliation_strings":["The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I4210086933","https://openalex.org/I4210157710","https://openalex.org/I57206974","https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022945869","display_name":"Phillip A. Sharp","orcid":"https://orcid.org/0000-0003-1465-1691","observed_orcids":["https://orcid.org/0000-0003-1465-1691"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I4210157710","display_name":"Whitehead Institute for Biomedical Research","ror":"https://ror.org/04vqm6w82","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210157710","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. A. Sharp","raw_affiliation_strings":["The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA; \n                           Center for Cancer Research and \n                           Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA; \n                           Howard Hughes Medical Institute and The Skirball Institute, New York University Medical Center, New York, New York 10016,\n                           USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I4210086933","https://openalex.org/I4210157710","https://openalex.org/I57206974","https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":843,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"13","issue":"24","first_page":"3191","last_page":"3197"},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.24629999697208405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.24629999697208405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.12229999899864197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.08259999752044678,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/double-stranded-rna","display_name":"double-stranded RNA","score":0.9020000100135803},{"id":"https://openalex.org/keywords/in-vitro-rna-synthesis","display_name":"in vitro RNA synthesis","score":0.5590000152587891}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8672000169754028},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.8374000191688538},{"id":"https://openalex.org/C2191507","wikidata":"https://www.wikidata.org/wiki/Q7277185","display_name":"RNA silencing","level":5,"score":0.8241999745368958},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6453999876976013},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6161999702453613},{"id":"https://openalex.org/C98535976","wikidata":"https://www.wikidata.org/wiki/Q4088022","display_name":"Blastoderm","level":4,"score":0.5659999847412109},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5504000186920166},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5098999738693237},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.49549999833106995},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4715000092983246},{"id":"https://openalex.org/C155330019","wikidata":"https://www.wikidata.org/wiki/Q21106117","display_name":"RNA-induced silencing complex","level":5,"score":0.4049000144004822},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.38929998874664307},{"id":"https://openalex.org/C172827444","wikidata":"https://www.wikidata.org/wiki/Q7277128","display_name":"RNA-induced transcriptional silencing","level":5,"score":0.382099986076355},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3675999939441681},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.3515999913215637},{"id":"https://openalex.org/C32022120","wikidata":"https://www.wikidata.org/wiki/Q797225","display_name":"Interference (communication)","level":3,"score":0.32359999418258667},{"id":"https://openalex.org/C139278185","wikidata":"https://www.wikidata.org/wiki/Q16335222","display_name":"DNA-directed RNA interference","level":5,"score":0.32260000705718994},{"id":"https://openalex.org/C60723119","wikidata":"https://www.wikidata.org/wiki/Q7833577","display_name":"Trans-acting siRNA","level":5,"score":0.30250000953674316},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.28999999165534973},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2759000062942505},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.265500009059906}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001756","descriptor_name":"Blastoderm","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004331","descriptor_name":"Drosophila melanogaster","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":true},{"descriptor_ui":"D004331","descriptor_name":"Drosophila melanogaster","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012330","descriptor_name":"RNA, Double-Stranded","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012330","descriptor_name":"RNA, Double-Stranded","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014157","descriptor_name":"Transcription Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D020413","descriptor_name":"3' Untranslated Regions","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029721","descriptor_name":"Drosophila Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1101/gad.13.24.3191","doi":"https://doi.org/10.1101/gad.13.24.3191","is_oa":true,"landing_page_url":"https://doi.org/10.1101/gad.13.24.3191","pdf_url":"http://genesdev.cshlp.org/content/13/24/3191.full.pdf","source":{"id":"https://openalex.org/S168778273","display_name":"Genes & Development","issn_l":"0890-9369","issn":["0890-9369","1549-5477"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes &amp; Development","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:317199","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/317199","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes Dev","raw_type":"Text"},{"id":"pmid:10617568","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10617568","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes & development","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.315.7065","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.315.7065","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://bartellab.wi.mit.edu/publication_reprints/Tuschl_GenesDev99.pdf","raw_type":"text"},{"id":"pmh:oai:edoc.mpg.de:240139","doi":null,"is_oa":false,"landing_page_url":"http://edoc.mpg.de/240139","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Genes &amp; Development, v.13, 3191-3197 (1999)","raw_type":"Article"},{"id":"pmh:oai:pure.mpg.de:item_600675","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11858/00-001M-0000-0012-FBE1-1","pdf_url":null,"source":{"id":"https://openalex.org/S4306400654","display_name":"MPG.PuRe (Max Planck Society)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Genes &amp; Development","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1101/gad.13.24.3191","doi":"https://doi.org/10.1101/gad.13.24.3191","is_oa":true,"landing_page_url":"https://doi.org/10.1101/gad.13.24.3191","pdf_url":"http://genesdev.cshlp.org/content/13/24/3191.full.pdf","source":{"id":"https://openalex.org/S168778273","display_name":"Genes & Development","issn_l":"0890-9369","issn":["0890-9369","1549-5477"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes &amp; Development","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7096831481.pdf","grobid_xml":"https://content.openalex.org/works/W7096831481.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W65748083","https://openalex.org/W1486450190","https://openalex.org/W1534256444","https://openalex.org/W1647075334","https://openalex.org/W1661448904","https://openalex.org/W1926787745","https://openalex.org/W1932753289","https://openalex.org/W1964958396","https://openalex.org/W1972181955","https://openalex.org/W1972937465","https://openalex.org/W1980912932","https://openalex.org/W1998159635","https://openalex.org/W2002256585","https://openalex.org/W2009207290","https://openalex.org/W2014565441","https://openalex.org/W2023447954","https://openalex.org/W2036253078","https://openalex.org/W2055357764","https://openalex.org/W2072572526","https://openalex.org/W2073824199","https://openalex.org/W2078319759","https://openalex.org/W2083663246","https://openalex.org/W2087004183","https://openalex.org/W2088673176","https://openalex.org/W2093008096","https://openalex.org/W2122068203","https://openalex.org/W2122994357","https://openalex.org/W2165558713","https://openalex.org/W2405074170","https://openalex.org/W4248440390"],"related_works":["https://openalex.org/W7096831953","https://openalex.org/W7098058951","https://openalex.org/W2008881509","https://openalex.org/W644878022","https://openalex.org/W4244646232","https://openalex.org/W2341793514","https://openalex.org/W7113676495","https://openalex.org/W2796544595","https://openalex.org/W2416647570","https://openalex.org/W2406818832"],"abstract_inverted_index":{"Double-stranded":[0],"RNA":[1],"(dsRNA)":[2],"directs":[3],"gene-specific,":[4],"post-transcriptional":[5],"silencing":[6],"in":[7,57,101],"many":[8,48],"organisms,":[9],"including":[10],"vertebrates,":[11],"and":[12,76],"has":[13],"provided":[14],"a":[15,38,78],"new":[16],"tool":[17],"for":[18],"studying":[19],"gene":[20],"function.":[21],"The":[22,54],"biochemical":[23],"mechanisms":[24],"underlying":[25],"this":[26,58],"dsRNA":[27,66,86],"interference":[28,55],"(RNAi)":[29],"are":[30],"unknown.":[31],"Here":[32],"we":[33],"report":[34],"the":[35,50],"development":[36],"of":[37,49,52,81,85],"cell-free":[39],"system":[40],"from":[41],"syncytial":[42],"blastoderm":[43],"Drosophila":[44],"embryos":[45],"that":[46,93],"recapitulates":[47],"features":[51],"RNAi.":[53],"observed":[56],"reaction":[59],"is":[60,63],"sequence":[61],"specific,":[62],"promoted":[64],"by":[65,72,98],"but":[67],"not":[68],"single-stranded":[69],"RNA,":[70],"functions":[71],"specific":[73],"mRNA":[74],"degradation,":[75],"requires":[77],"minimum":[79],"length":[80],"dsRNA.":[82],"Furthermore,":[83],"preincubation":[84],"potentiates":[87],"its":[88],"activity.":[89],"These":[90],"results":[91],"demonstrate":[92],"RNAi":[94],"can":[95],"be":[96],"mediated":[97],"sequence-specific":[99],"processes":[100],"soluble":[102],"reactions.":[103]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":10},{"year":2021,"cited_by_count":14},{"year":2020,"cited_by_count":18},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":14},{"year":2017,"cited_by_count":10},{"year":2016,"cited_by_count":12},{"year":2015,"cited_by_count":16},{"year":2014,"cited_by_count":31},{"year":2013,"cited_by_count":22},{"year":2012,"cited_by_count":31}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-27T00:00:00"}
