{"id":"https://openalex.org/W7074204355","doi":null,"title":"Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant","display_name":"Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant","publication_year":2007,"publication_date":"2007-06-01","ids":{"openalex":"https://openalex.org/W7074204355"},"language":"en","primary_location":{"id":"pmh:oai:repositorium.uminho.pt:1822/7463","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1822/7463","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"other"},"volume":null,"type":"other","is_traditional":false,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://hdl.handle.net/1822/7463","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Guimar\u00e3es, Pedro M. R.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Guimar\u00e3es, Pedro M. R.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Fran\u00e7ois, J.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Fran\u00e7ois, J.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077730198","display_name":"Jean\u2010Luc Parrou","orcid":"https://orcid.org/0000-0002-2014-1500","observed_orcids":["https://orcid.org/0000-0002-2014-1500"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Parrou, J. L.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Teixeira, J. A.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Teixeira, J. A.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5073891770","display_name":"Luc\u00edlia Domingues","orcid":"https://orcid.org/0000-0003-1089-7627","observed_orcids":["https://orcid.org/0000-0003-1089-7627"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Domingues, Luc\u00edlia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5913000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5913000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.3862000107765198,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9599999785423279},{"id":"https://openalex.org/keywords/lactose-fermentation","display_name":"lactose fermentation","score":0.9829999804496765},{"id":"https://openalex.org/keywords/evolutionary-engineering","display_name":"evolutionary engineering","score":0.6460000276565552},{"id":"https://openalex.org/keywords/serial-dilution","display_name":"serial dilution","score":0.6779999732971191},{"id":"https://openalex.org/keywords/kluyveromyces-lactis","display_name":"Kluyveromyces lactis","score":0.8339999914169312},{"id":"https://openalex.org/keywords/promoter-deletion","display_name":"promoter deletion","score":0.8209999799728394},{"id":"https://openalex.org/keywords/plasmid-copy-number","display_name":"plasmid copy number","score":0.9539999961853027},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.8320000171661377},{"id":"https://openalex.org/keywords/ethanol-production","display_name":"ethanol production","score":0.7979999780654907}],"concepts":[{"id":"https://openalex.org/C2778041695","wikidata":"https://www.wikidata.org/wiki/Q6421540","display_name":"Kluyveromyces lactis","level":4,"score":0.7786999940872192},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7024000287055969},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6779999732971191},{"id":"https://openalex.org/C2778724459","wikidata":"https://www.wikidata.org/wiki/Q127900","display_name":"Lactose","level":2,"score":0.6769999861717224},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6604999899864197},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5343999862670898},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4514999985694885},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4341999888420105},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.3971000015735626},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.3880999982357025},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.3799999952316284},{"id":"https://openalex.org/C2780764520","wikidata":"https://www.wikidata.org/wiki/Q2258607","display_name":"Kluyveromyces","level":4,"score":0.3718000054359436},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.3564000129699707},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.34200000762939453},{"id":"https://openalex.org/C58598135","wikidata":"https://www.wikidata.org/wiki/Q24780812","display_name":"Lactose permease","level":5,"score":0.3386000096797943},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.33480000495910645},{"id":"https://openalex.org/C43053761","wikidata":"https://www.wikidata.org/wiki/Q286379","display_name":"Beta-galactosidase","level":4,"score":0.3167000114917755},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.29120001196861267},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.2838999927043915},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.25220000743865967}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repositorium.uminho.pt:1822/7463","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1822/7463","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"other"},{"id":"pmh:oai:repositorium.uminho.pt:1822/7975","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1822/7975","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"}],"best_oa_location":{"id":"pmh:oai:repositorium.uminho.pt:1822/7463","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1822/7463","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"other"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W92313992","https://openalex.org/W1513717105","https://openalex.org/W1551957277","https://openalex.org/W1763161027","https://openalex.org/W1764011743","https://openalex.org/W1904257689","https://openalex.org/W1908493956","https://openalex.org/W1908662736","https://openalex.org/W1948213703","https://openalex.org/W1968604794","https://openalex.org/W1980629935","https://openalex.org/W1990198788","https://openalex.org/W1990212260","https://openalex.org/W2004962297","https://openalex.org/W2005377356","https://openalex.org/W2026066874","https://openalex.org/W2030728458","https://openalex.org/W2035397505","https://openalex.org/W2043693423","https://openalex.org/W2047597963","https://openalex.org/W2053837262","https://openalex.org/W2060770770","https://openalex.org/W2073701512","https://openalex.org/W2082579794","https://openalex.org/W2086660631","https://openalex.org/W2101442503","https://openalex.org/W2102276577","https://openalex.org/W2110821951","https://openalex.org/W2115470699","https://openalex.org/W2116734717","https://openalex.org/W2120272121","https://openalex.org/W2121078941","https://openalex.org/W2121781334","https://openalex.org/W2122559587","https://openalex.org/W2128635872","https://openalex.org/W2129134180","https://openalex.org/W2131921511","https://openalex.org/W2135908280","https://openalex.org/W2137441463","https://openalex.org/W2143376835","https://openalex.org/W2148634741","https://openalex.org/W2149842777","https://openalex.org/W2162635255","https://openalex.org/W2163949203","https://openalex.org/W2169142563","https://openalex.org/W4247892585"],"related_works":["https://openalex.org/W7097143001","https://openalex.org/W2257047657","https://openalex.org/W2172060270","https://openalex.org/W4283368348","https://openalex.org/W1997467072","https://openalex.org/W2026066874","https://openalex.org/W1990198788","https://openalex.org/W53847938","https://openalex.org/W2222472523","https://openalex.org/W2012627794"],"abstract_inverted_index":{"The":[0,125,245],"construction":[1],"of":[2,32,110,138,141,159,171,174,193,200,237,260],"Saccharomyces":[3],"cerevisiae":[4,21],"strains":[5],"that":[6,68,86,120,128,152,168,222],"ferment":[7],"lactose":[8,43,60,70,146,229],"has":[9],"biotechnological":[10],"interest,":[11],"particularly":[12],"for":[13,203,257],"cheese":[14,251],"whey":[15],"fermentation.":[16,44],"A":[17],"flocculent":[18],"lactose-consuming":[19],"S.":[20],"recombinant":[22,66,150,182],"expressing":[23],"the":[24,78,88,92,99,111,122,129,136,139,148,153,156,163,169,172,175,180,186,189,197,209,227,235,258],"LAC12":[25,106,144,206],"(lactose":[26],"permease)":[27],"and":[28,57,105,107,143,151,196,205],"LAC4":[29,104,142,204],"(\u03b2-galactosidase)":[30],"genes":[31,161,178,195],"Kluyveromyces":[33],"lactis":[34],"was":[35,47,132,183],"constructed":[36],"previously":[37],"but":[38],"showed":[39],"poor":[40],"efficiency":[41],"in":[42,59,91,98,121,147,162,179,242],"This":[45,232],"strain":[46,67,243,247],"therefore":[48],"subjected":[49],"to":[50,119,134,226],"an":[51,64,254],"evolutionary":[52,239],"engineering":[53,240],"process":[54],"(serial":[55],"transfer":[56],"dilution":[58],"medium),":[61],"which":[62],"yielded":[63],"evolved":[65,93,164,181,246],"consumed":[69],"twofold":[71],"faster,":[72],"producing":[73],"30%":[74],"more":[75],"ethanol":[76],"than":[77],"original":[79,123,149],"recombinant.":[80,124],"We":[81,166],"identified":[82],"two":[83],"molecular":[84],"events":[85],"targeted":[87],"LAC":[89,177],"construct":[90],"strain:":[94],"a":[95,108],"1,593-bp":[96],"deletion":[97,154],"intergenic":[100],"region":[101],"(promoter)":[102],"between":[103,188],"decrease":[109],"plasmid":[112],"copy":[113,191],"number":[114,192],"by":[115,145,185],"about":[116],"10-fold":[117],"compared":[118],"results":[126,215],"suggest":[127],"intact":[130],"promoter":[131,211],"unable":[133],"mediate":[135],"induction":[137,158,202],"transcription":[140],"established":[155],"transcriptional":[157,201],"both":[160,194],"strain.":[165],"propose":[167],"tuning":[170],"expression":[173],"heterologous":[176],"accomplished":[184],"interplay":[187],"decreased":[190],"different":[198],"levels":[199],"resulting":[207],"from":[208],"changed":[210],"structure.":[212],"Nevertheless,":[213],"our":[214],"do":[216],"not":[217],"exclude":[218],"other":[219],"possible":[220],"mutations":[221],"may":[223],"have":[224],"contributed":[225],"improved":[228],"fermentation":[230,259],"phenotype.":[231],"study":[233],"illustrates":[234],"usefulness":[236],"simple":[238],"approaches":[241],"improvement.":[244],"efficiently":[248],"fermented":[249],"threefold-concentrated":[250],"whey,":[252],"providing":[253],"attractive":[255],"alternative":[256],"lactose-based":[261],"media.":[262]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2025-10-10T00:00:00"}